Citation:

PANGAEA.971545

Name: Seawater carbonate chemistry and mortality, growth, and condition of Pacifc cod (Gadus macrocephalus) at early life stages
Published: 2024-08-01
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gadus macrocephalus; Growth/Morphology; Laboratory experiment; Mortality/Survival; Nekton; North Pacific; Pelagos; Reproduction; Single species; Temperate; Temperature

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Abstract:

This study investigated the interactive efects of Ocean Warming and Ocean Acidification on Pacifc cod embryos and larvae by rearing fsh in a factorial experimental design at three temperatures crossed with two CO2 levels. The range of temperatures included cold (3 °C), mid (6 °C) and warm (10 °C) treatments, while an ambient and high (~ 1500 μatm) treatment were used for the CO2 levels. Collectively, these treatments spanned current and future ocean conditions within the Gulf of Alaska and Bering Sea regions of the eastern North Pacifc Ocean. Whole-animal traits, including mortality, growth, and condition, were measured throughout both the embryo and larval life stages.

This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were provided by the author of the related paper (see Related to) to the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-07-31.

Keyword(s):

Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gadus macrocephalus; Growth/Morphology; Laboratory experiment; Mortality/Survival; Nekton; North Pacific; Pelagos; Reproduction; Single species; Temperate; Temperature

Related to:

Slesinger, Emily; Mundorf, Samantha; Laurel, Benjamin J; Hurst, Thomas P (2024): The combined effects of ocean warming and ocean acidification on Pacific cod (Gadus macrocephalus) early life stages. Marine Biology, 171(6), 121, https://doi.org/10.1007/s00227-024-04439-w

References:

Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010

Related code / software:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Slesinger, Emily
2	Species, unique identification	Species UID		Slesinger, Emily
3	Time in days	Time	days	Slesinger, Emily		post hatch
4	Treatment: temperature	T:temp	°C	Slesinger, Emily
5	Treatment	Treat		Slesinger, Emily
6	Identification	ID		Slesinger, Emily		Tank
7	Number	No		Slesinger, Emily
8	Dry mass	Dry m	mg	Slesinger, Emily
9	Dry mass, standard error	Dry m std e	±	Slesinger, Emily
10	Length, standard	I std	mm	Slesinger, Emily
11	Fish, standard length, standard error	Fish SL std e	±	Slesinger, Emily
12	Height	h	mm	Slesinger, Emily		myotome
13	Height, standard error	h std e	±	Slesinger, Emily
14	Condition	Cond		Slesinger, Emily		based on deviance in dry weight and length
15	Condition, standard error	Cond std e	±	Slesinger, Emily		based on deviance in dry weight and length
16	Condition	Cond		Slesinger, Emily		based on deviance in myotome height and length relationship
17	Condition, standard error	Cond std e	±	Slesinger, Emily		based on deviance in myotome height and length relationship
18	Yolk area	Yolk A	mm²	Slesinger, Emily
19	Yolk area, standard error	Yolk A std e	±	Slesinger, Emily
20	Mortality	Mortality		Slesinger, Emily		Egg
21	Mortality	Mortality		Slesinger, Emily		larval
22	pH	pH		Slesinger, Emily	Potentiometric	seawater scale
23	Temperature, water	Temp	°C	Slesinger, Emily
24	Salinity	Sal		Slesinger, Emily
25	Oxygen, dissolved	DO	mg/l	Slesinger, Emily
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Slesinger, Emily
27	Alkalinity, total	AT	µmol/kg	Slesinger, Emily
28	pH	pH		Slesinger, Emily	Calculated with seacarb package in R	seawater scale
29	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Slesinger, Emily
30	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
32	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

3392 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Time [days] (post hatch)	4 T:temp [°C]	5 Treat	6 ID (Tank)	7 No	8 Dry m [mg]	9 Dry m std e [±]	10 I std [mm]	11 Fish SL std e [±]	12 h [mm] (myotome)	13 h std e [±]	14 Cond (based on deviance in dry weig...)	15 Cond std e [±] (based on deviance in dry weig...)	16 Cond (based on deviance in myotome ...)	17 Cond std e [±] (based on deviance in myotome ...)	18 Yolk A [mm²]	19 Yolk A std e [±]	20 Mortality (Egg)	21 Mortality (larval)	22 pH (seawater scale, Potentiometric)	23 Temp [°C]	24 Sal	25 DO [mg/l]	26 DIC [µmol/kg]	27 AT [µmol/kg]	28 pH (seawater scale, Calculated wi...)	29 pCO2water_SST_wet [µatm]	30 CSC flag (Calculated using seacarb afte...)	31 pH (total scale, Calculated using...)	32 CO2 [µmol/kg] (Calculated using seacarb afte...)	33 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	35 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	36 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	37 Omega Arg (Calculated using seacarb afte...)	38 Omega Cal (Calculated using seacarb afte...)
laboratory	Gadus macrocephalus	0	3	ambient	5	14	0.053	0.001	5.432	0.033	0.277	0.005	0.978	0.017	-0.001	0.005	0.010833333	0.001013794			8.10	2.87	30.47	8.81	1937.27	2087.87	8.16	269.55	15	8.16	15.54	268.41	269.55	1812.77	108.96	1.66	2.66
laboratory	Gadus macrocephalus	0	3	ambient	6	16	0.052	0.000	5.449	0.026	0.270	0.004	0.945	0.006	-0.009	0.004	0.008062500	0.001233115			8.23	3.50	29.00	9.21	1855.06	1996.05	8.15	264.30	15	8.16	15.01	263.19	264.30	1738.00	102.04	1.56	2.51
laboratory	Gadus macrocephalus	0	3	ambient	19	16	0.055	0.001	5.265	0.067	0.287	0.003	1.026	0.012	0.015	0.004	0.027062500	0.004457642			7.98	2.80	31.05	9.64	2004.05	2122.17	8.05	364.00	15	8.06	20.95	362.46	364.00	1891.94	91.15	1.39	2.22
laboratory	Gadus macrocephalus	0	3	ambient	20	15	0.053	0.001	5.405	0.034	0.280	0.003	0.969	0.011	0.003	0.004	0.029800000	0.003655654			8.10	3.50	29.00	10.30	1882.38	2018.41	8.13	279.47	15	8.14	15.88	278.30	279.47	1766.78	99.72	1.52	2.45
laboratory	Gadus macrocephalus	0	3	high CO2	13	17	0.050	0.000	5.338	0.043	0.276	0.003	0.932	0.008	0.001	0.003	0.014625000	0.001350540			7.31	3.13	29.77	9.77	2082.84	2047.11	7.51	1344.60	15	7.51	77.01	1338.93	1344.60	1978.93	26.90	0.41	0.66
laboratory	Gadus macrocephalus	0	3	high CO2	14	21	0.053	0.000	5.395	0.033	0.276	0.002	0.966	0.005	-0.002	0.003	0.021195455	0.004662703			7.32	3.05	28.50	9.54	2027.95	1982.61	7.48	1415.57	15	7.49	81.99	1409.58	1415.56	1922.11	23.85	0.36	0.59
laboratory	Gadus macrocephalus	0	3	high CO2	21	15	0.052	0.000	5.326	0.070	0.267	0.005	0.964	0.005	-0.007	0.006	0.009076923	0.001508359			7.29	3.10	31.05	10.12	2176.77	2123.67	7.44	1589.87	15	7.45	90.56	1583.16	1589.87	2061.71	24.49	0.37	0.60
laboratory	Gadus macrocephalus	0	3	high CO2	22	14	0.053	0.000	5.277	0.079	0.267	0.005	0.988	0.009	-0.005	0.004	0.026400000	0.007259673
laboratory	Gadus macrocephalus	0	6	ambient	3	10	0.051	0.001	5.058	0.071	0.274	0.004	0.985	0.027	0.011	0.005	0.049214286	0.011108446			7.93	6.20	31.05	9.02	2012.19	2129.48	7.99	437.65	15	8.00	22.24	435.87	437.65	1897.58	92.36	1.41	2.25
laboratory	Gadus macrocephalus	0	6	ambient	4	15	0.055	0.001	5.217	0.028	0.284	0.002	1.026	0.016	0.015	0.003	0.042666667	0.003900753			8.06	5.50	28.00	9.51	1839.93	1952.86	8.05	337.65	15	8.06	17.94	336.27	337.65	1735.99	86.00	1.32	2.13
laboratory	Gadus macrocephalus	0	6	ambient	17	14	0.056	0.000	5.138	0.030	0.287	0.004	1.058	0.004	0.021	0.005	0.059428571	0.006461819			8.08	6.25	29.25	10.03	1886.78	2023.41	8.09	318.03	15	8.10	16.33	316.74	318.03	1769.47	100.97	1.55	2.48
laboratory	Gadus macrocephalus	0	6	ambient	18	14	0.053	0.000	5.275	0.036	0.283	0.003	0.986	0.004	0.011	0.003	0.041800000	0.005933119			7.93	6.30	30.15	9.24	1982.91	2091.47	7.98	455.47	15	7.99	23.18	453.62	455.47	1872.49	87.24	1.34	2.14
laboratory	Gadus macrocephalus	0	6	high CO2	7	15	0.054	0.000	5.196	0.041	0.285	0.004	1.015	0.006	0.016	0.004	0.075750000	0.015323049			7.32	6.50	28.50	9.61	2010.54	1977.08	7.47	1431.20	15	7.48	73.19	1425.43	1431.20	1911.28	26.07	0.40	0.64
laboratory	Gadus macrocephalus	0	6	high CO2	8	15	0.054	0.000	5.248	0.025	0.283	0.004	1.006	0.007	0.012	0.004	0.061066667	0.007292832			7.30	6.10	31.30	9.05	2196.19	2169.42	7.49	1462.57	15	7.50	74.62	1456.63	1462.57	2089.86	31.71	0.48	0.77
laboratory	Gadus macrocephalus	0	6	high CO2	15	15	0.055	0.000	5.184	0.035	0.277	0.005	1.027	0.008	0.009	0.005	0.074133333	0.007341251			7.28	6.40	30.00	9.31	2104.29	2074.22	7.48	1453.09	15	7.49	73.92	1447.22	1453.09	2001.47	28.91	0.44	0.71
laboratory	Gadus macrocephalus	0	6	high CO2	16	15	0.054	0.000	4.937	0.084	0.271	0.005	1.045	0.005	0.011	0.004	0.065846154	0.008785803			7.28	5.60	28.50	10.05	2039.83	1996.00	7.45	1528.57	15	7.45	80.56	1522.33	1528.57	1935.15	24.12	0.37	0.59
laboratory	Gadus macrocephalus	0	10	ambient	1	11	0.048	0.000	4.901	0.050	0.266	0.004	0.938	0.004	0.009	0.005	0.018937500	0.003280585			8.01	10.00	30.30		1929.63	2039.23	7.93	478.37	15	7.94	21.48	476.54	478.39	1821.40	86.74	1.34	2.12
laboratory	Gadus macrocephalus	0	10	ambient	2	15	0.053	0.000	4.975	0.036	0.271	0.004	1.018	0.005	0.01	0.004	0.015133333	0.004579319			8.10	10.15	29.70	9.21	1893.36	2024.63	8.00	397.28	15	8.01	17.81	395.77	397.30	1776.57	98.97	1.53	2.43
laboratory	Gadus macrocephalus	0	10	ambient	9	12	0.050	0.000	4.707	0.087	0.261	0.005	1.002	0.007	0.008	0.004	0.018583333	0.004142277			8.07	10.30	29.50	9.61	1924.33	2060.62	8.02	394.35	15	8.02	17.61	392.86	394.37	1803.72	102.99	1.59	2.53
laboratory	Gadus macrocephalus	0	10	ambient	10	14	0.051	0.000	4.943	0.045	0.266	0.004	0.994	0.005	0.007	0.004	0.017769231	0.004019430			8.03	10.40	29.10	8.69	1903.10	2035.11	8.01	395.99	15	8.02	17.67	394.50	396.01	1785.35	100.08	1.55	2.47
laboratory	Gadus macrocephalus	0	10	high CO2	11	15	0.049	0.000	4.971	0.042	0.269	0.004	0.941	0.002	0.009	0.004	0.033357143	0.006352820			7.37	10.35	29.65	8.70	2089.80	2043.87	7.37	1902.46	15	7.38	84.79	1895.18	1902.49	1979.17	25.84	0.40	0.63
laboratory	Gadus macrocephalus	0	10	high CO2	12	15	0.050	0.000	4.981	0.052	0.268	0.003	0.977	0.004	0.007	0.003	0.022466667	0.005975240			7.33	10.30	29.50	8.92	2064.70	2024.52	7.39	1805.24	15	7.40	80.63	1798.33	1805.27	1957.23	26.84	0.41	0.66
laboratory	Gadus macrocephalus	0	10	high CO2	23	9	0.050	0.000	4.966	0.042	0.265	0.003	0.975	0.003	0.005	0.003	0.033777778	0.013630825			7.31	9.60	30.00	10.52	2083.93	2066.96	7.49	1459.41	15	7.49	66.53	1453.76	1459.41	1984.47	32.92	0.51	0.81
laboratory	Gadus macrocephalus	0	10	high CO2	24	11	0.050	0.001	4.970	0.065	0.269	0.003	0.973	0.019	0.009	0.003	0.038833333	0.014870060
laboratory	Gadus macrocephalus	11	3	ambient	5	15	0.055	0.001	5.609	0.076	0.272	0.007	0.888	0.027	-0.016	0.005					8.10	2.87	30.47	8.81	1937.27	2087.87	8.16	269.55	15	8.16	15.54	268.41	269.55	1812.77	108.96	1.66	2.66
laboratory	Gadus macrocephalus	11	3	ambient	6	15	0.059	0.002	5.720	0.047	0.272	0.007	0.869	0.030	-0.022	0.005					8.23	3.50	29.00	9.21	1855.06	1996.05	8.15	264.30	15	8.16	15.01	263.19	264.30	1738.00	102.04	1.56	2.51
laboratory	Gadus macrocephalus	11	3	ambient	19	10	0.048	0.002	5.474	0.069	0.262	0.007	0.835	0.014	-0.019	0.006					7.98	2.80	31.05	9.64	2004.05	2122.17	8.05	364.00	15	8.06	20.95	362.46	364.00	1891.94	91.15	1.39	2.22
laboratory	Gadus macrocephalus	11	3	ambient	20	15	0.057	0.002	5.560	0.094	0.275	0.009	0.958	0.020	-0.011	0.005					8.10	3.50	29.00	10.30	1882.38	2018.41	8.13	279.47	15	8.14	15.88	278.30	279.47	1766.78	99.72	1.52	2.45
laboratory	Gadus macrocephalus	11	3	high CO2	13	15	0.069	0.001	5.947	0.059	0.295	0.005	0.862	0.016	-0.013	0.004					7.31	3.13	29.77	9.77	2082.84	2047.11	7.51	1344.60	15	7.51	77.01	1338.93	1344.60	1978.93	26.90	0.41	0.66
laboratory	Gadus macrocephalus	11	3	high CO2	14	15	0.069	0.000	5.882	0.063	0.296	0.005	0.91	0.008	-0.008	0.003					7.32	3.05	28.50	9.54	2027.95	1982.61	7.48	1415.57	15	7.49	81.99	1409.58	1415.56	1922.11	23.85	0.36	0.59
laboratory	Gadus macrocephalus	11	3	high CO2	21	15	0.056	0.001	5.687	0.062	0.267	0.005	0.844	0.007	-0.025	0.003					7.29	3.10	31.05	10.12	2176.77	2123.67	7.44	1589.87	15	7.45	90.56	1583.16	1589.87	2061.71	24.49	0.37	0.60
laboratory	Gadus macrocephalus	11	3	high CO2	22	15	0.055	0.000	5.480	0.096	0.270	0.005	0.947	0.034	-0.013	0.005
laboratory	Gadus macrocephalus	11	6	ambient	3	16	0.066	0.000	5.555	0.075	0.289	0.010	1.106	0.017	0.003	0.006					7.93	6.20	31.05	9.02	2012.19	2129.48	7.99	437.65	15	8.00	22.24	435.87	437.65	1897.58	92.36	1.41	2.25
laboratory	Gadus macrocephalus	11	6	ambient	4	13	0.057	0.002	5.451	0.070	0.273	0.008	1.002	0.035	-0.007	0.005					8.06	5.50	28.00	9.51	1839.93	1952.86	8.05	337.65	15	8.06	17.94	336.27	337.65	1735.99	86.00	1.32	2.13
laboratory	Gadus macrocephalus	11	6	ambient	17	17	0.073	0.003	5.663	0.091	0.292	0.007	1.1	0.019	-0.001	0.004					8.08	6.25	29.25	10.03	1886.78	2023.41	8.09	318.03	15	8.10	16.33	316.74	318.03	1769.47	100.97	1.55	2.48
laboratory	Gadus macrocephalus	11	6	ambient	18	14	0.067	0.002	5.600	0.060	0.285	0.006	1.085	0.034	-0.003	0.005					7.93	6.30	30.15	9.24	1982.91	2091.47	7.98	455.47	15	7.99	23.18	453.62	455.47	1872.49	87.24	1.34	2.14
laboratory	Gadus macrocephalus	11	6	high CO2	7	15	0.075	0.002	5.778	0.113	0.302	0.009	1.054	0.016	0.002	0.005					7.32	6.50	28.50	9.61	2010.54	1977.08	7.47	1431.20	15	7.48	73.19	1425.43	1431.20	1911.28	26.07	0.40	0.64
laboratory	Gadus macrocephalus	11	6	high CO2	8	13	0.072	0.004	5.649	0.112	0.293	0.009	1.086	0.054	0.001	0.005					7.30	6.10	31.30	9.05	2196.19	2169.42	7.49	1462.57	15	7.50	74.62	1456.63	1462.57	2089.86	31.71	0.48	0.77
laboratory	Gadus macrocephalus	11	6	high CO2	15	16	0.065	0.002	5.653	0.098	0.287	0.009	0.996	0.011	-0.005	0.006					7.28	6.40	30.00	9.31	2104.29	2074.22	7.48	1453.09	15	7.49	73.92	1447.22	1453.09	2001.47	28.91	0.44	0.71
laboratory	Gadus macrocephalus	11	6	high CO2	16	18	0.074	0.001	5.638	0.137	0.288	0.009	1.167	0.024	-0.006	0.004					7.28	5.60	28.50	10.05	2039.83	1996.00	7.45	1528.57	15	7.45	80.56	1522.33	1528.57	1935.15	24.12	0.37	0.59
laboratory	Gadus macrocephalus	11	10	ambient	1	15	0.070	0.000	5.549	0.065	0.282	0.009	1.181	0.007	-0.003	0.007					8.01	10.00	30.30		1929.63	2039.23	7.93	478.37	15	7.94	21.48	476.54	478.39	1821.40	86.74	1.34	2.12
laboratory	Gadus macrocephalus	11	10	ambient	2	10	0.064	0.002	5.355	0.088	0.275	0.009	1.181	0.039	-0.001	0.007					8.10	10.15	29.70	9.21	1893.36	2024.63	8.00	397.28	15	8.01	17.81	395.77	397.30	1776.57	98.97	1.53	2.43
laboratory	Gadus macrocephalus	11	10	ambient	9	15	0.069	0.002	5.454	0.102	0.273	0.008	1.216	0.022	-0.008	0.005					8.07	10.30	29.50	9.61	1924.33	2060.62	8.02	394.35	15	8.02	17.61	392.86	394.37	1803.72	102.99	1.59	2.53
laboratory	Gadus macrocephalus	11	10	ambient	10	14	0.069	0.001	5.569	0.072	0.286	0.008	1.141	0.007	0	0.005					8.03	10.40	29.10	8.69	1903.10	2035.11	8.01	395.99	15	8.02	17.67	394.50	396.01	1785.35	100.08	1.55	2.47
laboratory	Gadus macrocephalus	11	10	high CO2	11	15	0.078	0.004	5.714	0.062	0.287	0.009	1.151	0.040	-0.007	0.006					7.37	10.35	29.65	8.70	2089.80	2043.87	7.37	1902.46	15	7.38	84.79	1895.18	1902.49	1979.17	25.84	0.40	0.63
laboratory	Gadus macrocephalus	11	10	high CO2	12	15	0.077	0.002	5.651	0.073	0.283	0.008	1.204	0.016	-0.008	0.005					7.33	10.30	29.50	8.92	2064.70	2024.52	7.39	1805.24	15	7.40	80.63	1798.33	1805.27	1957.23	26.84	0.41	0.66
laboratory	Gadus macrocephalus	11	10	high CO2	23	15	0.076	0.001	5.635	0.073	0.287	0.008	1.204	0.026	-0.003	0.005					7.31	9.60	30.00	10.52	2083.93	2066.96	7.49	1459.41	15	7.49	66.53	1453.76	1459.41	1984.47	32.92	0.51	0.81
laboratory	Gadus macrocephalus	11	10	high CO2	24	15	0.070	0.001	5.632	0.081	0.278	0.009	1.109	0.007	-0.012	0.006
laboratory	Gadus macrocephalus	21	3	ambient	5	13	0.075	0.001	5.902	0.097	0.287	0.008	0.975	0.008	-0.019	0.004					8.10	2.87	30.47	8.81	1937.27	2087.87	8.16	269.55	15	8.16	15.54	268.41	269.55	1812.77	108.96	1.66	2.66
laboratory	Gadus macrocephalus	21	3	ambient	6	15	0.082	0.005	5.903	0.075	0.288	0.008	1.042	0.017	-0.018	0.005					8.23	3.50	29.00	9.21	1855.06	1996.05	8.15	264.30	15	8.16	15.01	263.19	264.30	1738.00	102.04	1.56	2.51
laboratory	Gadus macrocephalus	21	3	ambient	19	10	0.090	0.003	6.063	0.089	0.308	0.009	1.026	0.017	-0.008	0.008					7.98	2.80	31.05	9.64	2004.05	2122.17	8.05	364.00	15	8.06	20.95	362.46	364.00	1891.94	91.15	1.39	2.22
laboratory	Gadus macrocephalus	21	3	ambient	20	14	0.081	0.004	5.871	0.131	0.295	0.010	1.054	0.008	-0.011	0.005					8.10	3.50	29.00	10.30	1882.38	2018.41	8.13	279.47	15	8.14	15.88	278.30	279.47	1766.78	99.72	1.52	2.45
laboratory	Gadus macrocephalus	21	3	high CO2	13	13	0.103	0.001	6.240	0.138	0.322	0.011	1.065	0.053	-0.008	0.004					7.31	3.13	29.77	9.77	2082.84	2047.11	7.51	1344.60	15	7.51	77.01	1338.93	1344.60	1978.93	26.90	0.41	0.66
laboratory	Gadus macrocephalus	21	3	high CO2	14	9	0.105	0.001	6.455	0.039	0.335	0.003	0.921	0.010	-0.008	0.004					7.32	3.05	28.50	9.54	2027.95	1982.61	7.48	1415.57	15	7.49	81.99	1409.58	1415.56	1922.11	23.85	0.36	0.59
laboratory	Gadus macrocephalus	21	3	high CO2	21	10	0.110	0.001	6.520	0.112	0.327	0.010	0.92	0.012	-0.023	0.003					7.29	3.10	31.05	10.12	2176.77	2123.67	7.44	1589.87	15	7.45	90.56	1583.16	1589.87	2061.71	24.49	0.37	0.60
laboratory	Gadus macrocephalus	21	6	ambient	3	15	0.137	0.002	6.581	0.122	0.380	0.014	1.103	0.018	0.024	0.007					7.93	6.20	31.05	9.02	2012.19	2129.48	7.99	437.65	15	8.00	22.24	435.87	437.65	1897.58	92.36	1.41	2.25
laboratory	Gadus macrocephalus	21	6	ambient	4	15	0.148	0.007	6.603	0.159	0.383	0.017	1.173	0.030	0.023	0.008					8.06	5.50	28.00	9.51	1839.93	1952.86	8.05	337.65	15	8.06	17.94	336.27	337.65	1735.99	86.00	1.32	2.13
laboratory	Gadus macrocephalus	21	6	ambient	17	15	0.139	0.005	6.610	0.108	0.370	0.010	1.09	0.008	0.012	0.003					8.08	6.25	29.25	10.03	1886.78	2023.41	8.09	318.03	15	8.10	16.33	316.74	318.03	1769.47	100.97	1.55	2.48
laboratory	Gadus macrocephalus	21	6	ambient	18	15	0.161	0.003	6.897	0.085	0.414	0.011	1.055	0.006	0.032	0.006					7.93	6.30	30.15	9.24	1982.91	2091.47	7.98	455.47	15	7.99	23.18	453.62	455.47	1872.49	87.24	1.34	2.14
laboratory	Gadus macrocephalus	21	6	high CO2	7	15	0.137	0.006	6.556	0.122	0.374	0.011	1.114	0.009	0.02	0.005					7.32	6.50	28.50	9.61	2010.54	1977.08	7.47	1431.20	15	7.48	73.19	1425.43	1431.20	1911.28	26.07	0.40	0.64
laboratory	Gadus macrocephalus	21	6	high CO2	8	15	0.152	0.003	6.723	0.123	0.388	0.013	1.115	0.010	0.02	0.007					7.30	6.10	31.30	9.05	2196.19	2169.42	7.49	1462.57	15	7.50	74.62	1456.63	1462.57	2089.86	31.71	0.48	0.77
laboratory	Gadus macrocephalus	21	6	high CO2	15	15	0.156	0.002	6.835	0.080	0.392	0.009	1.064	0.007	0.016	0.005					7.28	6.40	30.00	9.31	2104.29	2074.22	7.48	1453.09	15	7.49	73.92	1447.22	1453.09	2001.47	28.91	0.44	0.71
laboratory	Gadus macrocephalus	21	6	high CO2	16	15	0.144	0.002	6.780	0.091	0.377	0.010	1.021	0.006	0.005	0.004					7.28	5.60	28.50	10.05	2039.83	1996.00	7.45	1528.57	15	7.45	80.56	1522.33	1528.57	1935.15	24.12	0.37	0.59
laboratory	Gadus macrocephalus	28	3	ambient	5	2	0.095	0.000	6.440	0.510	0.306	0.039	0.841	0.000	-0.04	0.001					8.10	2.87	30.47	8.81	1937.27	2087.87	8.16	269.55	15	8.16	15.54	268.41	269.55	1812.77	108.96	1.66	2.66
laboratory	Gadus macrocephalus	28	3	ambient	19	15	0.124	0.003	6.621	0.100	0.349	0.009	0.969	0.012	-0.009	0.005					8.23	3.50	29.00	9.21	1855.06	1996.05	8.15	264.30	15	8.16	15.01	263.19	264.30	1738.00	102.04	1.56	2.51
laboratory	Gadus macrocephalus	28	3	ambient	20	15	0.100	0.003	6.484	0.098	0.336	0.011	0.861	0.011	-0.011	0.008					7.98	2.80	31.05	9.64	2004.05	2122.17	8.05	364.00	15	8.06	20.95	362.46	364.00	1891.94	91.15	1.39	2.22
laboratory	Gadus macrocephalus	28	3	high CO2	13	10	0.145	0.002	6.615	0.207	0.352	0.015	1.143	0.028	-0.009	0.007					7.31	3.13	29.77	9.77	2082.84	2047.11	7.51	1344.60	15	7.51	77.01	1338.93	1344.60	1978.93	26.90	0.41	0.66
laboratory	Gadus macrocephalus	28	3	high CO2	14	15	0.120	0.004	6.576	0.137	0.344	0.009	0.97	0.031	-0.012	0.006					7.32	3.05	28.50	9.54	2027.95	1982.61	7.48	1415.57	15	7.49	81.99	1409.58	1415.56	1922.11	23.85	0.36	0.59
laboratory	Gadus macrocephalus	28	3	high CO2	21	15	0.146	0.002	6.807	0.161	0.363	0.010	1.017	0.010	-0.015	0.007					7.29	3.10	31.05	10.12	2176.77	2123.67	7.44	1589.87	15	7.45	90.56	1583.16	1589.87	2061.71	24.49	0.37	0.60
laboratory	Gadus macrocephalus	28	3	high CO2	22	9	0.124	0.004	6.470	0.209	0.337	0.017	1.075	0.010	-0.012	0.010
laboratory	Gadus macrocephalus	28	6	ambient	3	15	0.199	0.005	7.121	0.165	0.424	0.019	1.137	0.009	0.017	0.006					7.93	6.20	31.05	9.02	2012.19	2129.48	7.99	437.65	15	8.00	22.24	435.87	437.65	1897.58	92.36	1.41	2.25
laboratory	Gadus macrocephalus	28	6	ambient	4	15	0.210	0.006	7.241	0.145	0.439	0.015	1.119	0.016	0.02	0.007					8.06	5.50	28.00	9.51	1839.93	1952.86	8.05	337.65	15	8.06	17.94	336.27	337.65	1735.99	86.00	1.32	2.13
laboratory	Gadus macrocephalus	28	6	ambient	17	14	0.218	0.007	7.463	0.165	0.455	0.018	1.017	0.019	0.013	0.005					8.08	6.25	29.25	10.03	1886.78	2023.41	8.09	318.03	15	8.10	16.33	316.74	318.03	1769.47	100.97	1.55	2.48
laboratory	Gadus macrocephalus	28	6	ambient	18	7	0.242	0.013	7.468	0.337	0.448	0.039	1.118	0.007	0	0.011					7.93	6.30	30.15	9.24	1982.91	2091.47	7.98	455.47	15	7.99	23.18	453.62	455.47	1872.49	87.24	1.34	2.14
laboratory	Gadus macrocephalus	28	6	high CO2	7	15	0.254	0.004	7.585	0.167	0.471	0.020	1.102	0.011	0.014	0.006					7.32	6.50	28.50	9.61	2010.54	1977.08	7.47	1431.20	15	7.48	73.19	1425.43	1431.20	1911.28	26.07	0.40	0.64
laboratory	Gadus macrocephalus	28	6	high CO2	8	14	0.225	0.008	7.494	0.178	0.442	0.019	1.03	0.025	-0.005	0.006					7.30	6.10	31.30	9.05	2196.19	2169.42	7.49	1462.57	15	7.50	74.62	1456.63	1462.57	2089.86	31.71	0.48	0.77
laboratory	Gadus macrocephalus	28	6	high CO2	15	15	0.227	0.004	7.364	0.179	0.437	0.020	1.123	0.016	0.004	0.005					7.28	6.40	30.00	9.31	2104.29	2074.22	7.48	1453.09	15	7.49	73.92	1447.22	1453.09	2001.47	28.91	0.44	0.71
laboratory	Gadus macrocephalus	28	6	high CO2	16	14	0.204	0.004	7.130	0.138	0.406	0.016	1.164	0.036	0	0.011					7.28	5.60	28.50	10.05	2039.83	1996.00	7.45	1528.57	15	7.45	80.56	1522.33	1528.57	1935.15	24.12	0.37	0.59
laboratory	Gadus macrocephalus	36	6	ambient	3	15	0.353	0.008	8.236	0.183	0.538	0.020	1.081	0.026	0.001	0.010					7.93	6.20	31.05	9.02	2012.19	2129.48	7.99	437.65	15	8.00	22.24	435.87	437.65	1897.58	92.36	1.41	2.25
laboratory	Gadus macrocephalus	36	6	ambient	4	6	0.262	0.008	7.691	0.241	0.474	0.036	1.072	0.002	0.007	0.011					8.06	5.50	28.00	9.51	1839.93	1952.86	8.05	337.65	15	8.06	17.94	336.27	337.65	1735.99	86.00	1.32	2.13
laboratory	Gadus macrocephalus	36	6	ambient	17	15	0.353	0.002	8.342	0.093	0.550	0.012	1.018	0.007	0.004	0.006					8.08	6.25	29.25	10.03	1886.78	2023.41	8.09	318.03	15	8.10	16.33	316.74	318.03	1769.47	100.97	1.55	2.48
laboratory	Gadus macrocephalus	36	6	high CO2	7	15	0.396	0.008	8.622	0.108	0.568	0.016	0.988	0.011	-0.017	0.006					7.32	6.50	28.50	9.61	2010.54	1977.08	7.47	1431.20	15	7.48	73.19	1425.43	1431.20	1911.28	26.07	0.40	0.64
laboratory	Gadus macrocephalus	36	6	high CO2	8	15	0.349	0.004	8.238	0.146	0.533	0.021	1.064	0.016	-0.001	0.007					7.30	6.10	31.30	9.05	2196.19	2169.42	7.49	1462.57	15	7.50	74.62	1456.63	1462.57	2089.86	31.71	0.48	0.77
laboratory	Gadus macrocephalus	36	6	high CO2	15	15	0.342	0.009	8.328	0.167	0.530	0.020	0.99	0.006	-0.018	0.007					7.28	6.40	30.00	9.31	2104.29	2074.22	7.48	1453.09	15	7.49	73.92	1447.22	1453.09	2001.47	28.91	0.44	0.71
laboratory	Gadus macrocephalus	36	6	high CO2	16	15	0.289	0.002	7.899	0.190	0.480	0.026	1.059	0.019	-0.015	0.006					7.28	5.60	28.50	10.05	2039.83	1996.00	7.45	1528.57	15	7.45	80.56	1522.33	1528.57	1935.15	24.12	0.37	0.59
laboratory	Gadus macrocephalus		3	ambient	5														0.156	0.184	8.10	2.87	30.47	8.81	1937.27	2087.87	8.16	269.55	15	8.16	15.54	268.41	269.55	1812.77	108.96	1.66	2.66
laboratory	Gadus macrocephalus		3	ambient	6														0.195	0.192	8.23	3.50	29.00	9.21	1855.06	1996.05	8.15	264.30	15	8.16	15.01	263.19	264.30	1738.00	102.04	1.56	2.51
laboratory	Gadus macrocephalus		3	ambient	19														0.125	0.052	7.98	2.80	31.05	9.64	2004.05	2122.17	8.05	364.00	15	8.06	20.95	362.46	364.00	1891.94	91.15	1.39	2.22
laboratory	Gadus macrocephalus		3	ambient	20														0.207	0.051	8.10	3.50	29.00	10.30	1882.38	2018.41	8.13	279.47	15	8.14	15.88	278.30	279.47	1766.78	99.72	1.52	2.45
laboratory	Gadus macrocephalus		3	high CO2	13														0.112	0.082	7.31	3.13	29.77	9.77	2082.84	2047.11	7.51	1344.60	15	7.51	77.01	1338.93	1344.60	1978.93	26.90	0.41	0.66
laboratory	Gadus macrocephalus		3	high CO2	14														0.104	0.083	7.32	3.05	28.50	9.54	2027.95	1982.61	7.48	1415.57	15	7.49	81.99	1409.58	1415.56	1922.11	23.85	0.36	0.59
laboratory	Gadus macrocephalus		3	high CO2	21														0.106	0.071	7.29	3.10	31.05	10.12	2176.77	2123.67	7.44	1589.87	15	7.45	90.56	1583.16	1589.87	2061.71	24.49	0.37	0.60
laboratory	Gadus macrocephalus		3	high CO2	22														0.136	0.057
laboratory	Gadus macrocephalus		6	ambient	3														0.211	0.041	7.93	6.20	31.05	9.02	2012.19	2129.48	7.99	437.65	15	8.00	22.24	435.87	437.65	1897.58	92.36	1.41	2.25
laboratory	Gadus macrocephalus		6	ambient	4														0.265	0.108	8.06	5.50	28.00	9.51	1839.93	1952.86	8.05	337.65	15	8.06	17.94	336.27	337.65	1735.99	86.00	1.32	2.13
laboratory	Gadus macrocephalus		6	ambient	17														0.144	0.045	8.08	6.25	29.25	10.03	1886.78	2023.41	8.09	318.03	15	8.10	16.33	316.74	318.03	1769.47	100.97	1.55	2.48
laboratory	Gadus macrocephalus		6	ambient	18														0.184	0.194	7.93	6.30	30.15	9.24	1982.91	2091.47	7.98	455.47	15	7.99	23.18	453.62	455.47	1872.49	87.24	1.34	2.14
laboratory	Gadus macrocephalus		6	high CO2	7														0.152	0.028	7.32	6.50	28.50	9.61	2010.54	1977.08	7.47	1431.20	15	7.48	73.19	1425.43	1431.20	1911.28	26.07	0.40	0.64
laboratory	Gadus macrocephalus		6	high CO2	8														0.142	0.056	7.30	6.10	31.30	9.05	2196.19	2169.42	7.49	1462.57	15	7.50	74.62	1456.63	1462.57	2089.86	31.71	0.48	0.77
laboratory	Gadus macrocephalus		6	high CO2	15														0.170	0.053	7.28	6.40	30.00	9.31	2104.29	2074.22	7.48	1453.09	15	7.49	73.92	1447.22	1453.09	2001.47	28.91	0.44	0.71
laboratory	Gadus macrocephalus		6	high CO2	16														0.281	0.075	7.28	5.60	28.50	10.05	2039.83	1996.00	7.45	1528.57	15	7.45	80.56	1522.33	1528.57	1935.15	24.12	0.37	0.59
laboratory	Gadus macrocephalus		10	ambient	1														0.263	0.165	8.01	10.00	30.30		1929.63	2039.23	7.93	478.37	15	7.94	21.48	476.54	478.39	1821.40	86.74	1.34	2.12
laboratory	Gadus macrocephalus		10	ambient	2														0.420	0.232	8.10	10.15	29.70	9.21	1893.36	2024.63	8.00	397.28	15	8.01	17.81	395.77	397.30	1776.57	98.97	1.53	2.43
laboratory	Gadus macrocephalus		10	ambient	9														0.311	0.254	8.07	10.30	29.50	9.61	1924.33	2060.62	8.02	394.35	15	8.02	17.61	392.86	394.37	1803.72	102.99	1.59	2.53
laboratory	Gadus macrocephalus		10	ambient	10														0.382	0.291	8.03	10.40	29.10	8.69	1903.10	2035.11	8.01	395.99	15	8.02	17.67	394.50	396.01	1785.35	100.08	1.55	2.47
laboratory	Gadus macrocephalus		10	high CO2	11														0.298	0.215	7.37	10.35	29.65	8.70	2089.80	2043.87	7.37	1902.46	15	7.38	84.79	1895.18	1902.49	1979.17	25.84	0.40	0.63
laboratory	Gadus macrocephalus		10	high CO2	12														0.278	0.226	7.33	10.30	29.50	8.92	2064.70	2024.52	7.39	1805.24	15	7.40	80.63	1798.33	1805.27	1957.23	26.84	0.41	0.66
laboratory	Gadus macrocephalus		10	high CO2	23														0.324	0.294	7.31	9.60	30.00	10.52	2083.93	2066.96	7.49	1459.41	15	7.49	66.53	1453.76	1459.41	1984.47	32.92	0.51	0.81
laboratory	Gadus macrocephalus		10	high CO2	24														0.296	0.281