PANGAEA_988290 Microbial Nitrogen Cycling Genes in Arctic Streams Pastor, Ada; Holmboe, Cecilie Marie Hartvig; Han, Xingguo; Frossard, Aline; Romaní, Anna M; Kjær, Jakob B; Tank, Jennifer; Giménez-Grau, Pau; Hille, Erika; D'Acqui, Luigi P; Catalán, Núria; Riis, Tenna Microbial Nitrogen Cycling Genes in Arctic Streams 2026-02-09 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.988290 Investigator Ada Pastor Earth Science Event label Sample code/label Latitude of event Longitude of event DATE/TIME Elevation of event pH Temperature, water Conductivity, electrical Ammonium Nitrate Carbon, organic, dissolved Nitrogen, organic, dissolved Nitrogen, total dissolved Size fraction > 2 mm, gravel Size fraction 2.000-0.630 mm, coarse sand Size fraction 0.630-0.200 mm, medium sand Size fraction 0.200-0.063 mm, fine sand Loss on ignition Nitrogen Carbon Normalized Difference Vegetation Index Catchment area Slope 16S rRNA gene, copy number, per unit sediment dry mass AOA-amoA gene, copy number, per unit sediment dry mass AOB-amoA gene, copy number, per unit sediment dry mass nifH gene, copy number, per unit sediment dry mass nrxB gene, copy number, per unit sediment dry mass nosZ gene, copy number, per unit sediment dry mass nirS gene, copy number, per unit sediment dry mass qnorB gene, copy number, per unit sediment dry mass geoscientificInformation Arctic Aucella_microbes Austre-Bayelva_microbes Bayelva_microbes Caribou_Creek_microbes diversity Grænseelv_microbes Havipak_Creek_microbes Imnavait_microbes Jurassic_1_microbes Jurassic_2_microbes Kærelv_A_microbes Kærelv_C_microbes Kuparuk_microbes Lindemann_tributary_microbes Multiple investigations Mørebekken_microbes nitrogen genes Oksrukuyik_microbes Palnatoke_microbes Rengleng_River_microbes Sediment Storvatenet_microbes stream Toolik_Inlet_microbes Trevor_microbes Tørvedammen_microbes Voitelva_microbes water chemistry Watershed West_1_microbes West_2_microbes West_3_microbes West_4_microbes West_5_microbes Zackenberg_River_microbes 2021-07-09 2021-09-17 Complete 67.75333333 78.93631 -149.5927084 12.00748 unrestricted CC-BY-SA-4.0: Creative Commons Attribution-ShareAlike 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 1477 data points text/tab-separated-values Holmboe, Cecilie Marie Hartvig; Riis, Tenna; Han, Xingguo; Frossard, Aline; Romaní, Anna M; Kjær, Jakob B; Tank, Jennifer; Giménez-Grau, Pau; Hille, Erika; D'Acqui, Luigi P; Catalán, Núria; Pastor, Ada (accepted): Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams. Global Biogeochemical Cycles Holmboe, Cecilie Marie Hartvig; Pastor, Ada; Riis, Tenna (2024): Stream Nitrogen Concentrations Across Arctic Vegetation Gradients. Global Biogeochemical Cycles, 38(2), e2023GB007840, https://doi.org/10.1029/2023GB007840

This study integrates molecular, environmental, and spatiotemporal data to examine the structure and function of streambed microbial communities across Arctic regions experiencing rapid climate change. Sediment samples were collected from 28 streams spanning four Arctic regions during summer 2021, including 15 Greenlandic streams that were sampled three times throughout the open-water season to capture temporal variation. Microbial community composition was characterized using 16S rRNA gene sequencing, while functional potential for nitrogen cycling was quantified through qPCR targeting six key genes involved in N-fixation (nifH), nitrification (amoA, nxrB), and denitrification (nirS, norB, nosZ). Environmental data included catchment characteristics, streamwater and sediment caractheristics. These molecular and environmental datasets were integrated with spatial and temporal sampling metadata to assess biogeographic and seasonal trends. Together, these data provide a comprehensive framework for understanding how Arctic stream microbial communities and nitrogen cycling functions respond to spatial heterogeneity and seasonal dynamics under ongoing climate change. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.988290"!

http://en.wikipedia.org/wiki/ISO_8601 DATE/TIME http://en.wikipedia.org/wiki/Loss_on_ignition Loss on ignition https://doi.org/10.1029/2023GB007840 Stream Nitrogen Concentrations Across Arctic Vegetation Gradients https://en.wikipedia.org/wiki/Grade_%28slope%29 Slope https://en.wikipedia.org/wiki/Normalized_Difference_Vegetation_Index Normalized Difference Vegetation Index DIF 9.4 2026-02-09 2026-04-02