de.pangaea.dataset974253 und dataset PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact 2026-04-30T21:03:38Z https://doi.pangaea.de/10.1594/PANGAEA.974253 <gco:CharacterString>Total bacteria and archaea number with different nematodes and substrates conditions</gco:CharacterString> 2025-01-13T09:17:59 publication https://doi.org/10.1594/PANGAEA.974253 Tamang, Mandip
mandip.tamang@dsmz.de
author Huber, Katharina
katharina.huber@dsmz.de
author Neumann-Schaal, Meina
meina.neumann-schaal@dsmz.de
author Pester, Michael
mip17@dsmz.de
https://orcid.org/0000-0001-6296-4145 information author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital Data contain the quantitative polymerase chain reaction (qPCR) numbers of total bacterial and archaeal 16S ribosomal RNA (16S rRNA) genes from 3 different nematode treatments and two different substrates conditions in the natural soil. In first treatment condition there was no addition of nematode, in second treatment bacterivorous nematode (Acrobeloides buetschlii) was added and in third treatment there was the addition of fungivorous nematode (Aphelenchoides saprophilus). Meanwhile, two different substrate conditions were: with and without maize litter. The natural soil for these experiments were collected from Dikopshof agricultural site (50°48′21″ N, 6°59′9″ E), Bonn, Germany. These qPCR numbers were collected in June and July, 2023 to look at the effect of nematode grazing in the bacterial and archaeal populations. The qPCR was performed using primer set of 1389F (5′-TGYACACACCGCCCGT-3′) and 1492R (5′-GGYTACCTTGTTACGACTT-3′) with 20 µl reactions on a qTOWER3G (Analytik-Jena, Jena, Germany). completed Tamang, Mandip
mandip.tamang@dsmz.de
principalInvestigator Laboratory experiment Nematode grazing quantitative PCR Trophic interactions theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation 6.985833 6.985833 50.805833 50.805833 2021-08-01T00:00:00 2021-08-31T00:00:00 Sample code/label thematicClassification Experimental treatment thematicClassification Substrate Treatment: duration physicalMeasurement days days Treatment: grazers thematicClassification Gene copies per gram sediment physicalMeasurement numbers of total bacterial and archaeal 16S ribosomal RNA (16S rRNA) #/g #/g text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.974253?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: Dikopshof_agricultural_site * LATITUDE: 50.805833 * LONGITUDE: 6.985833 * DATE/TIME START: 2021-08-01T00:00:00 * DATE/TIME END: 2021-08-31T00:00:00 2021-08-01T00:00:00 Supplement to <gco:CharacterString>Succession of Bacteria and Archaea Within the Soil Micro-Food Web Shifts Soil Respiration Dynamics</gco:CharacterString> 2024-01-01 publication https://doi.org/10.1111/1462-2920.70007 Tamang, Mandip
mandip.tamang@dsmz.de
author Sikorski, Johannes https://orcid.org/0000-0002-1890-5540 information author van Bommel, Miriam author Piecha, Marc author Urich, Tim author Ruess, Liliane author Huber, Katharina
katharina.huber@dsmz.de
author Neumann-Schaal, Meina author Pester, Michael
mip17@dsmz.de
https://orcid.org/0000-0001-6296-4145 information author documentDigital Environmental Microbiology 26(11) e70007