Martins, Ines; Goulart, Joana; Godinho, Antonio; Mano, Beatriz; Sire de Vilar, Anaïs; Carreiro-Silva, Marina (2024): Cellular stress biomarkers result of the cold-water coral Dentomuricea aff. meteor during a land-based experiment testing the cumulative effect of Cu exposure and ocean acidification [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.966792 (DOI registration in progress)
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Abstract:
We report the results of a land-based experiment testing the cumulative effects of copper (Cu) exposure and ocean acidification (OA). Corals were obtained as by-catch during experimental long-line fisheries campaigns on board R/V "Arquipélago" (ARQDAÇO monitoring program, University of the Azores) at Baixa de São Mateus at 185 m depth in April 2019. The experiment was undertaken at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores) where corals were exposed to four OA/Cu-contamination scenarios: (1) ambient pCO2/pH level as measured in situ conditions (385 μatm/ pH 8.09); (2) high pCO2/reduced pH (IPCC RCP8.5 scenario, 1000 μatm/ pH 7.73); (3) ambient pCO2/pH level and additional Cu concentration (60 µg/l); (4) high pCO2/reduced pH and additional Cu concentration (60 µg/l). The pH/pCO2 modification was achieved by bubbling seawater with either pure CO2 (to increase pCO2) or CO2 low air (to decrease pCO2). The copper concentration used in this experiment followed a trial simulating a polymetallic particles plume release during a potential deep-sea mining event. This sublethal concentration was found to be the highest copper concentration dissolved in seawater.
Metabolic (CA and Hsp70), oxidative damage (SOD, MDA) and detoxification (GST) biomarkers were measured in 4 corals from each treatment at the end of the experiment, using a multiplate spectrophotometer reader.
Project(s):
Funding:
European Commission (EC), grant/award no. ACORES-01-0145-FEDER-000140: InvestigadoresMarAZ-Consolidar corpo de investigadores em Ciências do Mar nos Açores
Horizon 2020 (H2020), grant/award no. 818123: Integrated Assessment of Atlantic Marine Ecosystems in Space and Time
Coverage:
Median Latitude: 38.575000 * Median Longitude: -28.676665 * South-bound Latitude: 38.250000 * West-bound Longitude: -29.083330 * North-bound Latitude: 38.900000 * East-bound Longitude: -28.270000
Date/Time Start: 2019-04-30T00:00:00 * Date/Time End: 2019-10-10T00:00:00
Minimum Elevation: -200.0 m * Maximum Elevation: -200.0 m
Event(s):
Azores_Deep_Octocoral_Sampling_Viminella_flagellum * Latitude: 38.900000 * Longitude: -29.083330 * Date/Time: 2019-10-10T00:00:00 * Elevation: -200.0 m * Location: Condor Seamounts
Dentomuricea_aff_meteor_Aquaria_Experiment_1 * Latitude: 38.250000 * Longitude: -28.270000 * Date/Time: 2019-04-30T00:00:00 * Location: Azores * Method/Device: Laboratory experiment * Comment: Experiment conducted at the DeepSeaLab aquaria facilities of the Okeanos Marine Institute, University of the Azores. Species: octocoral Dentomuricea aff. meteor
Comment:
Species: octocoral Dentomuricea aff. meteor
1. Experimental treatments
Treatments
8.1 + [0]: ambient pH plus no copper addition
8.1 + [60]: ambient pH plus Cu concentration of 60 μg/l
7.7 + [0]: low pH plus no copper addition
7.7 + [60]: low pH plus Cu concentration of 60 μg/l
Experiment duration: 9 days
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Martins, Ines | |||
| 2 | Species | Species | Martins, Ines | Species affinis | ||
| 3 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Martins, Ines | |||
| 4 | Species, unique identification (URI) | Species UID (URI) | Martins, Ines | |||
| 5 | Treatment | Treat | Martins, Ines | Treatment: pH + [Cu] µg/l (solution prepared with Certipur® Cu(NO3)2 Merck KGaA©) | ||
| 6 | Time in days | Time | days | Martins, Ines | ||
| 7 | Aquaria ID | Aquaria ID | Martins, Ines | |||
| 8 | Colony number/ID | Colony no/ID | Martins, Ines | Coral colony | ||
| 9 | Glutathione S-transferase, activity, per protein mass | GTS/prot | mmol/min/mg | Martins, Ines | Multiskan Microplate spectrophotometer, Thermo Scientific™, GO | |
| 10 | Superoxide dismutase activity, unit per protein mass | SOD/prot | U/mg | Martins, Ines | Multiskan Microplate spectrophotometer, Thermo Scientific™, GO | |
| 11 | Carbonic anhydrase activity, per protein | CA act/prot | U/g | Martins, Ines | Multiskan Microplate spectrophotometer, Thermo Scientific™, GO | |
| 12 | Heat shock protein 70, per protein mass | HPS70/prot | µg/mg | Martins, Ines | Multiskan Microplate spectrophotometer, Thermo Scientific™, GO | |
| 13 | Malondialdehyde, per protein mass | MDA/prot | nmol/mg | Martins, Ines | Multiskan Microplate spectrophotometer, Thermo Scientific™, GO |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
640 data points