Citation:

PANGAEA.980734 (DOI registration in progress)

Name: Physiological data of Pacific oyster Crassostrea (Magallana) gigas after exposure to intermittent hypoxia and the combination with (fluctuating) elevated temperature
Published: 2025-04-09
License: https://creativecommons.org/licenses/by/4.0/
Keywords: bioenergetics; crassotrea gigas; ecophysiology; energy reserves; fluctuation; hypoxia; intertidal; invasive species; Laboratory experiment; Magallana gigas; metabolism; metabolome; metabolomics; oxidaitive stress; oxidative damage; Oxygen; Oyster; Temperature; tidal cycle; Waddensea

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Published: 2025-04-09

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Abstract:

Organisms in intertidal zones experience fluctuations in environmental stressors such as hypoxia and temperature. These stressors and their fluctuations often appear in combination. Combination of stressors can have different effects compared to single stressors. In this study, we investigate the physiological effects of intermittent hypoxia in combination with different temperature regimes on the Pacific Oyster Crassostrea (Magallana) gigas. The oysters were exposed to hypoxic cycles (12h hypoxia by emersion/12h submersion) at normal (15°C), elevated (30°C) or fluctuating (15°C submersion/30°C emersion) temperature for 10 days. After the last submersion phase, the gills and digestive gland were sampled. We measured markers for bioenergetics and redox-balance in the gills and digestive gland using colorimetric methods as well as a set of metabolites (predominantly amino acids, osmolytes, anaerobic end products and energetic metabolites) in the gills using LC-MS/MS. Oysters kept submerged for up to 10 days were used as controls.

Keyword(s):

bioenergetics; crassotrea gigas; ecophysiology; energy reserves; fluctuation; hypoxia; intertidal; invasive species; Laboratory experiment; Magallana gigas; metabolism; metabolome; metabolomics; oxidaitive stress; oxidative damage; Oxygen; Oyster; Temperature; tidal cycle; Waddensea

Related to:

Bruhns, Torben; Timm, Stefan; Feußner, Nina; Engelhaupt, Sonja; Labrenz, Matthias; Wegner, K Mathias; Sokolova, Inna M (2023): Combined effects of temperature and emersion-immersion cycles on metabolism and bioenergetics of the Pacific oyster Crassostrea (Magallana) gigas. Marine Environmental Research, 192, 106231, https://doi.org/10.1016/j.marenvres.2023.106231

Funding:

German Research Foundation (DFG), grant/award no. 430581461 : CRASSOBIOM: The role of host-microbiome interactions in physiological performance of the Pacific oyster Crassostrea gigas in extreme habitats

German Research Foundation (DFG), grant/award no. GZ: INST 264/125- 1FUGG: Hochschul-Bau-Förderungsgesetz (HBFG)

Coverage:

Latitude: 55.029060 * Longitude: 8.436970

Date/Time Start: 2020-06-17T00:00:00 * Date/Time End: 2020-06-17T00:00:00

Event(s):

C.Gigas_collection * Latitude: 55.029060 * Longitude: 8.436970 * Date/Time: 2020-06-17T00:00:00 * Location: Königshafen, Sylt, Germany * Comment: Collection was conduction in an area ~150m around the coordinates

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experiment	Exp		Bruhns, Torben
2	Type of study	Study type		Bruhns, Torben
3	Laboratory	Lab		Bruhns, Torben
4	Species, unique identification	Species UID		Bruhns, Torben
5	Origin	Origin		Bruhns, Torben		Specimen
6	Date/time end, experiment	Date/time end exp		Bruhns, Torben
7	Treatment: temperature	T:temp	°C	Bruhns, Torben		Water
8	Treatment: temperature	T:temp	°C	Bruhns, Torben		Air
9	Treatment: air exposure	T:air exp	h/day	Bruhns, Torben
10	Treatment: salinity	T:sal		Bruhns, Torben
11	Mass	Mass	g	Bruhns, Torben
12	Length	l	mm	Bruhns, Torben	Caliper
13	Width	w	mm	Bruhns, Torben	Caliper
14	Sample ID	Sample ID		Bruhns, Torben
15	Specimen identification	Spec ID		Bruhns, Torben
16	Tank number	Tank No		Bruhns, Torben
17	Experimental treatment	Exp treat		Bruhns, Torben
18	Tissue type	Tissue type		Bruhns, Torben
19	Sampling date/time, experiment	Date/time sampling exp		Bruhns, Torben
20	Experiment duration	Exp duration	days	Bruhns, Torben		Exposure
21	Carbohydrates, per fresh mass	CHO fm	mg/g	Bruhns, Torben	Multi-user microplate reader, Molecular devices, SpectraMax iD3
22	Electron transport system activity of oyxgen	ETS	µmol/g/min	Bruhns, Torben	Multi-user microplate reader, Molecular devices, SpectraMax iD3
23	Proteins per fresh mass	Prot	mg/g	Bruhns, Torben	Multi-user microplate reader, Molecular devices, SpectraMax iD3
24	Lipid peroxidation, per fresh mass	LPO/fm	nmol/g	Bruhns, Torben	Multi-user microplate reader, Molecular devices, SpectraMax iD3
25	Protein carbonyl, per protein mass	Prot carbo/prot	nmol/mg	Bruhns, Torben	Multi-user microplate reader, Molecular devices, SpectraMax iD3
26	Antioxidant capacity, in Trolox Equivalents, per fresh mass	TEAC/fm	µmol/g	Bruhns, Torben	Multi-user microplate reader, Molecular devices, SpectraMax iD3
27	Cystine, per fresh mass	Cystine/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
28	Asparagine, per fresh mass	Asparagine/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
29	Aspartate, per fresh mass	Aspartate/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
30	Serine, per fresh mass	Ser/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
31	Alanine, per fresh mass	Ala/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
32	Glycine, per fresh mass	Gly/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
33	Glutamine, per fresh mass	Gln/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
34	Threonine, per fresh mass	Thr/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
35	Methionine sulfoxide, per fresh mass	Met sulfoxide/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
36	Glutamate, per fresh mass	Glutamate/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
37	Proline, per fresh mass	Pro/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
38	Lysine, per fresh mass	Lys/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
39	Histidine, per fresh mass	His/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
40	Adenosine monophosphate, per fresh mass	AMP/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
41	Arginine, per fresh mass	Arg/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
42	Valine, per fresh mass	Val/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
43	Methionine, per fresh mass	Met/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
44	Tyrosine, per fresh mass	Tyr/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
45	Isoleucine, per fresh mass	Ile/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
46	Leucine, per fresh mass	Leu/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
47	Phenylalanine, per fresh mass	Phe/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
48	Tryptophan, per fresh mass	Trp/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
49	Malate, per fresh mass	Malate/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
50	Citrate, per fresh mass	Citrate/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050	Including Isocitrate
51	Succinate, per fresh mass	Succinate/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
52	Taurine, per fresh mass	Tau/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
53	Lactate, per fresh mass	Lactate/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
54	Aconitate, per fresh mass	Aconitate/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
55	gamma-Aminobutyric acid, per fresh mass	g-ABA/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
56	Carnitine, per fresh mass	Carnitine/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
57	Citrulline, per fresh mass	Cir/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
58	Ornithine, per fresh mass	Orn/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
59	Glutathione, per fresh mass	GSH/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050
60	Hydroxyproline, per fresh mass	Hyp/fm	ng/mg	Timm, Stefan	High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4064 data points

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