Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 Venn Alexander A avenn@centrescientifique.mc 0000-0003-0544-0884 Sevilgen Duygu duygu.sevilgen@gmail.com 0000-0002-5026-9214 Tambutté Sylvie stambutté@centrescientifique.mc 0000-0002-0505-6375 Tambutté Eric etambutté@centrescientifique.mc 0000-0002-1419-3785 2024 Measurements of the coelenteron pH in a reef coral, Stylophora pistillata, using microsensors, under light and dark conditions and in tissues with different dinoflagellate densities dataset https://doi.org/10.1594/PANGAEA.964874 2024-05-31T08:45:52 pH measurements in the cœlenteron (pHcoel) of a reef coral, Stylophora pistillata, were performed using the microsensor technique. Seven samples grown in long-term coral culture facilities on glass slides at the Centre Scientifique de Monaco were used for this study. Measuremetns were made using motorised micromanipulator for precise movements of the microsensor on the order of micrometres. We first performed depth profiles in the polyps and in the coenosarc in tissue with a high dinoflagellate density, under light conditions to determine the variation of pHcoel. We then measured pHcoel of polyps and coenosarc at eight light intensities, from darkness to strong illumination, which allowed us to derive a pHcoel-irradiance curve and evaluate the role of photosynthesis in influencing pH in the coelenteron. Finally, we measured pHcoel in the coenosarc under light and dark conditions in two regions of interest characterised by visually different densities of dinoflagellates residing within the coral tissue: a high dinoflagellate density (HDD) tissue at the centre of the microcolonies and a low dinoflagellate density (LDD) tissue at the edge of microcolonies. For each sample, at least three replicate measurements were performed under all conditions. Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 Venn Alexander A avenn@centrescientifique.mc 0000-0003-0544-0884 Sevilgen Duygu duygu.sevilgen@gmail.com 0000-0002-5026-9214 Tambutté Sylvie stambutté@centrescientifique.mc 0000-0002-0505-6375 Tambutté Eric etambutté@centrescientifique.mc 0000-0002-1419-3785 2024 Spatial variability of and effect of light on the cœlenteron pH of a reef coral journal article Communications Biology 7 https://doi.org/10.1038/s42003-024-05938-8 246 uncoded table 439 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Stylophora pistillata is a species cultured for many years at the Centre Scientifique de Monaco. We use nubbins to make our experiments. Originally collected in the Red Sea 30 years ago. Samples are maintained in long-term coral culture facilities supplied with flowing seawater directly from the Mediterranean Sea (exchange rate 170% h-1), at a salinity of 38, temperature of 25°C, under an irradiance of 175 µmol photons m-2 s-1 (provided by a BLV HQI Light Bulb Nepturion, 150W) on a 12h: 12h photoperiod. Type of study Study type type Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 Study type Date/time start, experiment Date/time start exp Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 Date/time start exp Date/time end, experiment Date/time end exp Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 Date/time end exp Figure Fig Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 refers to the figures in the manuscript Fig Treatment: light intensity T:Io µmol/m**2/s treatment Molar Flux Light bulb, Nepturion, BLV HQI 150W Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 photons T:Io [µmol/m**2/s] Depth in coral tissue Depth cor tis µm depth http://purl.obolibrary.org/obo/PATO_0001595 Length L http://dbpedia.org/resource/Length Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 Depth cor tis [µm] Temperature, water Temp °C Temperature Θ http://dbpedia.org/resource/Temperature water http://purl.obolibrary.org/obo/CHEBI_15377 Thermometer, Ponsel Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 Temp [°C] Salinity Sal salinity http://purl.obolibrary.org/obo/PATO_0085001 Portable 2-channel multimeter, Hach, HQ40D Hach HQ40D - Multi 2 Channels Portable Two Channel Multi Meter http://vocab.nerc.ac.uk/collection/L22/current/TOOL1481/ Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 Sal pH pH pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 seawater pH pH, standard deviation pH std dev ± standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 seawater pH std dev [±] Stylophora pistillata, polyp, pH S. pistillata polyp pH Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata polyp pH Stylophora pistillata, polyp, pH, standard error S. pistillata polyp pH std e ± Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 standard error std e https://en.wikipedia.org/wiki/Standard_error pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata polyp pH std e [±] Stylophora pistillata, coenosarc, pH S. pistillata coenosarc pH Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata coenosarc pH Stylophora pistillata, coenosarc, pH, standard error S. pistillata coenosarc pH std e ± Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 standard error std e https://en.wikipedia.org/wiki/Standard_error pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata coenosarc pH std e [±] Stylophora pistillata, coelenteron in polyp, pH S. pistillata coel polyp pH Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata coel polyp pH Stylophora pistillata, coelenteron in polyp, pH, standard deviation S. pistillata coel polyp pH std dev ± Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata coel polyp pH std dev [±] Stylophora pistillata, coelenteron in coenosarc, pH S. pistillata cœl coenosarc pH Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata cœl coenosarc pH Stylophora pistillata, coelenteron in coenosarc, pH, standard deviation S. pistillata cœl coenosarc pH std dev ± Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata cœl coenosarc pH std dev [±] Stylophora pistillata, coelenteron in coenosarc in high dinoflagellate density tissues, pH S. pistillata cœl coenosarc HDD pH Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata cœl coenosarc HDD pH Stylophora pistillata, coelenteron in coenosarc in high dinoflagellate density tissues, pH, standard deviation S. pistillata coel coenosarc HDD pH std ± Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata coel coenosarc HDD pH std [±] Stylophora pistillata, coelenteron in coenosarc in low dinoflagellate density tissues, pH S. pistillata coel coenosarc LDD pH Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata coel coenosarc LDD pH Stylophora pistillata, coelenteron in coenosarc in low dinoflagellate density tissues, pH, standard deviation S. pistillata coel coenosarc LDD pH std ± Stylophora pistillata https://www.marinespecies.org/aphia.php?p=taxdetails&id=206982 standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Crovetto Lucas lcrovetto@centrescientifique.mc 0009-0008-5375-1058 S. pistillata coel coenosarc LDD pH std [±] CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable calcification coenosarc Dinoflagellate Laboratory experiment Photosynthesis polyp author104082 author39320 author39321 author48620 author51127 curlevel30 journal17027 license21 method13926 method16329 method16330 method16350 param130782 param185520 param50001 param516119 param516120 param525717 param525718 param525719 param525720 param525721 param525722 param525723 param525724 param526795 param526796 param526797 param526798 param526799 param716 param717 param764 param87459 pi104082 ref124148 term1055375 term1073131 term2606205 term2664259 term2698012 term37764 term38527 term38841 term41013 term43863 term43972 term44029 term47898 term47899 term49506 term50622 term73642 term73643 topotype11