Rehder, Linda; Rokitta, Sebastian D; Buschmann, Isabelle; Jasper, Levke; Hoppe, Clara Jule Marie; Rost, Björn (2024): Temperature responses of different functional traits in Arctic phytoplankton [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.968670
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Abstract:
This study was conducted in autumn/winter 2021/2022 at the laboratory of the Alfred-Wegener-Institute Bremerhaven. To assess temperature response patterns of various functional traits in Arctic phytoplankton species, we cultivated Thalassiosira hyalina, Phaeocystis pouchetii, Micromonas pusilla and Nitzschia frigida at a temperature gradient of 0°C to 14°C under saturating light of 100 µmol photons m-2 s-1 (T. hyalina, P. pouchetii, M. pusilla) or 30 µmol photons m-2 s-1 (N. frigida) and replete nutrient conditions (micronutrients following F/2; 100 µmol L-1 nitrate, 100 µmol L-1 silicate, 6 µmol L-1 phosphate) in dilute batch cultures. Culture bottles were aerated with air containing a pCO2 of 400 µatm ensure a stabilized carbonate chemistry and to avoid sedimentation. After an acclimation period of >7 generation or >10 days, respectively, various functional traits were obtained: specific growth rate, quotas of particulate organic carbon (POC) and nitrogen (PON) as well as chlorophyll a (Chl a), photo-physiology using fast repetition rate fluorometry. Furthermore, the following parameters were calculated from the obtained data: POC:PON, Chl a:POC, POC production rate, photosystem II quantum yield, absolute electron transport rates at in-situ irradiance and maximal irradiance, light use efficiency, light acclimation indices, lifetime of PSII reopening and absorption cross section.
Comment:
Thalassiosira hylina strain KB3SS5, isolated 2020 in Kongsjford (Svalbard)
Micromonas pusilla strain CLP1, isolated 2014 in Kongsfjord (Svalbard)
Nitzschia frigida strain UIO-542, isolated in 2019 in the Fram strait
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Experiment | Exp | Rehder, Linda | |||
2 | Sampling date | Sampling date | Rehder, Linda | |||
3 | Sample ID | Sample ID | Rehder, Linda | |||
4 | Type of study | Study type | Rehder, Linda | |||
5 | Laboratory | Lab | Rehder, Linda | |||
6 | Species, unique identification | Species UID | Rehder, Linda | |||
7 | Species, unique identification (URI) | Species UID (URI) | Rehder, Linda | |||
8 | Strain | Strain | Rehder, Linda | |||
9 | Time in years | Time | a | Rehder, Linda | isolation year | |
10 | Location | Location | Rehder, Linda | isolation coordinates | ||
11 | Treatment: temperature | T:temp | °C | Rehder, Linda | ||
12 | Treatment: partial pressure of carbon dioxide | T:pCO2 | µatm | Rehder, Linda | ||
13 | Treatment: light:dark cycle | T:L:D | hh:hh | Rehder, Linda | ||
14 | Treatment: light intensity | T:Io | µmol/m2/s | Rehder, Linda | ||
15 | Treatment: salinity | T:sal | Rehder, Linda | |||
16 | Medium | Medium | Rehder, Linda | |||
17 | Generation | Generation | Rehder, Linda | # in acclimation | ||
18 | Growth rate | µ | 1/day | Rehder, Linda | Daily cell counting, Beckmann coulter Counter | |
19 | Carbon, organic, particulate, per cell | POC/cell | pg/# | Rehder, Linda | Flash combustions technique, CN Analyzer, EuroVector EA 3000 | |
20 | Nitrogen, organic, particulate, per cell | PON/cell | pg/# | Rehder, Linda | Flash combustions technique, CN Analyzer, EuroVector EA 3000 | |
21 | Carbon, organic, particulate/Nitrogen, organic, particulate ratio | POC/PON | mol/mol | Rehder, Linda | Calculated | |
22 | Chlorophyll a per cell | Chl a/cell | pg/# | Rehder, Linda | Fluorometry | including acidification step (Knap et al. 1996; https://doi.org/10.25607/obp-1409) |
23 | Chlorophyll a/particulate organic carbon ratio | Chl a/POC | pg/pg | Rehder, Linda | Calculated | |
24 | Carbon, organic, particulate, net production, per cell | NP POC/cell | pg/#/day | Rehder, Linda | Calculated | =µ*POC/cell |
25 | Absorption cross section of PSII photochemistry | nm**2/PSII | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | ||
26 | Time constant for the initial part of an single turnover relaxation phase | Tau | µs | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | |
27 | Quantum yield efficiency of photosystem II | Fv/Fm | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | ||
28 | Electron transport rate, absolute | aETR | e/PSII/s | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | abs ETR in-situ (e- PSII-1 s-1) |
29 | Light use efficiency | alpha | µmol/m2/s | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | |
30 | Light saturation | Ek | µmol/m2/s | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | |
31 | Maximal electron transport rate | ETR max | e/PSII/s | Rehder, Linda | Fast repetition rate fluorometry (FRRF) | abs ETR Vmax (e- PSII-1 s-1) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1866 data points