Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Tillmann Urban Urban.Tillmann@awi.de 0000-0002-8207-4382 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Pöchhacker Magdalena Department of Food Chemistry and Toxicology, University of Vienna https://lmc.univie.ac.at/ https://ror.org/03prydq77 Varga Elisabeth Department of Food Chemistry and Toxicology, University of Vienna https://lmc.univie.ac.at/ https://ror.org/03prydq77 Krock Bernd Bernd.Krock@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/bernd-krock.html Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Koch Florian florian.koch@awi.de 0000-0001-7107-4160 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Harris Thomas M Department of Chemistry, Vanderbilt University https://as.vanderbilt.edu/chemistry/ https://ror.org/02vm5rt34 Meunier Cédric Léo Cedric.Meunier@awi.de 0000-0002-4070-4286 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 2024 Bioassays quantifying the adverse effects of purified goniodomins (GDs) on the membrane integrity of the RTgill-W1 cell line of the rainbow trout (Oncorhynchus mykiss) dataset https://doi.org/10.1594/PANGAEA.968673 2024-08-27T10:40:43 https://doi.org/10.1594/PANGAEA.968675 RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) were subjected to purified goniodomins (GDs) for 24 h to create dose-response curves. The bioassays were carried out with 100 µl of total incubation volume in 96-well plates. After 24 hours, 50 µl subsamples were transferred to a new 96-well plate for the assessment of lytic activity using a lactate dehydrogenase (LDH) cytotoxicity assay kit (CyQUANT™, Thermo Fisher Scientific, C20301) following the standard protocol provided by the manufacturer. The bioassays were analysed fluorometrically using a cell-imaging multi-mode reader (Cytation™ 3 Cell Imaging Multi-Mode Reader, BioTek). All data was pre-filtered according to (1) visual inspection of the gill cell densities in the 96-well plates and if densities differed greatly data was excluded, and (2) data were grouped by each biological gill cell line replicate, each biological A. pseudogonyaulax replicate, each strain and each cell density equivalent and subjected to a Dixon outlier test. If the Dixon test was significant, data was excluded. This dataset contains three biological gill cell line replicates, each tested with three to four concentrations of GDA, GDA-sa or GDA + GDA-sa each comprised of two to three technical replicates. The bioassays were carried out between August 23 and October 7, 2023 at the Department of Food Chemistry and Toxicology (LMC), Faculty of Chemistry, University of Vienna. Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Tillmann Urban Urban.Tillmann@awi.de 0000-0002-8207-4382 Pöchhacker Magdalena Varga Elisabeth Krock Bernd Bernd.Krock@awi.de https://www.awi.de/en/about-us/organisation/staff/single-view/bernd-krock.html Porreca Francesco f.porreca98@gmail.com Koch Florian florian.koch@awi.de 0000-0001-7107-4160 Harris Thomas M Meunier Cédric Léo Cedric.Meunier@awi.de 0000-0002-4070-4286 2024 Toxic effects of the emerging Alexandrium pseudogonyaulax (Dinophyceae) on multiple trophic levels of the pelagic food web journal article Harmful Algae https://doi.org/10.1016/j.hal.2024.102705 102705 not specified German Federal Environmental Foundation DBU https://www.dbu.de/ https://doi.org/10.13039/100007636 PhD Scholarship Helmholtz Association of German Research Centres HGF https://www.helmholtz.de https://doi.org/10.13039/501100001656 Changing-Earth_Subtopic_6.2 Adaptation of marine life: From genes to ecosystems https://earthenvironment.helmholtz.de/changing-earth/topics/topic-6-marine-and-polar-life/#c115556 946 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Type of study Study type type Bioassay Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Study type Date/time start, experiment Date/time start exp Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Date/time start exp Date/time end, experiment Date/time end exp Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Date/time end exp Incubation duration Inc dur h Time T http://dbpedia.org/resource/Time Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Inc dur [h] Incubation volume Inc vol µl Volume http://dbpedia.org/resource/Volume Microwell plate incubation Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 96-well plate Inc vol [µl] Treatment: toxin T:toxin treatment toxin http://purl.obolibrary.org/obo/CHEBI_27026 Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Goniodomin (GD) congener: GDA = goniodomin A, GDA-sa = goniodomin A seco acid, GDA/GDA-sa = mixture of goniodomin A and its seco acid T:toxin Goniodomin GD µg/l goniodomin Density http://dbpedia.org/resource/Density Diluted with K-medium to obtain different concentrations Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 GD [µg/l] Replicate, technical Repl tech Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Repl tech Species, unique identification Species UID species RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) Species UID Species, unique identification (URI) Species UID (URI) species RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) Species UID (URI) Species, unique identification (Semantic URI) Species UID (Semantic URI) species RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) Species UID (Semantic URI) Replicate, biological Repl bio Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Biological RTgill-W1 gill cell replicate; gill cell densities in the 96-well plates were visually inspected and data were excluded if densities differed greatly Repl bio Extracellular lactate dehydrogenase activity, gill cells of Oncorhynchus mykiss, relative eLDH act gill cells O. mykiss rel % Oncorhynchus mykiss https://www.marinespecies.org/aphia.php?p=taxdetails&id=127185 relative Dimensionless Ratio ψ CyQUANTTM™ lactate dehydrogenase (LDH) cytotoxicity assay kit, Thermo Fisher Scientific, C20301; measured with cell imaging multi-mode reader, BioTek, Cytation™ 3 Möller Kristof kristof-moeller@outlook.de 0000-0002-4590-6223 Lytic activity (as percentage of control) was assessed using the CyQUANT™ lactate dehydrogenase (LDH) cytotoxicity assay kit (Thermo Fisher Scientific, C20301) following the standard protocol provided by the manufacturer; assay results were assessed fluorometrically using a cell-imaging multi-mode reader (Cytation™ 3 Cell Imaging Multi-Mode Reader, BioTek). eLDH act gill cells O. mykiss rel [%] CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable Alexandrium sp. Bioactive extracellular substances (BECs) Cell titre blue (CTB) Laboratory experiment lactate dehydrogenase (LDH) Oncorhynchus mykiss phycotoxins viability GFBio NFDI4BioDiversity author105367 author105368 author105369 author105430 author37685 author39556 author54494 author58376 author98695 award1661 award2381 curlevel30 funder20477 funder20792 inst21564 inst21565 inst32 journal6310 license21 method16748 method16790 method16802 method9043 param185520 param25453 param510679 param516119 param516120 param528086 param528662 param528801 param528802 param528803 param528804 pi98695 ref125959 term1074789 term1168883 term233 term38841 term41013 term42421 term42484 term43006 term43792 term43794 term43975 term43984 term44355 term49384 term49506 term54505 term67417 term68112 term70553 term74165 term74166 topotype1