Lipidomics analysis (TAG) from water bottle samples in and around a cyclonic eddy during METEOR cruise M156, off Mauritania
type: Dataset
creator: Kevin W Becker
creator: Quentin Devresse
creator: Xavier Prieto-Mollar
  type: Person
  family name: Prieto-Mollar
  given name: Xavier
creator: Kai-Uwe Hinrichs
creator: Anja Engel
publisher: PANGAEA
  type: Organization
  disambiguating description: Data Publisher for Earth & Environmental Science
included in data catalog: PANGAEA
  type: DataCatalog
  disambiguating description: Data Publisher for Earth & Environmental Science
date published: 2025-03-28
is part of: https://doi.org/10.1594/PANGAEA.974978
additional type: dataset
description: This dataset includes lipidomics data over the surface mixed layer (0-35 m depth) of eleven stations with five of them inside or in the vicinity of a mesoscale cyclonic eddy that formed off Mauritania along a 900 km zonal corridor between Mauritania and the Cabo Verde islands in the eastern Tropical North Atlantic during the M156 cruise on the RV Meteor from July 3rd to August 1st 2019. Lipid samples were collected onto 0.22 µm Durapore filters (Millipore) and immediately flash-frozen and stored at −80 °C until processing. Extraction was performed using a modified Bligh and Dyer (Bligh and Dyer, 1959; https://doi.org/10.1139/o59-099) method described in Popendorf et al. (2013; https://doi.org/10.1007/s11745-012-3748-0). Lipid extracts were analyzed by HPLC-ESI-MS/MS on a Bruker maXis Ultra-High Resolution quadrupole time-of-flight tandem mass spectrometer (Bruker Daltonik, Bremen, Germany) equipped with an ESI ion source operating in positive mode. The HPLC-ESI-MS method is described in Wörmer et al., 2013 (https://doi.org/10.1016/j.orggeochem.2013.03.004). Lipids were identified by retention time as well as accurate molecular mass and isotope pattern match of proposed sum formulas in full scan mode and MS/MS fragment spectra. Lipid identities are resolved only to the level of lipid species, i.e., the sum of the properties of the substituents at both the sn-1 and sn-2 positions. After identification, quantification of analytes was performed using a series of standard curves, followed by normalization to concentration of an internal standard.
abstract: This dataset includes lipidomics data over the surface mixed layer (0-35 m depth) of eleven stations with five of them inside or in the vicinity of a mesoscale cyclonic eddy that formed off Mauritania along a 900 km zonal corridor between Mauritania and the Cabo Verde islands in the eastern Tropical North Atlantic during the M156 cruise on the RV Meteor from July 3rd to August 1st 2019. Lipid samples were collected onto 0.22 µm Durapore filters (Millipore) and immediately flash-frozen and stored at −80 °C until processing. Extraction was performed using a modified Bligh and Dyer (Bligh and Dyer, 1959; https://doi.org/10.1139/o59-099) method described in Popendorf et al. (2013; https://doi.org/10.1007/s11745-012-3748-0). Lipid extracts were analyzed by HPLC-ESI-MS/MS on a Bruker maXis Ultra-High Resolution quadrupole time-of-flight tandem mass spectrometer (Bruker Daltonik, Bremen, Germany) equipped with an ESI ion source operating in positive mode. The HPLC-ESI-MS method is described in Wörmer et al., 2013 (https://doi.org/10.1016/j.orggeochem.2013.03.004). Lipids were identified by retention time as well as accurate molecular mass and isotope pattern match of proposed sum formulas in full scan mode and MS/MS fragment spectra. Lipid identities are resolved only to the level of lipid species, i.e., the sum of the properties of the substituents at both the sn-1 and sn-2 positions. After identification, quantification of analytes was performed using a series of standard curves, followed by normalization to concentration of an internal standard.
citation: Mixed-layer lipidomes suggest offshore transport of energy-rich and essential lipids by cyclonic eddies
spatial coverage: Place
  geo: GeoShape
    box: 17.9995 -24.333799999999997 21.167 -16.5163
temporal coverage: 2019-07-04T07:58:00/2020-07-27T06:00:00
size: QuantitativeValue
  value: 7634.0
  unit text: data points
variable measured: Event label
variable measured: Station label
variable measured: Sample code/label
variable measured: DATE/TIME
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    has defined term: Time
variable measured: LATITUDE
variable measured: LONGITUDE
variable measured: Sample comment
variable measured: DEPTH, water
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  unit text: m
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    has defined term: water
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variable measured: Water volume, filtered
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  unit text: ml
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    has defined term: water column
variable measured: Triacylglycerols
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keywords: Carbon cycle; Cyclonic eddies; lipidomics; Phytoplankton; upwelling systems
license: https://creativecommons.org/licenses/by/4.0/
conditions of access: unrestricted
is accessible for free: true
language: en
distribution: DataDownload

Kevin W Becker
type: Person
family name: Becker
given name: Kevin W
affiliation: GEOMAR - Helmholtz Centre for Ocean Research Kiel

GEOMAR - Helmholtz Centre for Ocean Research Kiel
type: Organization
alternate name: GEOMAR

Quentin Devresse
type: Person
family name: Devresse
given name: Quentin
affiliation: GEOMAR - Helmholtz Centre for Ocean Research Kiel

Kai-Uwe Hinrichs
type: Person
family name: Hinrichs
given name: Kai-Uwe

Anja Engel
type: Person
family name: Engel
given name: Anja
affiliation: GEOMAR - Helmholtz Centre for Ocean Research Kiel

Mixed-layer lipidomes suggest offshore transport of energy-rich and essential lipids by cyclonic eddies
type: CreativeWork
type: ScholarlyArticle
creator: Kevin W Becker
creator: Quentin Devresse
creator: Xavier Prieto-Mollar
  type: Person
  family name: Prieto-Mollar
  given name: Xavier
creator: Kai-Uwe Hinrichs
creator: Anja Engel
date published: 2025
pagination: 179
is part of: Communications Earth & Environment
  type: Periodical
  type: PublicationVolume
  volume number: 6(1)
additional type: journal article

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water
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