Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 GEOMAR - Helmholtz Centre for Ocean Research Kiel GEOMAR http://www.geomar.de https://ror.org/02h2x0161 Koch Florian florian.koch@awi.de 0000-0001-7107-4160 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 Trimborn Scarlett scarlett.trimborn@awi.de 0000-0003-1434-9927 Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven AWI https://www.awi.de/ https://ror.org/032e6b942 2024 The effect of light, iron and manganese supply on phytoplankton community composition in the Weddell Sea dataset https://doi.org/10.1594/PANGAEA.967722 2024-08-13T00:01:19 This study highlights the importance of manganese (Mn) next to iron (Fe) for the growth of specific Southern Ocean phytoplankton groups with important implications for primary production in the Weddell Sea. Two Fe-Mn-light bottle amendment experiment were performed with a natural phytoplankton community during Polarstern expedition PS124 in 2021 in the Weddell Sea. At this location, seawater was pumped (using trace metals clean techniques) from 25m depth to fill polycarbonate bottles. The Control treatment consisted of the sampled seawater without any trace metals addition, while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles under 2 light regimes (30 and 100 µmol photon m-2 s-1). After on average 7 days, samples for chlorophyll a content, particulate organic carbon, primary production flow cytometry and light microscopy were taken to detect FeMn co-limitation effect on species composition and primary production. While results showed with high confidence that both communities were Fe-limited, no evidence was found that low light provoked a stronger response to Fe supply. Nevertheless, under low light, microscopic analysis at both locations revealed Fe-Mn co-limitation of a key phytoplankton group: Fragilariopsis sp., which numerically dominated the diatom communities. Our findings highlight that in response to light limitation, some key species may suffer from Fe-Mn co-limitation with subsequent implications for the biological carbon pump. Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 Koch Florian florian.koch@awi.de 0000-0001-7107-4160 Flintrop Clara 0000-0002-5711-0521 Völkner Christian christian.voelkner@awi.de Iversen Morten Hvitfeldt Morten.Iversen@awi.de 0000-0002-5287-1110 Trimborn Scarlett scarlett.trimborn@awi.de 0000-0003-1434-9927 2023 Effect of iron and manganese supply on a natural phytoplankton community of the Weddell Sea and further consequence for carbon export dataset https://doi.org/10.1594/PANGAEA.957278 -30.38305 -30.00196 -76.09976 -70.41772 -30.174752499999997 -73.2581 2021-02-08T21:18:57 2021-02-28T21:39:53 -4488.8 -467.1 SPP1158 Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas https://gepris.dfg.de/gepris/projekt/5472008 Fachbereich Biologie, Universität Rostock http://www.uni-rostock.de https://ror.org/03zdwsf69 Deutsche Forschungsgemeinschaft, Bonn DFG http://www.dfg.de/en/ https://doi.org/10.13039/501100001659 404421353 Iron manganese co-limitation – a potential driver of Southern Ocean phytoplankton ecology https://gepris.dfg.de/gepris/projekt/404421353 German Research Foundation DFG http://www.dfg.de/en/ https://doi.org/10.13039/501100001659 5472008 Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas https://gepris.dfg.de/gepris/projekt/5472008 PS124_5-5 -70.41961 -30.00196 -4488.8 2021-02-08T21:18:57 -70.41772 -30.01135 -4487.3 2021-02-09T02:55:56 https://registry.o2a-data.de/items/3202 Weddell Sea PS124 GPF 19-2_039, COSMUS https://doi.org/10.48433/BzPM_0755_2021 Hellmer, Hartmut H 2021-02-04 2021-03-31 Stanley, Falkland Island (Malvinas) Stanley, Falkland Island (Malvinas) 20210055 hdl:10013/epic.e68274e3-816b-4505-bc7b-b1b926e1fbcc https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS124.png doi:10.1594/PANGAEA.933473 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 In situ pump ISP Responsible: C. Voelkner PS124_68-1 -76.09976 -30.30265 -467.1 2021-02-28T17:55:04 -76.09531 -30.38305 -470.9 2021-02-28T21:39:53 https://registry.o2a-data.de/items/3202 Weddell Sea PS124 GPF 19-2_039, COSMUS https://doi.org/10.48433/BzPM_0755_2021 Hellmer, Hartmut H 2021-02-04 2021-03-31 Stanley, Falkland Island (Malvinas) Stanley, Falkland Island (Malvinas) 20210055 hdl:10013/epic.e68274e3-816b-4505-bc7b-b1b926e1fbcc https://www.awi.de/fileadmin/user_upload/MET/PolarsternExpeditionMaps/PS124.png doi:10.1594/PANGAEA.933473 Polarstern https://doi.org/10.17815/jlsrf-3-163 DBLK 8013132 In situ pump ISP Responsible: Ch. Voelkner; SL=36m 391 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Event label Event Event Type of study Study type type Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 Study type Treatment: nutrients T:nutrients treatment Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 T:nutrients Treatment: light intensity T:Io µmol/m**2/s treatment Molar Flux Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 T:Io [µmol/m**2/s] Incubation duration Inc dur days Time T http://dbpedia.org/resource/Time Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 Inc dur [days] Size fraction Size fraction size http://purl.obolibrary.org/obo/PATO_0000117 Fraction Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 Size fraction Chlorophyll a Chl a µg/l hdl:10013/epic.32187.d001 chlorophyll a http://purl.obolibrary.org/obo/CHEBI_18230 Density http://dbpedia.org/resource/Density Measured Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 Chl a [µg/l] Carbon, organic, particulate, population yield POC pop yield µg/ml carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 organic particulate Density http://dbpedia.org/resource/Density Measured Balaguer Jenna jbalaguer@geomar.de 0000-0001-9330-2275 POC pop yield [µg/ml] CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable Diatom assemblage Primary production Trace metal Weddell Sea author29244 author31281 author54494 author57617 author65546 author83872 award2111 award5 basis1 campaign47412 curlevel30 event2968540 event2968562 funder20472 funder213 geocode1599 geocode1600 geocode1601 geocode8128 inst20147 inst32 inst480 license21 method11179 method420 param120848 param130782 param137706 param162273 param185520 param2136 param30323 pi83872 project4201 refds957278 term1073204 term1073594 term20356 term38338 term38477 term41013 term43792 term43975 term44029 term44034 term44419 term47694 term47873 term49506 term51598 term68213