Kapshyna Iryna iryna.kapshyna@senckenberg.de 0000-0003-4758-5715 Veit-Köhler Gritta gveit-koehler@senckenberg.de https://www.senckenberg.de/de/institute/sam/dzmb/fb-oekologische-biodiversitaetsforschung/ 0000-0002-3453-898X Wilsenack Jule Steiling Josefine Khodami Sahar 2024 Monitoring of sandy beach meiofauna before and after sand nourishment (Ahrenshoop, Baltic Sea): metabarcoding data (number of reads per operational taxonomic unit) dataset https://doi.org/10.1594/PANGAEA.971242 2024-12-23T07:14:59 We provide metabarcoding data (number of reads per operational taxonomic unit, OTU) determined from sediment samples collected on the sandy-beach water line of Ahrenshoop (Baltic Sea). Five sampling stations lay within the zone impacted by the sand nourishment between the boundary of the nature reserve in the north east and a site just north of the breakwater (AH01-AH05). An unaffected reference station was located south of Ahrenshoop (close to Niehagen) at the end of the road Pappelallee (PAP). Samples were collected at four dates. The first sampling was carried out before the sand nourishment took place (T0: 14 and 16 September 2021). Three samplings were realised after the impact: T1 (23 March 2022), T2 (27 September 2022), and T3 (28 March 2023). Latitude and longitude of each sampling location per station were recorded at each sampling date using a hand-held GPS application on a mobile phone. At the stations sampling locations varied over time. Prior to the sand nourishment the beach was narrow due to sand erosion in previous years. After the nourishment the additional extent of the beach was approximately 40 m at sampling date T1. Subsequently, progressive sand erosion forced the sampling locations (situated at the water line) further inland at T2 and T3. Samples were taken from the beach-water interface (water line) in the middle of the area between two groynes. Plexiglass cores (inner core diameter 5.4 cm) were inserted vertically into the sediment down to 15 cm depth. Each core was sliced in 5 cm-layers (0-5, 5-10 and 10-15 cm). Sediment horizons were preserved in 96-99% ethanol. Three cores (2 cores at T0) per sampling date were taken for metabarcoding analyses. The organisms were extracted by decantation over a 32-μm sieve. Genomic DNA was extracted from the filters using the DNeasy PowerSoil pro kit (Qiagen). Realtime-PCR was performed to amplify V1&V2, two hypervariable regions of 18S rDNA gene. The sequencing run was performed using the MiSeq Reagent Nanokit v2 (250 cycles paired end) on an Illumina MiSeq platform at the DZMB Metabarcoding lab in Wilhelmshaven, Germany. High-resolution amplicon sequence variants (ASVs) were obtained and compared to the NCBI database to assign taxonomic information to each ASV. The target meiofauna ASVs were further classified into operational taxonomic units (OTUs) with a 3% cut-off threshold using the statistical software R. Here, we present two Tables: (1) the taxonomic description of the 843 OTUs and their assigned ID number; (2) the number of reads per OTU per sample. The metabarcoding data are part of a larger ecological study on the influence of sand nourishment on meiofauna communities, which included grain-size and meiofauna abundances (see Related to and Supplement to). Kapshyna Iryna iryna.kapshyna@senckenberg.de 0000-0003-4758-5715 Veit-Köhler Gritta gveit-koehler@senckenberg.de https://www.senckenberg.de/de/institute/sam/dzmb/fb-oekologische-biodiversitaetsforschung/ 0000-0002-3453-898X Hoffman Leon leon.hoffman@senckenberg.de Khodami Sahar 2024 Impact of a coastal protection measure on sandy-beach meiofauna at Ahrenshoop (Baltic Sea, Germany): results from metabarcoding and morphological approaches are similar journal article Metabarcoding and Metagenomics 8 https://doi.org/10.3897/mbmg.8.127688 e127688 Kapshyna Iryna iryna.kapshyna@senckenberg.de 0000-0003-4758-5715 Fischer Tobias 0000-0002-3467-0649 Veit-Köhler Gritta gveit-koehler@senckenberg.de https://www.senckenberg.de/de/institute/sam/dzmb/fb-oekologische-biodiversitaetsforschung/ 0000-0002-3453-898X 2024 Monitoring of sandy beach meiofauna before and after sand nourishment (Ahrenshoop, Baltic Sea): abundance data of meiofauna higher taxa dataset https://doi.org/10.1594/PANGAEA.971240 12.391431 12.443088 54.356681 54.397566 12.421558208333332 54.382465291666676 2021-09-14T00:00:00 2023-03-28T00:00:00 Deutsche Bundesstiftung Umwelt DBU https://www.dbu.de/ https://doi.org/10.13039/100007636 30023/025 Influence of coastal protection measures on the biodiversity of copepods (Ahrenshoop, Baltic Sea): Comparison of quantitative and qualitative methods Niedersächsisches Ministerium für Wissenschaft und Kultur https://doi.org/10.13039/501100010570 15-76251-3-23/5396/2022 Biodiversity and coastal protection: Influence of sand nourishment on communities of interstitial animals T0_AH01 T0 54.397422 12.443088 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2021-09-14T00:00:00 Push corer PUC T0_AH02 54.391532 12.436409 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2021-09-16T00:00:00 Push corer PUC T0_AH03 T0 54.387604 12.429798 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2021-09-14T00:00:00 Push corer PUC T0_AH04 54.382261 12.418044 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2021-09-14T00:00:00 Push corer PUC T0_AH05 T0 54.378733 12.411166 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2021-09-14T00:00:00 Push corer PUC T0_PAP T0 54.356765 12.391492 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2021-09-14T00:00:00 Push corer PUC T1_AH01 T1 54.397566 12.442567 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-03-24T00:00:00 Push corer PUC T1_AH02 54.391779 12.436003 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-03-24T00:00:00 Push corer PUC T1_AH03 T1 54.387805 12.42956 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-03-24T00:00:00 Push corer PUC T1_AH04 54.382376 12.417723 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-03-24T00:00:00 Push corer PUC T1_AH05 T1 54.378831 12.410925 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-03-24T00:00:00 Push corer PUC T1_PAP T1 54.356766 12.391431 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-03-24T00:00:00 Push corer PUC T2_AH01 T2 54.397554 12.442897 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-09-27T00:00:00 Push corer PUC T2_AH02 54.391655 12.436157 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-09-27T00:00:00 Push corer PUC T2_AH03 T2 54.387737 12.429541 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-09-27T00:00:00 Push corer PUC T2_AH04 54.382477 12.417818 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-09-27T00:00:00 Push corer PUC T2_AH05 T2 54.378818 12.411049 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-09-27T00:00:00 Push corer PUC T2_PAP T2 54.356778 12.391517 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2022-09-27T00:00:00 Push corer PUC T3_AH01 T3 54.397402 12.442908 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2023-03-28T00:00:00 Push corer PUC T3_AH02 54.39155 12.436344 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2023-03-28T00:00:00 Push corer PUC T3_AH03 T3 54.387753 12.429738 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2023-03-28T00:00:00 Push corer PUC T3_AH04 54.38238 12.418042 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2023-03-28T00:00:00 Push corer PUC T3_AH05 T3 54.378942 12.411546 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2023-03-28T00:00:00 Push corer PUC T3_PAP T3 54.356681 12.391634 Ahrenshoop, Mecklenburg-Vorpommern, Germany 2023-03-28T00:00:00 Push corer PUC 4 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Related to the project: * ECAS Baltic: Strategies of ecosystem-friendly coastal protection and ecosystem-supporting coastal adaptation for the German Baltic Sea Coast, Web: https://deutsche-kuestenforschung.de/ecas-baltic.html Binary Object Binary Kapshyna Iryna iryna.kapshyna@senckenberg.de 0000-0003-4758-5715 Binary File content Content Kapshyna Iryna iryna.kapshyna@senckenberg.de 0000-0003-4758-5715 Content CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable Ahrenshoop Baltic Sea community composition Fischland-Darß-Zingst Peninsula meiofauna Metabarcoding number of reads potential species sand nourishment sandy beach sediment depth layers 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