10.1594/PANGAEA.974253Tamang, MandipMandipTamangDSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbHHuber, KatharinaKatharinaHuberDSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbHNeumann-Schaal, MeinaMeinaNeumann-SchaalDSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbHBraunschweig Integrated Centre of Systems BiologyPester, MichaelMichaelPester0000-0001-6296-4145DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbHBraunschweig Integrated Centre of Systems BiologyPANGAEA2025Laboratory experimentNematode grazingquantitative PCRTrophic interactionsSample code/labelExperimental treatmentTreatment: durationTreatment: grazersGene copies per gram sediment2021-08-01T00:00:00/2021-08-31T00:00:00Dataset10.1111/1462-2920.70007405 data pointstext/tab-separated-valuesCreative Commons Attribution 4.0 InternationalData contain the quantitative polymerase chain reaction (qPCR) numbers of total bacterial and archaeal 16S ribosomal RNA (16S rRNA) genes from 3 different nematode treatments and two different substrates conditions in the natural soil. In first treatment condition there was no addition of nematode, in second treatment bacterivorous nematode (Acrobeloides buetschlii) was added and in third treatment there was the addition of fungivorous nematode (Aphelenchoides saprophilus). Meanwhile, two different substrate conditions were: with and without maize litter. The natural soil for these experiments were collected from Dikopshof agricultural site (50°48′21″ N, 6°59′9″ E), Bonn, Germany. These qPCR numbers were collected in June and July, 2023 to look at the effect of nematode grazing in the bacterial and archaeal populations. The qPCR was performed using primer set of 1389F (5′-TGYACACACCGCCCGT-3′) and 1492R (5′-GGYTACCTTGTTACGACTT-3′) with 20 µl reactions on a qTOWER3G (Analytik-Jena, Jena, Germany).6.98583350.805833German Research Foundationhttps://doi.org/10.13039/501100001659441899689SPP 2322: Systems ecology of soils – Energy Discharge Modulated by Microbiome and Boundary Conditions (SoilSystems)German Research Foundationhttps://doi.org/10.13039/501100001659HU3067/1-1German Research Foundationhttps://doi.org/10.13039/501100001659NE 2192/4-1German Research Foundationhttps://doi.org/10.13039/501100001659PE2147/6-1German Research Foundationhttps://doi.org/10.13039/501100001659RU 780/22-1German Research Foundationhttps://doi.org/10.13039/501100001659UR198/7-1