de.pangaea.dataset987865 und dataset PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact 2026-02-15T13:06:59Z https://doi.pangaea.de/10.1594/PANGAEA.987865 <gco:CharacterString>Relative proportions of sterols in Melosira assemblages, and subsequently isolated unialgal cultures collected in June 2023 during the RV Kronprins Haakon cruise 2023-1 in Fram Strait</gco:CharacterString> 2026-01-07T12:45:39 publication https://doi.org/10.1594/PANGAEA.987865 Leu, Eva
eva.leu@akvaplan.niva.no
https://orcid.org/0000-0002-5328-3396 information author Šupraha, Luka https://orcid.org/0000-0003-2613-945X information author Graeve, Martin
martin.graeve@awi.de
https://orcid.org/0000-0002-2294-1915 information author Fahl, Kirsten
kirsten.fahl@awi.de
https://orcid.org/0000-0001-9317-4656 information author Müller, Anja
anja.mueller@awi.de
author Müller, Juliane
juliane.mueller@awi.de
https://orcid.org/0000-0003-0724-4131 information author Lunden, Jonas
jonas.lunden@awi.de
author Woll, Matthias
Matthias.Woll@awi.de
author Kohlbach, Doreen https://orcid.org/0000-0002-9245-2500 information author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital During the AO2023-1 expedition (1-21 June 2023), Melosira assemblages were collected using a handpump at a sampling site in Fram Strait. In addition to performing physiological measurements and filtering the material for trophic markers (fatty acids, stable isotope ratios of fatty acids, sterols and highly branched isoprenoids (HBIs), living material was brought back to Akvaplan-niva in Oslo and two strains were established as unialgal cultures. The cultures are maintained in artificial seawater-based L1 medium at 4°C and 25 µmol m-2 s-1 light with a 16:8 L:D cycle. Lipids were extracted from freeze-dried samples following the method of Folch et al. (1957), using a dichloromethane/methanol mixture (2:1, v/v) in a sonication bath. Thereafter, lipids were saponified with 20% potassium hydroxide in water/methanol (1:9). Sterols were extracted with hexane, purified by open-column chromatography with SiO2 and eluted with methanol. Sterol fractions were derivatized using N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA). Sterols were analyzed with gas chromatography–mass spectrometry (Agilent 7890B, Agilent Technologies), equipped with a DB-1ms column (30 m length, 0.25 mm internal diameter, 0.25 μm film thickness) and an Agilent 5977A MSD, following Stein et al. (2025). The temperature program ranged from 60°C to 320°C. Sterols were quantified using an internal standard (androstanol). Sterol analysis was conducted to complement datasets on fatty acid proportions and fatty acid stable isotope compositions. Collectively, the datasets enable a detailed characterization of the biochemical composition of different Melosira arctica-dominated algal assemblages from the Arctic Ocean, and a comparison with a unialgal culture of this important species. completed Leu, Eva
eva.leu@akvaplan.niva.no
https://orcid.org/0000-0002-5328-3396 information principalInvestigator Arctic microalgae Melosira Standard cultivation Sterols trophic markers theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright restricted copyright CC-BY-4.0: Creative Commons Attribution 4.0 International (License comes into effect after moratorium ends) textTable und geoscientificInformation -2.1387 -2.1387 78.9397 78.9397 2023-06-18T00:00:00 2023-06-18T00:00:00 0.0 0.0 Event label thematicClassification Station label thematicClassification Type of study thematicClassification Sample code/label thematicClassification DEPTH, water physicalMeasurement m m Depth comment thematicClassification Genus, unique identification thematicClassification Reference in WoRMS Genus, unique identification (Semantic URI) thematicClassification Reference in WoRMS Genus, unique identification (URI) thematicClassification Reference in WoRMS 24-Methylcholesta-5,22E-dien-3beta-ol physicalMeasurement Gas chromatograph-mass spectrometer % % 24-methylcholest-5-en-3beta-ol physicalMeasurement Gas chromatograph-mass spectrometer % % 3beta-Stigmast-5-en-3-ol physicalMeasurement Gas chromatograph-mass spectrometer % % Cholesta-5,24-dien-3beta-ol physicalMeasurement Gas chromatograph-mass spectrometer % % Date/time start, experiment thematicClassification Unialgal cultures cultivation Date/time end, experiment thematicClassification Unialgal cultures cultivation Experimental treatment thematicClassification Unialgal cultures cultivation Treatment: temperature physicalMeasurement Unialgal cultures cultivation °C °C Treatment: salinity thematicClassification Unialgal cultures cultivation Treatment: nitrate physicalMeasurement Unialgal cultures cultivation; as NaNO3 µmol/l µmol/l Treatment: silicate physicalMeasurement Unialgal cultures cultivation; as Na2SiO3 µmol/l µmol/l Treatment: phosphate physicalMeasurement Unialgal cultures cultivation; as K2HPO4 µmol/l µmol/l text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.987865?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: AO2023-1_MA1 * LATITUDE: 78.939700 * LONGITUDE: -2.138700 * DATE/TIME: 2023-06-18T00:00:00 * LOCATION: Fram Strait * CAMPAIGN: AO2023-1 * BASIS: Kronprins Haakon * METHOD/DEVICE: Hand pump 2023-06-18T00:00:00 Related to <gco:CharacterString>Melosira arctica: algal superfood or nutritional dead-end for Arctic grazers?</gco:CharacterString> Kohlbach, Doreen https://orcid.org/0000-0002-9245-2500 information author Kunisch, Erin H
erin.kunisch@ntnu.no
https://orcid.org/0000-0002-9877-4930 information author Graeve, Martin
martin.graeve@awi.de
https://orcid.org/0000-0002-2294-1915 information author Fahl, Kirsten
kirsten.fahl@awi.de
https://orcid.org/0000-0001-9317-4656 information author Müller, Anja
anja.mueller@awi.de
author Müller, Juliane
juliane.mueller@awi.de
https://orcid.org/0000-0003-0724-4131 information author Lunden, Jonas
jonas.lunden@awi.de
author Woll, Matthias
Matthias.Woll@awi.de
author Leu, Eva
eva.leu@akvaplan.niva.no
https://orcid.org/0000-0002-5328-3396 information author documentHardcopy Elementa - Science of the Anthropocene References <gco:CharacterString>A simple method for the isolation and purification of total lipides from animal tissues</gco:CharacterString> 1957-01-01 publication https://doi.org/10.1016/S0021-9258(18)64849-5 Folch, Jordi author Lees, M author Sloane Stanley, G H author documentDigital Journal of Biological Chemistry 226(1) 497-509 References <gco:CharacterString>A 430 kyr record of ice-sheet dynamics and organic-carbon burial in the central Eurasian Arctic Ocean</gco:CharacterString> 2025-01-01 publication https://doi.org/10.1038/s41467-025-59112-7 Stein, Ruediger
ru_st@uni-bremen.de
https://orcid.org/0000-0002-4453-9564 information author Frederichs, Thomas
frederichs@uni-bremen.de
https://orcid.org/0000-0003-0976-0332 information author Fahl, Kirsten
kirsten.fahl@awi.de
https://orcid.org/0000-0001-9317-4656 information author Geibert, Walter
walter.geibert@awi.de
https://orcid.org/0000-0001-8646-2334 information author Matthiessen, Jens
jens.matthiessen@awi.de
https://orcid.org/0000-0002-6952-2494 information author Niessen, Frank
frank.niessen@awi.de
https://orcid.org/0000-0001-6453-0594 information author Vogt, Christoph
cvogt@uni-bremen.de
https://orcid.org/0000-0002-5376-9011 information author Sassenroth, Cynthia
cynthia.sassenroth@phd.unipi.it
author Bazhenova, Evgenia A
evgeniabazhenova@gmail.com
https://orcid.org/0000-0002-8092-702X information author documentDigital Nature Communications 16(1) 3822