de.pangaea.dataset974879 und dataset PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact 2026-04-30T21:03:03Z https://doi.pangaea.de/10.1594/PANGAEA.974879 <gco:CharacterString>Degradation of diatom-derived dissolved organic matter (DOM) in surface seawater</gco:CharacterString> 2025-08-03T00:01:13 publication https://doi.org/10.1594/PANGAEA.974879 Stief, Peter https://orcid.org/0000-0002-6355-150X information author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital Stored surface seawater originally collected in the North Sea was amended with dissolved organic matter (DOM) obtained from the diatom Skeletonema marinoi by exposing the diatom culture to a hydrostatic pressure level of 40 MPa for 24 h. To this end, S. marinoi had been grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. Diatom-cell-free DOM was added to aliquoted seawater samples at initial concentrations of 250 µmol C/L. The microbial degradation of the added DOM and the microbial response to DOM-amendment at 15°C and in darkness was followed for 2 weeks. The seawater was subsampled at defined time intervals to analyze a number of variables. completed Stief, Peter https://orcid.org/0000-0002-6355-150X information principalInvestigator culture experiment diatoms DOM DOM degradation hydrostatic pressure Laboratory experiment leakage microbial community seawater theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation 7.841 7.841 54.155 54.155 2019-04-07T12:00:00 2019-04-07T13:00:00 Information about the used cultured organisms: Skeletonema marinoi, Bigelow National Center for Marine Algae and Microbiota, Strain No. CCMP1332, isolated from Long Island Sound (41.2264 N, -73.0639 W) (isolation date is unknown); https://ncma.bigelow.org/CCMP1332 Type of study thematicClassification Date/time start, experiment thematicClassification Date/time end, experiment thematicClassification Species, unique identification thematicClassification Treatment: carbon physicalMeasurement Diatom-derived dissolved organic matter (DOM) was added at the start of the incubation µmol/l µmol/l Treatment: duration physicalMeasurement Incubation time days days Carbon, organic, dissolved physicalMeasurement Total organic carbon analyzer, Shimadzu, TOC-L; Concentration of dissolved organic carbon (DOC) in seawater µmol/l µmol/l Nitrogen, total dissolved physicalMeasurement Total organic carbon analyzer, Shimadzu, TOC-L; Concentration of total dissolved nitrogen (TDN) in seawater µmol/l µmol/l Proteins, total physicalMeasurement Lowry assay after Lowry et al. 1951; Concentration of total dissolved proteins in seawater mg/l mg/l Polysaccharides physicalMeasurement PAHBAH reducing sugar assay (Lever 1972); Concentration of total dissolved polysaccharides in seawater µg/ml µg/ml Bacteria physicalMeasurement Fluorescence determination of DAPI stain; Automated microscopic cell counts and image analysis using ACMEtool3 (Bennke et al. 2016) #/ml #/ml Respiration rate, oxygen physicalMeasurement Oxygen logger, FireSting, Pyroscience; Optode patches on the inner wall of the glass vials were interrogated from outside using a FireSting Oxygen logger (Pyroscience, Aachen, Germany) µmol/l/day µmol/l/day text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.974879?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: SW_042019 * LATITUDE: 54.155000 * LONGITUDE: 7.841000 * DATE/TIME START: 2019-04-07T12:00:00 * DATE/TIME END: 2019-04-07T13:00:00 * METHOD/DEVICE: CTD/Rosette 2019-04-07T12:00:00 Supplement to <gco:CharacterString>Pressure-induced leakage of dissolved organic matter from sinking diatom aggregates</gco:CharacterString> Stief, Peter https://orcid.org/0000-0002-6355-150X information author Niggemann, Jutta
jutta.niggemann@uni-oldenburg.de
https://orcid.org/0000-0003-1237-9233 information author Bligh, Margot
mbligh@mpi-bremen.de
https://orcid.org/0000-0002-7945-7956 information author Buck-Wiese, Hagen
buckwies@usc.edu
https://orcid.org/0000-0002-4807-5795 information author Schauberger, Clemens
schauberger@biology.sdu.dk
https://orcid.org/0000-0002-2097-4959 information author Wünsch, Urban https://orcid.org/0000-0001-6972-6932 information author Steinke, Michael
msteinke@essex.ac.uk
https://orcid.org/0000-0001-6820-0154 information author Hehemann, Jan-Hendrik
jhheheman@marum.de
https://orcid.org/0000-0002-8700-2564 information author Glud, Ronnie N
rnglud@biology.sdu.dk
https://orcid.org/0000-0002-7069-893X information author documentHardcopy Related to <gco:CharacterString>Hydrostatic pressure induces transformations in the organic matter and microbial community composition of marine snow particles</gco:CharacterString> 2023-01-01 publication https://doi.org/10.1038/s43247-023-01045-4 Stief, Peter https://orcid.org/0000-0002-6355-150X information author Schauberger, Clemens
schauberger@biology.sdu.dk
https://orcid.org/0000-0002-2097-4959 information author Becker, Kevin W
kbecker@geomar.de
https://orcid.org/0000-0001-6317-1884 information author Elvert, Marcus
melvert@marum.de
https://orcid.org/0000-0003-3863-0975 information author Balmonte, John Paul
jpb422@lehigh.edu
https://orcid.org/0000-0001-5571-4893 information author Franco-Cisterna, Belén
belen@biology.sdu.dk
https://orcid.org/0000-0002-9114-2818 information author Middelboe, Mathias https://orcid.org/0000-0002-9587-9171 information author Glud, Ronnie N
rnglud@biology.sdu.dk
https://orcid.org/0000-0002-7069-893X information author documentDigital Communications Earth & Environment 4(1) 377