Khalil, Munawar; Stuhr, Marleen; Kunzmann, Andreas; Westphal, Hildegard: Total lipids of the tropical-subtropical starfish Aquilonastra yairi under ocean acidification and warming in the laboratory [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.965904 (dataset in review)
Abstract:
Total lipids were studied in the tropical-subtropical starfish Aquilonastra yairi from the culture stock of the ZMT-MAREE laboratory in response to projected near-future global change. To investigate the physicochemical alteration of starfish induced by elevated seawater pCO2 and temperature, starfish of the species A. yairi (342 specimens, size 3-11 mm in diameter, culture stock) were reared in aquaria system under controlled experimental environment conditions at ZMT-MAREE. The starfish were acclimatized to two temperature levels (27 °C, 32 °C) crossed with three pCO2 concentrations (455 µatm, 1052 µatm, 2066 µatm). Total lipids were determined following Bligh and Dyer (1959). Total lipids were unaffected by combined elevated temperature and pCO2, but at elevated temperature, there was an increase in total lipids.
References:
Bligh, E G; Dyer, W J (1959): A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911-917, https://doi.org/10.1139/o59-099
Dickson, Andrew G; Sabine, Christopher L; Christian, J R (2007): Guide to Best Practices for Ocean CO2 Measurements. PICES Special Publication, 3, 191 pp, hdl:10013/epic.51789.d001
Pierrot, D E; Lewis, E; Wallace, D W R (2006): MS Excel Program Developed for CO2 System Calculations. Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy, https://doi.org/10.3334/CDIAC/otg.CO2SYS_XLS_CDIAC105a
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Khalil, Munawar | |||
| 2 | Species, unique identification | Species UID | Khalil, Munawar | |||
| 3 | Species, unique identification (URI) | Species UID (URI) | Khalil, Munawar | |||
| 4 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Khalil, Munawar | |||
| 5 | Treatment | Treat | Khalil, Munawar | |||
| 6 | Sampling date/time, experiment | Date/time sampling exp | Khalil, Munawar | |||
| 7 | Treatment: temperature | T:temp | °C | Khalil, Munawar | Multi-parameter portable meter, Xylem Analytics, WTW MultiLine® 3630 IDS | |
| 8 | Treatment: partial pressure of carbon dioxide | T:pCO2 | µatm | Khalil, Munawar | calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006) | |
| 9 | pH | pH | Khalil, Munawar | Spectrophotometer; according to Dickson et al. (2007) | ||
| 10 | pH, standard deviation | pH std dev | ± | Khalil, Munawar | Calculated | |
| 11 | Individual ID | Ind ID | Khalil, Munawar | |||
| 12 | Lipids, per unit dry mass | Lipid/dm | mg/g | Khalil, Munawar | Extraction of Lipids after Bligh & Dyer (1959) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
510 data points