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Tanner, Richelle L; Haworth, Lorna E; Nancollas, Sarah; Landa, Susie N; Todgham, Anne E (2024): Amphipod glycogen and glucose content under simulated invasion stress in wetlands [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.973189, In: Tanner, RL et al. (2024): Amphipod protein, glycogen, and glucose content under simulated invasion stress in wetlands [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.973173

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Published: 2024-11-21 • DOI registered: 2024-11-21

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Abstract:
Every two weeks during the 6-week acclimation period, 8 adults were randomly selected from each tank glycogen analysis (n = 8). Individuals were sacrificed in dry ice and stored at -80 °C. Frozen amphipods were ground into fine powder using a liquid nitrogen cooled mortar and pestle. Glucose and glycogen metabolites were extracted separately to be analyzed using a colorimetric assay. Glycogen was extracted by adding 1 ml of ice-cold 8% HClO₄ to approximately 20 mg of powdered amphipod tissue and then homogenized on ice for approximately 10 seconds with a Pro200 Bio-Gen Series homogenizer (PROScientific, Oxford, CT, USA). A 200 μl sample of the homogenate was frozen at -80 °C for later glycogen analysis. The remaining homogenate slurry was centrifuged at 10,000 g for 10 minutes at 4 °C and the supernatant was extracted and neutralized with 3 mol l⁻¹ K₂CO₃. The solution was centrifuged again at 10,000 g for 10 minutes at 4 °C and frozen at -80 °C for the glucose assay. Glycogen samples were enzymatically digested using previous methods (Hassid & Abraham, 1957) and analyzed for glucose following methodology (Bergmeyer, 1983) modified for microplate spectrophotometer (Synergy HT, Biotek, Winooski, VT, USA). Glycogen content was then corrected for starting glucose.
Keyword(s):
Amphipoda; invasion ecology; Laboratory experiment; physiology; wetland
Supplement to:
Haworth, Lorna E; Nancollas, Sarah; Landa, Susie N; Todgham, Anne E; Tanner, Richelle L (2024): Invasion stress mitigates climate stress in a brackish marsh amphipod. Journal of Applied Ecology, 1365-2664.14847, https://doi.org/10.1111/1365-2664.14847
References:
Bergmeyer, Hans-Ulrich (1983): Reagents for enzymatic analysis. In: Bergmeyer, H-U (ed.). Methods of enzymatic analysis. 3rd ed. Verlag Chemie, Weinheim, Germany, 126-328
Hassid, W Z; Abraham, S (1957): [7] Chemical procedures for analysis of polysaccharides. In: Sidney P. Colowick, S P, Kaplan, N O (eds.) Methods in Enzymology. Vol. 3. Elsevier, Amsterdam, The Netherlands, 34-50, https://doi.org/10.1016/S0076-6879(57)03345-5
Coverage:
Latitude: 38.117843 * Longitude: -121.983199
Date/Time Start: 2021-07-16T17:00:00 * Date/Time End: 2021-07-16T17:00:00
Event(s):
TuleRed (Origin of Gammarus sp. for the experiments) * Latitude: 38.117843 * Longitude: -121.983199 * Date/Time: 2021-07-16T17:00:00 * Location: Suisun Marsh, California, United States * Method/Device: Sampling by hand (HAND)
Comment:
Funding:
* Community function under Phragmites invasion in Suisun Marsh (https://www.seacrlab.com/), Award: Delta Stewardship Council DSF R/SF-107
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeTanner, Richelle L
2Sampling date/time, experimentDate/time sampling expTanner, Richelle L
3Experiment weekExp weekTanner, Richelle L
4Tank numberTank NoTanner, Richelle LField-collected control individuals are labeled "field"
5Treatment: temperature descriptionT:Temp descrTanner, Richelle LControlled using microcontroller board, Uno WIFI, ArduinoP = Phragmites, N = native
6Treatment: light conditionT:lightH = high, L = low; high correlates with native canopy and low correlates with Phragmites canopy
7Species identificationSpecies identTanner, Richelle LVisual identification (VID)Identified to the genus level
8Species, unique identificationSpecies UIDTanner, Richelle L
9Species, unique identification (URI)Species UID (URI)Tanner, Richelle L
10Species, unique identification (Semantic URI)Species UID (Semantic URI)Tanner, Richelle L
11ReplicateReplTanner, Richelle LReplicate per tank
12Glycogen, per wet massGly/wmµmol/gTanner, Richelle LMicroplate Spectrophotometer, TECAN, SparkGlycogen [µmol glycosyl units/g wet mass]
13Glucose, per wet massGlc/wmµmol/gTanner, Richelle LMicroplate Spectrophotometer, TECAN, SparkGlucose [µmol glycosyl units/g wet mass]
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2794 data points

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