PANGAEA_964874 Measurements of the coelenteron pH in a reef coral, Stylophora pistillata, using microsensors, under light and dark conditions and in tissues with different dinoflagellate densities Crovetto, Lucas; Venn, Alexander A; Sevilgen, Duygu; Tambutté, Sylvie; Tambutté, Eric Measurements of the coelenteron pH in a reef coral, Stylophora pistillata, using microsensors, under light and dark conditions and in tissues with different dinoflagellate densities 2024-05-31 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.964874 Investigator Lucas Crovetto lcrovetto@centrescientifique.mc Earth Science Type of study Date/time start, experiment Date/time end, experiment Figure Treatment: light intensity Depth in coral tissue Temperature, water Salinity pH pH, standard deviation Stylophora pistillata, polyp, pH Stylophora pistillata, polyp, pH, standard error Stylophora pistillata, coenosarc, pH Stylophora pistillata, coenosarc, pH, standard error Stylophora pistillata, coelenteron in polyp, pH Stylophora pistillata, coelenteron in polyp, pH, standard deviation Stylophora pistillata, coelenteron in coenosarc, pH Stylophora pistillata, coelenteron in coenosarc, pH, standard deviation Stylophora pistillata, coelenteron in coenosarc in high dinoflagellate density tissues, pH Stylophora pistillata, coelenteron in coenosarc in high dinoflagellate density tissues, pH, standard deviation Stylophora pistillata, coelenteron in coenosarc in low dinoflagellate density tissues, pH Stylophora pistillata, coelenteron in coenosarc in low dinoflagellate density tissues, pH, standard deviation geoscientificInformation calcification coenosarc Dinoflagellate Laboratory experiment Photosynthesis polyp Light bulb, Nepturion, BLV HQI 150W Thermometer, Ponsel Portable 2-channel multimeter, Hach, HQ40D pH sensor, Mettler Toledo, InLab Pure Pro-ISM; mounted on Motorized micromanipulator, PyroScience, MUX2 [software Profix , PyroScience, version 4.51; Macroscope, Leica Microsystems, Z16 APO] Complete unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

Leobener Str.

Bremen Bremen 28359 Germany online 439 data points text/tab-separated-values Crovetto, Lucas; Venn, Alexander A; Sevilgen, Duygu; Tambutté, Sylvie; Tambutté, Eric (2024): Spatial variability of and effect of light on the cœlenteron pH of a reef coral. Communications Biology, 7, 246, https://doi.org/10.1038/s42003-024-05938-8

pH measurements in the cœlenteron (pHcoel) of a reef coral, Stylophora pistillata, were performed using the microsensor technique. Seven samples grown in long-term coral culture facilities on glass slides at the Centre Scientifique de Monaco were used for this study. Measuremetns were made using motorised micromanipulator for precise movements of the microsensor on the order of micrometres. We first performed depth profiles in the polyps and in the coenosarc in tissue with a high dinoflagellate density, under light conditions to determine the variation of pHcoel. We then measured pHcoel of polyps and coenosarc at eight light intensities, from darkness to strong illumination, which allowed us to derive a pHcoel-irradiance curve and evaluate the role of photosynthesis in influencing pH in the coelenteron. Finally, we measured pHcoel in the coenosarc under light and dark conditions in two regions of interest characterised by visually different densities of dinoflagellates residing within the coral tissue: a high dinoflagellate density (HDD) tissue at the centre of the microcolonies and a low dinoflagellate density (LDD) tissue at the edge of microcolonies. For each sample, at least three replicate measurements were performed under all conditions. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.964874"! ** Stylophora pistillata is a species cultured for many years at the Centre Scientifique de Monaco. We use nubbins to make our experiments. Originally collected in the Red Sea 30 years ago. Samples are maintained in long-term coral culture facilities supplied with flowing seawater directly from the Mediterranean Sea (exchange rate 170% h-1), at a salinity of 38, temperature of 25°C, under an irradiance of 175 µmol photons m-2 s-1 (provided by a BLV HQI Light Bulb Nepturion, 150W) on a 12h: 12h photoperiod.

https://doi.org/10.1038/s42003-024-05938-8 Spatial variability of and effect of light on the cœlenteron pH of a reef coral DIF 9.4 2024-05-31 2026-07-15