Maurice Lamontagne Institute, Fisheries and Oceans Canada
Normalised phospholipidome profile of and metadata associated with the abdomen muscle of female Northern shrimp Pandalus borealis collected in the wild from four geographic origins in the Northwest Atlantic
Data Publisher for Earth & Environmental Science
The dataset contains information on the phospholipidome profile of the abdomen muscle of female Northern shrimp Pandalus borealis collected in the wild from four geographic origins in the Northwest Atlantic: St. Lawrence Estuary (SLE; 48° 35' N, 68° 35' W, May 2018; Event "SLE_Pandalus_2018"), Eastern Scotian Shelf (ESS; 45° 23' N, 61° 04' W, February 2019; Event "ESS_Pandalus_2019"), Esquiman Channel (EC; 50° 44' N, 57°29' W, July 2019; Event "EC_Pandalus_2019") and Northeast Newfoundland Coast (NNC; 50° 18' N, 54° 16' W, November 2019, Event "NNC_Pandalus_2019"). Female shrimp were exposed for 30 days under laboratory conditions to several ocean global change scenarios, with three temperatures (low temperature of 2 °C, intermediate temperature of 6 °C, and elevated temperature of 10 °C) and pH (current pH 7.75 and low pH 7.40). This experiment aimed to determine the changes in the phospholipidome profiles of shrimp from different origins in response to different scenarios of future ocean global changes combining Ocean Warming (OW) and Ocean Acidification (OA) and low dissolved oxygen (DO). Phospholipid species were quantified in abdomen muscle of female shrimp using high-resolution HILIC-MS/MS. Several datasets are provided, containing (i) the raw data expressed as peak area, (ii) the peak area data recalibrated to the internal standards for each class of phospholipid, and (iii) the final normalised data following transformation using ordered quantile normalisation, along with (iv) the metadata associated with abdomen muscle samples. A total of 269 phospholipids were identified and quantified from 260 female shrimp, with 10 shrimp per treatment exposed sampled from two tanks per treatment (5 shrimp in each). Phospholipid peak areas determined using Xcalibur data system v3.3 (Thermo Fisher Scientific, USA). Shrimp that molted/died during respirometry experiments were excluded from the analysis. Shrimp that were exposed to low DO were removed from the dataset prior to data transformation and downstream statistical analysis, but the raw data is provided.
Center for Environmental and Marine Studies
Merit Scholarship for Foreign Students (PBEEE) program
Scientific Employment Stimulus researcher contract
Centro de Estudos do Ambiente e do Mar (CESAM)
Natural Sciences and Engineering Research Council
Research Support for New Academics (FRQNT)
Species, unique identification (Semantic URI)
Low temperature (2 °C); Intermediate temperature (6 °C); Elevated temperature (10 °C)
High dioxygen (100 %) saturation; Low dioxygen (35 %) saturation
Modified Bligh and Dyer protocol, HPLC-MS analysis
Wet weight in grams (g) of the abdomen muscle sample. Approx. 0.1 g (100 mg)
Weight in grams (g) of the empty vial, weighed prior to inserting the lipid extract
Weight in grams (g) of the vial with (w) the lipid extract.
Total lipid weight in microgram (µg) per milligram (mg) of sample dry weight
High-resolution LC-MS/MS using liquid chromatograph, Dionex, Ultimate 3000 Dionex; coupled to a mass spectrometer, Thermo Fisher Scientific, Q-Exactive hybrid quadrupole
Acclimatization; adaptation; crustacean; global change; lipidomics; multipopulations; multi-stressors; Pandalus borealis; stress
Treatment - Temperature and pH scenario to which the individuals were exposed 2C - Low temperature (2 °C) and current pH (7.75) 2A - Low temperature (2 °C) and low pH (7.40) 6C - Intermediate temperature (6 °C) and current pH (7.75) 6A - Intermediate temperature (6 °C) and low pH (7.40) 10C - Elevated temperature (10 °C) and current pH (7.75) 10A - Elevated temperature (10 °C) and low pH (7.40) 2HC - Low temperature (2 °C) and current pH (7.75) and 35 % dissolved Oxygen - Excluded from the statistical analysis. 10HA - Elevated temperature (10 °C) and low pH (7.40) and 35 % dissolved Oxygen - Excluded from the statistical analysis.