Pastor Ada 0000-0002-7114-770X Universitat de Girona UdG https://ror.org/01xdxns91 Holmboe Cecilie Marie Hartvig cecilie.holmboe@bio.au.dk 0009-0005-5842-1857 Aarhus University AU http://www.au.dk/en/ https://ror.org/01aj84f44 Han Xingguo xingguo.han@wsl.ch WSL Institute for Snow and Avalanche Research SLF https://ror.org/04pzmmv39 Frossard Aline aline.frossard@wsl.ch 0000-0003-1699-6220 WSL Institute for Snow and Avalanche Research SLF https://ror.org/04pzmmv39 Romaní Anna M Universitat de Girona UdG https://ror.org/01xdxns91 Kjær Jakob B jbk@bio.au.dk Aarhus University AU http://www.au.dk/en/ https://ror.org/01aj84f44 Tank Jennifer jtank@nd.edu UNIV NOTRE DAME https://ror.org/00mkhxb43 Giménez-Grau Pau pgimenezgrau@gmail.com Catalan Institute for Water Research ICRA http://www.icra.cat/ https://ror.org/04zfaj906 Hille Erika Erika.Hille@auroracollege.nt.ca Aurora College http://www.auroracollege.nt.ca/ https://ror.org/05bn6qf70 D'Acqui Luigi P luigipaolo.dacqui@cnr.it Consiglio Nazionale delle Ricerche - Istituto di Ricerca sugli Ecosistemi Terrestri CNR IRET https://www.iret.cnr.it https://ror.org/00keh9s20 Catalán Núria nuria.catalan@ceab.csic.es Centre d'Estudis Avançats de Blanes https://ror.org/019pzjm43 Riis Tenna tenna.riis@bio.au.dk 0000-0003-2501-4444 Aarhus University AU http://www.au.dk/en/ https://ror.org/01aj84f44 2026 Microbial Nitrogen Cycling Genes in Arctic Streams dataset https://doi.org/10.1594/PANGAEA.988290 2026-02-09T12:23:26 This study integrates molecular, environmental, and spatiotemporal data to examine the structure and function of streambed microbial communities across Arctic regions experiencing rapid climate change. Sediment samples were collected from 28 streams spanning four Arctic regions during summer 2021, including 15 Greenlandic streams that were sampled three times throughout the open-water season to capture temporal variation. Microbial community composition was characterized using 16S rRNA gene sequencing, while functional potential for nitrogen cycling was quantified through qPCR targeting six key genes involved in N-fixation (nifH), nitrification (amoA, nxrB), and denitrification (nirS, norB, nosZ). Environmental data included catchment characteristics, streamwater and sediment caractheristics. These molecular and environmental datasets were integrated with spatial and temporal sampling metadata to assess biogeographic and seasonal trends. Together, these data provide a comprehensive framework for understanding how Arctic stream microbial communities and nitrogen cycling functions respond to spatial heterogeneity and seasonal dynamics under ongoing climate change. Holmboe Cecilie Marie Hartvig cecilie.holmboe@bio.au.dk 0009-0005-5842-1857 Riis Tenna tenna.riis@bio.au.dk 0000-0003-2501-4444 Han Xingguo xingguo.han@wsl.ch Frossard Aline aline.frossard@wsl.ch 0000-0003-1699-6220 Romaní Anna M Kjær Jakob B jbk@bio.au.dk Tank Jennifer jtank@nd.edu Giménez-Grau Pau pgimenezgrau@gmail.com Hille Erika Erika.Hille@auroracollege.nt.ca D'Acqui Luigi P luigipaolo.dacqui@cnr.it Catalán Núria nuria.catalan@ceab.csic.es Pastor Ada 0000-0002-7114-770X accepted Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams Global Biogeochemical Cycles Holmboe Cecilie Marie Hartvig cecilie.holmboe@bio.au.dk 0009-0005-5842-1857 Pastor Ada 0000-0002-7114-770X Riis Tenna tenna.riis@bio.au.dk 0000-0003-2501-4444 2024 Stream Nitrogen Concentrations Across Arctic Vegetation Gradients journal article Global Biogeochemical Cycles 38(2) https://doi.org/10.1029/2023GB007840 e2023GB007840 -149.5927084 12.00748 67.75333333 78.93631 -49.89087355 73.52708179428573 2021-07-09T00:00:00 2021-09-17T00:00:00 5.718027592 890.36 Aarhus University AU http://www.au.dk/en/ https://doi.org/10.13039/100007605 AUFF-E-2020-9-16 Deciphering the role of rocks as nitrogen source for biological cycling in Arctic freshwaters (RockNrole) Danish National Research Foundation DG http://dg.dk/en/ https://doi.org/10.13039/501100001732 DNRF185 Center for Ice-Free Arctic Research (CIFAR) https://bio.au.dk/forskning/forskningscentre/cifar Horizon 2020 H2020 https://ec.europa.eu/programmes/horizon2020/ https://doi.org/10.13039/501100007601 730938 Nitrogen sources for Arctic freshwaters (NitroFresh) Horizon 2020 H2020 https://ec.europa.eu/programmes/horizon2020/ https://doi.org/10.13039/501100007601 839709 Linking Chemical diversity and Reactivity of Arctic dissolved Organic Matter for its integration in Earth system model https://doi.org/10.3030/839709 Spanish Ministry of Science, Innovation and Universities MICINN https://www.ciencia.gob.es https://doi.org/10.13039/501100004837 RYC2022-036661-I Villum Fonden https://veluxfoundations.dk https://doi.org/10.13039/100008398 00028351 Nitrogen in rocks: an overlooked nutrient source in Arctic aquatic ecosystems https://pure.au.dk/portal/en/projects/nitrogen-in-rocks-an-overlooked-nutrient-source-in-arctic-aquatic/ Aucella_microbes 74.50327 -20.54179 153.924469 2021-07-18T00:00:00 2021-09-12T00:00:00 Northeast Greenland Multiple investigations MULT Austre-Bayelva_microbes 78.91994 11.82625 52.54 2021-08-23T00:00:00 Svalbard Multiple investigations MULT Bayelva_microbes 78.92032 11.79589 50.41 2021-08-27T00:00:00 Svalbard Multiple investigations MULT Caribou_Creek_microbes 68.08722222 -133.4922222 19.57 2021-08-24T00:00:00 Inuvik region, Canada Multiple investigations MULT Grænseelv_microbes 74.47321 -20.50832 23.01326561 2021-07-19T00:00:00 2021-09-15T00:00:00 Northeast Greenland Multiple investigations MULT Havipak_Creek_microbes 68.31295833 -133.5235167 48.49 2021-08-19T00:00:00 Inuvik region, Canada Multiple investigations MULT Imnavait_microbes 68.62499523 -149.3246583 864.288 2021-08-24T00:00:00 Alaska North Slope Multiple investigations MULT Jurassic_1_microbes 74.43217 -20.28988 59.4781189 2021-07-17T00:00:00 2021-08-14T00:00:00 Northeast Greenland Multiple investigations MULT Jurassic_2_microbes 74.43983 -20.30799 106.3590317 2021-07-17T00:00:00 2021-08-14T00:00:00 Northeast Greenland Multiple investigations MULT Kærelv_A_microbes 74.49468 -20.49629 189.7179718 2021-07-10T00:00:00 2021-09-08T00:00:00 Northeast Greenland Multiple investigations MULT Kærelv_C_microbes 74.47102 -20.51882 14.02111626 2021-07-09T00:00:00 2021-09-08T00:00:00 Northeast Greenland Multiple investigations MULT Kuparuk_microbes 68.64075024 -149.3998278 748.07 2021-08-25T00:00:00 Alaska North Slope Multiple investigations MULT Lindemann_tributary_microbes 74.53919 -20.65342 181.5577698 2021-07-15T00:00:00 2021-08-09T00:00:00 Northeast Greenland Multiple investigations MULT Mørebekken_microbes 78.93631 11.82406 49.0 2021-08-25T00:00:00 Svalbard Multiple investigations MULT Oksrukuyik_microbes 68.68583542 -149.1003349 761.75 2021-08-26T00:00:00 Alaska North Slope Multiple investigations MULT Palnatoke_microbes 74.52727 -20.5752 212.6996155 2021-07-15T00:00:00 2021-08-09T00:00:00 Northeast Greenland Multiple investigations MULT Rengleng_River_microbes 67.75333333 -133.8625 33.54 2021-08-24T00:00:00 Inuvik region, Canada Multiple investigations MULT Storvatenet_microbes 78.92285 11.88603 55.73 2021-08-21T00:00:00 Svalbard Multiple investigations MULT Toolik_Inlet_microbes 68.62513162 -149.5927084 722.78 2021-08-21T00:00:00 Alaska North Slope Multiple investigations MULT Tørvedammen_microbes 74.47926 -20.55715 36.64139557 2021-07-14T00:00:00 2021-09-12T00:00:00 Northeast Greenland Multiple investigations MULT Trevor_microbes 68.28343385 -149.3716111 890.36 2021-07-09T00:00:00 Alaska North Slope Multiple investigations MULT Voitelva_microbes 78.90658 12.00748 63.35 2021-08-28T00:00:00 Svalbard Multiple investigations MULT West_1_microbes 74.47394 -20.60757 35.19841766 2021-07-11T00:00:00 2021-09-12T00:00:00 Northeast Greenland Multiple investigations MULT West_2_microbes 74.47158 -20.65557 118.6093369 2021-07-13T00:00:00 2021-09-13T00:00:00 Northeast Greenland Multiple investigations MULT West_3_microbes 74.46048 -20.69274 13.04383278 2021-07-13T00:00:00 2021-09-13T00:00:00 Northeast Greenland Multiple investigations MULT West_4_microbes 74.44862 -20.7854 9.384347916 2021-08-12T00:00:00 2021-09-10T00:00:00 Northeast Greenland Multiple investigations MULT West_5_microbes 74.45419 -20.85248 5.718027592 2021-08-12T00:00:00 2021-09-10T00:00:00 Northeast Greenland Multiple investigations MULT Zackenberg_River_microbes 74.46992 -20.57417 30.36580467 2021-09-17T00:00:00 Northeast Greenland Multiple investigations MULT 1477 CC-BY-SA-4.0 Creative Commons Attribution-ShareAlike 4.0 International https://creativecommons.org/licenses/by-sa/4.0/ Event label Event Pastor Ada 0000-0002-7114-770X Event Sample code/label Sample label Pastor Ada 0000-0002-7114-770X Sample label Latitude of event Latitude Pastor Ada 0000-0002-7114-770X Latitude Longitude of event Longitude Pastor Ada 0000-0002-7114-770X Longitude DATE/TIME Date/Time http://en.wikipedia.org/wiki/ISO_8601 Time T http://dbpedia.org/resource/Time Pastor Ada 0000-0002-7114-770X Date/Time Elevation of event Elevation m Pastor Ada 0000-0002-7114-770X Elevation [m] pH pH Pastor Ada 0000-0002-7114-770X streamwater pH (streamwater) Temperature, water Temp °C Temperature Θ http://dbpedia.org/resource/Temperature water http://purl.obolibrary.org/obo/CHEBI_15377 Pastor Ada 0000-0002-7114-770X streamwater Temp [°C] (streamwater) Conductivity, electrical EC µS/cm conductivity http://purl.obolibrary.org/obo/PATO_0001585 Pastor Ada 0000-0002-7114-770X streamwater EC [µS/cm] (streamwater) Ammonium [NH4]+ mg/l ammonium http://purl.obolibrary.org/obo/CHEBI_28938 Density http://dbpedia.org/resource/Density Pastor Ada 0000-0002-7114-770X streamwater [NH4]+ [mg/l] (streamwater) Nitrate [NO3]- mg/l nitrate http://purl.obolibrary.org/obo/CHEBI_17632 Density http://dbpedia.org/resource/Density Pastor Ada 0000-0002-7114-770X streamwater [NO3]- [mg/l] (streamwater) Carbon, organic, dissolved DOC mg/l carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 organic dissolved http://purl.obolibrary.org/obo/PATO_0001986 Density http://dbpedia.org/resource/Density Pastor Ada 0000-0002-7114-770X streamwater DOC [mg/l] (streamwater) Nitrogen, organic, dissolved DON mg/l nitrogen N https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25555 organic dissolved http://purl.obolibrary.org/obo/PATO_0001986 Density http://dbpedia.org/resource/Density Pastor Ada 0000-0002-7114-770X streamwater DON [mg/l] (streamwater) Nitrogen, total dissolved TDN mg/l nitrogen N https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25555 Density http://dbpedia.org/resource/Density Pastor Ada 0000-0002-7114-770X streamwater TDN [mg/l] (streamwater) pH pH Pastor Ada 0000-0002-7114-770X sediment pH (sediment) Conductivity, electrical EC µS/cm conductivity http://purl.obolibrary.org/obo/PATO_0001585 Pastor Ada 0000-0002-7114-770X sediment EC [µS/cm] (sediment) Size fraction > 2 mm, gravel >2 mm % size http://purl.obolibrary.org/obo/PATO_0000117 Fraction gravel http://purl.obolibrary.org/obo/ENVO_01000018 Dimensionless Ratio ψ Pastor Ada 0000-0002-7114-770X Sediment size >2000 m >2 mm [%] (Sediment size >2000 m) Size fraction 2.000-0.630 mm, coarse sand 2000-630 µm % size http://purl.obolibrary.org/obo/PATO_0000117 Fraction Dimensionless Ratio ψ Pastor Ada 0000-0002-7114-770X Sediment size 2000-630 m 2000-630 µm [%] (Sediment size 2000-630 m) Size fraction 0.630-0.200 mm, medium sand 630-200 µm % size http://purl.obolibrary.org/obo/PATO_0000117 Fraction Dimensionless Ratio ψ Pastor Ada 0000-0002-7114-770X Sediment size 630-200 m 630-200 µm [%] (Sediment size 630-200 m) Size fraction 0.200-0.063 mm, fine sand 200-63 µm fS % size http://purl.obolibrary.org/obo/PATO_0000117 Fraction Dimensionless Ratio ψ Pastor Ada 0000-0002-7114-770X Sediment size 200 63 m 200-63 µm fS [%] (Sediment size 200 63 m) Loss on ignition LOI % http://en.wikipedia.org/wiki/Loss_on_ignition loss Dimensionless Ratio ψ Pastor Ada 0000-0002-7114-770X LOI [%] Ammonium [NH4]+ mg/l ammonium http://purl.obolibrary.org/obo/CHEBI_28938 Density http://dbpedia.org/resource/Density Pastor Ada 0000-0002-7114-770X sediment [NH4]+ [mg/l] (sediment) Nitrate [NO3]- mg/l nitrate http://purl.obolibrary.org/obo/CHEBI_17632 Density http://dbpedia.org/resource/Density Pastor Ada 0000-0002-7114-770X sediment [NO3]- [mg/l] (sediment) Nitrogen N % nitrogen N https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25555 Dimensionless Ratio ψ Pastor Ada 0000-0002-7114-770X sediment N [%] (sediment) Carbon C % carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 Dimensionless Ratio ψ Pastor Ada 0000-0002-7114-770X sediment C [%] (sediment) Normalized Difference Vegetation Index NDVI https://en.wikipedia.org/wiki/Normalized_Difference_Vegetation_Index Pastor Ada 0000-0002-7114-770X NDVI Catchment area Catch area km**2 Area http://dbpedia.org/resource/Area Pastor Ada 0000-0002-7114-770X Catch area [km**2] Slope Slope deg https://en.wikipedia.org/wiki/Grade_%28slope%29 slope http://purl.obolibrary.org/obo/ENVO_00002000 Angle http://dbpedia.org/resource/Angle Plane Angle http://dbpedia.org/resource/Plane_angle Pastor Ada 0000-0002-7114-770X Slope [deg] 16S rRNA gene, copy number, per unit sediment dry mass 16S rRNA gene/sed dm #/g Abundance per Mass Pastor Ada 0000-0002-7114-770X 16S rRNA gene/sed dm [#/g] AOA-amoA gene, copy number, per unit sediment dry mass AOA-amoA/sed dm #/g Abundance per Mass Pastor Ada 0000-0002-7114-770X Archaeal amoA gene (encoding ammonium monooxygenase) in sediment samples, quantified from extracted DNA AOA-amoA/sed dm [#/g] (Archaeal amoA gene (encoding ...) AOB-amoA gene, copy number, per unit sediment dry mass AOB-amoA/sed dm #/g Abundance per Mass Pastor Ada 0000-0002-7114-770X Bacterial amoA gene (encoding ammonium monooxygenase) in sediment samples, quantified from extracted DNA AOB-amoA/sed dm [#/g] (Bacterial amoA gene (encoding...) nifH gene, copy number, per unit sediment dry mass nifH/sed dm #/g Abundance per Mass Pastor Ada 0000-0002-7114-770X nifH gene (encoding the nitrogenase enzyme) in sediment samples, quantified from extracted DNA nifH/sed dm [#/g] (nifH gene (encoding the nitro...) nrxB gene, copy number, per unit sediment dry mass nrxB/sed dm #/g Abundance per Mass Pastor Ada 0000-0002-7114-770X nrxB gene (encoding the beta subunit of nitrite oxidoreductase) in sediment samples, quantified from extracted DNA nrxB/sed dm [#/g] (nrxB gene (encoding the beta ...) nosZ gene, copy number, per unit sediment dry mass nosZ/sed dm #/g Abundance per Mass Pastor Ada 0000-0002-7114-770X nosZ gene (encoding nitrous oxide reductase) in sediment samples, quantified from extracted DNA nosZ/sed dm [#/g] (nosZ gene (encoding nitrous o...) nirS gene, copy number, per unit sediment dry mass nirS/sed dm #/g Abundance per Mass Pastor Ada 0000-0002-7114-770X nirS gene (encoding cytochrome cd1 nitrite reductase) in sediment samples, quantified from extracted DNA nirS/sed dm [#/g] (nirS gene (encoding cytochrom...) qnorB gene, copy number, per unit sediment dry mass qnorB/sed dm #/g Abundance per Mass Pastor Ada 0000-0002-7114-770X qnorB gene (encoding quinol-dependent nitric oxide reductase ) in sediment samples, quantified from extracted DNA qnorB/sed dm [#/g] (qnorB gene (encoding quinol-d...) CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable Arctic diversity nitrogen genes Sediment stream water chemistry Watershed author101004 author101537 author101989 author101992 author101993 author104199 author106724 author113991 author113993 author113994 author113995 author87503 award2508 award4558 award4559 award4560 award4561 award4595 curlevel30 event3337636 event3337637 event3337638 event3337639 event3337640 event3337641 event3337642 event3337643 event3337644 event3337645 event3337646 event3337647 event3337648 event3337649 event3337650 event3337651 event3337652 event3337653 event3337654 event3337655 event3337656 event3337657 event3337658 event3337659 event3337660 event3337661 event3337662 event3337663 funder20475 funder20675 funder20913 funder21052 funder21473 geocode1599 geocode1600 geocode1601 geocode8128 inst15283 inst20913 inst21027 inst21401 inst22042 inst22498 inst22507 inst22508 journal6175 license22 method10667 param106968 param123255 param128544 param130429 param145233 param151 param15215 param179057 param2208 param26920 param2830 param2831 param2832 param501850 param552314 param552315 param552316 param552317 param552318 param552319 param552320 param552605 param717 param764 param790 param80167 param88101 pi101537 ref124305 ref137733 term1073162 term1073173 term1073201 term1073204 term1073482 term1073493 term1074060 term20387 term20417 term2606205 term37820 term38477 term38548 term40892 term41092 term43765 term43770 term43792 term43794 term43915 term43972 term43975 term44024 term44034 term44419 term47305 term47471 term47648 term49285 term51491 term71917 term76900