PANGAEA_968673 Bioassays quantifying the adverse effects of purified goniodomins (GDs) on the membrane integrity of the RTgill-W1 cell line of the rainbow trout (Oncorhynchus mykiss) Möller, Kristof; Tillmann, Urban; Pöchhacker, Magdalena; Varga, Elisabeth; Krock, Bernd; Koch, Florian; Harris, Thomas M; Meunier, Cédric Léo Bioassays quantifying the adverse effects of purified goniodomins (GDs) on the membrane integrity of the RTgill-W1 cell line of the rainbow trout (Oncorhynchus mykiss) 2024-08-27 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.968673 Investigator Kristof Möller kristof-moeller@outlook.de Earth Science Type of study Date/time start, experiment Date/time end, experiment Incubation duration Incubation volume Treatment: toxin Goniodomin Replicate, technical Species, unique identification Species, unique identification (URI) Species, unique identification (Semantic URI) Replicate, biological Extracellular lactate dehydrogenase activity, gill cells of Oncorhynchus mykiss, relative geoscientificInformation Alexandrium sp. Bioactive extracellular substances (BECs) Cell titre blue (CTB) Laboratory experiment lactate dehydrogenase (LDH) Oncorhynchus mykiss phycotoxins viability Bioassay Microwell plate incubation Diluted with K-medium to obtain different concentrations CyQUANTTM™ lactate dehydrogenase (LDH) cytotoxicity assay kit, Thermo Fisher Scientific, C20301; measured with cell imaging multi-mode reader, BioTek, Cytation™ 3 Complete unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 946 data points text/tab-separated-values Möller, Kristof; Tillmann, Urban; Pöchhacker, Magdalena; Varga, Elisabeth; Krock, Bernd; Porreca, Francesco; Koch, Florian; Harris, Thomas M; Meunier, Cédric Léo (2024): Toxic effects of the emerging Alexandrium pseudogonyaulax (Dinophyceae) on multiple trophic levels of the pelagic food web. Harmful Algae, 102705, https://doi.org/10.1016/j.hal.2024.102705

RTgill-W1 gill cells of the rainbow trout (Oncorhynchus mykiss) were subjected to purified goniodomins (GDs) for 24 h to create dose-response curves. The bioassays were carried out with 100 µl of total incubation volume in 96-well plates. After 24 hours, 50 µl subsamples were transferred to a new 96-well plate for the assessment of lytic activity using a lactate dehydrogenase (LDH) cytotoxicity assay kit (CyQUANT™, Thermo Fisher Scientific, C20301) following the standard protocol provided by the manufacturer. The bioassays were analysed fluorometrically using a cell-imaging multi-mode reader (Cytation™ 3 Cell Imaging Multi-Mode Reader, BioTek). All data was pre-filtered according to (1) visual inspection of the gill cell densities in the 96-well plates and if densities differed greatly data was excluded, and (2) data were grouped by each biological gill cell line replicate, each biological A. pseudogonyaulax replicate, each strain and each cell density equivalent and subjected to a Dixon outlier test. If the Dixon test was significant, data was excluded. This dataset contains three biological gill cell line replicates, each tested with three to four concentrations of GDA, GDA-sa or GDA + GDA-sa each comprised of two to three technical replicates. The bioassays were carried out between August 23 and October 7, 2023 at the Department of Food Chemistry and Toxicology (LMC), Faculty of Chemistry, University of Vienna. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.968673"!

https://doi.org/10.1016/j.hal.2024.102705 Toxic effects of the emerging Alexandrium pseudogonyaulax (Dinophyceae) on multiple trophic levels of the pelagic food web PANGAEA_968675 DIF 9.4 2024-08-27 2026-02-15