PANGAEA_974879
Degradation of diatom-derived dissolved organic matter (DOM) in surface seawater
Stief, Peter
Degradation of diatom-derived dissolved organic matter (DOM) in surface seawater
2025-08-03
PANGAEA
Dataset
https://doi.pangaea.de/10.1594/PANGAEA.974879
Investigator
Peter
Stief
Earth Science
Type of study
Date/time start, experiment
Date/time end, experiment
Species, unique identification
Treatment: carbon
Treatment: duration
Carbon, organic, dissolved
Nitrogen, total dissolved
Proteins, total
Polysaccharides
Bacteria
Respiration rate, oxygen
geoscientificInformation
CTD/Rosette
culture experiment
diatoms
DOM
DOM degradation
hydrostatic pressure
Laboratory experiment
leakage
microbial community
seawater
SW_042019
Total organic carbon analyzer, Shimadzu, TOC-L
Lowry assay after Lowry et al. 1951
PAHBAH reducing sugar assay (Lever 1972)
Fluorescence determination of DAPI stain
Oxygen logger, FireSting, Pyroscience
2019-04-07
2019-04-07
Complete
54.155
54.155
7.841
7.841
unrestricted
CC-BY-4.0: Creative Commons Attribution 4.0 International
English
PANGAEA
Data Publisher for Earth & Environmental Science
https://www.pangaea.de/
Data Center Contact
Michael
Diepenbroek
info@pangaea.de
Leobener Str.
Bremen
Bremen
28359
Germany
online
1424 data points
text/tab-separated-values
Stief, Peter; Niggemann, Jutta; Bligh, Margot; Buck-Wiese, Hagen; Schauberger, Clemens; Wünsch, Urban; Steinke, Michael; Hehemann, Jan-Hendrik; Glud, Ronnie N (in prep.): Pressure-induced leakage of dissolved organic matter from sinking diatom aggregates.
Stief, Peter; Schauberger, Clemens; Becker, Kevin W; Elvert, Marcus; Balmonte, John Paul; Franco-Cisterna, Belén; Middelboe, Mathias; Glud, Ronnie N (2023): Hydrostatic pressure induces transformations in the organic matter and microbial community composition of marine snow particles. Communications Earth & Environment, 4(1), 377, https://doi.org/10.1038/s43247-023-01045-4
Stored surface seawater originally collected in the North Sea was amended with dissolved organic matter (DOM) obtained from the diatom Skeletonema marinoi by exposing the diatom culture to a hydrostatic pressure level of 40 MPa for 24 h. To this end, S. marinoi had been grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. Diatom-cell-free DOM was added to aliquoted seawater samples at initial concentrations of 250 µmol C/L. The microbial degradation of the added DOM and the microbial response to DOM-amendment at 15°C and in darkness was followed for 2 weeks. The seawater was subsampled at defined time intervals to analyze a number of variables. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.974879"! ** Information about the used cultured organisms:
Skeletonema marinoi, Bigelow National Center for Marine Algae and Microbiota, Strain No. CCMP1332, isolated from Long Island Sound (41.2264 N, -73.0639 W) (isolation date is unknown); https://ncma.bigelow.org/CCMP1332
https://doi.org/10.1038/s43247-023-01045-4
Hydrostatic pressure induces transformations in the organic matter and microbial community composition of marine snow particles
DIF
9.4
2025-08-03
2026-04-30