Möller, Kristof; Tillmann, Urban; Krock, Bernd; Koch, Florian; Meunier, Cédric Léo (2024): Intracellular goniodomin A (GDA) content of Alexandrium pseudogonyaulax determined by LC/MS-MS analysis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.967418, In: Möller, K et al. (2024): Predator-prey interactions between three strains of the harmful dinoflagellate Alexandrium pseudogonyaulax and three life-stages of the calanoid copepod Acartia tonsa [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.967577
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Published: 2024-08-27 • DOI registered: 2024-09-25
Abstract:
The ingestion rates of three Acartia tonsa life-stages (N4-nauplii, C4-copepodites and adult copepods) feeding on three different strains of Alexandrium pseudogonyaulax, isolated from the Danish Limfjord, were determined by means of bottle incubation experiments. In the same experiment the intracellular goniodomin A (GDA) content of A. pseudogonyaulax was determined by LC/MS-MS analysis. This dataset includes the intracellular GDA contents in mass per cell and normalized to the molar carbon content. The preparation and execution of the experiments were carried out between May 1 and July 15, 2022 in the laboratories of the Alfred-Wegener-Institute, Helgoland, Germany.
Keyword(s):
Supplement to:
Möller, Kristof; Tillmann, Urban; Pöchhacker, Magdalena; Varga, Elisabeth; Krock, Bernd; Porreca, Francesco; Koch, Florian; Harris, Thomas M; Meunier, Cédric Léo (2024): Toxic effects of the emerging Alexandrium pseudogonyaulax (Dinophyceae) on multiple trophic levels of the pelagic food web. Harmful Algae, 102705, https://doi.org/10.1016/j.hal.2024.102705
Funding:
Helmholtz Association of German Research Centres (HGF), grant/award no. Changing-Earth_Subtopic_6.2: Adaptation of marine life: From genes to ecosystems
Coverage:
Latitude: 56.630000 * Longitude: 8.290000
Date/Time Start: 2020-08-17T09:10:00 * Date/Time End: 2020-08-17T09:10:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Möller, Kristof | Bottle incubation | ||
| 2 | Date/time start, experiment | Date/time start exp | Möller, Kristof | |||
| 3 | Date/time end, experiment | Date/time end exp | Möller, Kristof | |||
| 4 | Incubation duration | Inc dur | h | Calculated | ||
| 5 | Replicate | Repl | Möller, Kristof | |||
| 6 | Species, unique identification, predator | Species UID pred | ||||
| 7 | Species, unique identification, predator (URI) | Species UID pred (URI) | ||||
| 8 | Species, unique identification, predator (Semantic URI) | Species UID pred (Semantic URI) | ||||
| 9 | Treatment: predator, life stage | T:predator LS | Möller, Kristof | Visual identification (VID) of Lugol preserved samples | N4: naupliar stage 4; C4: copepodid stage 4; Cop: adult copepods; C: Control (no predators) | |
| 10 | Species, unique identification, prey | Species UID prey | ||||
| 11 | Species, unique identification, prey (URI) | Species UID prey (URI) | ||||
| 12 | Species, unique identification, prey (Semantic URI) | Species UID prey (Semantic URI) | ||||
| 13 | Treatment: prey | T:prey | Möller, Kristof | Strains of Alexandrium pseudogonyaulax as prey | ||
| 14 | Alexandrium pseudogonyaulax, strain | A. pseudogonyaulax strain | Möller, Kristof | |||
| 15 | Alexandrium pseudogonyaulax, goniodomin A per cell | A. pseudogonyaulax GDA/cell | pg/# | Möller, Kristof | Centrifuged off, solubilized in methanol, spin-filtered; measured with ultra-high-performance liquid chromatography (UHPLC); coupled with triple quadrupole mass spectrometer (TQMS), Waters, Xevo TQ-XS | Toxin content |
| 16 | Alexandrium pseudogonyaulax, goniodomin A/carbon ratio | A. pseudogonyaulax GDA/C | mol/mol | Möller, Kristof | Centrifuged off, solubilized in methanol, spin-filtered; measured with ultra-high-performance liquid chromatography (UHPLC); coupled with triple quadrupole mass spectrometer (TQMS), Waters, Xevo TQ-XS | Toxin content |
| 17 | Alexandrium pseudogonyaulax, carbon, organic, particulate, per cell | A. pseudogonyaulax POC/cell | ng/# | Möller, Kristof | Kept in dark at -20°C on precombusted Gf/f filter; dried for 24 h at 60 °C, then added conc. HCl and dried for 24 h at 60 °C; measured with Elemental analyser, HEKAtech, Euro EA 3000 | |
| 18 | Alexandrium pseudogonyaulax, nitrogen, organic, particulate, per cell | A. pseudogonyaulax PON/cell | ng/# | Möller, Kristof | Kept in dark at -20°C on precombusted Gf/f filter; dried for 24 h at 60 °C, then added conc. HCl and dried for 24 h at 60 °C; measured with Elemental analyser, HEKAtech, Euro EA 3000 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1120 data points