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Zander, A; O'Corcora, Tadhg; Bockelmann, Anna-Christina; Reusch, Thorsten B H; Stevenson, Angela (2025): Zostera marina seedling emergence count in laboratory experiments, Kiel, Germany (2023) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.984655, In: Zander, A et al. (2025): Zostera marina seedling emergence count under varying salinity, temperature, seed colour, sowing time & Arenicola marina bioturbation in field & lab experiments, Kiel, Germany (2023) [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.984654

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Published: 2025-09-11DOI registered: 2025-10-10

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Abstract:
Seed-based seagrass restoration is expanding globally, to speed up the slow natural recolonization success of Zostera marina meadows. This study aimed to find practical guidelines to enhance seed-based restoration efforts in the German Baltic Sea, and other regions. To this end, parallel field and laboratory experiments were conducted, to examine the effects of six biotic and abiotic factors on seedling emergence (germination) over time.
Seeds were collected by snorkelers from two sites (Falckenstein, Kiekut) in Schleswig-Holstein, Germany between July and August 2022.
In the laboratory, 12 aquaria containing six boxes each, were sown with 100 seeds of one of three seed coat colours (grey, dark, light ) from both seed origin sites. Aquaria were subjected to one of four treatments: two temperatures (5 ºC , 10 ºC ) and two salinities (5 psu , 18-20 psu). Seedling counts and aquaria salinity and temperature were monitored five days a week from March 8th, to June 1st 2023.
Keyword(s):
Baltic Sea; bioturbation; phenology; restoration; Salinity; Temperature
Funding:
Bundesministerium für Bildung und Forschung, Bonn (BMBF), grant/award no. 03F0859: SeaStore
Helmholtz Association of German Research Centres (HGF), grant/award no. HI-CAM: Helmholtz-Climate-Initiative
Coverage:
Median Latitude: 54.420555 * Median Longitude: 10.030833 * South-bound Latitude: 54.392778 * West-bound Longitude: 9.871111 * North-bound Latitude: 54.448333 * East-bound Longitude: 10.190556
Date/Time Start: 2023-02-26T00:00:00 * Date/Time End: 2023-06-01T00:00:00
Minimum Elevation: -2.5 m * Maximum Elevation: -2.5 m
Event(s):
Falckenstein_2022 * Latitude Start: 54.392778 * Longitude Start: 10.190556 * Latitude End: 54.392778 * Longitude End: 10.190556 * Date/Time Start: 2022-07-20T00:00:00 * Date/Time End: 2022-08-03T00:00:00 * Elevation Start: -2.5 m * Elevation End: -2.5 m * Method/Device: Sampling by snorkeling (SNORKELING)
Kiekut_2022 * Latitude Start: 54.448333 * Longitude Start: 9.871111 * Latitude End: 54.448333 * Longitude End: 9.871111 * Date/Time Start: 2022-07-20T00:00:00 * Date/Time End: 2022-08-03T00:00:00 * Elevation Start: -2.5 m * Elevation End: -2.5 m * Method/Device: Sampling by snorkeling (SNORKELING)
Zostera_marina_lab_experiment_2023 * Date/Time Start: 2023-02-26T00:00:00 * Date/Time End: 2023-06-01T00:00:00 * Method/Device: Laboratory experiment
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sample code/labelSample labelReusch, Thorsten B HSeed origin location
2DATE/TIMEDate/TimeReusch, Thorsten B HGeocode – count and measurement
3Species, unique identificationSpecies UIDReusch, Thorsten B H
4Sample code/labelSample labelReusch, Thorsten B HBox ID
5Zostera marinaZ. marina#Reusch, Thorsten B Hseedling count [#/100]
6Zostera marinaZ. marina#Reusch, Thorsten B Hasymptotic seedling number
7Zostera marinaZ. marina#/m2Reusch, Thorsten B Hseedling density
8DATE/TIMEDate/TimeReusch, Thorsten B HGeocode – seed sowing
9DATE/TIMEDate/TimeReusch, Thorsten B HGeocode – tank temperature change
10DATE/TIMEDate/TimeReusch, Thorsten B HGeocode – seed sowing adapt
11Experiment dayExp daydayReusch, Thorsten B Hsince sown
12Experiment dayExp daydayReusch, Thorsten B Hsince sown adapt
13Treatment: temperatureT:temp°CReusch, Thorsten B Htank water
14Temperature, waterTemp°CReusch, Thorsten B H
15TreatmentTreatReusch, Thorsten B HSalinity, tank water [psu]
16SalinitySalReusch, Thorsten B H
17TreatmentTreatReusch, Thorsten B HSeed coat colour
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
43560 data points

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