Tanner, Richelle L; Haworth, Lorna; Nancollas, Sarah; Landa, Susie; Todgham, Anne E: Amphipod glycogen and glucose content under simulated invasion stress in wetlands [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.973189 (dataset in review), In: Tanner, RL et al.: Amphipod protein, glycogen, and glucose content under simulated invasion stress in wetlands [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.973173 (dataset in review)
Abstract:
Every two weeks during the 6-week acclimation period, 8 adults were randomly selected from each tank glycogen analysis (n = 8). Individuals were sacrificed in dry ice and stored at -80 °C. Frozen amphipods were ground into fine powder using a liquid nitrogen cooled mortar and pestle. Glucose and glycogen metabolites were extracted separately to be analyzed using a colorimetric assay. Glycogen was extracted by adding 1 ml of ice-cold 8% HClO₄ to approximately 20 mg of powdered amphipod tissue and then homogenized on ice for approximately 10 seconds with a Pro200 Bio-Gen Series homogenizer (PROScientific, Oxford, CT, USA). A 200 μl sample of the homogenate was frozen at -80 °C for later glycogen analysis. The remaining homogenate slurry was centrifuged at 10,000 g for 10 minutes at 4 °C and the supernatant was extracted and neutralized with 3 mol l⁻¹ K₂CO₃. The solution was centrifuged again at 10,000 g for 10 minutes at 4 °C and frozen at -80 °C for the glucose assay. Glycogen samples were enzymatically digested using previous methods (Hassid & Abraham, 1957) and analyzed for glucose following methodology (Bergmeyer, 1983) modified for microplate spectrophotometer (Synergy HT, Biotek, Winooski, VT, USA). Glycogen content was then corrected for starting glucose.
References:
Tanner, Richelle L; Haworth, Lorna; Nancollas, Sarah; Landa, Susie; Todgham, Anne E (in review): Invasion stress mitigates climate stress in a brackish marsh amphipod. Journal of Applied Ecology
Coverage:
Latitude: 38.117843 * Longitude: -121.983199
Date/Time Start: 2021-07-16T17:00:00 * Date/Time End: 2021-07-16T17:00:00
Event(s):
Comment:
Funding:
* Community function under Phragmites invasion in Suisun Marsh (https://www.seacrlab.com/), Award: Delta Stewardship Council DSF R/SF-107
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Tanner, Richelle L | |||
| 2 | Sampling date/time, experiment | Date/time sampling exp | Tanner, Richelle L | |||
| 3 | Experiment week | Exp week | Tanner, Richelle L | |||
| 4 | Species identification | Species ident | Tanner, Richelle L | Visual identification (VID) | Identified to the genus level | |
| 5 | Species, unique identification | Species UID | Tanner, Richelle L | |||
| 6 | Species, unique identification (URI) | Species UID (URI) | Tanner, Richelle L | |||
| 7 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Tanner, Richelle L | |||
| 8 | Treatment: temperature description | T:Temp descr | Tanner, Richelle L | Controlled using microcontroller board, Uno WIFI, Arduino | P = Phragmites, N = native | |
| 9 | Treatment: light condition | T:light | H = high, L = low; high correlates with native canopy and low correlates with Phragmites canopy | |||
| 10 | Tank number | Tank No | Tanner, Richelle L | Field-collected control individuals are labeled "field" | ||
| 11 | Replicates | Repl | # | Replicate per tank | ||
| 12 | Glycogen | GLY | µmol/g | Microplate Spectrophotometer, TECAN, Spark | Glycogen [µmol glycosyl units/g wet mass] | |
| 13 | Glucose | Glc | µmol/g | Microplate Spectrophotometer, TECAN, Spark | Glucose [µmol glycosyl units/g wet mass] |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2815 data points
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