de.pangaea.dataset988290
und
dataset
PANGAEA
info@pangaea.de
https://www.pangaea.de/
information
pointOfContact
2026-04-02T12:20:06Z
https://doi.pangaea.de/10.1594/PANGAEA.988290
<gco:CharacterString>Microbial Nitrogen Cycling Genes in Arctic Streams</gco:CharacterString>
2026-02-09T12:23:26
publication
https://doi.org/10.1594/PANGAEA.988290
Pastor, Ada
https://orcid.org/0000-0002-7114-770X
information
author
Holmboe, Cecilie Marie Hartvig
cecilie.holmboe@bio.au.dk
https://orcid.org/0009-0005-5842-1857
information
author
Han, Xingguo
xingguo.han@wsl.ch
author
Frossard, Aline
aline.frossard@wsl.ch
https://orcid.org/0000-0003-1699-6220
information
author
Romaní, Anna M
author
Kjær, Jakob B
jbk@bio.au.dk
author
Tank, Jennifer
jtank@nd.edu
author
Giménez-Grau, Pau
pgimenezgrau@gmail.com
author
Hille, Erika
Erika.Hille@auroracollege.nt.ca
author
D'Acqui, Luigi P
luigipaolo.dacqui@cnr.it
author
Catalán, Núria
nuria.catalan@ceab.csic.es
author
Riis, Tenna
tenna.riis@bio.au.dk
https://orcid.org/0000-0003-2501-4444
information
author
PANGAEA
info@pangaea.de
https://www.pangaea.de/
information
publisher
tableDigital
This study integrates molecular, environmental, and spatiotemporal data to examine the structure and function of streambed microbial communities across Arctic regions experiencing rapid climate change. Sediment samples were collected from 28 streams spanning four Arctic regions during summer 2021, including 15 Greenlandic streams that were sampled three times throughout the open-water season to capture temporal variation. Microbial community composition was characterized using 16S rRNA gene sequencing, while functional potential for nitrogen cycling was quantified through qPCR targeting six key genes involved in N-fixation (nifH), nitrification (amoA, nxrB), and denitrification (nirS, norB, nosZ). Environmental data included catchment characteristics, streamwater and sediment caractheristics. These molecular and environmental datasets were integrated with spatial and temporal sampling metadata to assess biogeographic and seasonal trends. Together, these data provide a comprehensive framework for understanding how Arctic stream microbial communities and nitrogen cycling functions respond to spatial heterogeneity and seasonal dynamics under ongoing climate change.
completed
Pastor, Ada
https://orcid.org/0000-0002-7114-770X
information
principalInvestigator
Arctic
diversity
nitrogen genes
Sediment
stream
water chemistry
Watershed
theme
<gco:CharacterString>PANGAEA Keyword List</gco:CharacterString>
1995-01-01
creation
PANGAEA
info@pangaea.de
https://www.pangaea.de/
information
pointOfContact
copyright
copyright
CC-BY-SA-4.0: Creative Commons Attribution-ShareAlike 4.0 International
textTable
und
geoscientificInformation
-149.5927084
12.00748
67.75333333
78.93631
2021-07-09T00:00:00
2021-09-17T00:00:00
Event label
thematicClassification
Sample code/label
thematicClassification
Latitude of event
thematicClassification
Longitude of event
thematicClassification
DATE/TIME
thematicClassification
Elevation of event
physicalMeasurement
m
m
pH
thematicClassification
streamwater
Temperature, water
physicalMeasurement
streamwater
°C
°C
Conductivity, electrical
physicalMeasurement
streamwater
µS/cm
µS/cm
Ammonium
physicalMeasurement
streamwater
mg/l
mg/l
Nitrate
physicalMeasurement
streamwater
mg/l
mg/l
Carbon, organic, dissolved
physicalMeasurement
streamwater
mg/l
mg/l
Nitrogen, organic, dissolved
physicalMeasurement
streamwater
mg/l
mg/l
Nitrogen, total dissolved
physicalMeasurement
streamwater
mg/l
mg/l
pH
thematicClassification
sediment
Conductivity, electrical
physicalMeasurement
sediment
µS/cm
µS/cm
Size fraction > 2 mm, gravel
physicalMeasurement
Sediment size >2000 m
%
%
Size fraction 2.000-0.630 mm, coarse sand
physicalMeasurement
Sediment size 2000-630 m
%
%
Size fraction 0.630-0.200 mm, medium sand
physicalMeasurement
Sediment size 630-200 m
%
%
Size fraction 0.200-0.063 mm, fine sand
physicalMeasurement
Sediment size 200 63 m
%
%
Loss on ignition
physicalMeasurement
%
%
Ammonium
physicalMeasurement
sediment
mg/l
mg/l
Nitrate
physicalMeasurement
sediment
mg/l
mg/l
Nitrogen
physicalMeasurement
sediment
%
%
Carbon
physicalMeasurement
sediment
%
%
Normalized Difference Vegetation Index
thematicClassification
Catchment area
physicalMeasurement
km**2
km**2
Slope
physicalMeasurement
deg
deg
16S rRNA gene, copy number, per unit sediment dry mass
physicalMeasurement
#/g
#/g
AOA-amoA gene, copy number, per unit sediment dry mass
physicalMeasurement
Archaeal amoA gene (encoding ammonium monooxygenase) in sediment samples, quantified from extracted DNA
#/g
#/g
AOB-amoA gene, copy number, per unit sediment dry mass
physicalMeasurement
Bacterial amoA gene (encoding ammonium monooxygenase) in sediment samples, quantified from extracted DNA
#/g
#/g
nifH gene, copy number, per unit sediment dry mass
physicalMeasurement
nifH gene (encoding the nitrogenase enzyme) in sediment samples, quantified from extracted DNA
#/g
#/g
nrxB gene, copy number, per unit sediment dry mass
physicalMeasurement
nrxB gene (encoding the beta subunit of nitrite oxidoreductase) in sediment samples, quantified from extracted DNA
#/g
#/g
nosZ gene, copy number, per unit sediment dry mass
physicalMeasurement
nosZ gene (encoding nitrous oxide reductase) in sediment samples, quantified from extracted DNA
#/g
#/g
nirS gene, copy number, per unit sediment dry mass
physicalMeasurement
nirS gene (encoding cytochrome cd1 nitrite reductase) in sediment samples, quantified from extracted DNA
#/g
#/g
qnorB gene, copy number, per unit sediment dry mass
physicalMeasurement
qnorB gene (encoding quinol-dependent nitric oxide reductase ) in sediment samples, quantified from extracted DNA
#/g
#/g
text/tab-separated-values
unknown
https://doi.pangaea.de/10.1594/PANGAEA.988290?format=textfile
download
dataset
The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data.
EVENT LABEL: Aucella_microbes * LATITUDE: 74.503270 * LONGITUDE: -20.541790 * DATE/TIME START: 2021-07-18T00:00:00 * DATE/TIME END: 2021-09-12T00:00:00 * ELEVATION: 153.9 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-18T00:00:00
EVENT LABEL: Austre-Bayelva_microbes * LATITUDE: 78.919940 * LONGITUDE: 11.826250 * DATE/TIME: 2021-08-23T00:00:00 * ELEVATION: 52.5 m * LOCATION: Svalbard * METHOD/DEVICE: Multiple investigations
2021-08-23T00:00:00
EVENT LABEL: Bayelva_microbes * LATITUDE: 78.920320 * LONGITUDE: 11.795890 * DATE/TIME: 2021-08-27T00:00:00 * ELEVATION: 50.4 m * LOCATION: Svalbard * METHOD/DEVICE: Multiple investigations
2021-08-27T00:00:00
EVENT LABEL: Caribou_Creek_microbes * LATITUDE: 68.087222 * LONGITUDE: -133.492222 * DATE/TIME: 2021-08-24T00:00:00 * ELEVATION: 19.6 m * LOCATION: Inuvik region, Canada * METHOD/DEVICE: Multiple investigations
2021-08-24T00:00:00
EVENT LABEL: Grænseelv_microbes * LATITUDE: 74.473210 * LONGITUDE: -20.508320 * DATE/TIME START: 2021-07-19T00:00:00 * DATE/TIME END: 2021-09-15T00:00:00 * ELEVATION: 23.0 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-19T00:00:00
EVENT LABEL: Havipak_Creek_microbes * LATITUDE: 68.312958 * LONGITUDE: -133.523517 * DATE/TIME: 2021-08-19T00:00:00 * ELEVATION: 48.5 m * LOCATION: Inuvik region, Canada * METHOD/DEVICE: Multiple investigations
2021-08-19T00:00:00
EVENT LABEL: Imnavait_microbes * LATITUDE: 68.624995 * LONGITUDE: -149.324658 * DATE/TIME: 2021-08-24T00:00:00 * ELEVATION: 864.3 m * LOCATION: Alaska North Slope * METHOD/DEVICE: Multiple investigations
2021-08-24T00:00:00
EVENT LABEL: Jurassic_1_microbes * LATITUDE: 74.432170 * LONGITUDE: -20.289880 * DATE/TIME START: 2021-07-17T00:00:00 * DATE/TIME END: 2021-08-14T00:00:00 * ELEVATION: 59.5 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-17T00:00:00
EVENT LABEL: Jurassic_2_microbes * LATITUDE: 74.439830 * LONGITUDE: -20.307990 * DATE/TIME START: 2021-07-17T00:00:00 * DATE/TIME END: 2021-08-14T00:00:00 * ELEVATION: 106.4 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-17T00:00:00
EVENT LABEL: Kærelv_A_microbes * LATITUDE: 74.494680 * LONGITUDE: -20.496290 * DATE/TIME START: 2021-07-10T00:00:00 * DATE/TIME END: 2021-09-08T00:00:00 * ELEVATION: 189.7 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-10T00:00:00
EVENT LABEL: Kærelv_C_microbes * LATITUDE: 74.471020 * LONGITUDE: -20.518820 * DATE/TIME START: 2021-07-09T00:00:00 * DATE/TIME END: 2021-09-08T00:00:00 * ELEVATION: 14.0 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-09T00:00:00
EVENT LABEL: Kuparuk_microbes * LATITUDE: 68.640750 * LONGITUDE: -149.399828 * DATE/TIME: 2021-08-25T00:00:00 * ELEVATION: 748.1 m * LOCATION: Alaska North Slope * METHOD/DEVICE: Multiple investigations
2021-08-25T00:00:00
EVENT LABEL: Lindemann_tributary_microbes * LATITUDE: 74.539190 * LONGITUDE: -20.653420 * DATE/TIME START: 2021-07-15T00:00:00 * DATE/TIME END: 2021-08-09T00:00:00 * ELEVATION: 181.6 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-15T00:00:00
EVENT LABEL: Mørebekken_microbes * LATITUDE: 78.936310 * LONGITUDE: 11.824060 * DATE/TIME: 2021-08-25T00:00:00 * ELEVATION: 49.0 m * LOCATION: Svalbard * METHOD/DEVICE: Multiple investigations
2021-08-25T00:00:00
EVENT LABEL: Oksrukuyik_microbes * LATITUDE: 68.685835 * LONGITUDE: -149.100335 * DATE/TIME: 2021-08-26T00:00:00 * ELEVATION: 761.8 m * LOCATION: Alaska North Slope * METHOD/DEVICE: Multiple investigations
2021-08-26T00:00:00
EVENT LABEL: Palnatoke_microbes * LATITUDE: 74.527270 * LONGITUDE: -20.575200 * DATE/TIME START: 2021-07-15T00:00:00 * DATE/TIME END: 2021-08-09T00:00:00 * ELEVATION: 212.7 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-15T00:00:00
EVENT LABEL: Rengleng_River_microbes * LATITUDE: 67.753333 * LONGITUDE: -133.862500 * DATE/TIME: 2021-08-24T00:00:00 * ELEVATION: 33.5 m * LOCATION: Inuvik region, Canada * METHOD/DEVICE: Multiple investigations
2021-08-24T00:00:00
EVENT LABEL: Storvatenet_microbes * LATITUDE: 78.922850 * LONGITUDE: 11.886030 * DATE/TIME: 2021-08-21T00:00:00 * ELEVATION: 55.7 m * LOCATION: Svalbard * METHOD/DEVICE: Multiple investigations
2021-08-21T00:00:00
EVENT LABEL: Toolik_Inlet_microbes * LATITUDE: 68.625132 * LONGITUDE: -149.592708 * DATE/TIME: 2021-08-21T00:00:00 * ELEVATION: 722.8 m * LOCATION: Alaska North Slope * METHOD/DEVICE: Multiple investigations
2021-08-21T00:00:00
EVENT LABEL: Tørvedammen_microbes * LATITUDE: 74.479260 * LONGITUDE: -20.557150 * DATE/TIME START: 2021-07-14T00:00:00 * DATE/TIME END: 2021-09-12T00:00:00 * ELEVATION: 36.6 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-14T00:00:00
EVENT LABEL: Trevor_microbes * LATITUDE: 68.283434 * LONGITUDE: -149.371611 * DATE/TIME: 2021-07-09T00:00:00 * ELEVATION: 890.4 m * LOCATION: Alaska North Slope * METHOD/DEVICE: Multiple investigations
2021-07-09T00:00:00
EVENT LABEL: Voitelva_microbes * LATITUDE: 78.906580 * LONGITUDE: 12.007480 * DATE/TIME: 2021-08-28T00:00:00 * ELEVATION: 63.4 m * LOCATION: Svalbard * METHOD/DEVICE: Multiple investigations
2021-08-28T00:00:00
EVENT LABEL: West_1_microbes * LATITUDE: 74.473940 * LONGITUDE: -20.607570 * DATE/TIME START: 2021-07-11T00:00:00 * DATE/TIME END: 2021-09-12T00:00:00 * ELEVATION: 35.2 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-11T00:00:00
EVENT LABEL: West_2_microbes * LATITUDE: 74.471580 * LONGITUDE: -20.655570 * DATE/TIME START: 2021-07-13T00:00:00 * DATE/TIME END: 2021-09-13T00:00:00 * ELEVATION: 118.6 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-13T00:00:00
EVENT LABEL: West_3_microbes * LATITUDE: 74.460480 * LONGITUDE: -20.692740 * DATE/TIME START: 2021-07-13T00:00:00 * DATE/TIME END: 2021-09-13T00:00:00 * ELEVATION: 13.0 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-07-13T00:00:00
EVENT LABEL: West_4_microbes * LATITUDE: 74.448620 * LONGITUDE: -20.785400 * DATE/TIME START: 2021-08-12T00:00:00 * DATE/TIME END: 2021-09-10T00:00:00 * ELEVATION: 9.4 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-08-12T00:00:00
EVENT LABEL: West_5_microbes * LATITUDE: 74.454190 * LONGITUDE: -20.852480 * DATE/TIME START: 2021-08-12T00:00:00 * DATE/TIME END: 2021-09-10T00:00:00 * ELEVATION: 5.7 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-08-12T00:00:00
EVENT LABEL: Zackenberg_River_microbes * LATITUDE: 74.469920 * LONGITUDE: -20.574170 * DATE/TIME: 2021-09-17T00:00:00 * ELEVATION: 30.4 m * LOCATION: Northeast Greenland * METHOD/DEVICE: Multiple investigations
2021-09-17T00:00:00
Supplement to
<gco:CharacterString>Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams</gco:CharacterString>
Holmboe, Cecilie Marie Hartvig
cecilie.holmboe@bio.au.dk
https://orcid.org/0009-0005-5842-1857
information
author
Riis, Tenna
tenna.riis@bio.au.dk
https://orcid.org/0000-0003-2501-4444
information
author
Han, Xingguo
xingguo.han@wsl.ch
author
Frossard, Aline
aline.frossard@wsl.ch
https://orcid.org/0000-0003-1699-6220
information
author
Romaní, Anna M
author
Kjær, Jakob B
jbk@bio.au.dk
author
Tank, Jennifer
jtank@nd.edu
author
Giménez-Grau, Pau
pgimenezgrau@gmail.com
author
Hille, Erika
Erika.Hille@auroracollege.nt.ca
author
D'Acqui, Luigi P
luigipaolo.dacqui@cnr.it
author
Catalán, Núria
nuria.catalan@ceab.csic.es
author
Pastor, Ada
https://orcid.org/0000-0002-7114-770X
information
author
documentHardcopy
Global Biogeochemical Cycles
Related to
<gco:CharacterString>Stream Nitrogen Concentrations Across Arctic Vegetation Gradients</gco:CharacterString>
2024-01-01
publication
https://doi.org/10.1029/2023GB007840
Holmboe, Cecilie Marie Hartvig
cecilie.holmboe@bio.au.dk
https://orcid.org/0009-0005-5842-1857
information
author
Pastor, Ada
https://orcid.org/0000-0002-7114-770X
information
author
Riis, Tenna
tenna.riis@bio.au.dk
https://orcid.org/0000-0003-2501-4444
information
author
documentDigital
Global Biogeochemical Cycles
38(2)
e2023GB007840