Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Tischer, Jana; Zopfi, Jakob; Lepori, Fabio; Lehmann, Moritz F (2025): Incubation experiments with ¹⁵N-tracers in the water column of the Lake Lugano North Basin, Switzerland [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.977625

Always quote citation above when using data! You can download the citation in several formats below.

Published: 2025-06-16 • DOI registered: 2025-07-15

RIS CitationBibTeX Citation ShareShow MapGoogle Earth

Abstract:
In incubation experiments with ¹⁵N-labelled nitrate, without or with additional sulfide or acetate, we determined rates of denitrification, anammox, and dissimilatory nitrate reduction to ammonium to study nitrogen removal versus nitrogen recycling processes. Water column samples were collected from the meromictic Lake Lugano North Basin, Switzerland, during field campaigns in February 2017, October 2017, and April 2018, using Niskin bottles at depths between 95 and 105 m.
Keyword(s):
15N-tracer; anammox; anoxic hypolimnion; Denitrification; dissimilatory nitrate reduction to ammonium; Lake; meromixis; nitrate; Nitrate isotopes; nitrate reduction; nitrification; Nitrogen; nitrogen cycle; nitrogen isotopes; oxycline; Sulfur; sulfur cycle; water column
Supplement to:
Tischer, Jana; Lehmann, Moritz F; Su, Guangyi; Lepori, Fabio; Zopfi, Jakob (preprint): Microbial nitrogen removal versus recycling in the redox transition zone of a meromictic lake and its coupling to sulfur. https://doi.org/10.1101/2025.01.03.627289
Tischer, Jana; Zopfi, Jakob; Frey, Claudia; Venetz, Jessica; Giglio, Elena; Burgdorfer, Lukas; Rehmann, Oliver; Wankel, Scott D; Lehmann, Moritz F (preprint): Nitrogen and oxygen isotope effects during enzymatic nitrate reduction in vitro and by natural lake water consortia. https://doi.org/10.1101/2025.03.29.645582
Funding:
Swiss National Science Foundation (SNF), grant/award no. 153055: Seasonal Dynamics of fixed N elimination and its coupling with Sulfur and Carbon Cycling in the Redox Transition Zones of Lake Lugano
Coverage:
Latitude: 46.010472 * Longitude: 9.020806
Date/Time Start: 2017-10-17T00:00:00 * Date/Time End: 2018-04-25T00:00:00
Minimum DEPTH, water: 95 m * Maximum DEPTH, water: 105 m
Event(s):
LakeLugano * Latitude: 46.010472 * Longitude: 9.020806 * Date/Time Start: 2015-02-17T10:30:00 * Date/Time End: 2018-04-25T15:00:00 * Location: Switzerland * Method/Device: Multiple investigations (MULT) * Comment: 12 sampling campaigns between 2015 and 2018
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 DATE/TIMEDate/TimeTischer, JanaGeocode – Date of water sampling and start of incubation
2 DEPTH, waterDepth watermTischer, JanaGeocode – Water used for experiment
3 Nitrogen-15, tracer15N tracerµmol/lTischer, JanaTargeted initial concentration of 15[NO3]- tracer
4 Nitrogen-15, tracer, fraction15N tracer fracTischer, JanaCalculated
5 TreatmentTreatTischer, JanaAdditional substrate: none, H2S or NaAc
6 TreatmentTreatTischer, JanaAdditional substrate concentration in µmol/l
7 Nitrogen gas, 30N2, production30N2 prodµmol/l/dayTischer, Jana15N tracer incubations (Wenk et al. 2013)
8 Nitrogen gas, 30N2, production, standard error30N2 prod std e±Tischer, Jana15N tracer incubations (Wenk et al. 2013)
9 Nitrogen gas, 29N2, production29N2 prodµmol/l/dayTischer, Jana15N tracer incubations (Wenk et al. 2013)
10 Nitrogen gas, 29N2, production, standard error29N2 prod std e±Tischer, Jana15N tracer incubations (Wenk et al. 2013)
11 Ammonium-15, production15[NH4]+ prodµmol/l/dayTischer, JanaIRMS, hypobromite method (Robertson et al. 2016)
12 Ammonium-15, production, standard error15[NH4]+ prod std e±Tischer, JanaIRMS, hypobromite method (Robertson et al. 2016)
13 Nitrogen gas, 30N2, production30N2 prod%Tischer, JanaCalculatedRelative contribution of mass 30 dinitrogen gas production with respect to the combined production of mass 29 and mass 30 dinitrogen gas, and 15N-[NH4]+
14 Nitrogen gas, 29N2, production29N2 prod%Tischer, JanaCalculatedRelative contribution of mass 29 dinitrogen gas production with respect to the combined production of mass 29 and mass 30 dinitrogen gas, and 15N-[NH4]+
15 Ammonium-15, production15[NH4]+ prod%Tischer, JanaCalculatedRelative contribution of 15N-[NH4]+ production with respect to the combined production of mass 29 and mass 30 dinitrogen gas, and 15N-[NH4]+
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
111 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Date/Time
(Date of water sampling and st...)		2 
Depth water [m]
(Water used for experiment)		3 
15N tracer [µmol/l]
(Targeted initial concentratio...)		4 
15N tracer frac
(Calculated)		5 
Treat
(Additional substrate: none, H...)		6 
Treat
(Additional substrate concentr...)		7 
30N2 prod [µmol/l/day]
(15N tracer incubations (Wenk ...)		8 
30N2 prod std e [±]
(15N tracer incubations (Wenk ...)		9 
29N2 prod [µmol/l/day]
(15N tracer incubations (Wenk ...)		10 
29N2 prod std e [±]
(15N tracer incubations (Wenk ...)		11 
15[NH4]+ prod [µmol/l/day]
(IRMS, hypobromite method (Rob...)		12 
15[NH4]+ prod std e [±]
(IRMS, hypobromite method (Rob...)		13 
30N2 prod [%]
(Relative contribution of mass...)		14 
29N2 prod [%]
(Relative contribution of mass...)		15 
15[NH4]+ prod [%]
(Relative contribution of 15N-...)
2017-10-1795250.9740.06000.01390.00150.00090.00000.00009820
2017-10-1795250.974NaAc250.02550.00540.00160.00070.00000.00009460
2017-10-1795250.974H2S700.07100.00580.00070.00040.47110.025413087
2018-04-2595250.9250.11300.06530.01200.00470.14240.057542453
2018-04-2595250.925NaAc250.01940.00290.00190.00090.00000.00009190
2018-04-2595250.925H2S300.03910.00170.00010.00001.13920.05993097
2018-04-25105250.9830.02880.00420.01250.00200.00000.000070300
2018-04-25105250.983NaAc250.03170.00310.01070.00200.16130.070716579
2018-04-25105250.983H2S1000.06930.00350.00450.00211.70660.08664096