Citation:

PANGAEA.971360

Name: Seawater carbonate chemistry and uptake of dissolved inorganic nitrogen and N2 fixation by Crocosphaera watsonii
Published: 2024-07-24
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bacteria; Biomass/Abundance/Elemental composition; Containers and aquaria (20-1000 L or < 1 m**2); Crocosphaera watsonii; Cyanobacteria; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature

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Abstract:

We conducted a minicosm experiment with pre-acclimated cultures of the globally significant diazotroph Crocosphaera watsonii strain WH8501 ("Crocosphaera"). PH and temperature were altered simultaneously to match the RCP scenarios 4.5 and 6 and investigate a more realistic future scenario compared to studies that focus on changes of a single stressor only. The cell abundance and nitrogen metabolism of Crocosphaera was monitored over 5 days.

This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were extracted from tables in the related paper (see Related to) by the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-07-23.

Keyword(s):

Bacteria; Biomass/Abundance/Elemental composition; Containers and aquaria (20-1000 L or < 1 m**2); Crocosphaera watsonii; Cyanobacteria; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature

Related to:

Umbricht, Jacqueline; Filella, Alba; Klett, Angelina; Vogts, Angela; Benavides, Mar; Voss, Maren (2024): Uptake of dissolved inorganic nitrogen and N2 fixation by Crocosphaera watsonii under climate change scenarios. Frontiers in Marine Science, 11, 1388214, https://doi.org/10.3389/fmars.2024.1388214

References:

Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010

Related code / software:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html

Further details:

Filella, Alba; Umbricht, Jacqueline; Vogts, Angela; Klett, Angelina; Vannier, Thomas; Grosso, Olivier; Voss, Maren; Riemann, Lasse; Benavides, Mar (2023): DOM offsets the detrimental effects of climate change in the nitrogen fixing cyanobacterium Crocosphaera [dataset]. figshare, https://doi.org/10.6084/M9.FIGSHARE.22773503.V2

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Voss, Maren
2	Species	Species		Voss, Maren
3	Date	Date		Voss, Maren
4	Day of experiment	DOE	day	Voss, Maren
5	Treatment	Treat		Voss, Maren
6	Identification	ID		Voss, Maren		tank
7	pH	pH		Voss, Maren		spectro photometry
8	pH	pH		Voss, Maren		daily mean IKS
9	pH, standard deviation	pH std dev	±	Voss, Maren		daily mean IKS
10	Temperature, water	Temp	°C	Voss, Maren		daily mean IKS
11	Temperature, water, standard deviation	Temp std dev	±	Voss, Maren		daily mean IKS
12	Cell density	Cells	#/ml	Voss, Maren		Crocosphaera
13	Nitrite	[NO2]-	µmol/l	Voss, Maren
14	Nitrate	[NO3]-	µmol/l	Voss, Maren
15	Ammonium	[NH4]+	µmol/l	Voss, Maren
16	Phosphate	[PO4]3-	µmol/l	Voss, Maren
17	Nitrogen, organic, dissolved	DON	µmol/l	Voss, Maren
18	Phosphorus, organic, dissolved	DOP	µmol/l	Voss, Maren
19	Carbon, organic, dissolved	DOC	µmol/l	Voss, Maren
20	Bacteria	Bact	#/ml	Voss, Maren
21	Nitrogen, organic, particulate	PON	µmol/l	Voss, Maren		natural abundance
22	Carbon, organic, particulate	POC	µmol/l	Voss, Maren		natural abundance
23	Carbon, organic, particulate/Nitrogen, organic, particulate ratio	POC/PON		Voss, Maren
24	δ15N	δ15N	‰	Voss, Maren		natural abundance
25	δ13C	δ13C	‰	Voss, Maren		natural abundance
26	Nitrogen fixation rate	N2 fix	nmol/l/h	Voss, Maren		bulk
27	Nitrogen fixation rate	N2 fix	nmol/l/day	Voss, Maren		daily bulk
28	Carbon fixation rate	C fix	µmol/l/h	Voss, Maren		bulk
29	Carbon fixation rate	C fix	µmol/l/day	Voss, Maren		daily bulk
30	Carbon fixation rate	C fix	µmol/l/day	Voss, Maren		mean daily bulk
31	Carbon fixation rate, standard deviation	C fix std dev	±	Voss, Maren
32	Nitrogen fixation rate, per cell	N2 fix/cell	fmol/#/day	Voss, Maren
33	Nitrate uptake rate per cell	NO3 upt rate/cell	fmol/#/day	Voss, Maren
34	Nitrate uptake rate, standard deviation	NO3 upt rate std dev	±	Voss, Maren
35	Ammonium uptake rate per cell	NH4+ upt rate/cell	fmol/#/day	Voss, Maren
36	Ammonium uptake rate, standard deviation	NH4+ upt rate/cell std dev	±	Voss, Maren
37	Alkalinity, total	AT	µmol/kg	Voss, Maren
38	pH	pH		Voss, Maren	Spectrophotometric	total scale, insi
39	Temperature, water	Temp	°C	Voss, Maren		insi
40	Salinity	Sal		Voss, Maren
41	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	adult
43	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	adult
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	adult
45	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	adult
46	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	adult
47	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	adult
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	adult
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	adult

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

2239 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species	3 Date	4 DOE [day]	5 Treat	6 ID (tank)	7 pH (spectro photometry)	8 pH (daily mean IKS)	9 pH std dev [±] (daily mean IKS)	10 Temp [°C] (daily mean IKS)	11 Temp std dev [±] (daily mean IKS)	12 Cells [#/ml] (Crocosphaera)	13 [NO2]- [µmol/l]	14 [NO3]- [µmol/l]	15 [NH4]+ [µmol/l]	16 [PO4]3- [µmol/l]	17 DON [µmol/l]	18 DOP [µmol/l]	19 DOC [µmol/l]	20 Bact [#/ml]	21 PON [µmol/l] (natural abundance)	22 POC [µmol/l] (natural abundance)	23 POC/PON	24 δ15N [‰] (natural abundance)	25 δ13C [‰] (natural abundance)	26 N2 fix [nmol/l/h] (bulk)	27 N2 fix [nmol/l/day] (daily bulk)	28 C fix [µmol/l/h] (bulk)	29 C fix [µmol/l/day] (daily bulk)	30 C fix [µmol/l/day] (mean daily bulk)	31 C fix std dev [±]	32 N2 fix/cell [fmol/#/day]	33 NO3 upt rate/cell [fmol/#/day]	34 NO3 upt rate std dev [±]	35 NH4+ upt rate/cell [fmol/#/day]	36 NH4+ upt rate/cell std dev [±]	37 AT [µmol/kg]	38 pH (total scale, insi, Spectropho...)	39 Temp [°C] (insi)	40 Sal	41 CSC flag (Calculated using seacarb afte...)	42 CO2 [µmol/kg] (adult, Calculated using seaca...)	43 fCO2water_SST_wet [µatm] (adult, Calculated using seaca...)	44 pCO2water_SST_wet [µatm] (adult, Calculated using seaca...)	45 [HCO3]- [µmol/kg] (adult, Calculated using seaca...)	46 [CO3]2- [µmol/kg] (adult, Calculated using seaca...)	47 DIC [µmol/kg] (adult, Calculated using seaca...)	48 Omega Arg (adult, Calculated using seaca...)	49 Omega Cal (adult, Calculated using seaca...)
laboratory	Crocosphaera watsonii	2021-09-10	-2	control	a		8.07	0.04	26.03	0.08	8296	0.0200	0.160		4.37	6.81	0.16	295	1730000
laboratory	Crocosphaera watsonii	2021-09-10	-2	control	b		8.06	0.02	26.05	0.19	12480	0.0090	0.080		4.45	6.79	0.05	269	1860000
laboratory	Crocosphaera watsonii	2021-09-10	-2	intermediate	a		8.03	0.02	26.86	1.06	22760	0.0105	0.120		4.48	6.63	0.07	265	1250000
laboratory	Crocosphaera watsonii	2021-09-10	-2	intermediate	b		8.03	0.01	28.04	0.08	9128	0.0105	0.080		4.38	7.18	0.13	405	1270000
laboratory	Crocosphaera watsonii	2021-09-10	-2	intermediate	c		7.79	0.40	28.12	0.32	7492	0.0095	0.090		4.48	9.22	0.25	317	1220000
laboratory	Crocosphaera watsonii	2021-09-10	-2	extreme	a		7.92	0.39	29.88	1.51	8836	0.0135	0.110		4.29	7.38	0.12	337	1080000
laboratory	Crocosphaera watsonii	2021-09-10	-2	extreme	b		7.88	0.40	29.92	1.59	10082	0.0090	0.080		4.33	8.25	0.10	341	1130000
laboratory	Crocosphaera watsonii	2021-09-10	-2	extreme	c		7.82	0.39	29.90	0.17	9180	0.0090	0.110		4.20	7.46	0.13	326	1220000
laboratory	Crocosphaera watsonii	2021-09-11	-1	control	a	7.843	8.07	0.11	26.15	0.18	7246			0.02						6.22	51.65	8.31	10.03	-25.16												2650	7.96	26.00	38.85	8	15.21	560.06	561.83	2081.46	234.69	2331.36	3.63	5.46
laboratory	Crocosphaera watsonii	2021-09-11	-1	control	b	7.875	8.06	0.09	26.18	0.25	9918			0.02						6.02	61.35	10.19	4.28	-28.02												2650	7.96	26.00	38.85	8	15.21	560.06	561.83	2081.46	234.69	2331.36	3.63	5.46
laboratory	Crocosphaera watsonii	2021-09-11	-1	intermediate	a	7.989	7.90	0.01	27.52	0.13	9311			0.02						6.67	56.00	8.40	-5.89	-24.25												2650	7.87	28.00	38.93	8	19.08	738.24	740.52	2143.53	209.43	2372.04	3.26	4.89
laboratory	Crocosphaera watsonii	2021-09-11	-1	intermediate	b	7.971	7.97	0.01	28.11	0.11	5303			0.02						6.90	58.36	8.46	4.38	-24.03												2650	7.87	28.00	38.93	8	19.08	738.24	740.52	2143.53	209.43	2372.04	3.26	4.89
laboratory	Crocosphaera watsonii	2021-09-11	-1	intermediate	c	7.909	7.66	0.03	28.08	0.09	10115			0.02						6.41	53.26	8.31	3.57	-24.06												2650	7.87	28.00	38.93	8	19.08	738.24	740.52	2143.53	209.43	2372.04	3.26	4.89
laboratory	Crocosphaera watsonii	2021-09-11	-1	extreme	a	7.899	7.74	0.05	30.10	0.12	8213			0.02						5.90	46.59	7.89	-0.04	-22.23												2650	7.81	29.83	39.07	8	20.83	841.11	843.65	2168.31	199.41	2388.55	3.13	4.66
laboratory	Crocosphaera watsonii	2021-09-11	-1	extreme	b	7.749	7.79	0.06	30.11	0.08	9148			0.02						6.97	54.05	7.76	3.54	-24.42												2650	7.81	29.83	39.07	8	20.83	841.11	843.65	2168.31	199.41	2388.55	3.13	4.66
laboratory	Crocosphaera watsonii	2021-09-11	-1	extreme	c	7.847	7.65	0.02	29.70	0.39	7885			0.02						6.75	48.41	7.17	0.46	-24.31												2650	7.81	29.83	39.07	8	20.83	841.11	843.65	2168.31	199.41	2388.55	3.13	4.66
laboratory	Crocosphaera watsonii	2021-09-12	0	control	a	8.089	8.07	0.04	26.26	0.17	41500	0.0125	0.180	0.02	17.82	7.65	4.52	304	7210000	7.04	97.39	13.83	6.14	-29.15	1.46	35.15	1.70	40.90	40.26	0.90	0.850	11.42	20.12	1.63	1.52	2650	8.10	26.00	38.80	8	10.20	375.70	376.89	1925.67	298.51	2234.38	4.62	6.95
laboratory	Crocosphaera watsonii	2021-09-12	0	control	b	8.138	8.08	0.03	25.50	0.14	33000	0.0090	0.060	0.02	21.02	7.41	0.24	247	7500000	6.86	68.25	9.94	4.25	-26.37	1.69	40.47	1.65	39.63								2650	8.10	26.00	38.80	8	10.20	375.70	376.89	1925.67	298.51	2234.38	4.62	6.95
laboratory	Crocosphaera watsonii	2021-09-12	0	intermediate	a	7.882	7.89	0.03	27.10	0.71	29500	0.0090	0.200	0.02	21.71	7.53	-0.14	260	6920000	7.28	85.43	11.73	4.73	-25.27	3.69	88.55	1.40	33.68	48.70	13.27						2650	7.83	28.37	38.83	8	21.09	822.92	825.45	2180.05	194.53	2395.67	3.04	4.54
laboratory	Crocosphaera watsonii	2021-09-12	0	intermediate	b	7.877	7.95	0.04	28.15	0.11	26500	0.0090	0.120	0.02	29.53	8.29	0.09	374	7080000	7.48	79.25	10.60	4.30	-24.00	2.84	68.24	2.23	53.58			3.460	27.51	102.18	0.58	2.11	2650	7.83	28.37	38.83	8	21.09	822.92	825.45	2180.05	194.53	2395.67	3.04	4.54
laboratory	Crocosphaera watsonii	2021-09-12	0	intermediate	c	7.770	7.70	0.06	28.14	0.14	31000	0.0090	0.140	0.02	21.38	8.48	-0.06	236	8100000	8.80	95.35	10.83	2.85	-23.21	7.53	180.71	2.45	58.85								2650	7.83	28.37	38.83	8	21.09	822.92	825.45	2180.05	194.53	2395.67	3.04	4.54
laboratory	Crocosphaera watsonii	2021-09-12	0	extreme	a	7.724	7.73	0.05	30.14	0.11	30500	0.0235	1.080	0.02	19.51	7.71	1.79	322	8720000	6.56	74.23	11.32	-2.56	-26.41	2.48	59.45	2.13	51.24	50.55	1.57						2650	7.76	30.03	38.97	8	24.41	989.62	992.60	2216.22	179.76	2420.39	2.83	4.20
laboratory	Crocosphaera watsonii	2021-09-12	0	extreme	b	7.830	7.82	0.02	30.09	0.16	38000	0.0090	0.049	0.02	21.09	8.26	0.02	334	7300000	7.68	74.02	9.64	1.39	-25.11	2.40	57.51	2.15	51.65								2650	7.76	30.03	38.97	8	24.41	989.62	992.60	2216.22	179.76	2420.39	2.83	4.20
laboratory	Crocosphaera watsonii	2021-09-12	0	extreme	c	7.785	7.66	0.05	29.04	1.70	37000	0.0090	0.049	0.02	20.79	7.86	0.42	297	9450000	6.42	66.17	10.31	1.22	-26.05	3.48	83.57	2.03	48.75			1.502	3.06	5.67	1.07	1.94	2650	7.76	30.03	38.97	8	24.41	989.62	992.60	2216.22	179.76	2420.39	2.83	4.20
laboratory	Crocosphaera watsonii	2021-09-13	1	control	a	8.072	8.03	0.01	26.19	0.20	36000					7.23	0.46																			2650	8.07	25.90	38.90	8	11.27	414.46	415.77	1967.76	281.21	2260.24	4.34	6.54
laboratory	Crocosphaera watsonii	2021-09-13	1	control	b	8.086	8.04	0.01	25.42	0.09	39000					6.98	0.47																			2650	8.07	25.90	38.90	8	11.27	414.46	415.77	1967.76	281.21	2260.24	4.34	6.54
laboratory	Crocosphaera watsonii	2021-09-13	1	intermediate	a	7.942	7.91	0.00	27.65	0.06	22500					7.69	0.09																			2650	7.93	28.13	38.93	8	15.83	614.40	616.29	2082.32	234.60	2332.75	3.66	5.47
laboratory	Crocosphaera watsonii	2021-09-13	1	intermediate	b	7.973	7.97	0.01	28.08	0.10	27000					8.51	2.08																			2650	7.93	28.13	38.93	8	15.83	614.40	616.29	2082.32	234.60	2332.75	3.66	5.47
laboratory	Crocosphaera watsonii	2021-09-13	1	intermediate	c	7.898	7.75	0.00	28.23	0.08	24500					8.95	0.48																			2650	7.93	28.13	38.93	8	15.83	614.40	616.29	2082.32	234.60	2332.75	3.66	5.47
laboratory	Crocosphaera watsonii	2021-09-13	1	extreme	a	7.844	7.78	0.00	30.08	0.09	37000					7.78	0.34																			2650	7.85	29.93	39.07	8	18.58	751.84	754.11	2129.02	215.57	2363.17	3.39	5.04
laboratory	Crocosphaera watsonii	2021-09-13	1	extreme	b	7.876	7.83	0.01	30.02	0.19	34500					8.24	0.15																			2650	7.85	29.93	39.07	8	18.58	751.84	754.11	2129.02	215.57	2363.17	3.39	5.04
laboratory	Crocosphaera watsonii	2021-09-13	1	extreme	c	7.869	7.70	0.00	30.22	0.06	43500					7.76	0.18																			2650	7.85	29.93	39.07	8	18.58	751.84	754.11	2129.02	215.57	2363.17	3.39	5.04
laboratory	Crocosphaera watsonii	2021-09-14	2	control	a	8.034	8.02	0.03	26.38	0.25	37000	0.0225	0.060	0.02	21.02	8.15	0.25	510	8430000	6.99	83.91	12.00	7.10	-24.01	10.46	250.98	1.86	44.67	43.76	1.28	1.670	15.39	14.71	0.70	0.77	2650	8.03	25.85	39.05	8	12.53	460.42	461.88	2009.15	264.15	2285.82	4.07	6.14
laboratory	Crocosphaera watsonii	2021-09-14	2	control	b	8.049	8.04	0.02	25.52	0.10	38000	0.0090	0.049	0.02	20.73	7.49	0.16	325	7820000	7.49	86.71	11.57	7.26	-25.79	7.53	180.75	1.79	42.86								2650	8.03	25.85	39.05	8	12.53	460.42	461.88	2009.15	264.15	2285.82	4.07	6.14
laboratory	Crocosphaera watsonii	2021-09-14	2	intermediate	a	7.980	7.94	0.02	27.75	0.06	33000	0.0090	0.049	0.02	21.32	9.09	0.39	256	7580000	8.66	115.49	13.34	2.87	-23.73	7.80	187.17	2.22	53.36	55.79	4.20						2650	7.98	28.13	39.03	8	13.75	533.55	535.20	2029.95	255.99	2299.69	3.99	5.97
laboratory	Crocosphaera watsonii	2021-09-14	2	intermediate	b	7.999	8.00	0.02	28.06	0.19	23000	0.0325	0.049	0.02	28.30	7.71	0.29	404	8040000	8.44	108.66	12.87	3.58	-21.09	10.83	259.89	2.22	53.37			5.390	11.46	9.93	0.76	0.76	2650	7.98	28.13	39.03	8	13.75	533.55	535.20	2029.95	255.99	2299.69	3.99	5.97
laboratory	Crocosphaera watsonii	2021-09-14	2	intermediate	c	7.984	7.83	0.03	27.91	0.17	32000	0.0090	0.049	0.02	21.07	8.27	0.17	280	9020000	8.45	99.82	11.81	0.06	-24.34	19.02	456.56	2.53	60.64								2650	7.98	28.13	39.03	8	13.75	533.55	535.20	2029.95	255.99	2299.69	3.99	5.97
laboratory	Crocosphaera watsonii	2021-09-14	2	extreme	a	7.872	7.81	0.01	30.22	0.13	40000	0.0090	0.049	0.02	21.00	10.09	0.17	418	8910000	7.81	93.93	12.02	1.51	-24.35	10.15	243.57	1.95	46.88	49.17	2.25						2650	7.87	30.03	39.00	8	17.73	719.20	721.37	2112.63	222.36	2352.72	3.50	5.20
laboratory	Crocosphaera watsonii	2021-09-14	2	extreme	b	7.875	7.84	0.02	29.96	1.71	27000	0.0090	0.049	0.02	20.81	8.47	0.15	440	9730000	8.40	95.68	11.39	1.12	-23.83	5.36	128.53	2.05	49.26								2650	7.87	30.03	39.00	8	17.73	719.20	721.37	2112.63	222.36	2352.72	3.50	5.20
laboratory	Crocosphaera watsonii	2021-09-14	2	extreme	c	7.892	7.70	0.04	30.18	0.08	44000	0.0090	0.049	0.02	20.51	9.08	1.22	390	9650000	7.90	87.39	11.07	-4.88	-24.99	4.16	99.78	2.14	51.37			2.830	12.92	26.24	1.96	2.53	2650	7.87	30.03	39.00	8	17.73	719.20	721.37	2112.63	222.36	2352.72	3.50	5.20
laboratory	Crocosphaera watsonii	2021-09-15	3	control	a	8.105	8.03	0.04	26.37	0.13	32000	0.0095	0.770	0.02	21.29	7.29	0.17	360	8770000	1.63	22.54	13.81	51.38	-28.32												2650	8.08	26.05	38.85	8	10.76	396.89	398.15	1947.77	289.45	2247.98	4.48	6.74
laboratory	Crocosphaera watsonii	2021-09-15	3	control	b	8.082	8.03	0.01	25.71	0.17	33000	0.0095	0.220	0.02	20.94	6.74	0.18	321	7810000	2.27	26.31	11.60	57.83	-27.41												2650	8.08	26.05	38.85	8	10.76	396.89	398.15	1947.77	289.45	2247.98	4.48	6.74
laboratory	Crocosphaera watsonii	2021-09-15	3	intermediate	a	7.990	7.94	0.02	27.92	0.13	28000	0.0155	0.200	0.02	20.95	6.41	0.17	249	7340000	2.52	34.53	13.70	29.47	-25.69												2650	7.99	28.03	38.87	8	13.45	520.26	521.86	2023.35	258.81	2295.61	4.04	6.04
laboratory	Crocosphaera watsonii	2021-09-15	3	intermediate	b	8.000	8.01	0.02	28.05	0.17	34000	0.0090	0.350	0.02	21.09	8.36	0.07	372	7420000	2.06	30.29	14.71	37.59	-25.92												2650	7.99	28.03	38.87	8	13.45	520.26	521.86	2023.35	258.81	2295.61	4.04	6.04
laboratory	Crocosphaera watsonii	2021-09-15	3	intermediate	c	8.000	7.83	0.01	27.82	0.12	35000	0.0105	0.080	0.02	29.30	8.33	0.13	225	8770000	3.04	39.12	12.86	124.09	-26.12												2650	7.99	28.03	38.87	8	13.45	520.26	521.86	2023.35	258.81	2295.61	4.04	6.04
laboratory	Crocosphaera watsonii	2021-09-15	3	extreme	a	7.883	7.81	0.01	30.27	0.08	22000	0.0235	1.330	0.02	20.98	7.52	0.24	475	8160000	1.15	15.79	13.77	30.88	-27.17												2650	7.88	30.20	39.00	8	17.17	698.98	701.08	2100.01	227.56	2344.74	3.58	5.32
laboratory	Crocosphaera watsonii	2021-09-15	3	extreme	b	7.892	7.85	0.01	29.96	0.19	29000	0.0105	4.880	0.02	20.91	8.39	0.51	463	6710000	2.21	28.02	12.68	9.64	-26.57												2650	7.88	30.20	39.00	8	17.17	698.98	701.08	2100.01	227.56	2344.74	3.58	5.32
laboratory	Crocosphaera watsonii	2021-09-15	3	extreme	c	7.892	7.71	0.01	30.18	0.10	30500	0.0115	1.080	0.02	20.28	7.35	0.19	406	9890000	2.14	32.72	15.29	105.63	-27.36												2650	7.88	30.20	39.00	8	17.17	698.98	701.08	2100.01	227.56	2344.74	3.58	5.32
laboratory	Crocosphaera watsonii	2021-09-16	4	control	a	8.141	8.04	0.05	26.37	0.15	36000	0.0090	0.060		21.24	6.90	0.11	403	8820000	7.59	90.08	11.87	31.63	-25.86	6.96	167.03	2.13	51.00	48.26	3.87	0.450	1.98	3.53	0.13	0.08	2650	8.04	26.10	38.90	8	12.09	446.62	448.03	1992.76	271.01	2275.86	4.19	6.31
laboratory	Crocosphaera watsonii	2021-09-16	4	control	b	8.077	8.01	0.01	25.71	0.11	32000	0.0090	0.420		20.79	6.87	0.60	381	8030000	7.51	93.05	12.38	26.24	-26.25	1.60	38.42	1.90	45.53								2650	8.04	26.10	38.90	8	12.09	446.62	448.03	1992.76	271.01	2275.86	4.19	6.31
laboratory	Crocosphaera watsonii	2021-09-16	4	intermediate	a	7.980	7.93	0.01	27.90	0.09	36000	0.0090	1.410		21.11	8.21	0.24	265	6710000	7.57	95.93	12.67	6.75	-24.21	5.32	127.63	2.27	54.53	54.56	2.25						2650	7.96	28.07	38.93	8	14.63	566.57	568.32	2054.91	245.83	2315.37	3.83	5.73
laboratory	Crocosphaera watsonii	2021-09-16	4	intermediate	b	7.979	7.99	0.01	28.19	0.21	32000	0.0090	0.050		29.45	8.65	-0.09	403	7180000	8.34	105.95	12.71	8.36	-23.66	9.24	221.73	2.18	52.33			7.150	0.04	0.08	0.12	0.10	2650	7.96	28.07	38.93	8	14.63	566.57	568.32	2054.91	245.83	2315.37	3.83	5.73
laboratory	Crocosphaera watsonii	2021-09-16	4	intermediate	c	7.982	7.82	0.00	27.82	0.10	41500	0.0090	0.340		20.90	8.70	0.01	242	8560000	10.07	110.47	10.97	97.26	-24.00	8.89	213.43	2.37	56.83								2650	7.96	28.07	38.93	8	14.63	566.57	568.32	2054.91	245.83	2315.37	3.83	5.73
laboratory	Crocosphaera watsonii	2021-09-16	4	extreme	a	7.853	7.79	0.00	30.28	0.09	23300	0.0145	0.810		20.88	9.11	0.18	438	7250000	7.99	94.05	11.77	12.80	-24.14	1.19	28.55	1.16	27.79	36.86	12.70						2650	7.86	29.73	39.10	8	18.26	736.11	738.34	2122.57	218.17	2359.00	3.42	5.09
laboratory	Crocosphaera watsonii	2021-09-16	4	extreme	b	7.876	7.84	0.01	29.94	0.11	22500	0.0090	0.270		21.00	9.05	0.23	449	5000000	7.57	79.65	10.52	3.68	-21.67	1.17	27.99	1.31	31.42								2650	7.86	29.73	39.10	8	18.26	736.11	738.34	2122.57	218.17	2359.00	3.42	5.09
laboratory	Crocosphaera watsonii	2021-09-16	4	extreme	c	7.881	7.71	0.00	30.18	0.08	23500	0.0090	0.490		20.31	8.48	0.12	387	9550000	7.31	86.51	11.84	11.65	-24.74	1.33	32.03	2.14	51.37			0.810	1.13	1.15	0.10	0.13	2650	7.86	29.73	39.10	8	18.26	736.11	738.34	2122.57	218.17	2359.00	3.42	5.09
laboratory	Crocosphaera watsonii	2021-09-17	5	control	a		8.01	0.01	26.45	0.16		0.0090	0.220		21.20	0.42	0.00			7.53	99.70	13.24	56.28	-25.73												2650	8.04	26.25	38.95	8	12.07	447.47	448.89	1990.71	271.83	2274.61	4.20	6.33
laboratory	Crocosphaera watsonii	2021-09-17	5	control	b		7.98	0.01	25.76	0.12		0.0115	0.760		20.25	0.29	0.00			7.43	92.86	12.50	35.64	-25.18												2650	8.04	26.25	38.95	8	12.07	447.47	448.89	1990.71	271.83	2274.61	4.20	6.33
laboratory	Crocosphaera watsonii	2021-09-17	5	intermediate	a		7.91	0.01	27.90	0.06		0.0090	0.210		21.01	0.40	0.00			7.35	102.58	13.96	12.55	-23.65												2650	7.96	28.07	38.93	8	14.60	565.59	567.33	2054.26	246.10	2314.96	3.84	5.74
laboratory	Crocosphaera watsonii	2021-09-17	5	intermediate	b		7.97	0.00	28.03	0.15		0.0115	0.610		29.40	0.43	0.00			7.87	106.51	13.54	29.85	-23.94												2650	7.96	28.07	38.93	8	14.60	565.59	567.33	2054.26	246.10	2314.96	3.84	5.74
laboratory	Crocosphaera watsonii	2021-09-17	5	intermediate	c		7.81	0.00	27.81	0.08		0.0095	0.610		20.90	0.49	0.00			9.51	111.07	11.67	112.58	-24.36												2650	7.96	28.07	38.93	8	14.60	565.59	567.33	2054.26	246.10	2314.96	3.84	5.74
laboratory	Crocosphaera watsonii	2021-09-17	5	extreme	a		7.77	0.00	30.28	0.10		0.0090	0.290		21.18	0.25	0.00			6.56	81.03	12.35	20.40	-23.50												2650	7.86	29.87	39.03	8	18.29	739.53	741.76	2122.82	218.13	2359.24	3.43	5.10
laboratory	Crocosphaera watsonii	2021-09-17	5	extreme	b		7.81	0.00	29.97	0.09		0.0115	0.300		21.38	0.50	0.00			6.03	61.31	10.17	16.89	-24.34												2650	7.86	29.87	39.03	8	18.29	739.53	741.76	2122.82	218.13	2359.24	3.43	5.10
laboratory	Crocosphaera watsonii	2021-09-17	5	extreme	c		7.73	0.01	30.16	0.06		0.0090	0.170		20.59	0.39	0.00			6.52	96.23	14.75	10.95	-23.84												2650	7.86	29.87	39.03	8	18.29	739.53	741.76	2122.82	218.13	2359.24	3.43	5.10