Schauberger, Clemens; Middelboe, Mathias; Glud, Ronnie N (2024): Quantification of microbial cells and virus-like particles in sediments from Atacama Trench [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.965920
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Published: 2024-03-04 • DOI registered: 2024-04-02
Abstract:
Sediment cores were collected using a multicorer (MUC). Microbes and virus-like particles were extracted from sediments in a 3°C room using a modified version of the washing protocol of Danovaro and Middelboe, 2010 (see Schauberger et al., 2021). After the washing procedure, the extracted microbial cells and virus-like particles were fixed with 25% glutaraldehyde (1% final concentration) and stored at −80°C prior to flow cytometry. These samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I. Sediment extracts were diluted 1 : 10 in 0.02 μm-filtered TE Buffer prior to all measurements. The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).
Keyword(s):
Related to:
Schauberger, Clemens; Middelboe, Mathias; Larsen, Morten; Peoples, Logan M; Bartlett, Douglas H; Kirpekar, Finn; Rowden, Ashley A; Wenzhöfer, Frank; Thamdrup, Bo; Glud, Ronnie N (2021): Spatial variability of prokaryotic and viral abundances in the Kermadec and Atacama Trench regions. Limnology and Oceanography, 66(6), 2095-2109, https://doi.org/10.1002/lno.11711
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Median Latitude: -22.643689 * Median Longitude: -71.265900 * South-bound Latitude: -24.285000 * West-bound Longitude: -71.620700 * North-bound Latitude: -20.326200 * East-bound Longitude: -70.838050
Date/Time Start: 2018-03-06T07:41:00 * Date/Time End: 2018-03-28T17:17:00
Minimum DEPTH, sediment/rock: 0.0050 m * Maximum DEPTH, sediment/rock: 0.3250 m
Event(s):
SO261_9-1 (Site 1) * Latitude: -23.820833 * Longitude: -70.838050 * Date/Time: 2018-03-06T07:41:00 * Elevation: -2544.6 m * Campaign: SO261 * Basis: Sonne_2 * Method/Device: MultiCorer (MUC)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Station label | Station | Schauberger, Clemens | |||
| 3 | Latitude of event | Latitude | ||||
| 4 | Longitude of event | Longitude | ||||
| 5 | Date/Time of event | Date/Time | ||||
| 6 | Elevation of event | Elevation | m | |||
| 7 | Depth, top/min | Depth top | m | Schauberger, Clemens | ||
| 8 | Depth, bottom/max | Depth bot | m | Schauberger, Clemens | ||
| 9 | DEPTH, sediment/rock | Depth sed | m | Schauberger, Clemens | Geocode | |
| 10 | Microbial abundance, cells | Microbial abund | #/ml | Schauberger, Clemens | Flow cytometry system, Becton Dickinson, FACSCanto II | |
| 11 | Microbial abundance, standard deviation | Microbial abund std dev | ± | Schauberger, Clemens | ||
| 12 | Viral abundance | Virus | #/ml | Schauberger, Clemens | Flow cytometry system, Becton Dickinson, FACSCanto II | |
| 13 | Viral abundance, standard deviation | Virus std dev | ± | Schauberger, Clemens | ||
| 14 | Carbon, organic, total | TOC | % | Schauberger, Clemens |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2167 data points
Download Data
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