Citation:

PANGAEA.986451

Name: Seawater carbonate chemistry and activity, escape response, and muscle physiology of marine threespine stickleback (Gasterosteus aculeatus)
Published: 2025-09-30
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gasterosteus aculeatus; Growth/Morphology; Laboratory experiment; Nekton; North Pacific; Other metabolic rates; Other studied parameter or process; Pelagos; Respiration; Single species; Temperate

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Published: 2025-09-30 • DOI registered: 2025-10-29

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Abstract:

We exposed threespine stickleback (Gasterosteus aculeatus) to pCO2 of ~ 700 µatm (pH 7.9 representing current levels), ~ 1400 µatm (pH 7.6 representing upwelling events) and ~ 3500 µatm (pH 7.3 representing a future predicted scenario for coastal areas) for 2 weeks. We evaluated the baseline activity, interindividual distance as a proxy for group sociality, and escape response. Then, we measured whole-body routine metabolic rate and maximum metabolic rate to determine if changes in locomotory traits might be due to changes in metabolism. We also assessed lactate dehydrogenase (LDH; EC 1.1.1.27), which is a glycolytic enzyme that interconverts pyruvate and NADH to lactate and NAD+ as well as citrate synthase (CS; EC 2.3.3.1), which is a mitochondrial enzyme that catalyzes the first step of TCA cycle to synthesize citrate from acetyl-CoA and oxaloacetate. Additionally, we also measured muscle fiber size and succinate dehydrogenase staining as a measure of muscular metabolism.

This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were downloaded from Figshare (see Source) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2025-09-16.

Keyword(s):

Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gasterosteus aculeatus; Growth/Morphology; Laboratory experiment; Nekton; North Pacific; Other metabolic rates; Other studied parameter or process; Pelagos; Respiration; Single species; Temperate

Related to:

Yoon, Gwangseok R; Khodikian, Elissa; Ren, Gary J; Porteus, Cosima S (2025): Effects of Simulated Ocean Acidification on the Activity, Escape Response, and Muscle Physiology of Marine Threespine Stickleback ( Gasterosteus aculeatus ). Journal of Experimental Zoology Part A-Ecological Genetics and Physiology, jez.70034, https://doi.org/10.1002/jez.70034

Source:

Effects of simulated ocean acidification on the activity, escape response and muscle physiology of marine threespine stickleback (Gasterosteus aculeatus) [dataset]. figshare, https://figshare.com/s/0d8335835005a14691d7

References:

Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010

Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024

Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016

Related code / software:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 48.815300 * Longitude: -125.156400

Date/Time Start: 2022-06-01T00:00:00 * Date/Time End: 2022-06-30T00:00:00

Event(s):

Bamfield_inlet_OA * Latitude: 48.815300 * Longitude: -125.156400 * Date/Time Start: 2022-06-01T00:00:00 * Date/Time End: 2022-06-30T00:00:00 * Method/Device: Experiment (EXP)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Yoon, Gwangseok R
2	Species, unique identification	Species UID		Yoon, Gwangseok R
3	Species, unique identification (URI)	Species UID (URI)		Yoon, Gwangseok R
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Yoon, Gwangseok R
5	Date	Date		Yoon, Gwangseok R
6	Treatment	Treat		Yoon, Gwangseok R
7	Exposure	Exposure		Yoon, Gwangseok R
8	Identification	ID		Yoon, Gwangseok R		Trial
9	Time of day	Time of day		Yoon, Gwangseok R
10	Local Time	Local time		Yoon, Gwangseok R
11	Time in minutes	Time min	min	Yoon, Gwangseok R		Acclimation
12	Time in minutes	Time min	min	Yoon, Gwangseok R		Measurement
13	Identification	ID		Yoon, Gwangseok R		Fish
14	Total distance moved	Tot dist mov	pixel	Yoon, Gwangseok R		baseline activity
15	Average speed	Avg speed	pixel/s	Yoon, Gwangseok R		baseline activity
16	Average interindividual distance	Avg interind dist	pixel	Yoon, Gwangseok R		baseline activity
17	Identification	ID		Yoon, Gwangseok R		Well
18	Sample ID	Sample ID		Yoon, Gwangseok R
19	Citrate synthase activity, per protein mass	CS/prot	µmol/min/mg	Yoon, Gwangseok R
20	Lactate dehydrogenase activity, per protein mass	LDH/prot	µmol/min/mg	Yoon, Gwangseok R
21	Lactate dehydrogenase activity/citrate synthase activity	LDH/CS		Yoon, Gwangseok R
22	Identification	ID		Yoon, Gwangseok R
23	Total distance moved	Tot dist mov	pixel	Yoon, Gwangseok R		during escape responses
24	Average speed	Avg speed	pixel/s	Yoon, Gwangseok R		during escape responses
25	Maximum speed	Max speed	pixel/s	Yoon, Gwangseok R		during escape responses
26	Length, total	TL	mm	Yoon, Gwangseok R
27	Mass	Mass	g	Yoon, Gwangseok R		body
28	Sampling	Sampling		Yoon, Gwangseok R
29	Measurement conducted	Measurement		Yoon, Gwangseok R
30	Group	Group		Yoon, Gwangseok R		Muscle
31	Intensity	Intensity		Yoon, Gwangseok R
32	Comment	Comment		Yoon, Gwangseok R
33	Standardized area	Std area	µm²/g	Yoon, Gwangseok R
34	Identification	ID		Yoon, Gwangseok R		Chamber
35	Mass	Mass	g	Yoon, Gwangseok R
36	Metabolic rate of oxygen	MR O2	mg/kg/h	Yoon, Gwangseok R		Routine
37	Metabolic rate of oxygen, standard error	MR O2 std e	±	Yoon, Gwangseok R
38	Replicates	Repl	#	Yoon, Gwangseok R		Routine metabolic rate
39	Metabolic rate, maximum	MMR	mg/kg/h	Yoon, Gwangseok R
40	Absolute aerobic scope	AAS	mg/kg/h	Yoon, Gwangseok R
41	Comment	Comment		Yoon, Gwangseok R
42	pH, NBS scale	pH NBS		Yoon, Gwangseok R
43	pH, standard deviation	pH std dev	±	Yoon, Gwangseok R
44	Temperature, water	Temp	°C	Yoon, Gwangseok R
45	Temperature, water, standard deviation	Temp std dev	±	Yoon, Gwangseok R
46	Salinity	Sal		Yoon, Gwangseok R
47	Salinity, standard deviation	Sal std dev	±	Yoon, Gwangseok R
48	Alkalinity, total	AT	µmol/kg	Yoon, Gwangseok R
49	Alkalinity, total, standard deviation	AT std dev	±	Yoon, Gwangseok R
50	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yoon, Gwangseok R
51	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yoon, Gwangseok R
52	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	pH, total scale	pHT		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

121843 data points

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