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Stratmann, Tanja; Miranda Montenegro, Mario Luis; de Kluijver, Anna; Busch, Kathrin; Kelly, Michelle; Mills, Sadie; Schupp, Peter (2024): Bacterial composition of shallow-water and deep-water demo- and hexactinellid sponges from New Zealand collected during SONNE cruise SO254 [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.971514 (DOI registration in progress)

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Abstract:
During R/V Sonne cruise SO254, we conducted ex-situ experiments with shallow- and deep-water demo- and hexactinellid sponges from New Zealand. Over a period of 5 hours, the sponges were incubated at in-situ temperature in the light (shallow-water incubation) or in the dark (deep-water incubation), and water samples for dissolved organic carbon (DOC), total dissolved nitrogen (TDN), and fluorescing dissolved organic matter (FDOM) were taken every hour. At the end of the incubations, samples for (biogeochemical) tissue analyses (including fatty acid analyses) were taken of the sponges together with samples for microbial analyses of the bacterial community living inside the sponges. Bacteria community composition of 4 species (number of replicates per species: 1) were analyzed based on DNA extracted from sub-samples taken from the sponges at the end of the incubations. After sequencing, representative amplicon sequence variants (ASVs) were classified taxonomically with the SILVA database.
Keyword(s):
EEM; long-chain fatty acid; Pacific; PARAFAC; PLFA; PUFA; sponge
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03G0254A: SO254 PoriBacNewZ
Coverage:
Median Latitude: -34.884040 * Median Longitude: 179.763267 * South-bound Latitude: -39.990600 * West-bound Longitude: 178.215370 * North-bound Latitude: -29.283300 * East-bound Longitude: -177.900000
Date/Time Start: 2017-02-03T00:00:00 * Date/Time End: 2017-02-08T19:28:00
Minimum Elevation: -1448.0 m * Maximum Elevation: -6.0 m
Event(s):
SO254_33-1 * Latitude: -35.378220 * Longitude: 178.974430 * Date/Time: 2017-02-06T19:56:00 * Elevation: -1448.0 m * Location: South Pacific Ocean * Campaign: SO254 (Pori Bac NewZ) * Basis: Sonne_2 * Method/Device: Remote operated vehicle (ROV)
SO254_36-1 * Latitude: -39.990600 * Longitude: 178.215370 * Date/Time: 2017-02-08T19:28:00 * Elevation: -828.0 m * Location: South Pacific Ocean * Campaign: SO254 (Pori Bac NewZ) * Basis: Sonne_2 * Method/Device: Remote operated vehicle (ROV)
SO254_diver * Latitude: -29.283300 * Longitude: -177.900000 * Date/Time: 2017-02-03T00:00:00 * Elevation: -6.0 m * Campaign: SO254 (Pori Bac NewZ) * Basis: Sonne_2 * Method/Device: Sampling by diver (DIVER)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventStratmann, Tanja
2Campaign of eventCampaignStratmann, Tanja
3Date/Time of eventDate/TimeStratmann, Tanja
4Latitude of eventLatitudeStratmann, Tanja
5Longitude of eventLongitudeStratmann, Tanja
6Elevation of eventElevationmStratmann, Tanja
7Sample code/labelSample labelStratmann, Tanja
8Area/localityAreaStratmann, Tanja
9Taxon/taxaTaxaStratmann, Tanja
10Taxon/taxa, unique identificationTaxa UIDStratmann, Tanja
11Taxon/taxa, unique identification (Semantic URI)Taxa UID (Semantic URI)Stratmann, Tanja
12Taxon/taxa, unique identification (URI)Taxa UID (URI)Stratmann, Tanja
13Laboratory code/labelLab labelStratmann, Tanja
14AcidobacteriaAcidobacteria%Stratmann, Tanja
15ActinobacteriaActinobacteria%Stratmann, Tanja
16Bacteria, phylaBac phyla%Stratmann, TanjaAncK6
17BacteroidetesBacteroidetes%Stratmann, Tanja
18ChlamydiaeChlamydiae%Stratmann, Tanja
19ChloroflexotaChloroflexota%Stratmann, Tanja
20CyanobacteriaCyanobacteria%Stratmann, Tanja
21DadabacteriaDadabacteria%Stratmann, Tanja
22Deinococcus-ThermusDein-Therm%Stratmann, Tanja
23EntotheonellaeotaEntotheonellaeota%Stratmann, Tanja
24EuryarchaeotaEuryarchaeota%Stratmann, Tanja
25FibrobacteresFibrobacteres%Stratmann, Tanja
26FirmicutesFirmicutes%Stratmann, Tanja
27GemmatimonadetesGemmatimonadetes%Stratmann, Tanja
28MargulisbacteriaMargulisbacteria%Stratmann, Tanja
29MarinimicrobiaMarinimicrobia%Stratmann, Tanja
30NitrospinaeNitrospinae%Stratmann, Tanja
31NitrospiraeNitrospirae%Stratmann, Tanja
32PatescibacteriaPatescibacteria%Stratmann, Tanja
33Bacteria, phylaBac phyla%Stratmann, TanjaPAUC34f
34PlanctomycetesPlanctomycetes%Stratmann, Tanja
35PoribacteriaPoribacteria%Stratmann, Tanja
36ProteobacteriaProteobacteria%Stratmann, Tanja
37SpirochaetesSpirochaetes%Stratmann, Tanja
38TenericutesTenericutes%Stratmann, Tanja
39VerrucomicrobiaVerrucomicrobia%Stratmann, Tanja
40Bacteria, phylaBac phyla%Stratmann, TanjaUnclassified
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
128 data points

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