Rehder, Linda; Rokitta, Sebastian D; Buschmann, Isabelle; Jasper, Levke; Hoppe, Clara Jule Marie; Rost, Björn: Temperature responses of different functional traits in Arctic phytoplankton [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.968670 (dataset in review)
Abstract:
This study was conducted in autumn/winter 2021/2022 at the laboratory of the Alfred-Wegener-Institute Bremerhaven. To assess temperature response patterns of various functional traits in Arctic phytoplankton species, we cultivated Thalassiosira hyalina, Phaeocystis pouchetii, Micromonas pusilla and Nitzschia frigida at a temperature gradient of 0°C to 14°C under saturating light of 100 µmol photons m-2 s-1 (T. hyalina, P. pouchetii, M. pusilla) or 30 µmol photons m-2 s-1 (N. frigida) and replete nutrient conditions (micronutrients following F/2; 100 µmol L-1 nitrate, 100 µmol L-1 silicate, 6 µmol L-1 phosphate) in dilute batch cultures. Culture bottles were aerated with air containing a pCO2 of 400 µatm ensure a stabilized carbonate chemistry and to avoid sedimentation. After an acclimation period of >7 generation or >10 days, respectively, various functional traits were obtained: specific growth rate, quotas of particulate organic carbon (POC) and nitrogen (PON) as well as chlorophyll a (Chl a), photo-physiology using fast repetition rate fluorometry. Furthermore, the following parameters were calculated from the obtained data: POC:PON, Chl a:POC, POC production rate, photosystem II quantum yield, absolute electron transport rates at in-situ irradiance and maximal irradiance, light use efficiency, light acclimation indices, lifetime of PSII reopening and absorption cross section.
Comment:
Thalassiosira hylina strain KB3SS5, isolated 2020 in Kongsjford (Svalbard)
Phaeocystis pouchetii strain PS78, isolated 2012 in the Fram strait
Micromonas pusilla strain CLP1, isolated 2014 in Kongsfjord (Svalbard)
Nitzschia frigida strain UIO-542, isolated in 2019 in the Fram strait
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Rehder, Linda | |||
| 2 | Sampling date | Sampling date | Rehder, Linda | |||
| 3 | Sample ID | Sample ID | Rehder, Linda | |||
| 4 | Type of study | Study type | Rehder, Linda | |||
| 5 | Laboratory | Lab | Rehder, Linda | |||
| 6 | Species, unique identification | Species UID | Rehder, Linda | |||
| 7 | Species, unique identification (URI) | Species UID (URI) | Rehder, Linda | |||
| 8 | Strain | Strain | Rehder, Linda | |||
| 9 | Time in years | Time | a | Rehder, Linda | isolation year | |
| 10 | Location | Location | Rehder, Linda | isolation coordinates | ||
| 11 | Treatment: temperature | T:temp | °C | Rehder, Linda | ||
| 12 | Treatment: partial pressure of carbon dioxide | T:pCO2 | µatm | Rehder, Linda | ||
| 13 | Treatment: light:dark cycle | T:L:D | hh:hh | Rehder, Linda | ||
| 14 | Treatment: light intensity | T:Io | µmol/m2/s | Rehder, Linda | ||
| 15 | Treatment: salinity | T:sal | Rehder, Linda | |||
| 16 | Medium | Medium | Rehder, Linda | |||
| 17 | Generation | Generation | Rehder, Linda | # in acclimation | ||
| 18 | Growth rate | µ | 1/day | Rehder, Linda | Daily cell counting, Beckmann coulter Counter | |
| 19 | Carbon, organic, particulate, per cell | POC/cell | pg/# | Rehder, Linda | Flash combustions technique, CN Analyzer, EuroVector EA 3000 | |
| 20 | Nitrogen, organic, particulate, per cell | PON/cell | pg/# | Rehder, Linda | Flash combustions technique, CN Analyzer, EuroVector EA 3000 | |
| 21 | Carbon, organic, particulate/Nitrogen, organic, particulate ratio | POC/PON | mol/mol | Rehder, Linda | Calculated | |
| 22 | Chlorophyll a per cell | Chl a/cell | pg/# | Rehder, Linda | Fluorometry | including acidification step (Knap et al. 1996; https://doi.org/10.25607/obp-1409) |
| 23 | Chlorophyll a/particulate organic carbon ratio | Chl a/POC | Rehder, Linda | Calculated | in pg/pg | |
| 24 | Carbon, organic, particulate, net production, per cell | NP POC/cell | pg/#/day | Rehder, Linda | Calculated | =µ*POC/cell |
| 25 | Absorption cross section of PSII photochemistry | nm**2/PSII | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | ||
| 26 | Time constant for the initial part of an single turnover relaxation phase | Tau | µs | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | |
| 27 | Quantum yield efficiency of photosystem II | Fv/Fm | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | ||
| 28 | Electron transport rate, absolute | aETR | e/PSII/s | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | abs ETR in-situ (e- PSII-1 s-1) |
| 29 | Light use efficiency | alpha | µmol/m2/s | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | |
| 30 | Light saturation | Ek | µmol/m2/s | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | |
| 31 | Maximal electron transport rate | ETR max | e/PSII/s | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | abs ETR Vmax (e- PSII-1 s-1) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2286 data points
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