Granse, Dirk; Wendt, Paul; Suchrow, Sigrid; Hanelt, Dieter; Fromm, Jörg; Milin, Morgane; Lima, Oscar; Salmon, Armel; Ainouche, Malika; Jensen, Kai: Cytotype-level responses and of Spartina plants to drought and elevated atmospheric CO₂ concentration [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.973270 (dataset in review)
Abstract:
Global change factors, such as drought and elevated atmospheric CO₂ levels, can have strong impacts on plant performance and evolutionary development. We investigated responses of Spartina (syn.: Sporobolus P.M.Peterson & Saarela) plants on different ploidy levels (cytotypes), i.e., the hybrid Spartina x townsendii H. Groves & J. Groves (syn.: Sporobolus × townsendii P.M.Peterson & Saarela) and its genome-duplicated (allopolyploid) descendant Spartina anglica C.E. Hubbard (syn.: Sporobolus anglicus P.M.Peterson & Saarela), to drought and elevated atmospheric CO₂ levels, regarding plant functional traits and biomass. Spartina plants were sampled in 2017 from a Spartina-marsh at Sönke-Nissen-Koog, Germany, and cytotype analysis was performed by DAPI staining for flow cytometry. Ramets from donor individuals of these plants were used in a greenhouse experiment at University of Hamburg, Germany. The plants were grown in 2 L pots and weekly fertilized with commercial Hakaphos® Blau (15-10-15) NPK fertilizer solution (1% v/v; 50 mL per pot). Three cytotype replicates of both hybrid and allopolyploid plants were treated from May to October 2018 under greenhouse daylight conditions (day/night air temperature 27 °C / 22 °C; 70%–80% relative air humidity; length of the photoperiod typical for the northern Germany region) with drought (vs. well-watered) and elevated (vs. ambient) CO₂ concentrations. At the end of the experiment, total biomass and plant traits, i.e., root-to-shoot ratio, stem diameter, leaf area, stem density, and stomatal length were determined. We here report the result values of three to six technical replicates from three biological replicates per treatment combination.
Supplement to:
Granse, Dirk; Wendt, Paul; Suchrow, Sigrid; Hanelt, Dieter; Fromm, Jörg; Milin, Morgane; Lima, Oscar; Salmon, Armel; Ainouche, Malika; Jensen, Kai (in review): A source of eco-evolutionary responses to global change: The effects of ploidy level on plant functional trait expression in Spartina. Ecology and Evolution
Related to:
Granse, Dirk; Romeiro Motta, Mariana; Suchrow, Sigrid; von Schwartzenberg, Klaus; Schnittger, Arp; Jensen, Kai (2022): The Overlooked Hybrid: Geographic Distribution and Niche Differentiation Between Spartina Cytotypes (Poaceae) in Wadden Sea Salt Marshes. Estuaries and Coasts, 45(5), 1409-1421, https://doi.org/10.1007/s12237-021-00985-4
Granse, Dirk; Wendt, Paul; Suchrow, Sigrid; Hanelt, Dieter; Fromm, Jörg; Milin, Morgane; Lima, Oscar; Salmon, Armel; Ainouche, Malika; Jensen, Kai: Genetic diversity of Spartina populations in Wadden Sea tidal marshes [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.973271
Project(s):
Funding:
Behörde für Wissenschaft, Forschung, Gleichstellung und Bezirke, grant/award no. LFF-FV 36: Hybrids (Landesforschungsförderung Hamburg)
Coverage:
Latitude: 54.600515 * Longitude: 8.877381
Date/Time Start: 2017-07-20T09:00:00 * Date/Time End: 2017-07-27T18:00:00
Minimum Elevation: 1.0 m * Maximum Elevation: 1.0 m
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Granse, Dirk | |||
| 2 | Type of study | Study type | Granse, Dirk | |||
| 3 | Sampling date/time, experiment | Date/time sampling exp | Granse, Dirk | |||
| 4 | Sample number | Sample no | Granse, Dirk | ID of sample | ||
| 5 | Individual code | Individual code | Granse, Dirk | ID of the plant individual (H1, H2, H3, A1, A2, A3), referential link to microsatellite marker dataset (doi:10.1594/PANGAEA.973271) | ||
| 6 | Replicate | Repl | Granse, Dirk | Index of technical replicate | ||
| 7 | Treatment: Carbon dioxide | T:CO2 | Granse, Dirk | Treatment: ambient CO2 (CO2 amb: approximately 400 ppm) or elevated CO2 (CO2 high: 950 ppm) atmospheric concentration | ||
| 8 | Treatment: water | T:H2O | Granse, Dirk | Treatment: well-watered (H2O amb: volumetric soil water content of > 30%) or drought (H2O low: applying 50 mL of fresh water to each plant in a 2 L pot). The volumetric water contents (% v/v) of the pots were monitored weekly using a TDR-meter (Delta-Devices, Cambridge, UK) | ||
| 9 | Cytotype | Cytotype | Granse, Dirk | Flow cytometer, Partec GmbH, PA-II | Cytotype level (hybrid, allopolyploid) determined from flow cytometry (Partec GmbH, Münster, Germany) with a DAPI staining protocol against a standard (Pisum sativum). | |
| 10 | Species, unique identification | Species UID | Granse, Dirk | |||
| 11 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Granse, Dirk | |||
| 12 | Species, unique identification (URI) | Species UID (URI) | Granse, Dirk | |||
| 13 | Species | Species | Granse, Dirk | Optional name of the species | ||
| 14 | Biomass, total | Biom tot | g | Granse, Dirk | Scale | Total biomass at the end of the experiment measured after oven drying at 65 °C for 48 h |
| 15 | Root/shoot ratio | Root/shoot | Granse, Dirk | Calculated | Root-to-shoot ratio calculated from belowground biomass divided by aboveground biomass | |
| 16 | Stem height | Stem h | cm | Granse, Dirk | Ruler stick (RULER) | Height of stem (soil surface to apex) by using a ruler |
| 17 | Stem diameter | Stem diam | mm | Granse, Dirk | Caliper | Diameter of stem at the third leaf. This was measured by using a caliper. |
| 18 | Leaf area | LA | cm2 | Granse, Dirk | Calculated | The width and length were measured using a caliper. Leaf area was calculated from leaf width × leaf length using a fixed coefficient and intercept (leaf_area = 86 + (0.63224 ∗ width ∗ length)). |
| 19 | Stem density, per pot | Stem den | #/# | Granse, Dirk | Counting | Number of stems per pot were counted at the end of the experiment |
| 20 | Stomatal length | Stomatal l | µm | Granse, Dirk | Light microscope, Olympus, BH-2; coupled with Microscope camera, Bresser, MikroCam SP 5.0 | The stomatal length was measured in the third leaf. Leaf sections were incubated for 2 hours in a 1:1 mixture of ethanol and LR-White Resin, then in pure LR-White Resin for 12 hours and embedded in gelatin capsules. Polymerization occurred at 50 °C for 36 hours. The samples were cut into 1 µm thick slices using an ultramicrotome (Ultracut E, Reichert Jung, Vienna), stained with 0.05% toluidine blue in 50 mM MSB, and evaluated under an Olympus BH-2 light microscope with a Bresser MikroCam SP 5.0 camera. Stomatal lengths were measured for six or more stomata per replicate using Bresser CamLabLite 2.0 software. |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2052 data points
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