Papadimitraki, Maria; Wünsch, Urban; Glud, Ronnie N (2025): Excitation-emission matrices produced by custom-built high-pressure cuvette system [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.984327
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Published: 2025-10-27 • DOI registered: 2025-10-27
Abstract:
A custom high-pressure cuvette system was developed, capable of operating at pressures up to 110 MPa to investigate pressure-dependent effects on enzymatic activity and biogeochemical element cycling in the deep ocean. This system features three sapphire windows for real-time absorption and fluorescence measurements, as well as temperature control. This dataset contains the maximum-normalized fluorescence measurements obtained using this system, for assessing the enzymatic activity of extracellular chitinase produced by a piezo-sensitive Vibrio anguillarum (PF430-3, originally isolated in Chile) strain. Specifically, the dataset includes excitation-emission matrices (EEMs) for the substrate 4-MUF-NAG, the fluorophore 4-MUF and the combined signal from 4-MUF-NAG in 30% medium along with cleaved 4-MUF in the presence of chitinase enzymes.
Keyword(s):
Supplement to:
Papadimitraki, Maria; Wünsch, Urban; Balmonte, John Paul; Middelboe, Mathias; Stedmon, Colin A; Attard, Karl Michael; Glud, Ronnie N (in review): An ultraviolet-visible spectroscopy system for studying real-time pressure effects on enzyme activity. Limnology and Oceanography-Methods
Related to:
Papadimitraki, Maria; Wünsch, Urban; Glud, Ronnie N (2025): Hydrolysis rates of extracellular chitinase produced by piezo-philic and piezo-sensitive marine bacteria under high hydrostatic pressure [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.984324
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Papadimitraki, Maria | |||
| 2 | Date/time start, experiment | Date/time start exp | Papadimitraki, Maria | |||
| 3 | Date/time end, experiment | Date/time end exp | Papadimitraki, Maria | |||
| 4 | Species, unique identification | Species UID | Papadimitraki, Maria | |||
| 5 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Papadimitraki, Maria | |||
| 6 | Species, unique identification (URI) | Species UID (URI) | Papadimitraki, Maria | |||
| 7 | Substrate type | Substrate | Papadimitraki, Maria | 4-MUF-NAG Sigma #69585 dissolved in DMSO, fluorophore 4-MUF Sigma #M1381 dissolved in DMSO or combined signal of 4-MUF-NAG dissolved in 30 % medium and cleaved fluorophore 4-MUF in presence of enzymes | ||
| 8 | Substrate, concentration | Substrate conc | µmol/l | Papadimitraki, Maria | 4-MUF-NAG Sigma #69585 dissolved in DMSO [300 μmol/L], 4-MUF Sigma #M1381 dissolved in DMSO [4 μmol/L] | |
| 9 | Treatment: pressure | T:pressure | MPa | Papadimitraki, Maria | Pressure conditions during bacterial growth | |
| 10 | Wavelength, emission | WL emission | nm | Papadimitraki, Maria | Emission wavelengths | |
| 11 | Wavelength, excitation | WL excitation | nm | Papadimitraki, Maria | Excitation wavelengths | |
| 12 | Fluorescence, substrate, normalized | F substrate norm | Papadimitraki, Maria | The data has been corrected for inner filter effects and the intensities are scaled to the maximum intensity in each EEM. Raman and Rayleigh scatter have been removed and replaced with an interpolation |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
61295 data points
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