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Bracher, Astrid; Xi, Hongyan; Wiegmann, Sonja (2025): Phytoplankton pigment and phytoplankton group chlorophyll-a concentrations during POLARSTERN cruise PS136 from North Sea to Fram Strait in May to June 2023 from HPLC analysis of water samples [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.983243

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Published: 2025-11-18DOI registered: 2025-11-18

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Abstract:
Phytoplankton pigments were determined in the water surface (from 0 to 11 m depth) of a transect from the North Sea to East Greenland Sea and back during RV Polarstern expedition PS136 from 23 May to 17 June 2023. Water samples were collected from CTD Niskin bottles at five to six different depths from the upper 100 m at CTD stations and from underway sampling. Between 0.4 to 3.5 L of each seawater sample was filtered through Whatman GF/F filters. The sample filters were then shock‐frozen in liquid N2 and kept at −80 °C until analysis. High Pressure Liquid Chromatography (HPLC) was performed to quantify various phytoplankton pigments (see Table 1 in Taylor et al. 2011) following the method of Barlow et al. (1997) that was adjusted to our temperature‐controlled instruments as detailed in Alvarez et al. (2022). Data were used to analyse the Fram Strait phytoplankton community composition dynamics in Bracher et al. (2025).
Keyword(s):
Fram Strait; North Sea; phytoplankton functional types; phytoplankton pigments
Supplement to:
Bracher, Astrid; Xi, Hongyan; von Appen, Wilken-Jon; Mehdipour, Ehsan; Metfies, Katja; McPherson, Rebecca; Zeising, Moritz; Nöthig, Eva-Maria (2025): Resolving the interannual variability of phytoplankton community composition in Fram Strait using ship-based high-frequency spectrophotometric measurements from 2015 to 2024. https://doi.org/10.1016/j.dsr2.2025.105568, Deep Sea Research Part II: Topical Studies in Oceanography, https://www.sciencedirect.com/science/article/pii/S0967064525001171
References:
Álvarez, Eva; Losa, Svetlana N; Bracher, Astrid; Thoms, Silke; Völker, Christoph (2022): Phytoplankton Light Absorption Impacted by Photoprotective Carotenoids in a Global Ocean Spectrally-resolved Biogeochemistry Model. Journal of Advances in Modeling Earth Systems, https://doi.org/10.1029/2022MS003126
Barlow, Raymond G; Cummings, Denise G; Gibb, S W (1997): Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC. Marine Ecology Progress Series, 161, 303-307, https://doi.org/10.3354/meps161303
Taylor, Bettina B; Torrecilla, Elena; Bernhardt, Anja; Taylor, Marc H; Peeken, Ilka; Röttgers, Rüdiger; Piera, Jaume; Bracher, Astrid (2011): Bio-optical provinces in the eastern Atlantic Ocean and their biogeographical relevance. Biogeosciences, 8(12), 3609-3629, https://doi.org/10.5194/bg-8-3609-2011
Funding:
German Research Foundation (DFG), grant/award no. 268020496: TRR 172: ArctiC Amplification: Climate Relevant Atmospheric and SurfaCe Processes, and Feedback Mechanisms
Coverage:
Median Latitude: 75.032853 * Median Longitude: 3.567038 * South-bound Latitude: 56.961715 * West-bound Longitude: -5.424210 * North-bound Latitude: 79.763966 * East-bound Longitude: 11.077680
Date/Time Start: 2023-05-23T15:02:00 * Date/Time End: 2023-06-17T10:18:00
Minimum DEPTH, water: m * Maximum DEPTH, water: 11 m
Event(s):
PS136_0_Underway-54 * Latitude Start: 56.961715 * Longitude Start: 5.522825 * Latitude End: 75.901434 * Longitude End: 7.908806 * Date/Time Start: 2023-05-23T15:02:00 * Date/Time End: 2023-06-17T10:18:00 * Campaign: PS136 (HAUSGARTEN; FRAM) * Basis: Polarstern * Method/Device: Underway water sampling (UWS) * Comment: not logged in D-Ship
PS136_4-9 * Latitude: 78.611102 * Longitude: 5.075731 * Date/Time: 2023-05-29T10:08:13 * Elevation: -2326.0 m * O2A Registry URI: https://registry.o2a-data.de/items/1399 * Campaign: PS136 (HAUSGARTEN; FRAM) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO)
PS136_5-1 * Latitude: 79.063895 * Longitude: 4.190881 * Date/Time: 2023-05-30T09:15:27 * Elevation: -2448.0 m * O2A Registry URI: https://registry.o2a-data.de/items/1399 * Campaign: PS136 (HAUSGARTEN; FRAM) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventBracher, Astrid
2Sample IDSample IDBracher, Astrid
3DEPTH, waterDepth watermBracher, AstridGeocode
4DATE/TIMEDate/TimeBracher, AstridGeocode
5LATITUDELatitudeBracher, AstridGeocode
6LONGITUDELongitudeBracher, AstridGeocode
7Chlorophyll c1Chl c1µg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
8Chlorophyll c2Chl c2µg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
9Chlorophyll c3Chl c3µg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
10PeridininPeridµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
11Pheophorbide aPhide aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
1219-ButanoyloxyfucoxanthinBut-fucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
13FucoxanthinFucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
14NeoxanthinNeoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
1519-HexanoyloxyfucoxanthinHex-fucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
16PrasinoxanthinPrasµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
17ViolaxanthinViolaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
18DiadinoxanthinDiadinoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
19DinoxanthinDinoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
20AlloxanthinAlloµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
21DiatoxanthinDiatoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
22ZeaxanthinZeaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
23Monovinyl chlorophyll bMV chl bµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
24Monovinyl chlorophyll aMV chl aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
25alpha-Carotenea-Carµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
26beta-Caroteneb-Carµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
27gamma-Caroteneg-Carµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
2819'-Hexanoyloxy-4-ketofucoxanthin19Hex-4-kfucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
29CrocoxanthinCrocoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
30Chlorophyll a, DiatomsChl a Diatomsµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
31Chlorophyll a, DinoflagellataChl a Dinoflagellataµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
32Chlorophyll a, Green AlgaeChl a GAµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
33Chlorophyll a, HaptophytaChl a Haptophytaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
34Chlorophyll a, ProkaryotesChl a Prokaryotesµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
35Chlorophyll a, CryptophytaChl a Cryptoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
36Chlorophyll a, ChrysophytaChl a Chrysoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
4216 data points

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