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Lasbleiz, Marine; Bosc, Sébastien; Richier, Sophie (2024): Laboratory experiments of nitrate uptake kinetic of the green tide reference algae Ulva spp. during two successive productive seasons (2022-2023) observed on Brittany coasts [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.967049

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Published: 2024-04-02 • DOI registered: 2024-05-01

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Abstract:
The seasonal proliferations of green seaweeds under the morphological blade-type Ulva have been observed on Brittany coasts since the late 60s-early 70s. These so-called green tides are directly related to the eutrophication of coastal waters, and especially to high terrigenous inputs of nitrogen to the sea. In this context, a management tool, i.e. the ecological Model for Application at Regional Scale MARS3D-Ulve, has specifically been developed to support stakeholders to set realistic targets for nitrogen input reductions. Among key parameters of the model, the maximum nitrate uptake rate (Vmax) and the half-saturation constant for nitrate (Km) of Ulva sp. have initially been calibrated to reproduce the seasonal variation of the internal nitrogen and phosphorus algal contents measured in situ for five sites along the Brittany coastline (La Fresnaye Bay, Saint-Brieuc Bay, Lannion Bay, Guissény Bay and Douarnenez Bay; Perrot et al. (2014, doi:10.1016/j.jmarsys.2013.12.010)). The calibrated values of Vmax and Km require therefore to be experimentally validated.
Laboratory experiments were monthly set up to assess the seasonal evolution of nitrate uptake kinetic of Ulva spp. from May to September during two successive seasons (2022 and 2023). Healthy vegetative thalli of the dominant Ulva species were randomly sampled on Lermot Beach, located in Saint-Brieuc Bay (lat 48.5339, long 2.6622). Eventhough the model MARS3D-Ulve does not differentiate Ulva sp. at the species level, morpho-anatomical identification of the sampled thalli was carried out following the updated taxonomic classification of Hughey et al. (2021, doi:10.1080/09670262.2021.1914862). Algae were grown in 5 l polypropylen beakers in 0.2 µm filtered seawater under 7 different nitrate concentrations (0, 5, 10, 20, 50, 100, 200 µmol/l). Phosphate was added (3 µmol/l) in each beaker to avoid limitation. The experiment was run under a light intensity of 150 µmol photons m²/s. Photoperiod and temperature were adjusted to measured in situ conditions. The laboratory experiments consisted in assessing nitrates concentrations in each medium after 30 minute-incubation period. Nitrate uptake rates were calculated from nitrate concentrations and the corresponding kinetic parameters Vmax and Km were estimated by fitting data with the Michaelis-Menten equation, using a nonlinear least-squares regression (Lotze and Schramm, 2000, doi:10.1046/j.1529-8817.2000.99109.x). Each experiment was replicated three times. Before each experiment, physiological and nutritional states of algae were determined from the assessment of their maximum quantum yield (Fv/Fm), and their nitrogen and phosphorus tissue contents respectively. Those assessments were done from four replicated samples.
Coverage:
Latitude: 48.533900 * Longitude: 2.662200
Event(s):
Ulva_species_Lermot_Beach * Latitude: 48.533900 * Longitude: 2.662200 * Location: Brittany, France * Method/Device: Laboratory experiment
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 ExperimentExpLasbleiz, Marine
2 Date/time analysis, experimentDate/time analysis expLasbleiz, Marine
3 Species, unique identificationSpecies UIDLasbleiz, Marine
4 Species, unique identification (Semantic URI)Species UID (Semantic URI)Lasbleiz, Marine
5 Species, unique identification (URI)Species UID (URI)Lasbleiz, Marine
6 Treatment: temperatureT:temp°CLasbleiz, MarineWTW Multiline P4 analyserSeawater temperature
7 Light:Dark cycleL:Dhh:hhLasbleiz, MarinePhotoperiod set up during the experiment
8 Maximum quantum yield of photosystem IIFv/FmLasbleiz, MarineDiving-PAM-II photosynthesis system, WalzMeasured on dark-acclimated algae few minutes before the start of the ligh period. Measurement was replicated four times.
9 Maximum quantum yield of photosystem II, standard deviationFv/Fm std dev±Lasbleiz, MarineDiving-PAM-II photosynthesis system, Walz
10 Nitrate, maximum uptake rate per hour as dry massNO3 Vmax dmµmol/g/hLasbleiz, MarineCalculated, according to the Michaelis-Menten kineticAssessed by fitting data (nitrate uptake rates plotted function of the initial nitrate concentration in the media) with the Michaelis-Menten equation, using a nonlinear least-squares regression, as described in Lotze and Schramm (2000, doi:10.1046/j.1529-8817.2000.99109.x). Estimated from three replicates.
11 Nitrate, maximum uptake rate per hour as dry mass, standard deviation per hourNO3 Vmax dm±Lasbleiz, MarineCalculated, according to the Michaelis-Menten kinetic
12 Nitrate, half saturation constantNO3 Kµmol/lLasbleiz, MarineCalculated, according to the Michaelis-Menten kineticAssessed by fitting data (nitrate uptake rates plotted function of the initial nitrate concentration in the media) with the Michaelis-Menten equation, using a nonlinear least-squares regression, as described in Lotze and Schramm (2000, doi:10.1046/j.1529-8817.2000.99109.x). Estimated from three replicates.
13 Nitrate, half saturation constant, standard deviationNO3 K std dev±Lasbleiz, MarineCalculated, according to the Michaelis-Menten kinetic
14 Nitrogen, in tissue, per dry massN/dm tisg/100 gLasbleiz, MarineNitrogen analysis after DumasQuantified from the Dumas method, described in Marcó et al. (2002, doi:10.1016/s0039-9140(02)00136-4).Estimated from four algae samples of 0.5 to 1 g dry weight (dw).
15 Nitrogen, in tissue, per dry mass, standard deviationN/dm tis std dev±Lasbleiz, MarineNitrogen analysis after Dumas
16 Phosphorus, in tissue, per dry massP/dm tisg/100 gLasbleiz, MarineInductively coupled plasma atomic emission spectroscopy (ICP-AES)Quantified by inductively coupled plasma atomic emission spectroscopy (ICP-AES) after mineralization step. Estimated from four algae samples of 0.5 to 1 g dry weight (dw).
17 Phosphorus, in tissue, per dry mass, standard deviationP/dm tis±Lasbleiz, MarineInductively coupled plasma atomic emission spectroscopy (ICP-AES)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
150 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Exp		2 
Date/time analysis exp		3 
Species UID		4 
Species UID (Semantic URI)		5 
Species UID (URI)		6 
T:temp [°C]		7 
L:D [hh:hh]		8 
Fv/Fm		9 
Fv/Fm std dev [±]		10 
NO3 Vmax dm [µmol/g/h]		11 
NO3 Vmax dm [±]		12 
NO3 K [µmol/l]		13 
NO3 K std dev [±]		14 
N/dm tis [g/100 g]		15 
N/dm tis std dev [±]		16 
P/dm tis [g/100 g]		17 
P/dm tis [±]
Laboratory experiment2022-05-17T12:15:01Ulva lacinulataurn:lsid:marinespecies.org:taxname:1360911marinespecies.org16.015:90.7430.043207.050.735.017.71.290.070.1500.008
Laboratory experiment2022-06-13T11:38:00Ulva lacinulataurn:lsid:marinespecies.org:taxname:1360911marinespecies.org18.016:80.6720.026223.729.939.914.11.340.070.2060.002
Laboratory experiment2022-07-04T12:00:00Ulva lacinulataurn:lsid:marinespecies.org:taxname:1360911marinespecies.org19.016:80.6910.021202.510.927.61.71.430.090.2650.019
Laboratory experiment2022-08-08T12:35:00Ulva lacinulataurn:lsid:marinespecies.org:taxname:1360911marinespecies.org19.015:90.6440.030156.810.223.65.31.230.030.4520.037
Laboratory experiment2022-09-12T12:05:00Ulva rotundataurn:lsid:marinespecies.org:taxname:145991marinespecies.org19.013:110.7220.011253.221.026.17.31.300.090.1770.005
Laboratory experiment2023-05-04T11:56:00Ulva lacinulataurn:lsid:marinespecies.org:taxname:1360911marinespecies.org16.015:90.7770.041115.425.323.67.23.100.120.1280.012
Laboratory experiment2023-06-08T12:07:00Ulva lacinulataurn:lsid:marinespecies.org:taxname:1360911marinespecies.org18.516:80.7110.036157.916.422.56.11.800.090.1810.011
Laboratory experiment2023-07-12T11:36:00Ulva lacinulataurn:lsid:marinespecies.org:taxname:1360911marinespecies.org19.516:80.7610.020221.823.145.37.31.320.050.2490.031
Laboratory experiment2023-08-09T12:08:00Ulva rotundataurn:lsid:marinespecies.org:taxname:145991marinespecies.org18.015:90.7710.002105.35.018.22.32.220.110.2300.007
Laboratory experiment2023-09-07T11:46:00Ulva rotundataurn:lsid:marinespecies.org:taxname:145991marinespecies.org20.813:110.6570.034137.510.227.02.61.110.040.1770.007