de.pangaea.dataset964874 und dataset PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact 2026-07-15T18:25:53Z https://doi.pangaea.de/10.1594/PANGAEA.964874 <gco:CharacterString>Measurements of the coelenteron pH in a reef coral, Stylophora pistillata, using microsensors, under light and dark conditions and in tissues with different dinoflagellate densities</gco:CharacterString> 2024-05-31T08:45:52 publication https://doi.org/10.1594/PANGAEA.964874 Crovetto, Lucas
lcrovetto@centrescientifique.mc
https://orcid.org/0009-0008-5375-1058 information author Venn, Alexander A
avenn@centrescientifique.mc
https://orcid.org/0000-0003-0544-0884 information author Sevilgen, Duygu
duygu.sevilgen@gmail.com
https://orcid.org/0000-0002-5026-9214 information author Tambutté, Sylvie
stambutté@centrescientifique.mc
https://orcid.org/0000-0002-0505-6375 information author Tambutté, Eric
etambutté@centrescientifique.mc
https://orcid.org/0000-0002-1419-3785 information author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital pH measurements in the cœlenteron (pHcoel) of a reef coral, Stylophora pistillata, were performed using the microsensor technique. Seven samples grown in long-term coral culture facilities on glass slides at the Centre Scientifique de Monaco were used for this study. Measuremetns were made using motorised micromanipulator for precise movements of the microsensor on the order of micrometres. We first performed depth profiles in the polyps and in the coenosarc in tissue with a high dinoflagellate density, under light conditions to determine the variation of pHcoel. We then measured pHcoel of polyps and coenosarc at eight light intensities, from darkness to strong illumination, which allowed us to derive a pHcoel-irradiance curve and evaluate the role of photosynthesis in influencing pH in the coelenteron. Finally, we measured pHcoel in the coenosarc under light and dark conditions in two regions of interest characterised by visually different densities of dinoflagellates residing within the coral tissue: a high dinoflagellate density (HDD) tissue at the centre of the microcolonies and a low dinoflagellate density (LDD) tissue at the edge of microcolonies. For each sample, at least three replicate measurements were performed under all conditions. completed Crovetto, Lucas
lcrovetto@centrescientifique.mc
https://orcid.org/0009-0008-5375-1058 information principalInvestigator calcification coenosarc Dinoflagellate Laboratory experiment Photosynthesis polyp theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation Stylophora pistillata is a species cultured for many years at the Centre Scientifique de Monaco. We use nubbins to make our experiments. Originally collected in the Red Sea 30 years ago. Samples are maintained in long-term coral culture facilities supplied with flowing seawater directly from the Mediterranean Sea (exchange rate 170% h-1), at a salinity of 38, temperature of 25°C, under an irradiance of 175 µmol photons m-2 s-1 (provided by a BLV HQI Light Bulb Nepturion, 150W) on a 12h: 12h photoperiod. Type of study thematicClassification Date/time start, experiment thematicClassification Date/time end, experiment thematicClassification Figure thematicClassification refers to the figures in the manuscript Treatment: light intensity physicalMeasurement Light bulb, Nepturion, BLV HQI 150W; photons µmol/m**2/s µmol/m**2/s Depth in coral tissue physicalMeasurement µm µm Temperature, water physicalMeasurement Thermometer, Ponsel °C °C Salinity thematicClassification Portable 2-channel multimeter, Hach, HQ40D pH thematicClassification pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO]; seawater pH, standard deviation physicalMeasurement pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO]; seawater ± ± Stylophora pistillata, polyp, pH thematicClassification pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, polyp, pH, standard error physicalMeasurement pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] ± ± Stylophora pistillata, coenosarc, pH thematicClassification pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coenosarc, pH, standard error physicalMeasurement pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] ± ± Stylophora pistillata, coelenteron in polyp, pH thematicClassification pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in polyp, pH, standard deviation physicalMeasurement pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] ± ± Stylophora pistillata, coelenteron in coenosarc, pH thematicClassification pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc, pH, standard deviation physicalMeasurement pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] ± ± Stylophora pistillata, coelenteron in coenosarc in high dinoflagellate density tissues, pH thematicClassification pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc in high dinoflagellate density tissues, pH, standard deviation physicalMeasurement pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] ± ± Stylophora pistillata, coelenteron in coenosarc in low dinoflagellate density tissues, pH thematicClassification pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Stylophora pistillata, coelenteron in coenosarc in low dinoflagellate density tissues, pH, standard deviation physicalMeasurement pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] ± ± text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.964874?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. Supplement to <gco:CharacterString>Spatial variability of and effect of light on the cœlenteron pH of a reef coral</gco:CharacterString> 2024-01-01 publication https://doi.org/10.1038/s42003-024-05938-8 Crovetto, Lucas
lcrovetto@centrescientifique.mc
https://orcid.org/0009-0008-5375-1058 information author Venn, Alexander A
avenn@centrescientifique.mc
https://orcid.org/0000-0003-0544-0884 information author Sevilgen, Duygu
duygu.sevilgen@gmail.com
https://orcid.org/0000-0002-5026-9214 information author Tambutté, Sylvie
stambutté@centrescientifique.mc
https://orcid.org/0000-0002-0505-6375 information author Tambutté, Eric
etambutté@centrescientifique.mc
https://orcid.org/0000-0002-1419-3785 information author documentDigital Communications Biology 7 246