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Bracher, Astrid; Xi, Hongyan; Hohe, Christian; Wiegmann, Sonja; Peeken, Ilka (2025): Phytoplankton pigment and phytoplankton group chlorophyll-a concentrations during POLARSTERN cruise PS126 from North Sea to Fram Strait in May to June 2021 from HPLC analysis of water samples [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.982954

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Published: 2025-11-18DOI registered: 2025-11-18

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Abstract:
Phytoplankton pigments were determined in the water surface (from 0 to 11 m depth) of a transect from the North Sea to Fram Strait and back during RV Polarstern expedition PS126 from 24 May to 25 June 2021. Water samples were collected from CTD Niskin bottles at five to six different depths from the upper 100 m at CTD stations and from underway sampling. Between 0.4 to 3.5 L of each seawater sample was filtered through Whatman GF/F filters. The sample filters were then shock‐frozen in liquid N2 and kept at−80 °C until analysis. High Pressure Liquid Chromatography (HPLC) was performed to quantify various phytoplankton pigments (see Table 1 in Taylor et al. 2011) following the method of Barlow et al. (1997) that was adjusted to our temperature‐controlled instruments as detailed in Alvarez et al. (2022).
Keyword(s):
HPLC analysis; North Sea; phytoplankton groups; phytoplankton pigments
Supplement to:
Bracher, Astrid; Xi, Hongyan; von Appen, Wilken-Jon; Mehdipour, Ehsan; Metfies, Katja; McPherson, Rebecca; Zeising, Moritz; Nöthig, Eva-Maria (2025): Resolving the interannual variability of phytoplankton community composition in Fram Strait using ship-based high-frequency spectrophotometric measurements from 2015 to 2024. https://doi.org/10.1016/j.dsr2.2025.105568, Deep Sea Research Part II: Topical Studies in Oceanography, https://www.sciencedirect.com/science/article/pii/S0967064525001171
References:
Álvarez, Eva; Losa, Svetlana N; Bracher, Astrid; Thoms, Silke; Völker, Christoph (2022): Phytoplankton Light Absorption Impacted by Photoprotective Carotenoids in a Global Ocean Spectrally-resolved Biogeochemistry Model. Journal of Advances in Modeling Earth Systems, https://doi.org/10.1029/2022MS003126
Barlow, Raymond G; Cummings, Denise G; Gibb, S W (1997): Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC. Marine Ecology Progress Series, 161, 303-307, https://doi.org/10.3354/meps161303
Taylor, Bettina B; Torrecilla, Elena; Bernhardt, Anja; Taylor, Marc H; Peeken, Ilka; Röttgers, Rüdiger; Piera, Jaume; Bracher, Astrid (2011): Bio-optical provinces in the eastern Atlantic Ocean and their biogeographical relevance. Biogeosciences, 8(12), 3609-3629, https://doi.org/10.5194/bg-8-3609-2011
Funding:
German Research Foundation (DFG), grant/award no. 268020496: TRR 172: ArctiC Amplification: Climate Relevant Atmospheric and SurfaCe Processes, and Feedback Mechanisms
Coverage:
Median Latitude: 74.104814 * Median Longitude: 3.788363 * South-bound Latitude: 54.511730 * West-bound Longitude: -5.536280 * North-bound Latitude: 79.945080 * East-bound Longitude: 11.087620
Date/Time Start: 2021-05-24T17:01:40 * Date/Time End: 2021-06-25T16:59:30
Minimum DEPTH, water: m * Maximum DEPTH, water: 11 m
Event(s):
PS126_0_Underway-26 * Latitude: 53.566995 * Longitude: 8.555145 * Date/Time: 2021-05-24T09:28:00 * Elevation: -257.0 m * O2A Registry URI: https://registry.o2a-data.de/items/3220 * Location: North Sea * Campaign: PS126 (GPF 20-1_021) * Basis: Polarstern * Method/Device: Underway water sampling (UWS)
PS126_2-11 * Latitude: 78.608841 * Longitude: 5.064484 * Date/Time: 2021-05-31T14:33:27 * Elevation: -2339.2 m * O2A Registry URI: https://hdl.handle.net/10013/sensor.ebd40c0a-11ea-427a-bb89-4b3dd1b5c3b1 * Location: North Greenland Sea * Campaign: PS126 (GPF 20-1_021) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO)
PS126_3-3 * Latitude: 79.067108 * Longitude: 4.478674 * Date/Time: 2021-06-01T12:06:50 * Elevation: -2282.7 m * O2A Registry URI: https://hdl.handle.net/10013/sensor.ebd40c0a-11ea-427a-bb89-4b3dd1b5c3b1 * Location: North Greenland Sea * Campaign: PS126 (GPF 20-1_021) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventBracher, Astrid
2Sample IDSample IDBracher, Astrid
3DEPTH, waterDepth watermBracher, AstridGeocode
4DATE/TIMEDate/TimeBracher, AstridGeocode
5LATITUDELatitudeBracher, AstridGeocode
6LONGITUDELongitudeBracher, AstridGeocode
7Chlorophyll c1Chl c1µg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
8Chlorophyll c3Chl c3µg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
9Chlorophyll c2Chl c2µg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
10PeridininPeridµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
11Pheophorbide aPhide aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
12Pyropheophorbide aPyrophide aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
1319-ButanoyloxyfucoxanthinBut-fucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
14FucoxanthinFucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
15NeoxanthinNeoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
1619-HexanoyloxyfucoxanthinHex-fucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
17AphanizophyllAphanizoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
18PrasinoxanthinPrasµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
19ViolaxanthinViolaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
20AstaxanthinAstaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
21DiadinoxanthinDiadinoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
22DinoxanthinDinoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
23AntheraxanthinAntheraµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
24AlloxanthinAlloµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
25MyxoxanthophyllMyxoxµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
26DiatoxanthinDiatoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
27ZeaxanthinZeaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
28LuteinLutµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
29Gyroxanthin diesterGyr diesterµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
30Divinyl chlorophyll bDV chl bµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
31Chlorophyll bChl bµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
32EchinenoneEchinµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
33Divinyl chlorophyll aDV chl aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
34Chlorophyll aChl aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
35Pheophytin bPhytin bµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
36Pheophytin aPhytin aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
37alpha-Carotenea-Carµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
38beta-Caroteneb-Carµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
39Pheophytin aPhytin aµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
4019'-Hexanoyloxy-4-ketofucoxanthin19Hex-4-kfucoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
41CrocoxanthinCrocoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
42gamma-Caroteneg-Carµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
43Chlorophyll a, DiatomsChl a Diatomsµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
44Chlorophyll a, DinoflagellataChl a Dinoflagellataµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
45Chlorophyll a, Green AlgaeChl a GAµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
46Chlorophyll a, HaptophytaChl a Haptophytaµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
47Chlorophyll a, ProkaryotesChl a Prokaryotesµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
48Chlorophyll a, CryptophytaChl a Cryptoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
49Chlorophyll a, ChrysophytaChl a Chrysoµg/lBracher, AstridHigh Performance Liquid Chromatography (HPLC)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
7700 data points

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