10.1594/PANGAEA.965982Hildebrand, JasperJasperHildebrand0000-0002-7467-6236Chang, William WeijenWilliam WeijenChang0000-0002-9477-495XHu, Marian YMarian YHu0000-0002-8914-139XStumpp, MeikeMeikeStumpp0000-0001-7765-2996PANGAEA2024algaeculture experimentenergeticsLaboratory experimentphysiologyproteinsea urchinStrongylocentrotus purpuratusExperimentSampling date/time, experimentSample IDType of studyLaboratoryTime in minutesTreatment: soybean trypsin inhibitorStrongylocentrotus purpuratus, larvae, total protease activity, difference in absorption per time and protein massSampling by diverCalculatedDataset10.1594/PANGAEA.96600110.1242/jeb.24578948 data pointstext/tab-separated-valuesCreative Commons Attribution 4.0 InternationalStrongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.Determination of the required pre-incubation time for the maximum efficacy of the soybean trypsin inhibitor when using in the total protease activity measurements.
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The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between January and June 2022 as part of the publication Characterization of digestive proteases in the gut of a basal deuterostome. Larvae of cultured S. purpuratus from the GEOMAR Helmholtz Centre for Ocean Research Kiel, were cultured for up to 14 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.-117.25776732.842674La Jolla, USAGerman Research Foundationhttps://doi.org/10.13039/501100001659441084746Black box larval physiology - mechanisms of nutrient acquisition and energetics of invertebrate larvae in a changing ocean