Citation:

PANGAEA.975002

Name: Metabolites in heart tissue measured during heat ramp exposure experiments on the cellular metabolism of the European oyster, Ostrea edulis
Published: 2025-02-05
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bivalve; Europe; European flat oyster; heart; Laboratory experiment; metabolomics; Ocean climate change; temperature ramp

Always quote citation above when using data! You can download the citation in several formats below.

Published: 2025-02-05 • DOI registered: 2025-03-06

RIS Citation BibTeX Citation Text Citation ShareShow Map Google Earth

Abstract:

This dataset contains heart tissue metabolite data measured during heat ramp experiments of the European oyster, Ostrea edulis. We studied organismal and heart tissue cellular responses of the European oyster, Ostrea edulis, challenged by rapid warming (+2°C/h) and gradual warming (+2°C/24h). Specimens were collected as wild adults from the estuary of the Ría de Ferrol (Spain, 43°27.64'N and 8°12.04'W) in October 2020 and March 2021. Specimens were sampled at each temperature and dissected on ice. One dimensional 1H-NMR spectroscopy was carried out on heart tissue in an ultra-shielded vertical 9.4 T NMR spectrometer (Advance III HD 400 WB, Bruker-BioSpin GmbH, Germany) equipped with a triple tuned 1H-13C-15N-probe for 1.7 mm NMR tubes. See manuscript for further methodological details and results interpretation.

Keyword(s):

Bivalve; Europe; European flat oyster; heart; Laboratory experiment; metabolomics; Ocean climate change; temperature ramp

Related to:

Götze, Sandra; Reddin, Carl; Ketelsen, Isabel; Busack, Michael; Lannig, Gisela; Bock, Christian; Pörtner, Hans-Otto (2025): Cardiac performance mirrors the passive thermal tolerance range in the oyster Ostrea edulis. Journal of Experimental Biology, 228(2), JEB249750, https://doi.org/10.1242/jeb.249750

Funding:

German Research Foundation (DFG), grant/award no. 290461607 : BivCRS2 - Time dependence of thermal tolerance in marine bivalves: extant fauna and implications for palaeo-analogues

German Research Foundation (DFG), grant/award no. 424857030 : The role of the thermal niche in past warming worlds (ThermNiche)

Coverage:

Latitude: 43.460670 * Longitude: -8.200670

Date/Time Start: 2020-10-01T00:00:00 * Date/Time End: 2021-03-31T00:00:00

Event(s):

Ostrea-Edulis_Ria-de-Ferrol * Latitude: 43.460670 * Longitude: -8.200670 * Date/Time Start: 2020-10-01T00:00:00 * Date/Time End: 2021-03-31T00:00:00 * Location: Ría de Ferrol estuary * Method/Device: Biological sample (BIOS)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Reddin, Carl
2	Species, unique identification	Species UID		Reddin, Carl
3	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Reddin, Carl
4	Species, unique identification (URI)	Species UID (URI)		Reddin, Carl
5	Sample ID	Sample ID		Reddin, Carl		ID of experimentally sacrificed individual
6	Treatment: temperature	T:temp	°C	Reddin, Carl
7	Ramping mode	Ramping		Reddin, Carl		rapid or gradual rate of warming
8	Homarine	Homarine	mmol/l	Reddin, Carl	1H NMR spectroscopy
9	Taurine	Tau	mmol/l	Reddin, Carl	1H NMR spectroscopy
10	Trimethylamine N-oxide	TMAO	mmol/l	Reddin, Carl	1H NMR spectroscopy
11	Betaine	Betaine	mmol/l	Reddin, Carl	1H NMR spectroscopy
12	beta-Alanine	b-ala	mmol/l	Reddin, Carl	1H NMR spectroscopy
13	Glutamate	Glutamate	mmol/l	Reddin, Carl	1H NMR spectroscopy
14	Lysine	Lys	mmol/l	Reddin, Carl	1H NMR spectroscopy
15	Aspartate	Aspartate	mmol/l	Reddin, Carl	1H NMR spectroscopy
16	Glycine	Gly	mmol/l	Reddin, Carl	1H NMR spectroscopy
17	Maltose	Maltose	mmol/l	Reddin, Carl	1H NMR spectroscopy
18	gamma-Methylhistidine	gamma-Methylhistidine	mmol/l	Reddin, Carl	1H NMR spectroscopy
19	Threonine	Thr	mmol/l	Reddin, Carl	1H NMR spectroscopy
20	Acetate	Acetate	mmol/l	Reddin, Carl	1H NMR spectroscopy
21	Asparagine	Asn	mmol/l	Reddin, Carl	1H NMR spectroscopy
22	Uridine diphosphate N-acetylglucosamine	UDP-GlcNAc	mmol/l	Reddin, Carl	1H NMR spectroscopy
23	Succinate	Succinate	mmol/l	Reddin, Carl	1H NMR spectroscopy
24	Alanine	Ala	mmol/l	Reddin, Carl	1H NMR spectroscopy
25	Tyrosine	Tyr	mmol/l	Reddin, Carl	1H NMR spectroscopy
26	Phenylalanine	Phe	mmol/l	Reddin, Carl	1H NMR spectroscopy
27	Tryptophan	Trp	mmol/l	Reddin, Carl	1H NMR spectroscopy
28	Hypotaurine	Hypotaurine	mmol/l	Reddin, Carl	1H NMR spectroscopy
29	Tauropine	Tauropine	mmol/l	Reddin, Carl	1H NMR spectroscopy
30	Fumarate	Fumarate	mmol/l	Reddin, Carl	1H NMR spectroscopy
31	Adenylates, total	Adenylates	mmol/l	Reddin, Carl	1H NMR spectroscopy
32	O-Phosphocholine	O-Phosphocholine	mmol/l	Reddin, Carl	1H NMR spectroscopy
33	O-Acetylcholine	O-Acetylcholine	mmol/l	Reddin, Carl	1H NMR spectroscopy
34	Choline	Choline	mmol/l	Reddin, Carl	1H NMR spectroscopy
35	Leucine	Leu	mmol/l	Reddin, Carl	1H NMR spectroscopy
36	Valine	Val	mmol/l	Reddin, Carl	1H NMR spectroscopy
37	Isoleucine	Ile	mmol/l	Reddin, Carl	1H NMR spectroscopy

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3185 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (Semantic URI)	4 Species UID (URI)	5 Sample ID	6 T:temp [°C]	7 Ramping	8 Homarine [mmol/l]	9 Tau [mmol/l]	10 TMAO [mmol/l]	11 Betaine [mmol/l]	12 b-ala [mmol/l]	13 Glutamate [mmol/l]	14 Lys [mmol/l]	15 Aspartate [mmol/l]	16 Gly [mmol/l]	17 Maltose [mmol/l]	18 gamma-Methylhistidine [mmol/l]	19 Thr [mmol/l]	20 Acetate [mmol/l]	21 Asn [mmol/l]	22 UDP-GlcNAc [mmol/l]	23 Succinate [mmol/l]	24 Ala [mmol/l]	25 Tyr [mmol/l]	26 Phe [mmol/l]	27 Trp [mmol/l]	28 Hypotaurine [mmol/l]	29 Tauropine [mmol/l]	30 Fumarate [mmol/l]	31 Adenylates [mmol/l]	32 O-Phosphocholine [mmol/l]	33 O-Acetylcholine [mmol/l]	34 Choline [mmol/l]	35 Leu [mmol/l]	36 Val [mmol/l]	37 Ile [mmol/l]
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe160	22	Rapid	397.00	21.37	14.93	14.97	0.55	2.20	0.28	2.84	1.00	0.55	0.12	0.22	0.10	0.11	0.08	0.05	1.08	0.07	0.05	0.04	0.00	0.00	0.07	0.45	0.42	0.06	0.05	0.08	0.09	0.06
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe161	22	Rapid	1218.56	84.86	47.06	64.65	0.55	5.29	1.28	9.90	4.18	1.58	0.25	0.93	0.36	0.52	0.19	0.08	1.84	0.19	0.15	0.09	0.01	0.00	0.05	1.43	1.44	0.39	0.13	0.29	0.25	0.16
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe162	22	Rapid	1586.34	146.60	25.29	112.64	1.07	7.89	1.20	11.59	3.08	0.66	0.27	0.68	0.39	0.52	0.18	0.14	1.90	0.32	0.14	0.09	0.01	0.00	0.14	1.61	1.52	0.50	0.20	0.35	0.33	0.19
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 163	22	Rapid	843.94	92.25	47.42	64.43	1.17	7.14	1.50	9.86	5.10	0.99	0.37	1.11	0.52	0.37	0.26	0.29	3.46	0.21	0.19	0.10	0.01	0.00	0.08	1.41	0.54	0.50	0.74	0.52	0.58	0.33
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 200K	22	Rapid	1235.85	152.37	25.06	95.81	2.99	7.34	0.95	9.59	9.29	2.26	0.39	1.30	0.40	0.52	0.22	0.12	5.76	0.25	0.17	0.09	0.06	0.00	0.05	1.66	1.74	0.41	0.09	0.34	0.51	0.18
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 201k	22	Rapid	751.30	94.38	24.15	66.33	0.76	3.20	1.04	9.48	3.30	1.79	0.21	0.98	0.45	0.22	0.27	0.08	1.24	0.16	0.16	0.14	0.00	0.00	0.04	1.22	2.23	0.36	0.22	0.32	0.25	0.15
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 202k	22	Rapid	672.29	32.17	23.50	20.07	1.22	2.38	0.42	5.43	1.61	1.11	0.29	0.31	0.13	0.13	0.13	0.03	0.78	0.08	0.07	0.05	0.01	0.00	0.03	0.81	0.59	0.15	0.08	0.15	0.08	0.06
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 203k	22	Rapid	975.77	69.33	40.21	50.54	0.81	2.66	0.90	9.13	2.11	0.91	0.16	1.36	0.31	0.29	0.18	0.12	0.85	0.12	0.09	0.05	0.01	0.00	0.02	1.17	1.25	0.31	0.48	0.15	0.19	0.10
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe168	26	Rapid	1588.00	140.34	23.09	96.27	0.96	3.78	1.01	8.08	2.58	0.92	0.40	0.65	0.33	0.51	0.19	0.47	2.83	0.12	0.07	0.02	0.01	0.00	0.12	0.91	0.27	0.39	0.58	0.22	0.25	0.13
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe169	26	Rapid	1011.21	114.84	26.63	79.06	1.21	5.77	1.19	11.18	7.10	2.04	0.34	0.96	0.38	0.38	0.19	0.46	4.00	0.16	0.18	0.20	0.00	0.00	0.13	1.46	2.93	0.54	0.11	0.53	0.45	0.27
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe170	26	Rapid	1531.53	156.46	31.01	104.96	1.56	7.26	0.74	9.39	3.68	0.90	0.27	0.50	0.29	0.19	0.16	0.21	1.85	0.19	0.08	0.05	0.01	0.00	0.10	1.58	1.17	0.33	0.04	0.24	0.19	0.12
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe171	26	Rapid	930.45	85.46	43.22	55.20	0.91	6.92	1.02	8.16	4.24	0.95	0.17	0.73	0.21	0.30	0.22	0.17	2.61	0.22	0.14	0.10	0.01	0.00	0.11	1.30	1.11	0.31	0.06	0.29	0.34	0.18
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 204k	26	Rapid	396.20	18.18	10.20	19.51	0.83	1.51	0.39	2.47	2.00	1.03	0.13	0.39	0.11	0.26	0.15	0.07	1.16	0.00	0.06	0.00	0.01	0.00	0.02	0.66	0.35	0.11	0.10	0.12	0.10	0.04
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 205k	26	Rapid	1393.13	137.33	27.62	92.60	3.16	8.24	1.63	11.54	5.52	1.92	0.53	1.49	0.83	0.48	0.44	0.36	4.87	0.31	0.32	0.22	0.02	0.00	0.15	2.00	2.18	0.43	0.24	0.66	0.34	0.20
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 206k	26	Rapid	1433.00	110.46	38.21	82.84	0.84	5.11	0.82	9.45	4.80	1.19	0.36	0.55	0.50	0.15	0.30	0.11	2.56	0.17	0.14	0.05	0.01	0.00	0.06	1.48	1.02	0.27	0.04	0.21	0.17	0.09
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe207k	26	Rapid	1338.18	93.04	41.64	60.68	3.59	5.83	0.90	9.55	7.85	1.58	0.30	0.62	0.40	0.11	0.27	0.24	3.21	0.16	0.11	0.09	0.02	0.00	0.16	1.91	0.90	0.23	0.07	0.24	0.17	0.08
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe176	30	Rapid	1588.00	140.34	23.09	96.27	0.96	3.78	1.01	8.08	2.58	0.92	0.40	0.65	0.33	0.51	0.19	0.47	2.83	0.12	0.07	0.02	0.01	0.00	0.12	0.91	0.27	0.39	0.58	0.22	0.25	0.13
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe177	30	Rapid	1011.21	114.84	26.63	79.06	1.21	5.77	1.19	11.18	7.10	2.04	0.34	0.96	0.38	0.38	0.19	0.46	4.00	0.16	0.18	0.20	0.00	0.00	0.13	1.46	2.93	0.54	0.11	0.53	0.45	0.27
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 178	30	Rapid	1311.42	121.00	47.93	84.88	1.30	4.51	0.54	8.33	3.59	1.65	0.26	0.64	0.31	0.26	0.14	0.31	2.91	0.16	0.09	0.07	0.01	0.00	0.12	1.34	0.53	0.36	0.16	0.24	0.23	0.13
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 179	30	Rapid	1961.79	88.70	53.86	39.52	9.02	10.91	0.27	7.23	7.99	2.05	0.43	1.07	1.65	0.32	0.11	0.43	10.79	0.16	0.08	0.04	0.03	0.00	0.09	1.52	1.09	0.24	0.24	0.23	0.16	0.07
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 208k	30	Rapid	1320.46	112.55	43.86	68.06	2.93	7.41	1.12	9.56	6.43	0.78	0.45	0.69	0.33	0.50	0.20	0.64	5.29	0.21	0.13	0.08	0.02	0.00	0.19	1.68	1.11	0.33	0.22	0.25	0.29	0.13
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 209k	30	Rapid	2188.75	160.69	68.56	115.72	1.97	10.49	2.27	20.47	16.85	2.15	0.42	1.73	0.73	0.78	0.56	0.43	5.81	0.31	0.23	0.07	0.02	0.00	0.13	1.93	1.69	0.66	0.13	0.58	0.73	0.37
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 211k	30	Rapid	1058.70	81.08	35.34	56.38	2.09	5.08	0.89	7.39	4.32	0.67	0.26	0.43	0.27	0.23	0.12	0.16	1.66	0.12	0.10	0.05	0.02	0.00	0.06	0.86	1.72	0.27	0.08	0.17	0.23	0.09
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 184	34	Rapid	1556.32	119.29	56.44	68.48	1.19	6.54	0.84	7.40	4.47	0.64	0.28	0.70	0.43	0.36	0.17	0.97	4.80	0.22	0.13	0.06	0.01	0.00	0.18	0.96	0.41	0.45	0.07	0.29	0.35	0.18
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 186	34	Rapid	1050.29	96.24	45.41	66.15	0.62	6.10	1.39	7.44	5.50	0.91	0.26	0.82	0.28	0.36	0.18	1.42	5.47	0.48	0.26	0.08	0.01	0.01	0.15	1.33	0.59	0.14	0.14	0.66	0.74	0.41
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 187	34	Rapid	1936.00	121.17	70.89	78.51	4.08	7.12	0.60	12.81	5.18	1.73	0.36	0.74	0.60	0.32	0.20	0.48	6.02	0.27	0.09	0.07	0.02	0.00	0.09	1.61	2.11	0.36	0.13	0.27	0.28	0.12
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe214	34	Rapid	1524.17	100.34	57.93	68.12	1.11	4.72	1.00	8.98	8.27	0.81	0.31	0.85	0.25	0.33	0.15	1.02	2.89	0.34	0.22	0.05	0.01	0.00	0.11	1.11	1.02	0.48	0.08	0.50	0.54	0.27
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 215	34	Rapid	924.43	77.87	48.29	58.19	1.17	6.54	1.36	3.91	8.91	1.18	0.15	0.76	0.36	0.41	0.21	0.70	4.80	0.30	0.19	0.10	0.01	0.00	0.14	0.88	0.77	0.41	0.12	0.37	0.52	0.26
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 216k	34	Rapid	1457.58	104.72	29.34	72.40	4.74	7.86	1.60	3.11	7.86	1.33	0.57	0.95	0.49	0.45	0.27	4.86	10.20	0.71	0.35	0.09	0.04	0.06	0.12	0.98	0.67	0.37	0.09	0.63	0.82	0.41
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 217k	34	Rapid	1459.87	79.88	57.29	47.94	3.80	6.45	1.43	5.12	8.49	1.04	0.35	1.55	0.61	0.26	0.34	5.48	8.19	0.40	0.21	0.11	0.02	0.04	0.11	1.65	1.21	0.34	0.29	0.58	0.62	0.34
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 188	36	Rapid	1024.38	122.90	34.90	80.02	0.62	5.59	1.06	1.54	4.47	1.14	0.18	0.94	0.53	0.06	0.21	3.40	6.40	0.40	0.24	0.10	0.01	0.08	0.11	1.44	0.46	0.58	0.14	0.58	0.54	0.33
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 189	36	Rapid	982.29	102.19	47.64	75.11	1.31	7.56	1.36	11.00	13.26	0.84	0.31	1.07	0.53	0.31	0.36	0.62	5.23	0.24	0.18	0.16	0.01	0.00	0.19	1.07	1.02	0.56	0.17	0.47	0.45	0.23
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 190	36	Rapid	212.95	12.97	9.73	9.58	0.13	0.99	0.25	0.40	1.00	0.20	0.06	0.22	0.05	0.06	0.04	0.85	1.24	0.07	0.06	0.03	0.00	0.02	0.02	0.27	0.09	0.06	0.04	0.10	0.11	0.07
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 191	36	Rapid	841.84	76.29	39.96	43.27	1.36	5.78	1.56	2.76	8.02	0.96	0.17	1.21	0.56	0.28	0.17	3.23	7.89	0.50	0.29	0.12	0.01	0.02	0.10	0.72	0.42	0.38	0.11	0.83	0.88	0.50
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 218k	36	Rapid	1412.36	122.02	53.48	73.08	3.12	13.78	2.99	10.26	13.66	2.29	1.17	1.61	0.77	0.82	0.36	1.44	11.01	0.72	0.40	0.08	0.03	0.00	0.20	1.80	0.97	0.53	0.17	0.89	1.06	0.47
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 220k	36	Rapid	1695.69	88.40	29.12	67.17	4.16	8.20	5.51	7.32	21.57	1.10	2.00	2.45	0.67	1.22	0.43	2.95	13.35	1.30	1.34	0.47	0.04	0.00	0.07	1.54	0.57	0.22	0.12	2.95	3.33	2.00
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 221k	36	Rapid	876.76	60.30	37.81	43.29	0.60	6.64	1.55	1.71	7.92	1.13	0.24	1.29	0.55	0.37	0.26	2.57	7.14	0.65	0.50	0.17	0.01	0.03	0.09	1.05	0.33	0.15	0.05	1.25	1.09	0.60
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 192	37	Rapid	335.07	18.41	10.57	15.54	0.18	1.10	0.27	0.93	1.04	0.17	0.07	0.20	0.07	0.10	0.05	1.21	2.06	0.15	0.06	0.02	0.01	0.01	0.03	0.26	0.21	0.08	0.04	0.11	0.15	0.07
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 194	37	Rapid	1854.86	126.68	63.90	71.45	0.97	6.86	1.02	2.28	5.22	0.57	0.40	1.03	0.71	0.14	0.17	5.68	15.07	0.48	0.24	0.06	0.02	0.04	0.14	0.82	1.20	0.38	0.10	0.57	0.71	0.40
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 195	37	Rapid	323.54	23.22	8.13	14.55	1.83	3.30	0.28	1.54	2.18	1.02	0.05	0.34	0.28	0.41	0.05	1.07	5.23	0.09	0.04	0.01	0.01	0.00	0.02	0.24	0.39	0.15	0.17	0.12	0.14	0.07
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 225	37	Rapid	980.14	69.76	25.87	50.42	5.75	10.26	2.43	7.53	18.80	3.10	0.32	1.71	0.68	0.83	0.17	0.87	7.54	0.33	0.19	0.12	0.02	0.00	0.17	0.87	1.76	0.44	0.17	0.63	0.86	0.42
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 227	37	Rapid	1122.71	70.90	36.95	50.22	4.51	8.56	1.37	6.38	9.77	1.60	0.14	0.78	0.43	0.54	0.12	1.33	6.22	0.24	0.17	0.08	0.03	0.00	0.15	0.65	1.11	0.46	0.10	0.45	0.53	0.30
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 193	37	Rapid	1047.46	97.26	47.12	65.77	0.72	5.17	1.46	10.18	9.83	2.52	0.28	1.05	0.46	0.12	0.09	0.46	3.23	0.28	0.15	0.09	0.01	0.00	0.11	1.03	1.28	0.44	0.12	0.39	0.38	0.21
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 223k	37	Rapid	996.50	57.37	32.71	44.14	2.36	6.82	1.39	1.43	10.16	1.33	0.22	1.70	0.80	0.34	0.26	2.59	10.37	0.47	0.28	0.11	0.02	0.02	0.11	0.58	1.06	0.32	0.19	0.66	0.87	0.43
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 1	22	Gradual	1475.30	78.45	69.37	47.87	0.53	5.15	1.11	12.04	3.29	1.85	0.25	1.08	0.36	0.31	0.14	0.05	1.84	0.16	0.12	0.07	0.02	0.00	0.05	1.36	0.51	0.35	0.04	0.31	0.21	0.14
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 2	22	Gradual	1224.45	80.10	49.54	51.06	0.72	3.91	0.47	9.31	2.04	1.16	0.28	1.23	0.26	0.10	0.19	0.03	1.05	0.21	0.11	0.05	0.02	0.00	0.04	1.31	0.40	0.21	0.05	0.18	0.12	0.07
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 3	22	Gradual	185.30	12.40	10.83	7.46	0.12	0.60	0.12	1.99	0.27	0.33	0.06	0.12	0.03	0.07	0.04	0.02	0.15	0.02	0.02	0.02	0.00	0.00	0.00	0.33	0.18	0.04	0.07	0.03	0.04	0.02
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 4	22	Gradual	1838.35	79.38	55.00	49.98	0.97	4.86	0.90	12.81	2.51	0.88	0.94	0.60	0.26	0.24	0.07	0.05	1.91	0.13	0.09	0.04	0.01	0.00	0.05	1.16	0.40	0.27	0.06	0.20	0.15	0.08
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe250	22	Gradual	2305.37	156.92	57.71	119.27	1.26	7.94	0.83	17.56	4.43	0.74	0.35	0.95	0.46	0.32	0.22	0.09	3.31	0.13	0.05	0.12	0.02	0.00	0.11	1.56	1.60	0.50	0.16	0.21	0.19	0.07
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe251	22	Gradual	999.60	83.56	35.69	58.22	0.93	4.12	0.42	6.51	1.86	0.49	0.17	0.55	0.28	0.22	0.11	0.01	1.65	0.17	0.07	0.05	0.01	0.00	0.01	0.51	0.92	0.20	0.11	0.14	0.17	0.09
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe268k	22	Gradual	1736.27	82.68	54.15	54.78	2.89	6.62	1.09	15.33	2.61	1.72	0.46	0.85	0.41	0.59	0.14	0.23	3.28	0.16	0.11	0.07	0.02	0.00	0.11	1.88	1.10	0.30	0.13	0.16	0.13	0.06
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe269k	22	Gradual	1632.91	82.78	53.56	60.68	0.54	4.40	0.59	13.34	2.54	1.61	0.24	0.96	0.30	0.26	0.23	0.07	1.50	0.18	0.16	0.06	0.01	0.00	0.06	1.64	0.71	0.38	0.05	0.29	0.21	0.12
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe270k	22	Gradual	1050.17	86.12	41.81	64.38	0.95	3.96	0.59	11.54	2.14	0.85	0.23	0.71	0.23	0.15	0.13	0.06	1.10	0.10	0.07	0.07	0.01	0.00	0.03	1.28	0.67	0.23	0.11	0.16	0.12	0.06
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe271k	22	Gradual	1146.29	85.97	41.43	59.61	1.41	5.75	0.45	8.32	1.82	0.66	0.43	0.49	0.39	0.12	0.11	0.08	1.50	0.11	0.07	0.09	0.01	0.00	0.04	0.90	0.87	0.25	0.06	0.18	0.17	0.11
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe290k	22	Gradual	1715.89	118.26	48.86	88.46	0.65	7.74	0.86	12.00	3.50	1.94	0.30	0.58	0.38	0.24	0.19	0.12	1.89	0.32	0.16	0.05	0.01	0.00	0.13	1.41	1.24	0.39	0.11	0.34	0.36	0.17
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe291k	22	Gradual	345.98	25.41	6.45	20.26	0.94	1.57	0.28	3.58	1.00	1.00	0.24	0.31	0.09	0.15	0.14	0.02	0.69	0.03	0.07	0.04	0.01	0.00	0.00	0.70	0.42	0.09	0.05	0.10	0.06	0.04
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe6	26	Gradual	345.69	12.75	11.34	14.17	0.72	1.90	0.64	4.31	1.27	0.22	0.11	0.36	0.13	1.03	0.07	0.09	0.60	0.05	0.03	0.01	0.01	0.00	0.00	0.34	0.44	0.03	0.15	0.11	0.13	0.10
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe He7	26	Gradual	803.61	56.03	30.37	37.74	0.56	3.89	0.94	5.80	3.43	1.10	0.28	0.63	0.40	0.18	0.25	0.19	1.77	0.19	0.16	0.08	0.01	0.00	0.07	0.79	0.93	0.28	0.06	0.33	0.28	0.16
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 8	26	Gradual	1013.14	68.36	45.78	43.69	0.74	2.52	0.27	6.27	2.15	0.82	0.14	0.46	0.30	0.00	0.13	0.05	0.82	0.11	0.08	0.04	0.00	0.00	0.04	0.98	1.01	0.30	0.04	0.15	0.12	0.09
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe254	26	Gradual	183.98	18.39	6.33	15.53	0.24	1.08	0.21	2.33	0.60	0.24	0.09	0.13	0.05	0.10	0.08	0.04	0.66	0.04	0.03	0.00	0.00	0.00	0.02	0.35	0.20	0.04	0.04	0.03	0.03	0.02
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe255	26	Gradual	2511.05	109.45	93.59	86.66	1.40	7.24	0.57	19.68	4.91	0.81	0.27	0.89	0.52	0.40	0.19	0.06	1.89	0.31	0.10	0.07	0.01	0.00	0.08	1.66	1.07	0.41	0.06	0.29	0.30	0.14
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe272k	26	Gradual	1042.35	78.82	49.11	57.75	0.88	4.63	1.00	9.93	1.80	1.54	0.40	0.48	0.34	0.40	0.13	0.06	1.36	0.10	0.09	0.07	0.02	0.00	0.04	1.20	1.94	0.18	0.30	0.14	0.12	0.07
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe273k	26	Gradual	262.91	24.01	5.99	17.86	0.28	1.03	0.17	3.21	0.61	0.33	0.10	0.19	0.06	0.04	0.08	0.02	0.33	0.05	0.04	0.03	0.00	0.00	0.01	0.39	0.36	0.05	0.05	0.05	0.04	0.03
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe274k	26	Gradual	869.35	64.58	40.55	56.30	0.58	2.74	0.71	8.14	2.00	0.38	0.13	0.36	0.30	0.28	0.21	0.08	0.58	0.16	0.12	0.10	0.00	0.00	0.04	1.02	0.48	0.23	0.07	0.16	0.17	0.09
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe275k	26	Gradual	1565.07	76.91	69.29	61.10	2.00	6.09	1.20	12.76	5.71	1.09	0.62	0.70	0.30	0.49	0.22	0.46	4.86	0.36	0.43	0.11	0.04	0.00	0.14	1.75	0.20	0.40	0.10	0.53	0.67	0.29
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe292k	26	Gradual	1622.21	94.10	48.61	67.84	0.88	7.61	1.25	15.72	7.63	0.78	0.40	0.78	0.32	0.34	0.26	0.24	3.57	0.12	0.17	0.07	0.01	0.00	0.10	1.53	0.63	0.37	0.16	0.29	0.26	0.12
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe293k	26	Gradual	1210.84	93.73	62.96	50.62	1.04	5.27	0.89	12.95	3.59	1.09	0.39	0.83	0.39	0.19	0.17	0.10	2.14	0.17	0.14	0.06	0.01	0.00	0.06	1.71	1.00	0.33	0.07	0.24	0.19	0.11
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 12	30	Gradual	877.89	86.16	53.20	56.69	1.67	7.29	1.46	11.73	13.57	1.60	0.62	1.61	0.58	0.44	0.36	0.33	4.26	0.20	0.20	0.17	0.02	0.00	0.15	1.45	2.31	0.56	0.09	0.41	0.42	0.26
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 9	30	Gradual	912.37	91.62	50.09	86.11	0.87	8.05	1.92	12.37	11.92	4.81	0.36	2.09	0.69	0.64	0.60	0.15	3.84	0.22	0.27	0.34	0.01	0.00	0.13	2.13	2.07	0.54	0.52	0.61	0.51	0.39
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 258	30	Gradual	1245.98	128.19	57.71	89.85	1.35	4.47	1.32	10.58	6.11	0.46	0.71	0.69	0.40	0.24	0.28	0.44	2.10	0.15	0.27	0.10	0.01	0.00	0.09	1.29	2.48	0.63	0.35	0.35	0.33	0.18
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 10	30	Gradual	1301.39	78.14	63.17	46.14	1.17	7.12	2.21	14.54	18.91	2.59	0.59	1.52	0.66	0.51	0.28	0.19	4.20	0.31	0.27	0.19	0.01	0.00	0.14	1.29	2.23	0.68	0.43	0.85	0.72	0.42
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 11	30	Gradual	363.96	48.41	41.51	39.52	0.89	5.27	1.43	10.53	6.99	1.80	0.47	1.38	0.30	0.60	0.29	0.13	2.68	0.33	0.32	0.31	0.01	0.00	0.00	1.47	0.53	0.39	0.21	0.46	0.40	0.38
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe259k	30	Gradual	981.16	73.91	43.76	63.88	0.58	4.74	0.64	11.04	6.59	1.77	0.14	0.82	0.38	0.23	0.12	0.07	2.09	0.19	0.09	0.08	0.01	0.00	0.06	1.30	0.84	0.30	0.06	0.29	0.30	0.15
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 294k	30	Gradual	843.89	63.77	48.92	66.05	1.21	5.30	0.94	9.03	13.11	1.61	0.23	1.38	0.69	0.82	0.42	0.22	2.90	0.32	0.15	0.16	0.02	0.00	0.11	1.25	3.02	0.50	0.52	0.42	0.40	0.25
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe276k	30	Gradual	1040.09	88.27	38.45	69.36	0.68	4.21	0.66	11.22	5.50	3.03	0.29	0.61	0.33	0.30	0.12	0.05	1.47	0.13	0.10	0.04	0.01	0.00	0.05	1.17	1.22	0.31	0.09	0.20	0.23	0.11
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe277k	30	Gradual	1280.31	90.02	84.87	63.01	1.30	6.29	0.97	16.16	5.29	1.58	0.58	1.14	0.66	0.29	0.31	0.13	3.19	0.29	0.20	0.08	0.02	0.00	0.15	1.83	0.98	0.35	0.10	0.31	0.31	0.18
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe278k	30	Gradual	603.76	38.40	29.51	29.14	0.36	2.75	0.81	5.40	3.80	2.24	0.30	0.93	0.30	0.25	0.17	0.12	1.83	0.19	0.14	0.07	0.01	0.00	0.14	0.86	0.37	0.28	0.09	0.22	0.20	0.11
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe279k	30	Gradual	1039.92	43.52	41.28	44.23	5.07	5.47	2.29	12.34	19.64	2.27	0.45	0.86	0.32	0.77	0.32	0.47	5.01	0.29	0.30	0.11	0.04	0.00	0.09	1.42	1.59	0.51	0.34	0.63	0.91	0.43
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe295k	30	Gradual	1100.42	141.56	61.39	90.90	1.22	10.03	3.63	16.57	21.02	3.37	0.81	2.83	0.96	1.21	0.61	0.96	7.54	0.47	0.62	0.40	0.01	0.00	0.23	2.39	1.26	0.62	0.05	1.28	0.96	0.55
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 18	34	Gradual	1126.03	54.71	49.76	47.01	4.43	9.62	1.51	12.83	19.61	1.19	0.15	1.10	0.67	0.56	0.14	0.75	7.36	0.29	0.19	0.03	0.04	0.00	0.26	0.83	0.91	0.34	0.20	0.39	0.47	0.23
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 19	34	Gradual	693.35	53.96	50.81	41.36	1.01	7.75	1.86	12.93	21.29	0.89	0.19	1.29	0.59	0.20	0.38	0.18	4.82	0.21	0.19	0.11	0.01	0.00	0.10	1.10	1.16	0.46	0.07	0.53	0.54	0.32
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 20	34	Gradual	755.13	87.82	32.49	78.33	12.38	11.14	3.30	17.00	24.68	1.12	0.42	1.08	0.54	1.39	0.18	0.42	7.03	0.27	0.22	0.08	0.03	0.00	0.20	0.88	2.10	0.43	0.07	1.28	1.30	0.71
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe262	34	Gradual	1032.34	79.05	36.85	71.08	5.80	9.03	1.51	17.55	39.05	1.01	0.35	2.14	0.39	0.78	0.17	0.57	6.61	0.19	0.16	0.08	0.04	0.00	0.22	1.22	1.89	0.46	0.13	0.48	0.51	0.30
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe263	34	Gradual	308.67	18.14	19.48	18.76	0.77	2.22	0.52	5.02	6.99	0.15	0.10	0.30	0.20	0.20	0.07	0.18	1.52	0.10	0.07	0.01	0.01	0.00	0.07	0.33	0.47	0.18	0.04	0.14	0.14	0.07
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	OeHe 17	34	Gradual	974.45	94.31	41.64	90.60	2.74	7.88	1.91	12.41	29.25	2.37	0.25	1.31	0.76	0.73	0.15	0.11	5.21	0.20	0.13	0.12	0.04	0.00	0.13	0.22	1.73	0.49	0.24	0.42	0.65	0.38
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 289k	34	Gradual	696.91	75.12	26.40	72.01	9.98	8.62	1.69	16.10	24.03	0.59	0.19	0.98	0.37	1.13	0.13	0.30	5.92	0.21	0.13	0.06	0.03	0.00	0.18	0.96	0.92	0.31	0.15	0.62	0.55	0.33
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Ow285k	34	Gradual	235.14	14.39	16.11	12.61	0.73	2.26	0.46	3.68	5.14	0.44	0.11	0.38	0.07	0.13	0.09	0.08	1.34	0.06	0.06	0.05	0.01	0.00	0.05	0.39	0.69	0.08	0.09	0.08	0.10	0.06
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Ow286k	34	Gradual	1364.27	72.25	72.70	74.92	21.75	15.06	3.21	23.09	45.23	9.15	0.61	3.18	0.79	2.83	0.43	1.02	14.86	1.36	0.63	0.18	0.08	0.00	0.40	2.37	1.89	1.09	0.21	2.16	3.36	1.42
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe287k	34	Gradual	192.68	18.14	8.17	16.06	1.49	2.32	0.47	3.85	3.49	0.56	0.11	0.29	0.12	0.19	0.10	0.07	1.17	0.15	0.07	0.06	0.01	0.00	0.04	0.43	0.41	0.13	0.11	0.10	0.12	0.08
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe288k	34	Gradual	796.99	82.07	53.93	64.37	3.52	8.00	1.06	15.09	18.01	1.74	0.37	0.71	0.56	0.52	0.14	0.27	4.48	0.19	0.14	0.05	0.03	0.00	0.14	0.66	1.12	0.30	0.27	0.29	0.28	0.16
Laboratory experiment	Ostrea edulis	urn:lsid:marinespecies.org:taxname:140658	marinespecies.org	Oe 284k	34	Gradual	1078.34	65.94	65.00	60.94	0.92	5.12	0.59	10.50	11.04	0.69	0.13	0.51	0.49	0.14	0.12	0.09	3.17	0.16	0.14	0.05	0.02	0.00	0.12	0.44	1.15	0.38	0.20	0.17	0.19	0.13