Citation:

PANGAEA.977812

Name: Seawater carbonate chemistry and energy metabolism in the hydrocoral Millepora alcicornis
Published: 2025-03-04
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Millepora alcicornis; Other metabolic rates; Single species; South Atlantic; Tropical

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Published: 2025-03-04 • DOI registered: 2025-04-02

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Abstract:

A mesocosm experiment was conducted to investigate whether three levels of decreasing seawater pH could alter the activity of key enzymes involved in the energy metabolism in Millepora alcicornis. Hydrocorals were acclimated to marine mesocosm conditions for 20 days and then exposed to different seawater pH levels [ambient pH (8.1) and experimental pH (7.8, 7.5 and 7.2)] for 16 and 30 days. Endpoints analyzed included the activity of key enzymes involved in the regulation of the glycolytic pathway (hexokinase and pyruvate kinase), aerobic energy production via the Krebs cycle (citrate synthase) and anaerobic energy production via lactate formation (lactate dehydrogenase).

This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were extracted from tables in the related paper (see Related to) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2025-02-27.

Keyword(s):

Animalia; Benthic animals; Benthos; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Millepora alcicornis; Other metabolic rates; Single species; South Atlantic; Tropical

Related to:

Jesulich, Andrea Carlina; Lauer, Mariana Machado; de Barros Marangoni, Laura Fernandes; Marques, Joseane Aparecida; Zebral, Yuri Dornelles; Pereira, Cristiano Macedo; Duarte, Gustavo Adolpho Santos; Pires, Debora de Oliveira; Castro, Clovis Barreira e; Calderon, Emiliano Nicolas; Bianchini, A (2025): Effect of seawater acidification on energy metabolism in the hydrocoral Millepora alcicornis : inhibition of citrate synthase activity indicates disruption in aerobic pathways. Environmental Science: Advances, 4(3), 447-455, https://doi.org/10.1039/D4VA00083H

References:

Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010

Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024

Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016

Related code / software:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -16.410300 * Longitude: -38.983900

Event(s):

Porto_Seguro * Latitude: -16.410300 * Longitude: -38.983900

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device
1	Type of study	Study type		Bianchini, A
2	Species, unique identification	Species UID		Bianchini, A
3	Species, unique identification (URI)	Species UID (URI)		Bianchini, A
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Bianchini, A
5	Time in days	Time d	days	Bianchini, A
6	Treatment: pH	T:pH		Bianchini, A
7	Replicate	Repl		Bianchini, A
8	Hexokinase activity, per protein mass	HK act/prot	U/mg	Bianchini, A
9	Hexokinase activity, standard deviation	HK act std dev	±	Bianchini, A
10	Pyruvate kinase activity, per protein mass	PK act	U/g	Bianchini, A
11	Pyruvate kinase activity, standard deviation	PK act std dev	±	Bianchini, A
12	Lactate dehydrogenase activity, unit per protein mass	LDH/prot	U/mg	Bianchini, A
13	Lactate dehydrogenase activity, standard deviation	LDH std dev	±	Bianchini, A
14	Citrate synthase activity, unit per protein mass	CS/prot	U/mg	Bianchini, A
15	Citrate synthase activity, standard deviation	CS std dev	±	Bianchini, A
16	pH, free scale	pHF		Bianchini, A
17	pH, standard deviation	pH std dev	±	Bianchini, A
18	Temperature, water	Temp	°C	Bianchini, A
19	Temperature, water, standard deviation	Temp std dev	±	Bianchini, A
20	Salinity	Sal		Bianchini, A
21	Salinity, standard deviation	Sal std dev	±	Bianchini, A
22	Alkalinity, total	AT	mg/l	Bianchini, A
23	Alkalinity, total, standard deviation	AT std dev	±	Bianchini, A
24	Carbon, organic, dissolved	DOC	mg/l	Bianchini, A
25	Carbon, organic, dissolved, standard deviation	DOC std dev	±	Bianchini, A
26	Irradiance	E	µmol photons/m²/s	Bianchini, A
27	Irradiance, standard deviation	E std dev	±	Bianchini, A
28	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	pH, total scale	pHT		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1296 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Time d [days]	6 T:pH	7 Repl	8 HK act/prot [U/mg]	9 HK act std dev [±]	10 PK act [U/g]	11 PK act std dev [±]	12 LDH/prot [U/mg]	13 LDH std dev [±]	14 CS/prot [U/mg]	15 CS std dev [±]	16 pHF	17 pH std dev [±]	18 Temp [°C]	19 Temp std dev [±]	20 Sal	21 Sal std dev [±]	22 AT [mg/l]	23 AT std dev [±]	24 DOC [mg/l]	25 DOC std dev [±]	26 E [µmol photons/m²/s]	27 E std dev [±]	28 CSC flag	29 pHT	30 CO2 [µmol/kg]	31 fCO2water_SST_wet [µatm]	32 pCO2water_SST_wet [µatm]	33 [HCO3]- [µmol/kg]	34 [CO3]2- [µmol/kg]	35 DIC [µmol/kg]	36 AT [µmol/kg]	37 Omega Arg	38 Omega Cal
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	0	8.1	1	0.0213	0.0038	401	21	0.158	0.030	0.0668	0.0077	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	0	8.1	2	0.0209	0.0056	384	22	0.165	0.036	0.0640	0.0094	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	0	8.1	3	0.0229	0.0041	402	15	0.166	0.032	0.0630	0.0103	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	0	8.1	4	0.0208	0.0041	383	16	0.134	0.031	0.0745	0.0089	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	8.1	1	0.0199	0.0022	339	42	0.162	0.027	0.0551	0.0024	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	8.1	2	0.0188	0.0026	352	42	0.160	0.017	0.0531	0.0035	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	8.1	3	0.0165	0.0018	333	36	0.174	0.030	0.0544	0.0042	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	8.1	4	0.0165	0.0020	345	38	0.157	0.026	0.0518	0.0037	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.8	1	0.0228	0.0018	303	22	0.172	0.012	0.0515	0.0028	7.74	0.185	25.00	0.03	35.02	0.41	117.1	1.5	3.56	0.36	296.4	41.5	26	7.63	33.65	1185.22	1189.02	2054.39	95.44	2183.48	2288.55	1.51	2.30
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.8	2	0.0234	0.0015	307	20	0.180	0.005	0.0538	0.0045	7.74	0.185	25.00	0.03	35.02	0.41	117.1	1.5	3.56	0.36	296.4	41.5	26	7.63	33.65	1185.22	1189.02	2054.39	95.44	2183.48	2288.55	1.51	2.30
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.8	3	0.0222	0.0016	281	23	0.182	0.008	0.0556	0.0048	7.74	0.185	25.00	0.03	35.02	0.41	117.1	1.5	3.56	0.36	296.4	41.5	26	7.63	33.65	1185.22	1189.02	2054.39	95.44	2183.48	2288.55	1.51	2.30
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.8	4	0.0219	0.0021	285	22	0.177	0.010	0.0540	0.0033	7.74	0.185	25.00	0.03	35.02	0.41	117.1	1.5	3.56	0.36	296.4	41.5	26	7.63	33.65	1185.22	1189.02	2054.39	95.44	2183.48	2288.55	1.51	2.30
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.5	1	0.0189	0.0012	292	34	0.202	0.025	0.0553	0.0100	7.51	0.160	25.16	0.03	35.13	0.37	115.8	2.6	3.20	0.31	285.5	33.9	26	7.40	58.82	2081.56	2088.22	2119.66	58.38	2236.85	2263.07	0.93	1.40
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.5	2	0.0185	0.0013	269	38	0.189	0.015	0.0565	0.0114	7.51	0.160	25.16	0.03	35.13	0.37	115.8	2.6	3.20	0.31	285.5	33.9	26	7.40	58.82	2081.56	2088.22	2119.66	58.38	2236.85	2263.07	0.93	1.40
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.5	3	0.0191	0.0011	299	24	0.202	0.019	0.0522	0.0110	7.51	0.160	25.16	0.03	35.13	0.37	115.8	2.6	3.20	0.31	285.5	33.9	26	7.40	58.82	2081.56	2088.22	2119.66	58.38	2236.85	2263.07	0.93	1.40
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.5	4	0.0177	0.0009	267	30	0.181	0.024	0.0549	0.0123	7.51	0.160	25.16	0.03	35.13	0.37	115.8	2.6	3.20	0.31	285.5	33.9	26	7.40	58.82	2081.56	2088.22	2119.66	58.38	2236.85	2263.07	0.93	1.40
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.2	1	0.0138	0.0009	260	42	0.172	0.041	0.0633	0.0035	7.20	0.190	25.13	0.04	35.07	0.34	115.9	1.5	3.19	0.34	277.0	39.8	26	7.09	124.35	4395.79	4409.91	2192.74	29.51	2346.60	2265.10	0.47	0.71
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.2	2	0.0133	0.0010	267	48	0.189	0.030	0.0663	0.0016	7.20	0.190	25.13	0.04	35.07	0.34	115.9	1.5	3.19	0.34	277.0	39.8	26	7.09	124.35	4395.79	4409.91	2192.74	29.51	2346.60	2265.10	0.47	0.71
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.2	3	0.0155	0.0008	279	39	0.152	0.036	0.0630	0.0009	7.20	0.190	25.13	0.04	35.07	0.34	115.9	1.5	3.19	0.34	277.0	39.8	26	7.09	124.35	4395.79	4409.91	2192.74	29.51	2346.60	2265.10	0.47	0.71
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	16	7.2	4	0.0142	0.0008	253	35	0.170	0.034	0.0651	0.0022	7.20	0.190	25.13	0.04	35.07	0.34	115.9	1.5	3.19	0.34	277.0	39.8	26	7.09	124.35	4395.79	4409.91	2192.74	29.51	2346.60	2265.10	0.47	0.71
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	8.1	1	0.0140	0.0015	283	38	0.156	0.034	0.0691	0.0088	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	8.1	2	0.0161	0.0014	316	20	0.149	0.036	0.0696	0.0093	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	8.1	3	0.0156	0.0017	321	31	0.139	0.021	0.0699	0.0079	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	8.1	4	0.0136	0.0019	290	41	0.136	0.025	0.0626	0.0094	8.10	0.096	25.19	0.03	35.11	0.39	119.2	1.7	3.43	0.37	285.0	41.3	26	7.99	13.15	465.72	467.21	1844.66	197.78	2055.60	2329.57	3.14	4.76
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.8	1	0.0105	0.0017	264	27	0.149	0.010	0.0284	0.0061	7.74	0.185	25.00	0.03	35.02	0.41	117.1	1.5	3.56	0.36	296.4	41.5	26	7.63	33.65	1185.22	1189.02	2054.39	95.44	2183.48	2288.55	1.51	2.30
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.8	2	0.0121	0.0016	242	30	0.139	0.007	0.0239	0.0042	7.74	0.185	25.00	0.03	35.02	0.41	117.1	1.5	3.56	0.36	296.4	41.5	26	7.63	33.65	1185.22	1189.02	2054.39	95.44	2183.48	2288.55	1.51	2.30
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.8	3	0.0104	0.0019	255	17	0.158	0.009	0.0274	0.0069	7.74	0.185	25.00	0.03	35.02	0.41	117.1	1.5	3.56	0.36	296.4	41.5	26	7.63	33.65	1185.22	1189.02	2054.39	95.44	2183.48	2288.55	1.51	2.30
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.8	4	0.0120	0.0021	247	20	0.145	0.006	0.0249	0.0061	7.74	0.185	25.00	0.03	35.02	0.41	117.1	1.5	3.56	0.36	296.4	41.5	26	7.63	33.65	1185.22	1189.02	2054.39	95.44	2183.48	2288.55	1.51	2.30
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.5	1	0.0125	0.0021	284	52	0.127	0.043	0.0328	0.0136	7.51	0.160	25.16	0.03	35.13	0.37	115.8	2.6	3.20	0.31	285.5	33.9	26	7.40	58.82	2081.56	2088.22	2119.66	58.38	2236.85	2263.07	0.93	1.40
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.5	2	0.0112	0.0018	310	46	0.139	0.053	0.0346	0.0121	7.51	0.160	25.16	0.03	35.13	0.37	115.8	2.6	3.20	0.31	285.5	33.9	26	7.40	58.82	2081.56	2088.22	2119.66	58.38	2236.85	2263.07	0.93	1.40
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.5	3	0.0116	0.0021	279	57	0.113	0.056	0.0349	0.0125	7.51	0.160	25.16	0.03	35.13	0.37	115.8	2.6	3.20	0.31	285.5	33.9	26	7.40	58.82	2081.56	2088.22	2119.66	58.38	2236.85	2263.07	0.93	1.40
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.5	4	0.0111	0.0020	280	58	0.131	0.051	0.0368	0.0127	7.51	0.160	25.16	0.03	35.13	0.37	115.8	2.6	3.20	0.31	285.5	33.9	26	7.40	58.82	2081.56	2088.22	2119.66	58.38	2236.85	2263.07	0.93	1.40
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.2	1	0.0113	0.0018	333	10	0.144	0.008	0.0386	0.0024	7.20	0.190	25.13	0.04	35.07	0.34	115.9	1.5	3.19	0.34	277.0	39.8	26	7.09	124.35	4395.79	4409.91	2192.74	29.51	2346.60	2265.10	0.47	0.71
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.2	2	0.0119	0.0016	355	12	0.130	0.009	0.0395	0.0031	7.20	0.190	25.13	0.04	35.07	0.34	115.9	1.5	3.19	0.34	277.0	39.8	26	7.09	124.35	4395.79	4409.91	2192.74	29.51	2346.60	2265.10	0.47	0.71
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.2	3	0.0130	0.0019	340	13	0.144	0.014	0.0437	0.0060	7.20	0.190	25.13	0.04	35.07	0.34	115.9	1.5	3.19	0.34	277.0	39.8	26	7.09	124.35	4395.79	4409.91	2192.74	29.51	2346.60	2265.10	0.47	0.71
laboratory	Millepora alcicornis	marinespecies.org	urn:lsid:marinespecies.org:taxname:210726	30	7.2	4	0.0117	0.0019	309	11	0.140	0.012	0.0445	0.0039	7.20	0.190	25.13	0.04	35.07	0.34	115.9	1.5	3.19	0.34	277.0	39.8	26	7.09	124.35	4395.79	4409.91	2192.74	29.51	2346.60	2265.10	0.47	0.71