/* DATA DESCRIPTION: Citation: Wyatt, Shea N; McNabb, Brandon J; Varela, Diana E (2025): Seawater carbonate chemistry and morphology and nutrient physiology of the cosmopolitan marine diatom Thalassiosira rotula [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.974439 Abstract: We investigated the effect of decreased pH on the morphology and nutrient physiology of the cosmopolitan marine diatom Thalassiosira rotula (CCMP3362) by acclimating unialgal cultures to two different CO2 gas concentrations under optimal light, temperature and nutrient conditions. This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were downloaded from UVic Research Data Collection (see Source) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2025-01-08. Keyword(s): Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Heterokontophyta; Laboratory experiment; Laboratory strains; Not applicable; Ocean acidification; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira rotula Related to: Wyatt, Shea N; McNabb, Brandon J; Varela, Diana E (2024): Morphological and physiological responses of the cosmopolitan marine diatom Thalassiosira rotula to acidification. Diatom Research, 39(2), 61-74, https://doi.org/10.1080/0269249X.2024.2369049 Source: Wyatt, Shea N; McNabb, Brandon J; Varela, Diana E (2024): Morphological and physiological responses of the cosmopolitan marine diatom Thalassiosira rotula to acidification [dataset]. Borealis, https://doi.org/10.5683/SP3/IUEQIN References: Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010 Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024 Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016 Related code / software: Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html Project(s): Ocean Acidification International Coordination Centre (OA-ICC) (URI: https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management) Parameter(s): Type of study (Study type) * PI: Varela, Diana E (dvarela@sfu.ca) Species, unique identification (Species UID) * PI: Varela, Diana E (dvarela@sfu.ca) Species, unique identification (URI) (Species UID (URI)) * PI: Varela, Diana E (dvarela@sfu.ca) Species, unique identification (Semantic URI) (Species UID (Semantic URI)) * PI: Varela, Diana E (dvarela@sfu.ca) Sample ID (Sample ID) * PI: Varela, Diana E (dvarela@sfu.ca) Treatment: partial pressure of carbon dioxide [µatm] (T:pCO2) * PI: Varela, Diana E (dvarela@sfu.ca) pH, total scale (pHT) * PI: Varela, Diana E (dvarela@sfu.ca) Growth rate [1/day] (µ) * PI: Varela, Diana E (dvarela@sfu.ca) Cell density [#/ml] (Cells) * PI: Varela, Diana E (dvarela@sfu.ca) Cell, length [µm] (Cell l) * PI: Varela, Diana E (dvarela@sfu.ca) Cell, diameter [µm] (Cell diam) * PI: Varela, Diana E (dvarela@sfu.ca) Cell surface area [µm**2] (Cell surface area) * PI: Varela, Diana E (dvarela@sfu.ca) Cell biovolume [µm**3] (Cell biovol) * PI: Varela, Diana E (dvarela@sfu.ca) Cell surface area/cell volume ratio [1/µm] (Cell SA/cell vol) * PI: Varela, Diana E (dvarela@sfu.ca) Chlorophyll a [µg/l] (Chl a) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon, organic, particulate [µmol/l] (POC) * PI: Varela, Diana E (dvarela@sfu.ca) Nitrogen, organic, particulate [µmol/l] (PON) * PI: Varela, Diana E (dvarela@sfu.ca) Biogenic silica [µmol/l] (bSiO2) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon/Nitrogen ratio [mol/mol] (C/N) * PI: Varela, Diana E (dvarela@sfu.ca) Silicon/Carbon, molar ratio (Si/C) * PI: Varela, Diana E (dvarela@sfu.ca) Silicon/Nitrogen, molar ratio (Si/N) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon, organic, particulate/Chlorophyll a ratio (POC/Chl a) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon utilization rate [µmol/l/day] (rhoC) * PI: Varela, Diana E (dvarela@sfu.ca) Nitrate utilization rate [µmol/l/day] (rhoNO3) * PI: Varela, Diana E (dvarela@sfu.ca) Silicon utilization rate [µmol/l/day] (rhoSi) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon utilization/nitrate utilization ratio (rhoC/rhoNO3) * PI: Varela, Diana E (dvarela@sfu.ca) Silicon utilization/carbon utilization ratio (rhoSi/rhoC) * PI: Varela, Diana E (dvarela@sfu.ca) Silicon utilization/nitrate utilization, ratio (rhoSi/rhoNO3) * PI: Varela, Diana E (dvarela@sfu.ca) Chlorophyll a [fg/µm**3] (Chl a) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon, organic, particulate [fg/µm**3] (POC) * PI: Varela, Diana E (dvarela@sfu.ca) Nitrogen, organic, particulate [fg/µm**3] (PON) * PI: Varela, Diana E (dvarela@sfu.ca) Biogenic silica quota, per volume [fmol/µm**3] (bSiO2/V) * PI: Varela, Diana E (dvarela@sfu.ca) Biogenic silica per surface area [fmol/µm*2] (bSiO2/SA) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon utilization rate, per volume [fmol/µm**3/day] (rhoC/V) * PI: Varela, Diana E (dvarela@sfu.ca) Nitrate utilization rate, per volume [fmol/µm**3/day] (rhoNO3/V) * PI: Varela, Diana E (dvarela@sfu.ca) Silicon utilization rate, per volume [fmol/µm**3/day] (rhoSi/V) * PI: Varela, Diana E (dvarela@sfu.ca) Chlorophyll a per cell [fmol/#] (Chl a/cell) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon, organic, particulate, per cell [ng/#] (POC/cell) * PI: Varela, Diana E (dvarela@sfu.ca) Nitrogen, organic, particulate, per cell [pg/#] (PON/cell) * PI: Varela, Diana E (dvarela@sfu.ca) Biogenic silica, per cell [fmol/#] (bSiO2/cell) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon utilization rate, per cell [pmol/#/day] (rhoC/cell) * PI: Varela, Diana E (dvarela@sfu.ca) Nitrate utilization rate, per cell [pmol/#/day] (rhoNO3/cell) * PI: Varela, Diana E (dvarela@sfu.ca) Silicon utilization rate, per cell [pmol/#/day] (rhoSi/cell) * PI: Varela, Diana E (dvarela@sfu.ca) Chlorophyll a, per mass carbon [µg/µmol] (Chl a/C) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon, organic, particulate, per mass carbon [µmol/µmol] (POC/C) * PI: Varela, Diana E (dvarela@sfu.ca) Nitrogen, organic, particulate, per mass carbon [µmol/µmol] (PON/C) * PI: Varela, Diana E (dvarela@sfu.ca) Biogenic silica quota, per mass carbon [µmol/µmol] (bSiO2/C) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon utilization rate, per mass carbon [µmol/µmol/day] (rhoC/C) * PI: Varela, Diana E (dvarela@sfu.ca) Nitrate utilization rate, per mass carbon [µmol/µmol/day] (rhoNO3/C) * PI: Varela, Diana E (dvarela@sfu.ca) Silicon utilization rate, per mass carbon [µmol/µmol/day] (rhoSi/C) * PI: Varela, Diana E (dvarela@sfu.ca) Temperature, water [°C] (Temp) * PI: Varela, Diana E (dvarela@sfu.ca) Salinity (Sal) * PI: Varela, Diana E (dvarela@sfu.ca) Phosphate [µmol/kg] ([PO4]3-) * PI: Varela, Diana E (dvarela@sfu.ca) Silicate [µmol/kg] (Si(OH)4) * PI: Varela, Diana E (dvarela@sfu.ca) pH, total scale (pHT) * PI: Varela, Diana E (dvarela@sfu.ca) * METHOD/DEVICE: Potentiometric pH, standard deviation [±] (pH std dev) * PI: Varela, Diana E (dvarela@sfu.ca) Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Varela, Diana E (dvarela@sfu.ca) Alkalinity, total [µmol/kg] (AT) * PI: Varela, Diana E (dvarela@sfu.ca) pH, total scale (pHT) * PI: Varela, Diana E (dvarela@sfu.ca) * METHOD/DEVICE: Calculated using CO2SYS Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Varela, Diana E (dvarela@sfu.ca) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: using CT and TA measurements Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Varela, Diana E (dvarela@sfu.ca) * METHOD/DEVICE: Calculated using CO2SYS * COMMENT: using only the TA and measured electrode pHT Carbonate system computation flag (CSC flag) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) pH, total scale (pHT) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) Carbon dioxide [µmol/kg] (CO2) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) Carbonate ion [µmol/kg] ([CO3]2-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) Aragonite saturation state (Omega Arg) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) Calcite saturation state (Omega Cal) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) License: Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 403 data points */ Study type Species UID Species UID (URI) Species UID (Semantic URI) Sample ID T:pCO2 [µatm] pHT µ [1/day] Cells [#/ml] Cell l [µm] Cell diam [µm] Cell surface area [µm**2] Cell biovol [µm**3] Cell SA/cell vol [1/µm] Chl a [µg/l] POC [µmol/l] PON [µmol/l] bSiO2 [µmol/l] C/N [mol/mol] Si/C Si/N POC/Chl a rhoC [µmol/l/day] rhoNO3 [µmol/l/day] rhoSi [µmol/l/day] rhoC/rhoNO3 rhoSi/rhoC rhoSi/rhoNO3 Chl a [fg/µm**3] POC [fg/µm**3] PON [fg/µm**3] bSiO2/V [fmol/µm**3] bSiO2/SA [fmol/µm*2] rhoC/V [fmol/µm**3/day] rhoNO3/V [fmol/µm**3/day] rhoSi/V [fmol/µm**3/day] Chl a/cell [fmol/#] POC/cell [ng/#] PON/cell [pg/#] bSiO2/cell [fmol/#] rhoC/cell [pmol/#/day] rhoNO3/cell [pmol/#/day] rhoSi/cell [pmol/#/day] Chl a/C [µg/µmol] POC/C [µmol/µmol] PON/C [µmol/µmol] bSiO2/C [µmol/µmol] rhoC/C [µmol/µmol/day] rhoNO3/C [µmol/µmol/day] rhoSi/C [µmol/µmol/day] Temp [°C] Sal [PO4]3- [µmol/kg] Si(OH)4 [µmol/kg] pHT (Potentiometric) pH std dev [±] DIC [µmol/kg] AT [µmol/kg] pHT (Calculated using CO2SYS) pCO2water_SST_wet [µatm] (using CT and TA measurements,...) pCO2water_SST_wet [µatm] (using only the TA and measure...) CSC flag pHT CO2 [µmol/kg] fCO2water_SST_wet [µatm] pCO2water_SST_wet [µatm] [HCO3]- [µmol/kg] [CO3]2- [µmol/kg] Omega Arg Omega Cal laboratory Thalassiosira rotula https://www.marinespecies.org/aphia.php?p=taxdetails&id=148942 urn:lsid:marinespecies.org:taxname:148942 LC-1 500 7.93 0.654 451.7 16.0 21.2 1801.2 5922.5 0.3209 2.11 39.5 6.7 1.132 5.8694 0.0287 0.1683 224.7780 13.6227 2.1984 1.7069 6.1967 0.1253 0.7764 0.788 14.76 2.51 0.423 1.391 5.09 0.82 0.638 4.67 87.38 14.89 2505.25 30.16 4.87 3.78 0.053 1 0.170 0.029 0.345 0.056 0.043 14.60 30.50 18.50 82.90 7.96 0.01 2035.90 2174.50 7.88 597.00 500.00 15 7.88 23.11 594.41 596.57 1917.12 95.67 1.49 2.35 laboratory Thalassiosira rotula https://www.marinespecies.org/aphia.php?p=taxdetails&id=148942 urn:lsid:marinespecies.org:taxname:148942 LC-2 500 7.97 0.906 631.0 14.5 20.3 1595.6 4871.1 0.3419 3.95 52.8 12.6 1.266 4.1710 0.0240 0.1001 160.4627 29.5871 3.5461 3.4452 8.3435 0.1164 0.9715 1.280 17.17 4.12 0.412 1.257 9.63 1.15 1.121 6.26 83.61 20.05 2005.83 46.89 5.62 5.46 0.075 1 0.240 0.024 0.561 0.067 0.065 14.60 30.50 18.50 82.90 7.96 0.01 2035.90 2174.50 7.88 597.00 500.00 15 7.88 23.11 594.41 596.57 1917.12 95.67 1.49 2.35 laboratory Thalassiosira rotula https://www.marinespecies.org/aphia.php?p=taxdetails&id=148942 urn:lsid:marinespecies.org:taxname:148942 LC-3 500 7.99 0.922 1808.0 15.4 20.3 1658.8 5259.0 0.3389 91.9 15.5 2.956 5.9442 0.0322 0.1911 68.0501 9.8276 4.8563 6.9244 0.0714 0.4941 9.67 1.63 0.311 0.986 7.16 1.03 0.511 50.84 8.55 1634.82 37.64 5.44 2.69 1 0.168 0.032 0.740 0.107 0.053 14.60 30.50 18.50 82.90 7.96 0.01 2035.90 2174.50 7.88 597.00 500.00 15 7.88 23.11 594.41 596.57 1917.12 95.67 1.49 2.35 laboratory Thalassiosira rotula https://www.marinespecies.org/aphia.php?p=taxdetails&id=148942 urn:lsid:marinespecies.org:taxname:148942 HC-1 821 7.76 0.824 1059.0 14.9 20.1 1603.3 5003.3 0.3427 5.93 71.6 12.6 1.763 5.6818 0.0246 0.1399 144.9766 41.4556 5.0172 3.1722 8.2627 0.0765 0.6323 1.120 13.51 2.38 0.333 1.038 7.82 0.95 0.599 5.60 67.61 11.90 1664.89 39.15 4.74 3.00 0.083 1 0.176 0.025 0.579 0.070 0.044 14.60 30.50 18.50 79.50 7.75 0.00 2110.00 2177.50 7.66 1045.00 821.00 15 7.66 40.47 1040.83 1044.63 2009.50 60.03 0.94 1.47 laboratory Thalassiosira rotula https://www.marinespecies.org/aphia.php?p=taxdetails&id=148942 urn:lsid:marinespecies.org:taxname:148942 HC-2 821 7.75 0.840 1960.3 14.3 18.6 1400.6 4037.4 0.3629 7.58 91.7 17.5 2.924 5.2511 0.0319 0.1675 145.2718 65.1267 8.0535 5.9923 8.0868 0.0920 0.7441 0.958 11.58 2.21 0.369 1.065 8.23 1.02 0.757 3.87 46.77 8.91 1491.51 33.22 4.11 3.06 0.083 1 0.190 0.032 0.710 0.088 0.065 14.60 30.50 18.50 79.50 7.75 0.00 2110.00 2177.50 7.66 1045.00 821.00 15 7.66 40.47 1040.83 1044.63 2009.50 60.03 0.94 1.47 laboratory Thalassiosira rotula https://www.marinespecies.org/aphia.php?p=taxdetails&id=148942 urn:lsid:marinespecies.org:taxname:148942 HC-3 821 7.75 0.918 2601.0 13.3 19.0 1379.7 3902.3 0.3659 12.30 105.8 25.2 3.540 4.1910 0.0335 0.1403 103.2490 74.0008 9.9596 5.1764 7.4301 0.0700 0.5197 1.210 10.42 2.49 0.349 0.986 7.29 0.98 0.510 4.73 40.66 9.70 1361.03 28.45 3.83 1.99 0.116 1 0.239 0.033 0.700 0.094 0.049 14.60 30.50 18.50 79.50 7.75 0.00 2110.00 2177.50 7.66 1045.00 821.00 15 7.66 40.47 1040.83 1044.63 2009.50 60.03 0.94 1.47