Citation:

PANGAEA.965883

Name: Seawater carbonate chemistry and diatom aggregation when exposed to crude oil and chemical dispersant
Published: 2024-02-27
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bacillariophyta; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Laboratory experiment; Laboratory strains; Not applicable; Organic toxins; Other studied parameter or process; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

Roller table experiments with Thalassiosira pseudonana, a small centric diatom, were conducted to produce marine snow aggregates in six treatments: Control (pCO2 = 400 ppm), OA (pCO2 = 750 ppm), water accommodated fraction of oil (WAF), OAWAF, diluted chemically enhanced WAF (DCEWAF), and OADCEWAF. Measurements included intracellular photophysiological responses, oil concentrations and polycyclic aromatic hydrocarbons (PAHs), aggregate morphology, transparent exopolymeric particles (TEP), and extracellular polymeric substances (EPS) to investigate if OA will affect the response to oil spill conditions. The experiments were conducted in the dark to eliminate cell replication and photosynthesis and used both stationary and exponential growth phases.

Keyword(s):

Bacillariophyta; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Laboratory experiment; Laboratory strains; Not applicable; Organic toxins; Other studied parameter or process; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Supplement to:

Genzer, Jennifer L; Kamalanathan, Manoj; Bretherton, Laura; Hillhouse, Jessica; Xu, Chen; Santschi, Peter H; Quigg, Antonietta (2020): Diatom aggregation when exposed to crude oil and chemical dispersant: Potential impacts of ocean acidification. PLoS ONE, 15(7), e0235473, https://doi.org/10.1371/journal.pone.0235473

Source:

Genzer, Jennifer L; Hillhouse, Jessica; Kamalanathan, Manoj; Bretherton, Laura; Quigg, Antonietta (2020): Potential impacts of ocean acidification on diatom aggregation when exposed to crude oil and chemical dispersants. Harte Research Institute, https://doi.org/10.7266/VF86SWMX

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2019): seacarb: Seawater Carbonate Chemistry. R package version 3.2.15. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-01-26.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Quigg, Antonietta
2	Species, unique identification	Species UID		Quigg, Antonietta
3	Species, unique identification (URI)	Species UID (URI)		Quigg, Antonietta
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Quigg, Antonietta
5	Time point, descriptive	Time point		Quigg, Antonietta
6	Growth phase	Growth phase		Quigg, Antonietta
7	Treatment	Treat		Quigg, Antonietta		OA=ocean acidification, WAF=water accommodated fraction of oil, DCEWAF=dilute chemically enhanced water accommodated fraction of oil
8	Relative fluorescence intensity, ratio	Q		Quigg, Antonietta
9	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Quigg, Antonietta
10	Connectivity between photosystem II	p		Quigg, Antonietta
11	Chlorophyll a	Chl a	µg/l	Quigg, Antonietta		surrounding seawater
12	Chlorophyll a	Chl a	µg/l	Quigg, Antonietta		aggregate slurry
13	Cell density	Cells	#/ml	Quigg, Antonietta
14	Carbohydrates mass	CHO	mg	Quigg, Antonietta		surrounding seawater
15	Proteins	Protein	µg	Quigg, Antonietta		surrounding seawater
16	Carbohydrates mass	CHO	mg	Quigg, Antonietta		aggregate slurry
17	Proteins	Protein	µg	Quigg, Antonietta		aggregate slurry
18	Transparent exopolymer particles as Gum Xanthan equivalents per volume	TEP	µg Xeq/l	Quigg, Antonietta
19	Polycyclic aromatic hydrocarbons	PAH	mg/l	Quigg, Antonietta		surrounding seawater
20	Polycyclic aromatic hydrocarbons, mass	PAH mass	mg	Quigg, Antonietta		surrounding seawater
21	Polycyclic aromatic hydrocarbons, mass	PAH mass	mg	Quigg, Antonietta		aggregate slurry
22	Equivalent spherical diameter	ESD	mm	Quigg, Antonietta		Large
23	Equivalent spherical diameter	ESD	mm	Quigg, Antonietta		Medium
24	Equivalent spherical diameter	ESD	mm	Quigg, Antonietta		Small
25	Equivalent spherical diameter	ESD	mm	Quigg, Antonietta		XS
26	Oil equivalent	Oil eq	mg/l	Quigg, Antonietta		Estimate
27	pH	pH		Quigg, Antonietta		total scale
28	Alkalinity, total	AT	µmol/kg	Quigg, Antonietta
29	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Quigg, Antonietta
30	Carbon, inorganic, dissolved	DIC	µmol/kg	Quigg, Antonietta
31	Bicarbonate ion	[HCO3]-	µmol/kg	Quigg, Antonietta
32	Carbonate ion	[CO3]2-	µmol/kg	Quigg, Antonietta
33	Carbon dioxide	CO2	µmol/kg	Quigg, Antonietta
34	Area	Area	cm²	Quigg, Antonietta		aggregate slurry, large
35	Area	Area	cm²	Quigg, Antonietta		aggregate slurry, medium
36	Area	Area	cm²	Quigg, Antonietta		aggregate slurry, small
37	Area	Area	cm²	Quigg, Antonietta		aggregate slurry, XS
38	Counts	Counts	#	Quigg, Antonietta		aggregate slurry, large
39	Counts	Counts	#	Quigg, Antonietta		aggregate slurry, medium
40	Counts	Counts	#	Quigg, Antonietta		aggregate slurry, small
41	Counts	Counts	#	Quigg, Antonietta		aggregate slurry, XS
42	Optical density	OD	arbitrary units	Quigg, Antonietta		aggregate slurry, large
43	Optical density	OD	arbitrary units	Quigg, Antonietta		aggregate slurry, medium
44	Optical density	OD	arbitrary units	Quigg, Antonietta		aggregate slurry, small
45	Optical density	OD	arbitrary units	Quigg, Antonietta		aggregate slurry, XS
46	Aspect ratio	Aspect ratio		Quigg, Antonietta		aggregate slurry, large
47	Aspect ratio	Aspect ratio		Quigg, Antonietta		aggregate slurry, medium
48	Aspect ratio	Aspect ratio		Quigg, Antonietta		aggregate slurry, small
49	Aspect ratio	Aspect ratio		Quigg, Antonietta		aggregate slurry, XS
50	Settling time	Settle time	s	Quigg, Antonietta		aggregate slurry tank
51	Salinity	Sal		Quigg, Antonietta
52	Temperature, water	Temp	°C	Quigg, Antonietta
53	Temperature, water, standard deviation	Temp std dev	±	Quigg, Antonietta
54	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1451 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Time point	6 Growth phase	7 Treat (OA=ocean acidification, WAF=w...)	8 Q	9 sigma PSII [A²/quanta]	10 p	11 Chl a [µg/l] (surrounding seawater)	12 Chl a [µg/l] (aggregate slurry)	13 Cells [#/ml]	14 CHO [mg] (surrounding seawater)	15 Protein [µg] (surrounding seawater)	16 CHO [mg] (aggregate slurry)	17 Protein [µg] (aggregate slurry)	18 TEP [µg Xeq/l]	19 PAH [mg/l] (surrounding seawater)	20 PAH mass [mg] (surrounding seawater)	21 PAH mass [mg] (aggregate slurry)	22 ESD [mm] (Large)	23 ESD [mm] (Medium)	24 ESD [mm] (Small)	25 ESD [mm] (XS)	26 Oil eq [mg/l] (Estimate)	27 pH (total scale)	28 AT [µmol/kg]	29 pCO2water_SST_wet [µatm]	30 DIC [µmol/kg]	31 [HCO3]- [µmol/kg]	32 [CO3]2- [µmol/kg]	33 CO2 [µmol/kg]	34 Area [cm²] (aggregate slurry, large)	35 Area [cm²] (aggregate slurry, medium)	36 Area [cm²] (aggregate slurry, small)	37 Area [cm²] (aggregate slurry, XS)	38 Counts [#] (aggregate slurry, large)	39 Counts [#] (aggregate slurry, medium)	40 Counts [#] (aggregate slurry, small)	41 Counts [#] (aggregate slurry, XS)	42 OD [arbitrary units] (aggregate slurry, large)	43 OD [arbitrary units] (aggregate slurry, medium)	44 OD [arbitrary units] (aggregate slurry, small)	45 OD [arbitrary units] (aggregate slurry, XS)	46 Aspect ratio (aggregate slurry, large)	47 Aspect ratio (aggregate slurry, medium)	48 Aspect ratio (aggregate slurry, small)	49 Aspect ratio (aggregate slurry, XS)	50 Settle time [s] (aggregate slurry tank)	51 Sal	52 Temp [°C]	53 Temp std dev [±]	54 CSC flag	55 CO2 [µmol/kg]	56 fCO2water_SST_wet [µatm]	57 pCO2water_SST_wet [µatm]	58 [HCO3]- [µmol/kg]	59 [CO3]2- [µmol/kg]	60 DIC [µmol/kg]	61 Omega Arg	62 Omega Cal
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	Control A	0.348	207	0.18	4.441060000															0.000000000	8.15	2420	327.7170715	2119.849121	1895.006419	213.7794159	11.06327590																		32	19	1	8	10.98	324.00	325.12	1897.57	215.99	2124.54	3.40	5.27
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	Control B																			0.000000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	Control C																			0.000000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OA A	0.351	214	0.23	4.655950000															0.000000000	7.90	2420	643.4392090	2246.732422	2092.278961	132.7318861	21.72161786																		32	19	1	8	21.55	636.30	638.49	2095.59	134.14	2251.28	2.11	3.27
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OA B																			0.000000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OA C																			0.000000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	WAF A	0.330	183	0.07	6.670544000															0.765522000	8.15	2420	327.7170715	2119.849121	1895.006419	213.7794159	11.06327590																		32	19	1	8	10.98	324.00	325.12	1897.57	215.99	2124.54	3.40	5.27
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	WAF B																			0.731733000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	WAF C																			0.736080000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OAWAF A	0.309	228	0.17	4.879794000															0.420702000	7.87	2420	695.6668091	2259.594238	2111.121335	124.9880990	23.48475239																		32	19	1	8	23.30	687.96	690.33	2114.51	126.31	2264.13	1.99	3.08
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OAWAF B																			0.511023000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OAWAF C																			0.544714000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	DCEWAF A
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	DCEWAF B
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	DCEWAF C
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OADCEWAF A
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OADCEWAF B
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Stationary	OADCEWAF C
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	Control A	0.418	104	0.08	0.601692000															0.000000000								0.0000	2.193555556	0.911176471	0.134928571	0	9	17	98	0.000	78.23933333	76.26088235	111.99303060	0.0000	1.925000000	1.845294118	1.718795918
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	Control B																			0.000000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	Control C	0.298	649	0.23	0.163555167															0.000000000								0.0000	2.033300000	0.944133333	0.164785714	0	10	15	14	0.000	87.46040000	88.72946667	100.73807690	0.0000	1.924000000	1.612733333	1.598500000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OA A	0.124	919	0.47	0.378922700															0.000000000								0.0000	2.409666667	1.001882353	0.126390244	0	6	17	41	0.000	77.16550000	97.88270588	114.89957500	0.0000	2.347666667	1.767705882	1.649024390
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OA B																			0.000000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OA C	0.406	358	0.04	0.320902400															0.000000000								0.0000	2.227400000	0.960272727	0.107328947	0	5	11	76	0.000	61.56640000	62.66372727	98.50233333	0.0000	1.755000000	1.660363636	1.644671053
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	WAF A	0.433	309	0.06	1.142498500															0.141871000								6.5370	2.749333333	1.028400000	0.094790598	2	3	5	234	66.641	74.58433333	83.15400000	112.14565240	1.7515	1.644000000	1.685000000	1.848935897
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	WAF B	0.282	374	0.05	0.757487250															0.099710000								6.0000	2.752000000	1.255666667	0.035428571	2	5	6	182	65.223	73.76760000	77.74083333	118.90505520	1.8080	1.762200000	1.362333333	1.759912088
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	WAF C
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OAWAF A	0.000	556	0.34	0.661861200															0.105219000								10.0320	0.000000000	0.747000000	0.063598291	1	0	2	117	37.989	0.00000000	68.67600000	107.43778450	1.2850	0.000000000	1.323000000	1.782786325
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OAWAF B	0.329	1313	0.19	0.573040000															0.096553000								6.0515	1.654000000	1.143000000	0.049603774	2	1	1	159	43.208	56.05300000	63.94700000	101.89756960	1.2815	1.321000000	1.404000000	1.741949686
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OAWAF C	0.084	183	0.10	0.893584250															0.167505000								8.0220	2.515000000	1.024000000	0.053900763	1	1	1	131	62.964	61.46300000	82.43600000	112.76317690	1.4690	1.922000000	1.344000000	1.811106870
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	DCEWAF A
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	DCEWAF B
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	DCEWAF C
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OADCEWAF A
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OADCEWAF B
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Stationary	OADCEWAF C
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	Control A	0.481	321	0.08	6.306330333		5608.000000	0.145771872	0.020087			1815.4800000	0.14913	0.850041						0.000000000	8.10	2450	381.0000000	2175.000000	1965.000000	197.0000000	12.00000000																		32	19	1	8	12.77	377.06	378.36	1968.14	199.66	2180.58	3.14	4.87
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	Control B						5191.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	Control C						5652.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OA A	0.462	326	0.13	6.131528000		3661.000000	0.007039534	0.021836			1632.6200000	0.08912	0.507984						0.000000000	7.90	2450	651.0000000	2275.000000	2118.000000	134.0000000	22.00000000																		32	19	1	8	21.83	644.37	646.59	2122.19	135.84	2279.85	2.14	3.32
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OA B						3698.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OA C						3619.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	WAF A	0.473	289	0.07	5.190284667		3426.333333	0.072588136	0.119144			892.1600000	61.27069	349.242933						0.263184533	8.10	2420	376.0000000	2148.000000	1940.000000	195.0000000	1.00000000																		32	19	1	8	12.61	372.28	373.56	1943.20	197.13	2152.95	3.10	4.81
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	WAF B						3323.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	WAF C						3254.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OAWAF A	0.450	297	0.06	5.580228333		4818.000000	0.036614304	0.150255			1005.5022220	60.29232	343.666224						0.194902400	7.90	2420	643.0000000	2246.000000	2092.000000	132.0000000	21.00000000																		32	19	1	8	21.55	636.30	638.49	2095.59	134.14	2251.28	2.11	3.27
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OAWAF B						4784.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OAWAF C						4730.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	DCEWAF A	0.508	278	0.06	6.588703333		5714.666667	0.047748699	0.168487				2.75176	15.685032						0.200000000	8.17	2485	318.0000000	2167.000000	1928.000000	227.0000000	10.00000000																		32	19	1	8	10.67	314.86	315.95	1930.94	230.15	2171.75	3.62	5.62
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	DCEWAF B						5501.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	DCEWAF C						5776.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OADCEWAF A	0.465	287	0.17	6.736613000		5234.000000	0.081206597	0.190376				2.56176	14.602032						0.200000000	7.99	2485	521.0000000	2266.000000	2086.000000	162.0000000	17.00000000																		32	19	1	8	17.46	515.57	517.35	2088.99	164.50	2270.96	2.59	4.02
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OADCEWAF B						5022.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	initial	Exponential	OADCEWAF C						5026.000000
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	Control A	0.532	327	0.08	0.472542571	2769.9446670	476.000000	0.027544209	0.050509	0.124496719	231.44301900	219.1040000	0.42971	2.449347	0.070580763		18.23346225		2.860175219	0.000000000									2.624000000		0.088140000	0	5	0	50		64.36380000		139.16862000		1.502400000		1.665120000	25
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	Control B	0.542	169	0.05	1.066102143	1653.8990000	1047.000000	0.036389630	0.079288	0.113790626	193.89941520	248.6240000	0.44398	2.530686	0.131802880	26.15796115	18.02598601	11.247933570	3.339893025	0.000000000								5.3740	2.582000000	1.009000000	0.106050000	1	2	8	40	53.893	55.57950000	73.89625000	111.37548720	1.7540	1.238000000	1.481125000	1.484051282	27
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	Control C	0.261	491	0.14	0.548418310	2285.8766670	648.000000	0.022318040	0.079288	0.086981452	177.96648390	247.0933333	0.35881	2.045217	0.009605390		18.40204744	11.435000370	2.718438154	0.000000000									2.664800000	1.034800000	0.076311111	0	5	5	45		46.09540000	65.96880000	118.53442220		1.205000000	1.450400000	1.616688889	40
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OA A	0.532	455	0.13	0.677118929	3025.4250000	981.000000	0.019358040	0.061268	0.107149687	307.22565790	241.0800000	0.22506	1.282842	0.005770048	25.36720848	19.04632358	9.810239535	2.801837024	0.000000000								5.0540	2.868333333	0.764500000	0.076500000	1	3	2	48	52.258	64.45133333	104.26600000	114.78868750	1.3560	1.455000000	1.410000000	1.718208333	57
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OA B	0.232	845	0.05	0.748192405	2958.1933330	819.000000	0.020218787	0.059466	0.077447108	230.16644740	214.4755556	0.22441	1.279137	0.013078260	35.56095493	16.33618709	9.554646794	2.590637741	0.000000000								9.9320	2.096000000	0.717000000	0.069195652	1	1	1	46	37.426	49.28300000	87.43600000	113.14854350	1.4220	1.822000000	1.283000000	1.720891304	27
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OA C	0.431	93	0.06	0.260282595	2729.6056670	537.000000	0.044031124	0.048707	0.209207592	343.23505120	176.5733333	0.19824	1.129968	0.018969864	26.51330897	15.82352446	11.026825820	2.392577675	0.000000000								5.5210	1.990750000	0.975285714	0.060625000	1	5	7	64	54.211	64.38150000	59.59142857	113.82598440	1.4500	1.212000000	1.351857143	1.724218750	75
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	WAF A	0.283	233	0.11	2.410735476	1085.1191000	3155.000000	0.028744209	0.077486	0.073902327	86.54729532	595.1377778	49.63105	282.896985	1.024102174		16.22278071	11.123529180	3.622224933	0.132104533									2.067000000	0.973750000	0.117946429	0	1	4	56		66.53400000	89.28900000	114.93971430		1.748000000	1.751000000	1.794821429	210
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	WAF B	0.171	236	0.57	3.937854762	714.0003000	2776.000000	0.036069630	0.090100	0.089671790	92.37419591	740.9155556	36.55312	208.352784	0.802842739		15.73674336	10.021433310	3.548625027	0.124390400									1.945000000	0.795000000	0.110682540	0	1	5	63		68.11900000	105.88900000	116.07141270		1.684000000	2.093400000	1.691269841	105
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	WAF C	0.550	634	0.10	2.381921905	1337.9101670	951.000000	0.057841967	0.113685	0.101291078	172.07759500	697.5466667	37.17314	211.886898	2.658703707		21.05266457	9.757229876	3.711893222	0.143163467									3.481000000	0.753333333	0.124540230	0	1	9	87		80.92900000	79.41566667	109.45047130		2.436000000	1.789000000	1.701563218	120
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OAWAF A	0.546	449	0.08	1.719209762	1895.9330000	897.000000	0.045775799	0.099163	0.074806792	225.40109650	576.1866667	40.43610	230.485770	2.035384575		15.06850872	10.482281070	3.445183736	0.124390400									1.786500000	0.872727273	0.118340426	0	2	11	47		58.28600000	77.69327273	107.40868090		1.845500000	1.425000000	1.759489362	120
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OAWAF B	0.500	567	0.11	1.120847929	2191.7523330	228.000000	0.042629630	0.095559	0.076594410	280.48739040	704.6533333	38.32920	218.476440	2.550902025	24.41839632	17.68354128	9.781297180	3.413934607	0.096245867								4.6830	2.456000000	0.751500000	0.104746479	1	1	2	71	58.325	61.65400000	100.97600000	113.36645070	1.6180	1.844000000	1.667500000	1.879014085	180
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OAWAF C	0.533	567	0.11	2.209040476	712.6556667	1960.000000	0.039330377	0.088298	0.150219258	150.93377190	774.0800000	32.29906	184.104642	2.582542590		15.48433633	10.780388280	3.992612567	0.123154933									1.884000000	0.924857143	0.146931034	0	2	7	58		68.66500000	95.60757143	113.04686210		2.655500000	1.605428571	1.827258621	170
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	DCEWAF A	0.373	216	0.32	0.582034143	1668.6899670	1339.000000	0.039205519	0.061904	0.115354169	309.51922510		2.13007	12.141399	1.269752406		18.08186069	11.076572120	3.336024911	0.068704000									2.618000000	0.983285714	0.098187500	0	6	7	48		78.11233333	89.88814286	112.28570830		1.681333333	1.516285714	1.903791667	70
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	DCEWAF B	0.368	72	0.19	0.616610429	2568.2496670	1085.000000	0.060437109	0.081408	0.118554183	313.84194080		2.24460	12.794220	1.429317698	36.33486045	19.11325362	12.408196560	3.426740328	0.129844533								10.3690	2.878000000	1.215500000	0.107909091	1	2	4	44	49.278	63.39600000	80.09450000	107.81695450	1.5450	1.388000000	1.467250000	1.543681818	78
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	DCEWAF C	0.533	723	0.16	0.781808238	1909.3793330	229.000000	0.033001314	0.100965	0.151266564	337.06334060		1.85811	10.591227	1.432023455	24.99811930	18.06609853	11.127523110	3.217117399	0.350978000								4.9080	2.617600000	0.984000000	0.096000000	1	5	3	54	56.484	72.81320000	95.84433333	115.72064810	1.6490	1.894000000	1.459333333	1.733722222	51
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OADCEWAF A	0.518	440	0.19	0.797175476	2191.7523330	239.000000	0.058295007	0.100965	0.118741267	330.97686400		2.07691	11.838387	1.289060513	27.12150848	17.14873200	12.791062560	3.790069537	0.102528667								5.8020	2.351500000	1.285000000	0.125966102	2	2	1	59	74.225	83.65800000	95.73600000	116.81816950	1.8305	1.260000000	1.457000000	1.776000000	61
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OADCEWAF B	0.457	560	0.15	0.698248881	1721.1306670	1067.000000	0.037187622	0.120522	0.119274218	337.95732820		2.06674	11.780418	1.292536988	32.71132088	15.31717824	11.400275640	4.245969750	0.000000000								8.4040	1.849000000	1.042333333	0.163387755	1	3	6	49	63.201	68.61066667	85.83566667	106.45322450	1.4120	1.288333333	1.975333333	1.615163265	98
laboratory	Thalassiosira pseudonana	marinespecies.org	urn:lsid:marinespecies.org:taxname:148934	final	Exponential	OADCEWAF C	0.505	1734	0.53	1.680791667	2164.8596670	2005.000000	0.038085519	0.142305	0.098749090	280.30142540		2.40866	13.729362	0.992877139	25.86287361	21.41246273	9.908191993	3.439301913	0.065630400								5.2655	3.601000000	0.779500000	0.110235632	2	1	2	174	80.884	62.88500000	110.19150000	113.62406320	2.5275	1.922000000	2.433000000	1.699718391	70