Hellige, Inga; Akeerath Mundanatt, Aman; Fett, Theresa-Marie; Wölfelschneider, Mirco; Prugger, Jennifer; Geuer, Jana K; Reents, Svenja (2025): Total carbohydrates quantified in sediment cores from coastal vegetated ecosystems [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.979910 (DOI registration in progress)
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Published: 2025-04-28
Abstract:
50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. The total carbohydrate content was assessed using the phenol-sulfuric acid assay (Dubois et al., 1956). Briefly, 100 µL of resuspended samples or extracts were mixed with 100 µL of 5% phenol solution, followed by the addition of 500 µL of concentrated sulfuric acid. The reaction mixture was incubated at room temperature for 10 minutes, then further incubated at 30°C for 20 minutes. Absorbance at 490 nm was measured using a Spectramax Id3 plate reader (Molecular Devices) and quantified against a glucose standard curve.
Keyword(s):
Supplement to:
Akeerath Mundanatt, Aman; Massing, Jana Chiara; Hehemann, Jan-Hendrik; Hellige, Inga (in prep.): Roots of coastal plants stabilize carbon fixed by marine algae.
References:
DuBois, Michel; Gilles, K A; Hamilton, J K; Rebers, P A; Smith, Fred (1956): Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28(3), 350-356, https://doi.org/10.1021/ac60111a017
Project(s):
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03F0896E: DAM CDRmare - sea4soCiety, University of Bremen
Coverage:
Median Latitude: 32.004965 * Median Longitude: 13.845943 * South-bound Latitude: 4.658409 * West-bound Longitude: -75.682996 * North-bound Latitude: 54.692271 * East-bound Longitude: 103.439555
Date/Time Start: 2021-11-08T00:00:00 * Date/Time End: 2022-10-11T00:00:00
Minimum DEPTH, sediment/rock: 0.007 m * Maximum DEPTH, sediment/rock: 0.485 m
Event(s):
GER_HAH_SG_P4_SC * Latitude: 54.612684 * Longitude: 8.823788 * Date/Time: 2021-11-09T00:00:00 * Campaign: s4s_GER-Land_2021_NorthSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
GER_HAH_SG_P6_SC * Latitude: 54.612663 * Longitude: 8.823431 * Date/Time: 2021-11-09T00:00:00 * Campaign: s4s_GER-Land_2021_NorthSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
GER_HAH_SG_P8_SC * Latitude: 54.612680 * Longitude: 8.822986 * Date/Time: 2021-11-09T00:00:00 * Campaign: s4s_GER-Land_2021_NorthSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Hellige, Inga | Universal label | ||
| 2 | LONGITUDE | Longitude | Hellige, Inga | Geocode | ||
| 3 | LATITUDE | Latitude | Hellige, Inga | Geocode | ||
| 4 | DATE/TIME | Date/Time | Hellige, Inga | Geocode | ||
| 5 | Country | Country | Hellige, Inga | GER - Germany, COL - Colombia, MYS - Malaysia | ||
| 6 | Ocean and sea region | OS region | Hellige, Inga | Place | ||
| 7 | Location | Location | Hellige, Inga | HAH - Hamburger Hallig, HEI - Heiligenhafen, MET - Mettgrund, NES - Nessmersiel, SCH - Schleimünde, WEN - Wendtorf, LAN - Langkawi, PAK - Paka, SET - Setiu | ||
| 8 | Ecosystem | Ecosystem | Hellige, Inga | SG - seagrass, SM - saltmarsh, UV - unvegetated area, MG - mangrove | ||
| 9 | Identification | ID | Hellige, Inga | Point | ||
| 10 | Layer number | Layer | Hellige, Inga | Depth layer | ||
| 11 | Sample code/label | Sample label | Hellige, Inga | |||
| 12 | DEPTH, sediment/rock | Depth sed | m | Hellige, Inga | Geocode – mean | |
| 13 | Depth, sediment/rock, top/minimum | Depth sed top | m | Hellige, Inga | ||
| 14 | Depth, sediment/rock, bottom/maximum | Depth sed bot | m | Hellige, Inga | ||
| 15 | Carbohydrates, total, per unit sediment dry mass | CHO/sed dm | mg/g | Hellige, Inga | Phenol-sulfuric acid assay according to Dubois et al. (1956) | Total |
| 16 | Comment | Comment | Hellige, Inga | |||
| 17 | Layer description | Layer | Hellige, Inga |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2844 data points
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