Citation:

PANGAEA.984222

Name: CFU counts of Vibrio spp. on seaweeds and water column at 16°C and 20°C
Published: 2025-08-11
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Laboratory experiment; mesocosm experiment

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Published: 2025-08-11 • DOI registered: 2025-08-11

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Abstract:

We investigated the impact of elevated water temperatures on the abundances of pathogenic Vibrio species associated with seaweeds and coastal waters. Three seaweed species - Fucus serratus, Palmaria palmata, and Ulva spp.- were collected from Plymouth Sound, United Kingdom, and used in a mesocosm experiment conducted at Plymouth Marine Laboratory in June 2024. The samples were exposed to two temperature treatments (16 °C and 20 °C) for seven days each to simulate warming ocean conditions. After the seven-day treatments, microbial suspensions (referred to as microbial soups) were prepared from each seaweed replicate and corresponding water column sample. These suspensions were filtered and incubated on ChromAgar Vibrio chromogenic media plates for 72 hours to selectively identify and differentiate Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio vulnificus/cholerae. Colony-forming units (CFUs) were counted for each replicate, providing quantitative data on the response of these Vibrio species to elevated temperatures.

Keyword(s):

Laboratory experiment; mesocosm experiment

Supplement to:

Wilson, Sidney; Saha, Mahasweta (2025): Warmer Oceans Will Increase Abundance of Human Pathogens on Seaweeds. Phycology, 5(3), 38, https://doi.org/10.3390/phycology5030038

Coverage:

Latitude: 50.364067 * Longitude: -4.145968

Date/Time Start: 2024-06-03T00:00:00 * Date/Time End: 2024-06-03T00:00:00

Event(s):

PlymouthSound_20240603 * Latitude: 50.364067 * Longitude: -4.145968 * Date/Time: 2024-06-03T00:00:00 * Location: United Kingdom * Method/Device: Sampling by hand (HAND) * Comment: Seaweed and water column samples collected for mesocosm experiments

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device
1	Sample type	Samp type		Wilson, Sidney
2	Type of study	Study type		Wilson, Sidney
3	Replicate	Repl		Wilson, Sidney
4	Treatment: temperature	T:temp	°C	Wilson, Sidney
5	Vibrio alginolyticus, abundance in colony forming units	V. alginolyticus CFU	CFU/cm²	Wilson, Sidney	Counted
6	Vibrio parahaemolyticus, abundance in colony forming units	V. parahaemolyticus CFU	CFU/cm²	Wilson, Sidney	Counted
7	Vibrio vulnificus and Vibrio cholerae, abundance in colony forming units	V. vulnificus/V. cholerae CFU	CFU/cm²	Wilson, Sidney	Counted
8	Vibrio alginolyticus, abundance in colony forming units	V. alginolyticus CFU	CFU/ml	Wilson, Sidney	Counted
9	Vibrio parahaemolyticus, abundance in colony forming units	V. parahaemolyticus CFU	CFU/ml	Wilson, Sidney	Counted
10	Vibrio vulnificus and Vibrio cholerae, abundance in colony forming units	V. vulnificus/V. cholerae CFU	CFU/ml	Wilson, Sidney	Counted

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

420 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Samp type	2 Study type	3 Repl	4 T:temp [°C]	5 V. alginolyticus CFU [CFU/cm²]	6 V. parahaemolyticus CFU [CFU/cm²]	7 V. vulnificus/V. cholerae CFU [CFU/cm²]	8 V. alginolyticus CFU [CFU/ml]	9 V. parahaemolyticus CFU [CFU/ml]	10 V. vulnificus/V. cholerae CFU [CFU/ml]
Fucus serratus	Laboratory mesocosm experiment	1	16	93	13	13
Fucus serratus	Laboratory mesocosm experiment	2	16	20	4	3
Fucus serratus	Laboratory mesocosm experiment	3	16	45	14	8
Fucus serratus	Laboratory mesocosm experiment	4	16	19	2	3
Fucus serratus	Laboratory mesocosm experiment	5	16	110	33	20
Fucus serratus water column	Laboratory mesocosm experiment	1	16				221	66	83
Fucus serratus water column	Laboratory mesocosm experiment	2	16				229	43	98
Fucus serratus water column	Laboratory mesocosm experiment	3	16				209	35	58
Fucus serratus water column	Laboratory mesocosm experiment	4	16				224	51	69
Fucus serratus water column	Laboratory mesocosm experiment	5	16				166	73	67
Palmaria palmata	Laboratory mesocosm experiment	1	16	77	32	21
Palmaria palmata	Laboratory mesocosm experiment	2	16	175	25	38
Palmaria palmata	Laboratory mesocosm experiment	3	16	48	20	19
Palmaria palmata	Laboratory mesocosm experiment	4	16	79	18	20
Palmaria palmata	Laboratory mesocosm experiment	5	16	74	11	17
Palmaria palmata water column	Laboratory mesocosm experiment	1	16				281	71	78
Palmaria palmata water column	Laboratory mesocosm experiment	2	16				258	67	88
Palmaria palmata water column	Laboratory mesocosm experiment	3	16				205	31	83
Palmaria palmata water column	Laboratory mesocosm experiment	4	16				240	62	114
Palmaria palmata water column	Laboratory mesocosm experiment	5	16				221	44	96
Ulva spp.	Laboratory mesocosm experiment	1	16	12	3	6
Ulva spp.	Laboratory mesocosm experiment	2	16	72	15	19
Ulva spp.	Laboratory mesocosm experiment	3	16	128	9	18
Ulva spp.	Laboratory mesocosm experiment	4	16	56	13	9
Ulva spp.	Laboratory mesocosm experiment	5	16	96	13	24
Ulva spp. water column	Laboratory mesocosm experiment	1	16				173	107	182
Ulva spp. water column	Laboratory mesocosm experiment	2	16				181	73	139
Ulva spp. water column	Laboratory mesocosm experiment	3	16				140	70	135
Ulva spp. water column	Laboratory mesocosm experiment	4	16				253	105	198
Ulva spp. water column	Laboratory mesocosm experiment	5	16				198	44	94
Fucus serratus	Laboratory mesocosm experiment	1	20	117	12	31
Fucus serratus	Laboratory mesocosm experiment	2	20	121	16	23
Fucus serratus	Laboratory mesocosm experiment	3	20	166	47	58
Fucus serratus	Laboratory mesocosm experiment	4	20	140	27	40
Fucus serratus	Laboratory mesocosm experiment	5	20	197	24	94
Fucus serratus water column	Laboratory mesocosm experiment	1	20				79	10	29
Fucus serratus water column	Laboratory mesocosm experiment	2	20				165	35	60
Fucus serratus water column	Laboratory mesocosm experiment	3	20				72	10	42
Fucus serratus water column	Laboratory mesocosm experiment	4	20				39	15	14
Fucus serratus water column	Laboratory mesocosm experiment	5	20				57	19	14
Palmaria palmata	Laboratory mesocosm experiment	1	20	198	78	136
Palmaria palmata	Laboratory mesocosm experiment	2	20	155	27	65
Palmaria palmata	Laboratory mesocosm experiment	3	20	185	81	92
Palmaria palmata	Laboratory mesocosm experiment	4	20	132	39	86
Palmaria palmata	Laboratory mesocosm experiment	5	20	155	58	113
Palmaria palmata water column	Laboratory mesocosm experiment	1	20				162	62	94
Palmaria palmata water column	Laboratory mesocosm experiment	2	20				110	14	28
Palmaria palmata water column	Laboratory mesocosm experiment	3	20				68	6	16
Palmaria palmata water column	Laboratory mesocosm experiment	4	20				28	8	14
Palmaria palmata water column	Laboratory mesocosm experiment	5	20				92	58	58
Ulva spp.	Laboratory mesocosm experiment	1	20	83	20	22
Ulva spp.	Laboratory mesocosm experiment	2	20	47	13	10
Ulva spp.	Laboratory mesocosm experiment	3	20	87	25	29
Ulva spp.	Laboratory mesocosm experiment	4	20	32	13	21
Ulva spp.	Laboratory mesocosm experiment	5	20	85	21	12
Ulva spp. water column	Laboratory mesocosm experiment	1	20				102	18	28
Ulva spp. water column	Laboratory mesocosm experiment	2	20				145	21	42
Ulva spp. water column	Laboratory mesocosm experiment	3	20				101	16	39
Ulva spp. water column	Laboratory mesocosm experiment	4	20				102	7	32
Ulva spp. water column	Laboratory mesocosm experiment	5	20				148	30	56