Calogiuri, Tullia; Hagens, Mathilde; van Groenigen, Jan Willem; Wichern, Florian; Poetra, Reinaldy P; Rieder, Lukas; Janssens, Ivan A; Hartmann, Jens; Neubeck, Anna; Niron, Harun; Singh, Abhijeet; Vlaeminck, Siegfried E; Vicca, Sara; Vidal, Alix (2025): Influence of live and dead earthworms on carbon stabilization and sequestration through mineral weathering - Organo-mineral mixture and leachate [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.984355, In: Calogiuri, T et al. (2025): Influence of live and dead earthworms on carbon stabilization and sequestration through mineral weathering [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.984356
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Published: 2025-09-11 • DOI registered: 2025-09-11
Abstract:
The dataset consists of a list of treatments and relative analyses done to determine different pathways through which live and dead earthworms can influence organic carbon stabilisation and inorganic carbon sequestration through mineral weathering. Data was obtained from a column-experiment of the duration of 120 days, with a mid-term sampling at 60 days to observe changes over time, done between October 2023 and February 2024. The column-experiment was carried out in an incubator at 20 °C, where columns where filled with a mixture of rock powder and 13C labelled plant residues, and earthworms were introduced either alive or dead. The dataset relates to the list of treatments and parameters measured to determine the effect of earthworms, either alive or dead, on carbon dynamics measured at 60 and 120 days. These include measurements such as 13C analyzed in dissolved inorganic carbon, mineral associated organic carbon, microbial analyses, and a series of weathering indicators, as alkalinity and major cations.
Keyword(s):
Supplement to:
Calogiuri, Tullia; Hagens, Mathilde; van Groenigen, Jan Willem; Wichern, Florian; Poetra, Reinaldy P; Rieder, Lukas; Janssens, Ivan A; Hartmann, Jens; Neubeck, Anna; Niron, Harun; Singh, Abhijeet; Vlaeminck, Siegfried E; Vicca, Sara; Vidal, Alix (accepted): Alive and dead earthworms capture carbon during mineral weathering through different pathways. Communications Earth & Environment
Related to:
Calogiuri, Tullia; Hagens, Mathilde; van Groenigen, Jan Willem; Wichern, Florian; Poetra, Reinaldy P; Rieder, Lukas; Janssens, Ivan A; Hartmann, Jens; Neubeck, Anna; Niron, Harun; Singh, Abhijeet; Vlaeminck, Siegfried E; Vicca, Sara; Vidal, Alix (2025): Influence of live and dead earthworms on carbon stabilization and sequestration through mineral weathering - Gas emissions [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.984354
References:
Cerli, Chiara; Celi, Luisella Roberta; Kalbitz, Karsten; Guggenberger, Georg; Kaiser, Klaus-Felix (2012): Separation of light and heavy organic matter fractions in soil — Testing for proper density cut-off and dispersion level. Geoderma, 170, 403-416, https://doi.org/10.1016/j.geoderma.2011.10.009
Dalby, Paul R; Baker, Geoff H; Smith, Sally E (1996): "Filter paper method" to remove soil from earthworm intestines and to standardise the water content of earthworm tissue. Soil Biology and Biochemistry, 28(4-5), 685-687, https://doi.org/10.1016/0038-0717(95)00157-3
Plaza, César; Giannetta, Beatrice; Benavente, Iria; Vischetti, Costantino; Zaccone, Claudio (2019): Density-based fractionation of soil organic matter: effects of heavy liquid and heavy fraction washing. Scientific Reports, 9(1), 10146, https://doi.org/10.1038/s41598-019-46577-y
Project(s):
Funding:
Coverage:
Latitude: 51.980768 * Longitude: 5.659193
Date/Time Start: 2023-10-11T10:30:00 * Date/Time End: 2023-10-11T10:30:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Calogiuri, Tullia | Column experiment | ||
| 2 | Date/time start, experiment | Date/time start exp | Calogiuri, Tullia | Date of the start of the experiment | ||
| 3 | Date/time end, experiment | Date/time end exp | Calogiuri, Tullia | Date of the end of the experiment | ||
| 4 | Experimental treatment | Exp treat | Calogiuri, Tullia | Description of the type of treatment | ||
| 5 | Experimental treatment | Exp treat | Calogiuri, Tullia | Code of each treatment | ||
| 6 | Column number | Column No | Calogiuri, Tullia | Number assigned to each column | ||
| 7 | Experiment duration | Exp duration | days | Calogiuri, Tullia | Sampling time at which columns were sampled (either after 60 or 120 days) | |
| 8 | Treatment: temperature | T:temp | °C | Calogiuri, Tullia | Incubator | Temperature during the experiment |
| 9 | Treatment: coarse basanite | T:coarse basanite | g | Calogiuri, Tullia | Weighed | Mass of basanite with coarse grain size added to the columns |
| 10 | Treatment: fine basanite | T:fine basanite | g | Calogiuri, Tullia | Weighed | Mass of basanite with fine grain size added to the columns |
| 11 | Treatment: pea straw | T:pea straw | g | Calogiuri, Tullia | Weighed | Mass of pea straw added to the columns |
| 12 | Species, unique identification | Species UID | Calogiuri, Tullia | Species of earthworms added to the columns | ||
| 13 | Species, unique identification (URI) | Species UID (URI) | Calogiuri, Tullia | Species of earthworms added to the columns | ||
| 14 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Calogiuri, Tullia | Species of earthworms added to the columns | ||
| 15 | Treatment: number of individuals, total | T:Ind No tot | # | Calogiuri, Tullia | Counting, visual | Number of earthworms added to the columns |
| 16 | Treatment: abundance, wet mass | T:abundance wm | g | Calogiuri, Tullia | Starved and weighed according to Dalby et al. (1996) | Total wet mass of initially added earthworms; after starvation according to Dalby et al. (1996) |
| 17 | Number of individuals, survived | Ind No survived | # | Calogiuri, Tullia | Counting, visual | Number of earthworms survived |
| 18 | Sample mass | Samp m | g | Calogiuri, Tullia | Weighed dry mass of sample used for organic matter density fractionation according to Cerli et al. (2012) and Plaza et al. (2019) | Dry mass of the sample used for organic matter density fractionation according to Cerli et al. (2012) and Plaza et al. (2019) |
| 19 | Organic matter, particulate, free | POM free | g | Calogiuri, Tullia | Weighed dry mass of fraction; after organic matter density fractionation according to Cerli et al. (2012) and Plaza et al. (2019) | Dry mass of the free particulate organic matter fraction |
| 20 | Organic matter, particulate, occluded | POM occluded | g | Calogiuri, Tullia | Weighed dry mass of fraction; after organic matter density fractionation according to Cerli et al. (2012) and Plaza et al. (2019) | Dry mass of the occluded particulate organic matter fraction |
| 21 | Organic matter, mineral-associated | MAOM | g | Calogiuri, Tullia | Weighed dry mass of fraction; after organic matter density fractionation according to Cerli et al. (2012) and Plaza et al. (2019) | Dry mass of the mineral associated organic fraction |
| 22 | Carbon/organo-mineral mixture | C/org-min mix | mg/g | Calogiuri, Tullia | Elemental analyzer CHN, LECO, TruSpec | Amount of carbon (C) in the bulk organo-mineral mixture |
| 23 | Carbon/free particulate organic matter | C/POM free | mg/g | Calogiuri, Tullia | Micro CN elemental analyzer, Thermo Fisher Scientific, FlashSmart | Amount of carbon (C) in free particulate organic matter |
| 24 | Carbon/occluded particulate organic matter | C/POM occluded | mg/g | Calogiuri, Tullia | Micro CN elemental analyzer, Thermo Fisher Scientific, FlashSmart | Amount of carbon (C) in occluded particulate organic matter |
| 25 | Carbon/mineral-associated organic matter | C/MAOM | mg/g | Calogiuri, Tullia | Elemental analyzer CHN, LECO, TruSpec | Amount of carbon (C) in mineral associated organic matter |
| 26 | Bacteria, gram-positive, per soil dry mass | Bact gram +/soil dm | pmol/kg | Calogiuri, Tullia | Gas chromatography-mass spectrometer (GC-MS), Agilent Technologies | Abundance of gram-positive bacteria measured in the organo-mineral mixture at 120 days |
| 27 | Bacteria, gram-negative, per soil dry mass | Bact gram -/soil dm | pmol/kg | Calogiuri, Tullia | Gas chromatography-mass spectrometer (GC-MS), Agilent Technologies | Abundance of gram-negative bacteria measured in the organo-mineral mixture at 120 days |
| 28 | Actinomycetes, per soil dry mass | Actinomycetes/soil dm | pmol/kg | Calogiuri, Tullia | Gas chromatography-mass spectrometer (GC-MS), Agilent Technologies | Abundance of actinomycetes measured in the organo-mineral mixture at 120 days |
| 29 | Eukaryotes, per soil dry mass | Euka/soil dm | pmol/kg | Calogiuri, Tullia | Gas chromatography-mass spectrometer (GC-MS), Agilent Technologies | Abundance of eukaryote measured in the organo-mineral mixture at 120 days |
| 30 | Fungi, arbuscular mycorrhizal, per soil dry mass | AMF/soil dm | pmol/kg | Calogiuri, Tullia | Gas chromatography-mass spectrometer (GC-MS), Agilent Technologies | Abundance of arbuscular mychorrizal fungi measured in the organo-mineral mixture at 120 days |
| 31 | Fungi, per soil dry mass | Fungi/soil dm | pmol/kg | Calogiuri, Tullia | Gas chromatography-mass spectrometer (GC-MS), Agilent Technologies | Abundance of fungi measured in the organo-mineral mixture at 120 days |
| 32 | Leachate, tare | Leachate tare | g | Calogiuri, Tullia | Weighed | Mass of the container (= tare weight) to collect the leachate |
| 33 | Leachate, gross | Leachate gross | g | Calogiuri, Tullia | Weighed | Mass of the container (= tare weight) and the leachate collected (= net weight) = gross wight |
| 34 | Leachate, net | Leachate net | g | Calogiuri, Tullia | Calculated | Total leachate mass collected (= net weight) |
| 35 | Alkalinity, total | AT | mmol(eq)/l | Calogiuri, Tullia | pH meter; after titration with 0.02 M HCl until reached pH = 4.5 | Alkalinity measured in the leachate |
| 36 | Carbon, inorganic, dissolved | DIC | mg/l | Calogiuri, Tullia | Segmented Flow Analyzer (SFA), Skalar Analytical B.V., San++ Automated Wet Chemistry Analyzer | Dissolved organic carbon measured in the leachate |
| 37 | Carbon, total dissolved | TDC | mg/l | Calogiuri, Tullia | Segmented Flow Analyzer (SFA), Skalar Analytical B.V., San++ Automated Wet Chemistry Analyzer | Dissolved total carbon measured in the leachate |
| 38 | Carbon, organic, dissolved | DOC | mg/l | Calogiuri, Tullia | Calculated | Dissolved organic carbon in the leachate; as the difference between dissolved total carbon (TDC) and dissolved inorganic carbon (DIC) |
| 39 | Conductivity | Cond | mS/cm | Calogiuri, Tullia | Multi-parameter portable meter, WTW, MultiLine® 2630 IDS | |
| 40 | pH | pH | Calogiuri, Tullia | Multi-parameter portable meter, WTW, MultiLine® 2630 IDS | pH measured in the leachate | |
| 41 | Calcium | Ca | mg/l | Calogiuri, Tullia | ICP-OES, Thermo Scientific, iCAP 6500 dual view | Calcium concentration measured in the leachate |
| 42 | Iron | Fe | mg/l | Calogiuri, Tullia | ICP-OES, Thermo Scientific, iCAP 6500 dual view | Iron concentration measured in the leachate |
| 43 | Potassium | K | mg/l | Calogiuri, Tullia | ICP-OES, Thermo Scientific, iCAP 6500 dual view | Potassium concentration measured in the leachate |
| 44 | Magnesium | Mg | mg/l | Calogiuri, Tullia | ICP-OES, Thermo Scientific, iCAP 6500 dual view | Magnesium concentration measured in the leachate |
| 45 | Sodium | Na | mg/l | Calogiuri, Tullia | ICP-OES, Thermo Scientific, iCAP 6500 dual view | Sodium concentration measured in the leachate |
| 46 | Phosphorus | P | mg/l | Calogiuri, Tullia | ICP-OES, Thermo Scientific, iCAP 6500 dual view | Phopshorus concentration measured in the leachate |
| 47 | Silicon | Si | mg/l | Calogiuri, Tullia | ICP-OES, Thermo Scientific, iCAP 6500 dual view | Silicon concentration measured in the leachate |
| 48 | Atom percent, 13C, dissolved total carbon | At perc 13C TDC | % | Calogiuri, Tullia | Elemental Analyzer, Elementar Analysensysteme GmbH, Langenselbold, Germany, Elementar vario MICRO cube; coupled with Isotope Ratio Mass Spectrometer, Sercon, Europa 20-20 | 13C isotopic signature measured in total dissolved carbon (TDC) |
| 49 | Atom percent, 13C, dissolved organic carbon | At perc 13C DOC | % | Calogiuri, Tullia | Elemental Analyzer, Elementar Analysensysteme GmbH, Langenselbold, Germany, Elementar vario MICRO cube; coupled with Isotope Ratio Mass Spectrometer, Sercon, Europa 20-20 | 13C isotopic signature measured in dissolved organic carbon (DOC) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1636 data points