Vermandele, Fanny: Sex-specific physiological responses of recovering F2 individuals following parental (F1) exposure to the isolated and combined effect of hypoxia and marine heatwave events in the marine calanoid copepod Acartia tonsa [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.973407 (dataset in review), In: Vermandele, F: Survival and sex-specific physiological responses of the marine copepod Acartia tonsa following parental exposure to the combined effects of hypoxia and a marine heatwave [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.973404 (dataset in review)
Abstract:
Specimens (F1) of the marine calanoid copepod Acartia tonsa were exposed for five days under laboratory conditions to the isolated or combined effects of hypoxia and a marine heatwave. Then, their offspring (F2) were returned to control conditions (C: 18 °C, 100 % O₂ sat.) for the rest of their life cycle to simulate a recovery period. This dataset compiles all morphological and physiological measurements, namely routine metabolic oxygen consumption rates (µmol h⁻¹ and µmol mg⁻¹ h⁻¹), upper thermal limit (°C) as well as length (mm) and dry weight (µg) of recovering F2 adults following parental exposure to the isolated and combined effects of hypoxia and a marine heatwave.
Keyword(s):
Supplement to:
Vermandele, Fanny; Roy, Ellia; Sasaki, Matthew; Winkler, Gesche; Dam, Hans G; Madeira, Diana; Calosi, Piero (in review): You are on your own, kid: parental effects impair an ubiquitous copepod's ability to recover from extreme events.
Related to:
Vermandele, Fanny; Sasaki, Matthew; Winkler, Gesche; Dam, Hans G; Madeira, Diana; Calosi, Piero (2024): Survival, life-history, and physiological responses of the marine calanoid copepod Acartia tonsa under combined hypoxia and marine heatwave events [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.967352
References:
Dam, Hans G; deMayo, James A; Park, Gihong; Norton, Lydia; He, Xuejia; Finiguerra, Michael B; Baumann, Hannes; Brennan, Reid S; Pespeni, Melissa H (2021): Rapid, but limited, zooplankton adaptation to simultaneous warming and acidification. Nature Climate Change, 11(9), 780-786, https://doi.org/10.1038/s41558-021-01131-5
Harada, Alice E; Healy, Timothy M; Burton, Ronald S (2019): Variation in Thermal Tolerance and Its Relationship to Mitochondrial Function Across Populations of Tigriopus californicus. Frontiers in Physiology, 10, 213, https://doi.org/10.3389/fphys.2019.00213
Healy, Timothy M; Bock, Antonia K; Burton, Ronald S (2019): Variation in developmental temperature alters adulthood plasticity of thermal tolerance in Tigriopus californicus. Journal of Experimental Biology, 222 (22), jeb.213405, https://doi.org/10.1242/jeb.213405
Kiørboe, Thomas; Møhlenberg, Flemming; Hamburger, Kirsten (1985): Bioenergetics of the planktonic copepod Acartia tonsa: relation between feeding, egg production and respiration, and composition of specific dynamic action. Marine Ecology Progress Series, 26, 85-97, https://doi.org/10.3354/meps026085
Funding:
Fonds de Recherche du Québec – Nature et Technologies (FRQNT), grant/award no. 274427: FRQNT scholarship
Fundação para a Ciência e Tecnologia (FCT), grant/award no. 2022.00153.CEECIND/CP1720/CT0016: Complex life cycle dynamics under extreme weather events: unravelling the molecular world of marine developmental processes and carryover effects
Fundação para a Ciência e Tecnologia (FCT), grant/award no. CEECIND/01250/2018: Scientific Employment Stimulus researcher contract
Fundação para a Ciência e Tecnologia (FCT), grant/award no. LA/P/0094/2020: Centro de Estudos do Ambiente e do Mar (CESAM)
Fundação para a Ciência e Tecnologia (FCT), grant/award no. UIDB/50017/2020: Centro de Estudos do Ambiente e do Mar (CESAM)
Fundação para a Ciência e Tecnologia (FCT), grant/award no. UIDP/50017/2020: CESAM - Centro de Estudos do Ambiente e do Mar
Natural Sciences and Engineering Research Council (NSERC), grant/award no. RGPIN-2015-06500: Discovery Grants Program - Individual
Natural Sciences and Engineering Research Council (NSERC), grant/award no. RGPIN-2020-05627: Research Support for New Academics (FRQNT)
Coverage:
Latitude: 41.320725 * Longitude: -72.001643
Event(s):
Comment:
The experiment was conducted in the Marine Ecological and Evolutionary Physiology Laboratory (MEEP) at the University of Quebec in Rimouski (UQAR), Rimouski, QC, Canada. Copepods of the species Acartia tonsa were maintained following the stock culture culturing protocol described in Dam et al. 2021 (https://doi.org/10.1038/s41558-021-01131-5). Namely, copepods were kept in three 5 L aquaria filled with artificial seawater kept at a salinity of 28.4 ± 0.5. The physicochemical parameters of the seawater were the following: temperature of 18 ± 0.4 °C, oxygen saturation of 93.7 ± 6.0 %, and pH NBS of 8.20 ± 0.09 before the experiment. The photoperiod was kept at 13h light: 11 h dark. Copepods were fed ad libitum with a mixture of three phytoplankters: Tetraselmis sp., Thalassiosira weissflogii and Rhodomonas salina. Parents (F1) were transferred in 2 L aquaria placed in an experimental system during the 5-d exposure to the isolated and combined effects of hypoxia and MHW conditions. Once the parents (F1) were removed, their offspring (F2) were kept in the same 2 L aquaria and exposed to the control condition to simulate a recovery period until they reached sexual maturity. All traits were measured on F2 adult individuals. For all F1 traits measured during the exposure phase, please refer to the following database:
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Vermandele, Fanny | |||
| 2 | Sampling date/time, experiment | Date/time sampling exp | Vermandele, Fanny | |||
| 3 | Parental treatment | P treat | Vermandele, Fanny | Parental treatment (F1) during the exposure phase. C: Control treatment (18 °C, 100 % O2 sat.) / H: Hypoxia treatment (18 °C, 35 % O2 sat.) / MHW: Marine heatwave treatment (25 °C, 100 % O2 sat.) / HMHW: Combined treatment (25 °C, 35 % O2 sat.) | ||
| 4 | Treatment | Treat | Vermandele, Fanny | Offspring treatment (F2) during the recovery phase. C: Control treatment (18 °C, 100 % O2 sat.) | ||
| 5 | Treatment: temperature | T:temp | °C | Vermandele, Fanny | ||
| 6 | Treatment: Oxygen saturation | T:O2 sat | % | Vermandele, Fanny | ||
| 7 | Number | No | Vermandele, Fanny | System No, refers to the number ID attributed to each shelf holding four aquaria each | ||
| 8 | Aquaria ID | Aquaria ID | Vermandele, Fanny | |||
| 9 | Acartia tonsa, sex | A. tonsa sex | Vermandele, Fanny | F= Females M= Males | ||
| 10 | Acartia tonsa, routine metabolic oxygen consumption | A. tonsa MO2 | µmol/h | Vermandele, Fanny | Respirometry plate, PreSens, SDR SensorDish | Routine metabolic rate per individual |
| 11 | Acartia tonsa, routine metabolic oxygen consumption, specific | A. tonsa spec MO2 | µmol/mg/h | Vermandele, Fanny | Respirometry plate, PreSens, SDR SensorDish | Individual routine metabolic rate divided by individual weight |
| 12 | Acartia tonsa, critical thermal maximum | A. tonsa CTmax | °C | Vermandele, Fanny | CTmax method, modified protocol using the definition of Loss of Locomotor Performance used as endpoint by Harada et al. (2019) and Healy et al. (2019) | |
| 13 | Acartia tonsa, prosome length | A. tonsa prosome l | mm | Vermandele, Fanny | Stereomicroscope, Leica; coupled with Integrated CMOS microscope camera, Leica, IC90 E | Length was measured on pictures using ImageJ |
| 14 | Acartia tonsa, dry mass | A. tonsa dm | µg | Vermandele, Fanny | Calculated according to Kiørboe et al. (1985) | Equation was used to convert prosome lenght to dry weight |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1386 data points