Stief Peter 0000-0002-6355-150X Danish Center for Hadal Research HADAL 2025 Degradation of diatom-derived dissolved organic matter (DOM) in surface seawater dataset https://doi.org/10.1594/PANGAEA.974879 2025-08-03T00:01:13 Stored surface seawater originally collected in the North Sea was amended with dissolved organic matter (DOM) obtained from the diatom Skeletonema marinoi by exposing the diatom culture to a hydrostatic pressure level of 40 MPa for 24 h. To this end, S. marinoi had been grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. Diatom-cell-free DOM was added to aliquoted seawater samples at initial concentrations of 250 µmol C/L. The microbial degradation of the added DOM and the microbial response to DOM-amendment at 15°C and in darkness was followed for 2 weeks. The seawater was subsampled at defined time intervals to analyze a number of variables. Stief Peter 0000-0002-6355-150X Niggemann Jutta jutta.niggemann@uni-oldenburg.de 0000-0003-1237-9233 Bligh Margot mbligh@mpi-bremen.de 0000-0002-7945-7956 Buck-Wiese Hagen buckwies@usc.edu 0000-0002-4807-5795 Schauberger Clemens schauberger@biology.sdu.dk 0000-0002-2097-4959 Wünsch Urban 0000-0001-6972-6932 Steinke Michael msteinke@essex.ac.uk 0000-0001-6820-0154 Hehemann Jan-Hendrik jhheheman@marum.de 0000-0002-8700-2564 Glud Ronnie N rnglud@biology.sdu.dk 0000-0002-7069-893X in prep. Pressure-induced leakage of dissolved organic matter from sinking diatom aggregates Stief Peter 0000-0002-6355-150X Schauberger Clemens schauberger@biology.sdu.dk 0000-0002-2097-4959 Becker Kevin W kbecker@geomar.de 0000-0001-6317-1884 Elvert Marcus melvert@marum.de 0000-0003-3863-0975 Balmonte John Paul jpb422@lehigh.edu 0000-0001-5571-4893 Franco-Cisterna Belén belen@biology.sdu.dk 0000-0002-9114-2818 Middelboe Mathias 0000-0002-9587-9171 Glud Ronnie N rnglud@biology.sdu.dk 0000-0002-7069-893X 2023 Hydrostatic pressure induces transformations in the organic matter and microbial community composition of marine snow particles journal article Communications Earth & Environment 4(1) https://doi.org/10.1038/s43247-023-01045-4 377 7.841 7.841 54.155 54.155 7.841 54.155 2019-04-07T12:00:00 2019-04-07T13:00:00 uncoded table Danish National Research Foundation DG http://dg.dk/en/ https://doi.org/10.13039/501100001732 DNRF145 Danish Center for Hadal Research, HADAL SW_042019 54.155 7.841 2019-04-07T12:00:00 2019-04-07T13:00:00 CTD/Rosette CTD-RO CTD http://vocab.nerc.ac.uk/collection/L05/current/130/ CTD & XBT Water samplers 1424 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Information about the used cultured organisms: Skeletonema marinoi, Bigelow National Center for Marine Algae and Microbiota, Strain No. CCMP1332, isolated from Long Island Sound (41.2264 N, -73.0639 W) (isolation date is unknown); https://ncma.bigelow.org/CCMP1332 Type of study Study type type Stief Peter 0000-0002-6355-150X Study type Date/time start, experiment Date/time start exp Stief Peter 0000-0002-6355-150X Date/time start exp Date/time end, experiment Date/time end exp Stief Peter 0000-0002-6355-150X Date/time end exp Species, unique identification Species UID species Stief Peter 0000-0002-6355-150X Species UID Treatment: carbon T:C µmol/l treatment carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 Molar Concentration Stief Peter 0000-0002-6355-150X Diatom-derived dissolved organic matter (DOM) was added at the start of the incubation T:C [µmol/l] Treatment: duration T:duration days treatment duration http://purl.obolibrary.org/obo/PATO_0001309 Time T http://dbpedia.org/resource/Time Stief Peter 0000-0002-6355-150X Incubation time T:duration [days] Carbon, organic, dissolved DOC µmol/l carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 organic dissolved http://purl.obolibrary.org/obo/PATO_0001986 Molar Concentration Total organic carbon analyzer, Shimadzu, TOC-L Shimadzu TOC-L Total Organic Carbon Analyser series http://vocab.nerc.ac.uk/collection/L22/current/TOOL1760/ Stief Peter 0000-0002-6355-150X Concentration of dissolved organic carbon (DOC) in seawater DOC [µmol/l] Nitrogen, total dissolved TDN µmol/l nitrogen N https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25555 Molar Concentration Total organic carbon analyzer, Shimadzu, TOC-L Shimadzu TOC-L Total Organic Carbon Analyser series http://vocab.nerc.ac.uk/collection/L22/current/TOOL1760/ Stief Peter 0000-0002-6355-150X Concentration of total dissolved nitrogen (TDN) in seawater TDN [µmol/l] Proteins, total TPRT mg/l protein http://purl.obolibrary.org/obo/CHEBI_36080 total https://www.oxforddictionaries.com/definition/american_english/total Density http://dbpedia.org/resource/Density Lowry assay after Lowry et al. 1951 Stief Peter 0000-0002-6355-150X Concentration of total dissolved proteins in seawater TPRT [mg/l] Polysaccharides Polysacch µg/ml Density http://dbpedia.org/resource/Density PAHBAH reducing sugar assay (Lever 1972) Stief Peter 0000-0002-6355-150X Concentration of total dissolved polysaccharides in seawater Polysacch [µg/ml] Bacteria Bact #/ml Bacteria https://www.marinespecies.org/aphia.php?p=taxdetails&id=6 Fluorescence determination of DAPI stain Stief Peter 0000-0002-6355-150X Automated microscopic cell counts and image analysis using ACMEtool3 (Bennke et al. 2016) Bact [#/ml] Respiration rate, oxygen Resp O2 µmol/l/day respiration rate http://www.oxfordreference.com/view/10.1093/oi/authority.20110803100415939?rskey=tF1NrR&result=2 oxygen O https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25805 Reaction Rate Oxygen logger, FireSting, Pyroscience Stief Peter 0000-0002-6355-150X Optode patches on the inner wall of the glass vials were interrogated from outside using a FireSting Oxygen logger (Pyroscience, Aachen, Germany) Resp O2 [µmol/l/day] CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable culture experiment diatoms DOM DOM degradation hydrostatic pressure Laboratory experiment leakage microbial community seawater author100463 author100464 author10758 author27446 author28319 author36756 author41880 author47574 author55884 author72212 author74060 author74284 author75907 author94312 award1575 curlevel30 event3219751 funder21052 geocode1599 geocode1600 geocode1601 inst21194 journal17037 license21 method10616 method10923 method17565 method17566 method17569 method4969 param105161 param185520 param27325 param2955 param510679 param516119 param516120 param520660 param536897 param536904 param6205 param766 pi36756 ref119164 ref131791 term1061861 term1073493 term1073807 term1080072 term2299371 term2735579 term38253 term38477 term38494 term38497 term38548 term38841 term41013 term43792 term43975 term44030 term44031 term44419 term47460 term47583 term47971 term49384 term49506 term54685 term60989 term67626 term68026 term72626 term738 term75703 topotype11