de.pangaea.dataset968673 und de.pangaea.dataset968675 dataset PANGAEA
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https://www.pangaea.de/ information pointOfContact 2026-02-15T12:59:32Z https://doi.pangaea.de/10.1594/PANGAEA.968673 <gco:CharacterString>Bioassays quantifying the adverse effects of purified goniodomins (GDs) on the membrane integrity of the RTgill-W1 cell line of the rainbow trout (Oncorhynchus mykiss)</gco:CharacterString> 2024-08-27T10:40:43 publication https://doi.org/10.1594/PANGAEA.968673 Möller, Kristof
kristof-moeller@outlook.de
https://orcid.org/0000-0002-4590-6223 information author Tillmann, Urban
Urban.Tillmann@awi.de
https://orcid.org/0000-0002-8207-4382 information author Pöchhacker, Magdalena author Varga, Elisabeth author Krock, Bernd
Bernd.Krock@awi.de
https://www.awi.de/en/about-us/organisation/staff/single-view/bernd-krock.html information author Koch, Florian
florian.koch@awi.de
https://orcid.org/0000-0001-7107-4160 information author Harris, Thomas M author Meunier, Cédric Léo
Cedric.Meunier@awi.de
https://orcid.org/0000-0002-4070-4286 information author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) were subjected to purified goniodomins (GDs) for 24 h to create dose-response curves. The bioassays were carried out with 100 µl of total incubation volume in 96-well plates. After 24 hours, 50 µl subsamples were transferred to a new 96-well plate for the assessment of lytic activity using a lactate dehydrogenase (LDH) cytotoxicity assay kit (CyQUANT™, Thermo Fisher Scientific, C20301) following the standard protocol provided by the manufacturer. The bioassays were analysed fluorometrically using a cell-imaging multi-mode reader (Cytation™ 3 Cell Imaging Multi-Mode Reader, BioTek). All data was pre-filtered according to (1) visual inspection of the gill cell densities in the 96-well plates and if densities differed greatly data was excluded, and (2) data were grouped by each biological gill cell line replicate, each biological A. pseudogonyaulax replicate, each strain and each cell density equivalent and subjected to a Dixon outlier test. If the Dixon test was significant, data was excluded. This dataset contains three biological gill cell line replicates, each tested with three to four concentrations of GDA, GDA-sa or GDA + GDA-sa each comprised of two to three technical replicates. The bioassays were carried out between August 23 and October 7, 2023 at the Department of Food Chemistry and Toxicology (LMC), Faculty of Chemistry, University of Vienna. completed Möller, Kristof
kristof-moeller@outlook.de
https://orcid.org/0000-0002-4590-6223 information principalInvestigator Alexandrium sp. Bioactive extracellular substances (BECs) Cell titre blue (CTB) Laboratory experiment lactate dehydrogenase (LDH) Oncorhynchus mykiss phycotoxins viability theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation Type of study thematicClassification Bioassay Date/time start, experiment thematicClassification Date/time end, experiment thematicClassification Incubation duration physicalMeasurement h h Incubation volume physicalMeasurement Microwell plate incubation; 96-well plate µl µl Treatment: toxin thematicClassification Goniodomin (GD) congener: GDA = goniodomin A, GDA-sa = goniodomin A seco acid, GDA/GDA-sa = mixture of goniodomin A and its seco acid Goniodomin physicalMeasurement Diluted with K-medium to obtain different concentrations µg/l µg/l Replicate, technical thematicClassification Species, unique identification thematicClassification RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) Species, unique identification (URI) thematicClassification RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) Species, unique identification (Semantic URI) thematicClassification RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) Replicate, biological thematicClassification Biological RTgill-W1 gill cell replicate; gill cell densities in the 96-well plates were visually inspected and data were excluded if densities differed greatly Extracellular lactate dehydrogenase activity, gill cells of Oncorhynchus mykiss, relative physicalMeasurement CyQUANTTM™ lactate dehydrogenase (LDH) cytotoxicity assay kit, Thermo Fisher Scientific, C20301; measured with cell imaging multi-mode reader, BioTek, Cytation™ 3; Lytic activity (as percentage of control) was assessed using the CyQUANT™ lactate dehydrogenase (LDH) cytotoxicity assay kit (Thermo Fisher Scientific, C20301) following the standard protocol provided by the manufacturer; assay results were assessed fluorometrically using a cell-imaging multi-mode reader (Cytation™ 3 Cell Imaging Multi-Mode Reader, BioTek). % % text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.968673?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. Supplement to <gco:CharacterString>Toxic effects of the emerging Alexandrium pseudogonyaulax (Dinophyceae) on multiple trophic levels of the pelagic food web</gco:CharacterString> 2024-01-01 publication https://doi.org/10.1016/j.hal.2024.102705 Möller, Kristof
kristof-moeller@outlook.de
https://orcid.org/0000-0002-4590-6223 information author Tillmann, Urban
Urban.Tillmann@awi.de
https://orcid.org/0000-0002-8207-4382 information author Pöchhacker, Magdalena author Varga, Elisabeth author Krock, Bernd
Bernd.Krock@awi.de
https://www.awi.de/en/about-us/organisation/staff/single-view/bernd-krock.html information author Porreca, Francesco
f.porreca98@gmail.com
author Koch, Florian
florian.koch@awi.de
https://orcid.org/0000-0001-7107-4160 information author Harris, Thomas M author Meunier, Cédric Léo
Cedric.Meunier@awi.de
https://orcid.org/0000-0002-4070-4286 information author documentDigital Harmful Algae 102705