Keuter, Sabine; Frada, Miguel J; Koplovitz, Gil; Ben Yehouda, Mayaan (2025): Nutrient limitation bioassays using natural populations of Gephyrocapsa huxleyi from the Gulf of Aqaba in contrasting seasons [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.983184
Always quote citation above when using data! You can download the citation in several formats below.
Published: 2025-06-27 • DOI registered: 2025-07-26
Abstract:
To disentangle the effect of macronutrient availability on cell-size in native populations of coccolithophores we surveyed the seasonality of the prevalent coccolithophores in the northern Red Sea and the Eastern Mediterranean and performed a series of incubations assays. For bioassays in stratified season and during winter mixing water samples were taken from 2 m depth off the pier of the IUI in Eilat (29.501735 N, 34.917801 E) and incubated at in situ temperature for 48 hours. 1 µM NaNO3 or/and 0.0625 µM of Na2 b-glycerophosphate were added to test for nutrient limitations in the Gulf of Aqaba. Scanning electron microscopy was used for coccolithophore enumeration and eukaryotic microalgae cells were identified and counted based on cell size scatter signals and red (chlorophyll) fluorescence using flow cytometry.
Supplement to:
Keuter, Sabine; Koplovitz, Gil; Ben Yehouda, Mayaan; Klaiman, Sivan; Frada, Miguel J (in prep.): Phosphate limitation drives the seasonal increase in coccolithophore cell size in marine ecosystems.
Related to:
Keuter, Sabine; Frada, Miguel J; Koplovitz, Gil; Klaiman, Sivan: Measurements of coccospheres and coccoliths of the species Gephyrocapsa huxleyi and G. ericsonii in natural populations in the Gulf of Aqaba and the Eastern Mediterranean Sea [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.983004
Coverage:
Latitude: 29.501735 * Longitude: 34.917801
Date/Time Start: 2021-07-06T00:00:00 * Date/Time End: 2022-07-25T00:00:00
Minimum DEPTH, water: 2 m * Maximum DEPTH, water: 2 m
Event(s):
IUI_pier_nutrients * Latitude: 29.501735 * Longitude: 34.917801 * Date/Time Start: 2021-07-06T00:00:00 * Date/Time End: 2022-07-27T00:00:00 * Elevation: -5.0 m * Location: Gulf of Aqaba * Method/Device: Water sample (WS) * Comment: Sample water was taken off the pier of the IUI in Eilat, Israel, with a Niskinbottle from 2 m depth by Gil Koplovitz and Mayaan Ben Yehuda.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Keuter, Sabine | sampling station: IUI pier (5 m bottom depth), Gulf of Aqaba, 29.501735 N, 34.917801 E | ||
| 2 | Sample code/label | Sample label | Keuter, Sabine | month and year when experiment was conducted, Juli and August is stratified season, November and January is mixing/winter seasons | ||
| 3 | DATE/TIME | Date/Time | Keuter, Sabine | Geocode | ||
| 4 | DEPTH, water | Depth water | m | Keuter, Sabine | Geocode – sampling depth | |
| 5 | Treatment | Treat | Keuter, Sabine | Fertilization experiment (FERT) | bioassay using natural populations and addition of 1 µM nitrate (plus NO3), 0.0625 µM phosphate (plus PO4), both (plus NO3 & PO4). time zero was taken twice. | |
| 6 | Incubation duration | Inc dur | h | Keuter, Sabine | incubation time until sample was taken | |
| 7 | Gephyrocapsa huxleyi | G. huxleyi | #/ml | Keuter, Sabine | Scanning electron microscopy (SEM) | calculated cell concentration |
| 8 | Counts | Counts | # | Keuter, Sabine | Scanning electron microscopy (SEM) | number of cells counted for calculation of cell concentration |
| 9 | Volume | Vol | ml | Keuter, Sabine | volume analysed with SEM for calculation of cell concentration | |
| 10 | Cell density | Cells | #/ml | Keuter, Sabine | Flow cytometry | pico and nano photoautotrophic eukaryotes cells were identified based on cell size scatter signals and red (chlorophyll) fluorescence (excitation: 488 nm; emission: 663-737) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
472 data points