/* DATA DESCRIPTION: Citation: Crovetto, Lucas; Venn, Alexander A; Sevilgen, Duygu; Tambutté, Sylvie; Tambutté, Eric (2024): Measurements of the coelenteron pH in a reef coral, Stylophora pistillata, using microsensors, under light and dark conditions and in tissues with different dinoflagellate densities [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.964874 Abstract: pH measurements in the cœlenteron (pHcoel) of a reef coral, Stylophora pistillata, were performed using the microsensor technique. Seven samples grown in long-term coral culture facilities on glass slides at the Centre Scientifique de Monaco were used for this study. Measuremetns were made using motorised micromanipulator for precise movements of the microsensor on the order of micrometres. We first performed depth profiles in the polyps and in the coenosarc in tissue with a high dinoflagellate density, under light conditions to determine the variation of pHcoel. We then measured pHcoel of polyps and coenosarc at eight light intensities, from darkness to strong illumination, which allowed us to derive a pHcoel-irradiance curve and evaluate the role of photosynthesis in influencing pH in the coelenteron. Finally, we measured pHcoel in the coenosarc under light and dark conditions in two regions of interest characterised by visually different densities of dinoflagellates residing within the coral tissue: a high dinoflagellate density (HDD) tissue at the centre of the microcolonies and a low dinoflagellate density (LDD) tissue at the edge of microcolonies. For each sample, at least three replicate measurements were performed under all conditions. Keyword(s): calcification; coenosarc; Dinoflagellate; Laboratory experiment; Photosynthesis; polyp Supplement to: Crovetto, Lucas; Venn, Alexander A; Sevilgen, Duygu; Tambutté, Sylvie; Tambutté, Eric (2024): Spatial variability of and effect of light on the cœlenteron pH of a reef coral. Communications Biology, 7, 246, https://doi.org/10.1038/s42003-024-05938-8 Comment: Stylophora pistillata is a species cultured for many years at the Centre Scientifique de Monaco. We use nubbins to make our experiments. Originally collected in the Red Sea 30 years ago. Samples are maintained in long-term coral culture facilities supplied with flowing seawater directly from the Mediterranean Sea (exchange rate 170% h-1), at a salinity of 38, temperature of 25°C, under an irradiance of 175 µmol photons m-2 s-1 (provided by a BLV HQI Light Bulb Nepturion, 150W) on a 12h: 12h photoperiod. Parameter(s): Type of study (Study type) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) Date/time start, experiment (Date/time start exp) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) Date/time end, experiment (Date/time end exp) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) Figure (Fig) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * COMMENT: refers to the figures in the manuscript Treatment: light intensity [µmol/m**2/s] (T:Io) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: Light bulb, Nepturion, BLV HQI 150W * COMMENT: photons Depth in coral tissue [µm] (Depth cor tis) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) Temperature, water [°C] (Temp) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: Thermometer, Ponsel Salinity (Sal) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: Portable 2-channel multimeter, Hach, HQ40D pH (pH) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] * COMMENT: seawater pH, standard deviation [±] (pH std dev) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] * COMMENT: seawater Stylophora pistillata, polyp, pH (S. pistillata polyp pH) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, polyp, pH, standard error [±] (S. pistillata polyp pH std e) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coenosarc, pH (S. pistillata coenosarc pH) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coenosarc, pH, standard error [±] (S. pistillata coenosarc pH std e) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in polyp, pH (S. pistillata coel polyp pH) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in polyp, pH, standard deviation [±] (S. pistillata coel polyp pH std dev) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc, pH (S. pistillata cœl coenosarc pH) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc, pH, standard deviation [±] (S. pistillata cœl coenosarc pH std dev) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc in high dinoflagellate density tissues, pH (S. pistillata cœl coenosarc HDD pH) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc in high dinoflagellate density tissues, pH, standard deviation [±] (S. pistillata coel coenosarc HDD pH std) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc in low dinoflagellate density tissues, pH (S. pistillata coel coenosarc LDD pH) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc in low dinoflagellate density tissues, pH, standard deviation [±] (S. pistillata coel coenosarc LDD pH std) * PI: Crovetto, Lucas (https://orcid.org/0009-0008-5375-1058, lcrovetto@centrescientifique.mc) * METHOD/DEVICE: pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] License: Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 439 data points */ Study type Date/time start exp Date/time end exp Fig T:Io [µmol/m**2/s] Depth cor tis [µm] Temp [°C] Sal pH pH std dev [±] S. pistillata polyp pH S. pistillata polyp pH std e [±] S. pistillata coenosarc pH S. pistillata coenosarc pH std e [±] S. pistillata coel polyp pH S. pistillata coel polyp pH std dev [±] S. pistillata cœl coenosarc pH S. pistillata cœl coenosarc pH std dev [±] S. pistillata cœl coenosarc HDD pH S. pistillata coel coenosarc HDD pH std [±] S. pistillata coel coenosarc LDD pH S. pistillata coel coenosarc LDD pH std [±] Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 0 25 38 8.08 0.04 8.48 0.03 8.37 0.03 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 25 25 38 8.08 0.04 8.48 0.01 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 50 25 38 8.08 0.04 8.52 0.02 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 75 25 38 8.08 0.04 8.62 0.04 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 100 25 38 8.08 0.04 8.53 0.04 8.73 0.03 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 125 25 38 8.08 0.04 8.68 0.03 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 150 25 38 8.08 0.04 8.72 0.03 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 175 25 38 8.08 0.04 8.70 0.02 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 200 25 38 8.08 0.04 8.53 0.04 8.70 0.02 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 225 25 38 8.08 0.04 8.69 0.02 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 250 25 38 8.08 0.04 8.71 0.02 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 275 25 38 8.08 0.04 8.72 0.07 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 300 25 38 8.08 0.04 8.56 0.04 8.67 0.03 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 400 25 38 8.08 0.04 8.69 0.04 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 500 25 38 8.08 0.04 8.64 0.05 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 600 25 38 8.08 0.04 8.65 0.05 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 700 25 38 8.08 0.04 8.65 0.05 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 800 25 38 8.08 0.04 8.71 0.05 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 900 25 38 8.08 0.04 8.66 0.05 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 1000 25 38 8.08 0.04 8.68 0.05 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 1100 25 38 8.08 0.04 8.67 0.05 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 1200 25 38 8.08 0.04 8.71 0.09 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 1300 25 38 8.08 0.04 8.65 0.15 Laboratory experiment 2021-10-04 2023-02-23 3a/3b 250 1400 25 38 8.08 0.04 8.68 0.12 Laboratory experiment 2021-10-04 2023-02-23 3c 250 25 38 8.08 0.04 8.67 0.27 8.66 0.18 Laboratory experiment 2021-10-04 2023-02-23 4 0 25 38 8.08 0.04 7.89 0.04 7.63 0.23 Laboratory experiment 2021-10-04 2023-02-23 4 50 25 38 8.08 0.04 8.65 0.01 8.11 0.24 Laboratory experiment 2021-10-04 2023-02-23 4 100 25 38 8.08 0.04 8.76 0.04 8.45 0.24 Laboratory experiment 2021-10-04 2023-02-23 4 150 25 38 8.08 0.04 8.78 0.04 8.71 0.05 Laboratory experiment 2021-10-04 2023-02-23 4 200 25 38 8.08 0.04 8.74 0.10 8.75 0.13 Laboratory experiment 2021-10-04 2023-02-23 4 250 25 38 8.08 0.04 8.75 0.10 8.77 0.11 Laboratory experiment 2021-10-04 2023-02-23 4 300 25 38 8.08 0.04 8.81 0.13 8.79 0.12 Laboratory experiment 2021-10-04 2023-02-23 4 350 25 38 8.08 0.04 8.81 0.14 8.76 0.13 Laboratory experiment 2021-10-04 2023-02-23 5 250 25 38 8.08 0.04 8.80 0.13 7.67 0.14 Laboratory experiment 2021-10-04 2023-02-23 5 0 25 38 8.08 0.04 7.63 0.23 7.69 0.09