Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 Chang William Weijen w.chang@physiologie.uni-kiel.de 0000-0002-9477-495X Hu Marian Y m.hu@physiologie.uni-kiel.de 0000-0002-8914-139X Stumpp Meike mstumpp@zoologie.uni-kiel.de 0000-0001-7765-2996 2024 Total protease activity in larval Strongylocentrotus purpuratus during laboratory experiments with soybean trypsin inhibitor (SBTI) treatment over time dataset https://doi.org/10.1594/PANGAEA.965982 2024-04-18T07:35:47 https://doi.org/10.1594/PANGAEA.966001 Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology. Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 Chang William Weijen w.chang@physiologie.uni-kiel.de 0000-0002-9477-495X Hu Marian Y m.hu@physiologie.uni-kiel.de 0000-0002-8914-139X Stumpp Meike mstumpp@zoologie.uni-kiel.de 0000-0001-7765-2996 2023 Characterization of digestive proteases in the gut of a basal deuterostome journal article Journal of Experimental Biology 226(15) https://doi.org/10.1242/jeb.245789 jeb245789 -117.257767 -117.257767 32.842674 32.842674 -117.257767 32.842674 uncoded table German Research Foundation DFG http://www.dfg.de/en/ https://doi.org/10.13039/501100001659 441084746 Black box larval physiology - mechanisms of nutrient acquisition and energetics of invertebrate larvae in a changing ocean https://gepris.dfg.de/gepris/projekt/441084746 La_Jolla_S_purpuratus 32.842674 -117.257767 La Jolla, USA Sampling by diver DIVER Strongylocentrotus purpuratus originally collected in November 2020 48 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Determination of the required pre-incubation time for the maximum efficacy of the soybean trypsin inhibitor when using in the total protease activity measurements. ----- The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2. Experiment Exp Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 ID Exp Sampling date/time, experiment Date/time sampling exp Time T http://dbpedia.org/resource/Time Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 Date/time sampling exp Sample ID Sample ID Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 Sample ID Type of study Study type type Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 Study type Laboratory Lab Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 Lab Time in minutes Time min min Time T http://dbpedia.org/resource/Time Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 Incubation time Time min [min] Treatment: soybean trypsin inhibitor T:SBTI µg/ml treatment Density http://dbpedia.org/resource/Density Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 T:SBTI [µg/ml] Strongylocentrotus purpuratus, larvae, total protease activity, difference in absorption per time and protein mass S. purpuratus larv TPA 1/h/mg Strongylocentrotus purpuratus https://www.marinespecies.org/aphia.php?p=taxdetails&id=240747 larvae Calculated Hildebrand Jasper jhildebrand@zoologie.uni-kiel.de 0000-0002-7467-6236 Absorbance measured with BioSpectrometer (Eppendorf, Hamburg, Germany) at 366 nm. Mean (n=3). S. purpuratus larv TPA [1/h/mg] CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable algae culture experiment energetics Laboratory experiment physiology protein sea urchin Strongylocentrotus purpuratus author104593 author40482 author40512 author86039 award2886 curlevel30 event3121830 funder20472 geocode1600 geocode1601 journal8691 license21 method11565 method50 param16065 param185520 param20622 param5058 param516121 param51998 param527590 param527592 pi104593 ref124704 term1054753 term38841 term41013 term41036 term43224 term43792 term43975 term49506 term49792 term50012 term50087 term54214 term60989 term70413 term73770 topotype11