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Gutiérrez-Zárate, Cristina; Veiga, Alfredo; Gori, Andrea; Gerpe, Fabián; Domínguez, Leo; Rodríguez, Alberto; Arias, Noelia; Álvarez, Marta; Acerbi, Rubén; Vázquez, Lucía; Movilla, Juancho; Varela, Marta M; Alba-Salgueiro, Rodrigo; Valencia-Vila, Joaquín; Orejas, Covadonga (2024): Prokariotic abundances during a multi-stressor aquaria experiment with the cold-water coral Dendrophyllia cornigera [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.966611

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Abstract:
A 9-month aquarium experiment with the cold-water Dendrophyllia cornigera was conducted to investigate the single and combined effects of warming, acidification and deoxygenation on its ecophysiological response. The experiment took place at the Aquarium finisterrae (A Coruña, Spain) between 2022-05-06 and 2023-02-24. Treatment values for each parameter (current in situ vs. climate change) were: 12 °C and 15 °C (temperature); ~7.99 and 7.69 (pH); ~8.63 mg/L and 6.45 mg/L (dissolved oxygen concentration). A total of eight treatments (with 3 replicates each, 5 L aquaria) were set up. Prokaryotes were quantified by flow citometry (CytoFLEXflex S, Beckman Coulter) as previously described by Gasol et al. (1999) with our own adjustment of the scatter settings to more accurately resolve these cells with the new CytoFLEX flow cytometry technology. Measurements were performed every month from different parts of the aquaria setup: the input seawater, the reservoir tank (after the filtration of seawater to 5 µm and before the UV lamp) and every experimental aquaria.
Keyword(s):
flow cytometry; Laboratory experiment; NE Atlantic; NW Spain; prokaryotic abundance
Related to:
Gutiérrez-Zárate, Cristina; Gori, Andrea; Veiga, Alfredo; Álvarez, Marta; Acerbi, Rubén; Vázquez, Lucía; Varela, Marta M; Alba-Salgueiro, Rodrigo; Valencia-Vila, Joaquín; Orejas, Covadonga (2024): Respiration rates of the cold-water coral Dendrophyllia cornigera from a long-term multi-stressor experiment. PANGAEA, https://doi.org/10.1594/PANGAEA.966748
Gutiérrez-Zárate, Cristina; Gori, Andrea; Veiga, Alfredo; Álvarez, Marta; Acerbi, Rubén; Vázquez, Lucía; Varela, Marta M; Alba-Salgueiro, Rodrigo; Valencia-Vila, Joaquín; Orejas, Covadonga (2024): Skeletal growth rates of the cold-water coral Dendrophyllia cornigera from a long-term multi-stressor experiment. PANGAEA, https://doi.org/10.1594/PANGAEA.966744
References:
Gasol, Josep M; Zweifel, U L; Peters, F; Fuhrman, Jed A; Hagström, Åke (1999): Significance of Size and Nucleic Acid Content Heterogeneity as Measured by Flow Cytometry in Natural Planktonic Bacteria. Applied and Environmental Microbiology, 65(10), 4475-4483, https://doi.org/10.1128/AEM.65.10.4475-4483.1999
Funding:
Horizon 2020 (H2020), grant/award no. 818123: Integrated Assessment of Atlantic Marine Ecosystems in Space and Time
Coverage:
Latitude: 43.398822 * Longitude: -8.700804
Date/Time Start: 2022-07-13T00:00:00 * Date/Time End: 2023-02-23T00:00:00
Minimum Elevation: -150.0 m * Maximum Elevation: -150.0 m
Event(s):
Dendrophyllia_Bycatch * Latitude: 43.398822 * Longitude: -8.700804 * Date/Time: 2019-09-11T00:00:00 * Elevation: -150.0 m * Location: Atlantic Ocean
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeGutiérrez-Zárate, Cristina
2DATE/TIMEDate/TimeGutiérrez-Zárate, CristinaGeocode
3SpeciesSpeciesGutiérrez-Zárate, Cristina
4Species, unique identification (URI)Species UID (URI)Gutiérrez-Zárate, Cristina
5Species, unique identification (Semantic URI)Species UID (Semantic URI)Gutiérrez-Zárate, Cristina
6Experiment durationExp durationdaysGutiérrez-Zárate, Cristina
7Experimental treatmentExp treatGutiérrez-Zárate, Cristina
8ReplicateReplGutiérrez-Zárate, Cristina
9ProkaryotesProkaryotes#/mlGutiérrez-Zárate, CristinaFlow cytometer, Beckman Coulter, CytoFLEX S (N-V-B-R)
10CommentCommentGutiérrez-Zárate, Cristina
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
1260 data points

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